##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3133209
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11529617079486	33.0	31.0	34.0	30.0	34.0
2	32.439216471036566	34.0	31.0	34.0	30.0	34.0
3	32.49886585925165	34.0	31.0	34.0	31.0	34.0
4	35.988717956574234	37.0	35.0	37.0	35.0	37.0
5	30.462547503214754	37.0	35.0	37.0	0.0	37.0
6	32.93270988306238	37.0	35.0	37.0	17.0	37.0
7	35.14882505444099	37.0	35.0	37.0	32.0	37.0
8	35.74478529839535	37.0	35.0	37.0	35.0	37.0
9	37.66999552216274	39.0	38.0	39.0	35.0	39.0
10	37.504055746041836	39.0	37.0	39.0	35.0	39.0
11	37.340977891995074	39.0	37.0	39.0	35.0	39.0
12	37.08893597586372	39.0	37.0	39.0	34.0	39.0
13	36.987054167149395	39.0	37.0	39.0	33.0	39.0
14	37.89757561656436	40.0	37.0	41.0	33.0	41.0
15	38.08139897466144	40.0	37.0	41.0	33.0	41.0
16	38.26606236609176	40.0	37.0	41.0	33.0	41.0
17	38.32278631907415	40.0	37.0	41.0	34.0	41.0
18	38.29882111279522	40.0	37.0	41.0	34.0	41.0
19	38.285132590899615	40.0	37.0	41.0	34.0	41.0
20	38.196913771152836	40.0	37.0	41.0	34.0	41.0
21	38.16918947953999	40.0	37.0	41.0	34.0	41.0
22	38.212107459157686	40.0	36.0	41.0	34.0	41.0
23	38.17205906149255	40.0	36.0	41.0	34.0	41.0
24	38.14286183909213	40.0	36.0	41.0	34.0	41.0
25	38.082261987629934	40.0	36.0	41.0	34.0	41.0
26	38.03625548120154	40.0	36.0	41.0	34.0	41.0
27	38.01180195767343	40.0	36.0	41.0	34.0	41.0
28	37.96809724470982	40.0	36.0	41.0	34.0	41.0
29	37.95827313147639	40.0	36.0	41.0	34.0	41.0
30	37.843586559339	40.0	36.0	41.0	34.0	41.0
31	33.216139108498666	39.0	34.0	41.0	9.0	41.0
32	34.1538563179156	39.0	34.0	41.0	16.0	41.0
33	36.13298602167937	38.0	34.0	41.0	30.0	41.0
34	36.93496475977185	38.0	35.0	41.0	31.0	41.0
35	37.087889444974785	39.0	35.0	41.0	32.0	41.0
36	37.285794212898026	39.0	35.0	41.0	32.0	41.0
37	37.41171240092825	39.0	35.0	41.0	33.0	41.0
38	37.40269608570638	39.0	35.0	41.0	33.0	41.0
39	37.40141784349528	39.0	35.0	41.0	33.0	41.0
40	37.347004939664096	39.0	35.0	41.0	33.0	41.0
41	37.259591045474465	39.0	35.0	41.0	33.0	41.0
42	37.21380507971221	39.0	35.0	41.0	33.0	41.0
43	37.23001721238513	39.0	35.0	41.0	33.0	41.0
44	37.17315665823761	39.0	35.0	41.0	33.0	41.0
45	37.13119552509903	39.0	35.0	41.0	33.0	41.0
46	37.10818876110722	39.0	35.0	41.0	33.0	41.0
47	37.015089641323	39.0	35.0	41.0	33.0	41.0
48	36.99578451357697	39.0	35.0	41.0	33.0	41.0
49	36.966222170305265	39.0	35.0	41.0	33.0	41.0
50	36.77742052955931	39.0	35.0	41.0	32.0	41.0
51	36.13911583938384	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	13.0
10	20.0
11	29.0
12	32.0
13	52.0
14	59.0
15	98.0
16	168.0
17	315.0
18	578.0
19	873.0
20	1501.0
21	2477.0
22	3932.0
23	6111.0
24	8857.0
25	12062.0
26	15799.0
27	20048.0
28	25587.0
29	33637.0
30	46052.0
31	69061.0
32	101209.0
33	123117.0
34	243528.0
35	278069.0
36	349710.0
37	355472.0
38	524152.0
39	909741.0
40	849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80464820572136	20.75830243051134	26.562575302190183	10.874474061577125
