SRR2935083.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935083.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544383
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	4158	0.7638004860548547	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	3136	0.576065013051473	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	3016	0.5540217089806257	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	2019	0.37087859099200376	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	1966	0.36114279836071295	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	1381	0.25368169101533294	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1177	0.21620807409489273	No Hit
ACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1120	0.20573750466124033	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1007	0.1849800599945259	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC	857	0.15742592990596693	No Hit
TGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCTT	857	0.15742592990596693	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	786	0.14438364166404904	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	674	0.12380989119792499	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	666	0.12234033759320184	No Hit
AAAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	658	0.1208707839884787	No Hit
GGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	653	0.11995231298552672	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT	611	0.11223715656073022	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	600	0.1102165203542359	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	572	0.1050730827377049	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.8030204E-9	45.000004	1
AACGTCC	20	7.0300093E-4	45.000004	21
TACCCGA	20	7.0300093E-4	45.000004	15
ACCGGCC	20	7.0300093E-4	45.000004	34
ACGCCTA	20	7.0300093E-4	45.000004	39
CGTATTA	20	7.0300093E-4	45.000004	26
CATATCG	20	7.0300093E-4	45.000004	24
GTTTCGA	20	7.0300093E-4	45.000004	33
CCCTAAG	20	7.0300093E-4	45.000004	4
CCGGCTA	20	7.0300093E-4	45.000004	21
ATTACGA	20	7.0300093E-4	45.000004	18
GGTCCGT	20	7.0300093E-4	45.000004	8
TCGGGTA	20	7.0300093E-4	45.000004	6
TAAACGC	20	7.0300093E-4	45.000004	44
TGTATCG	20	7.0300093E-4	45.000004	1
TACGGGT	40	6.8030204E-9	45.000004	4
CCGCTAT	20	7.0300093E-4	45.000004	39
ATCGTCG	20	7.0300093E-4	45.000004	1
CATTACG	20	7.0300093E-4	45.000004	17
TTCCCTA	20	7.0300093E-4	45.000004	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content