2	31.171715643610113	23.857457322508647	26.758827770506212	18.211999263375027
3	32.29344100569097	23.401279646522145	26.95619730442495	17.349082043361932
4	28.473778799945997	25.876760854446673	26.445060000785137	19.204400344822194
5	22.84220427044605	39.205587625977074	21.228778546212528	16.72342955736435
6	28.115775232357628	32.8738044605387	23.236624176682756	15.77379613042092
7	83.04272073774843	5.3235835847528845	6.991649775038946	4.6420459024597465
8	85.0169905678172	3.7067428313910753	6.5214289886183785	4.7548376121733344
9	78.98068721237556	5.927756495018366	8.95991936701318	6.131636925592899
10	43.71278775210974	25.25580004398047	15.98042135076211	15.050990853147683
11	32.64100798893403	23.71986675641491	24.15794796963752	19.481177285013544
12	29.307269320367713	21.928955265990872	28.786301839424056	19.97747357421736
13	27.327095000684604	21.974659207221734	31.30956153898447	19.388684253109194
14	21.818525352123014	24.302847336388986	32.92601929842535	20.952608013062644
15	20.460173579228197	24.05939086731846	34.91800259733711	20.562432956116236
16	26.68053742983631	24.534494826230873	28.767120227217525	20.01784751671529
17	27.09455385836055	23.759666208031447	26.439953415172752	22.705826518435252
18	27.40321504246924	22.93721867899652	28.778737709485707	20.88082856904854
19	26.407941506615103	25.7751078846001	26.49306190554157	21.323888703243224
20	24.884200192199117	28.44001150258409	26.998518132687604	19.677270172529187
21	24.00095237821671	25.648528393733073	30.48111377185499	19.86940545619523
22	22.795606676733023	22.84485331173248	30.676919413929937	23.682620597604565
23	21.85021809907989	24.76914243512003	30.97862287514175	22.40201659065833
24	23.232060165791683	25.246065615156855	28.932701265699162	22.5891729533523
25	24.390552944281726	25.345931280039093	26.905993184623178	23.357522591056007
26	21.561249185738966	25.35975097735261	27.39932127093979	25.67967856596863
27	22.4339965830559	24.18587460970526	29.884919901608864	23.49520890562998
28	19.990814529129718	25.173839344901666	28.426191805270573	26.409154320698047
29	22.064152120078806	25.6763273691605	28.72652287159905	23.53299763916164
30	26.274595789811663	23.335915350683596	28.381668761962576	22.00782009754217
31	26.36775906107764	24.7297578935845	27.126118940677113	21.776364104660747
32	26.089960803763805	23.964089213327295	27.8651057111096	22.080844271799297
33	26.816213026325407	23.337160080926616	26.811649015434334	23.034977877313644
34	22.239882497465057	23.044488893016712	31.455609887498724	23.260018722019502
35	21.801003380240513	25.55121602165703	30.749145684185127	21.898634913917327
36	24.76927009976034	25.07697379906671	27.76268675342117	22.391069347751777
37	22.49036052175262	26.004840404837342	28.761662563844286	22.74313650956575
38	23.542125660943782	24.625743127892203	28.254738193334695	23.577393017829323
39	23.773262492224426	22.86010923624948	28.03071866575131	25.33590960577478
40	25.20221281121049	21.651284673317356	30.68783474067641	22.458667774795746
41	21.005078180229916	23.324170203775108	29.027715674249627	26.643035941745346
42	21.31456918450062	22.95512364479995	30.604310149753815	25.12599702094562
43	22.127569530152634	22.387526653983183	30.069235726055936	25.415668089808246
44	22.92802682489422	22.56459751009269	28.684872282698027	25.822503382315066
45	22.546086137247787	21.346006602176875	28.423000189262833	27.68490707131251
46	22.980401243581262	22.196316938959388	29.686050308166482	25.137231509292867
47	20.367201804922686	23.367288936039696	32.20480982915599	24.060699429881634
48	20.754408658981895	24.326816372607126	30.13294038157046	24.785834586840522
49	23.461409692107996	21.93367885768233	30.709346232568592	23.89556521764108
50	21.07433624759791	22.868535102509917	29.931677076122277	26.125451573769897
51	20.921745086267784	22.84134253412396	28.33251787544335	27.904394504164898
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1058.0
1	1849.5
2	2641.0
3	4079.5
4	5518.0
5	4236.5
6	2955.0
7	2889.0
8	2823.0
9	3028.5
10	3234.0
11	3588.0
12	3942.0
13	3968.5
14	3995.0
15	4208.0
16	4421.0
17	4256.0
18	4091.0
19	4547.0
20	5003.0
21	6100.0
22	7197.0
23	8494.0
24	9791.0
25	12570.5
26	17551.5
27	19753.0
28	25302.0
29	30851.0
30	35763.5
31	40676.0
32	47580.0
33	54484.0
34	64595.5
35	74707.0
36	81291.0
37	87875.0
38	96008.0
39	104141.0
40	118373.5
41	132606.0
42	145835.0
43	159064.0
44	175524.0
45	191984.0
46	224467.0
47	256950.0
48	288150.5
49	319351.0
50	321238.5
51	323126.0
52	290110.0
53	257094.0
54	234232.0
55	211370.0
56	196428.5
57	181487.0
58	173188.5
59	164890.0
60	147649.5
61	130409.0
62	115429.0
63	100449.0
64	90005.5
65	79562.0
66	65121.0
67	50680.0
68	42317.0
69	33954.0
70	28116.5
71	22279.0
72	18783.5
73	15288.0
74	12112.5
75	7079.0
76	5221.0
77	3585.5
78	1950.0
79	1472.5
80	995.0
81	833.0
82	671.0
83	464.5
84	258.0
85	184.0
86	110.0
87	62.5
88	15.0
89	9.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3133209.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.704781471893583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.73733733446807	15.152566601951426
2	13.552960806225784	6.154340268049902
3	5.716231643061531	3.8935737089530575
4	3.0206955005371	2.7433692493130826
5	1.7940932446704996	2.03672475302221
6	1.1484019737110203	1.5644529512999998
7	0.7819432050141967	1.2427694715295592
8	0.6081326556360929	1.1046015241711842
9	0.4398127419638347	0.8987266975358867
>10	3.9202628227638416	20.454721414847004
>50	1.889691917643269	30.986803438424186
>100	0.3820701765858284	11.246242247531434
>500	0.0055247022673361925	0.7992855687187568
>1k	0.0023677295431440825	1.0204850364854219
>5k	4.735459086288166E-4	0.7013370681668618
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	6637	0.2118275544338089	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	6539	0.20869977074622217	Illumina PCR Primer Index 5 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	6393	0.20404001137491945	TruSeq Adapter, Index 23 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	4442	0.14177158306388116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9149696046449503E-4	0.0	0.0	0.21731713396712443	0.0
2	1.9149696046449503E-4	0.0	0.0	0.795765619210209	0.0
3	1.9149696046449503E-4	0.0	0.0	1.1481200264648799	0.0
4	1.9149696046449503E-4	0.0	0.0	1.5876374668909734	0.0
5	1.9149696046449503E-4	0.0	0.0	2.704766901920683	0.0
6	1.9149696046449503E-4	0.0	0.0	3.5457896361206673	0.0
7	1.9149696046449503E-4	0.0	0.0	4.283052933908973	0.0
8	1.9149696046449503E-4	0.0	0.0	5.536177127028552	0.0
9	1.9149696046449503E-4	0.0	0.0	5.988620612285998	0.0
10	1.9149696046449503E-4	0.0	0.0	6.916965960457793	0.0
11	1.9149696046449503E-4	0.0	0.0	8.381502797930173	0.0
12	1.9149696046449503E-4	0.0	0.0	9.529112165833814	0.0
13	1.9149696046449503E-4	0.0	0.0	9.991481576875337	0.0
14	2.2341312054191087E-4	0.0	0.0	10.196096079131651	0.0
15	2.2341312054191087E-4	0.0	0.0	10.554259227520411	0.0
16	2.553292806193267E-4	0.0	0.0	11.259031874349908	0.0
17	2.553292806193267E-4	0.0	0.0	12.097756645024319	0.0
18	2.553292806193267E-4	0.0	0.0	13.031591572729429	0.0
19	2.553292806193267E-4	0.0	0.0	13.627593946015091	0.0
20	2.553292806193267E-4	0.0	0.0	14.194201535869455	0.0
21	2.553292806193267E-4	0.0	0.0	14.867728261983162	0.0
22	2.553292806193267E-4	0.0	0.0	15.597874256074205	0.0
23	2.553292806193267E-4	0.0	0.0	16.259751583759652	0.0
24	2.553292806193267E-4	0.0	0.0	16.787676787600187	0.0
25	2.553292806193267E-4	0.0	0.0	17.25821673562153	0.0
26	2.553292806193267E-4	0.0	0.0	17.710085729997584	0.0
27	2.553292806193267E-4	0.0	0.0	18.242670693209423	0.0
28	2.553292806193267E-4	0.0	0.0	18.732711415038064	0.0
29	2.553292806193267E-4	0.0	0.0	19.300340322014904	0.0
30	2.553292806193267E-4	0.0	0.0	19.904513232280387	0.0
31	2.553292806193267E-4	0.0	0.0	20.36924443916764	0.0
32	2.553292806193267E-4	0.0	0.0	20.894775931002368	0.0
33	2.553292806193267E-4	0.0	0.0	21.4328823899076	0.0
34	2.553292806193267E-4	0.0	0.0	22.010820216589444	0.0
35	2.8724544069674255E-4	0.0	0.0	22.631366116974643	0.0
36	2.8724544069674255E-4	0.0	0.0	23.19312245049724	0.0
37	2.8724544069674255E-4	0.0	0.0	23.815647152807234	0.0
38	2.8724544069674255E-4	0.0	0.0	24.5289414143774	0.0
39	2.8724544069674255E-4	0.0	0.0	25.773735489716774	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	40	6.8212103E-9	45.0	31
ATACGTC	35	6.251741E-6	38.57143	32
CAATCGA	30	1.14037175E-4	37.499996	30
ACGGGAT	1720	0.0	36.75872	5
CGTAAGG	460	0.0	35.70652	2
TTACGGG	1280	0.0	35.33203	3
TGACGGG	1595	0.0	35.266457	3
GTTAGCG	205	0.0	35.121952	1
TACGGGA	1285	0.0	34.844357	4
TAACGGG	1350	0.0	34.833332	3
TAAGGGA	2865	0.0	34.790577	4
TACGTAG	175	0.0	34.714287	1
ATAACGG	345	0.0	34.56522	2
TCGAACG	85	0.0	34.411766	1
TATGGGA	1875	0.0	34.32	4
AACGAAT	475	0.0	34.105267	24
CGTATGG	390	0.0	34.03846	2
CGTTATT	405	0.0	33.88889	1
GATACGA	60	6.4392225E-9	33.75	9
TCGACGT	60	6.4392225E-9	33.75	26
>>END_MODULE