##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935083.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544383
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87024209058696	33.0	31.0	34.0	30.0	34.0
2	32.181199633346374	34.0	31.0	34.0	30.0	34.0
3	32.2516169682007	34.0	31.0	34.0	30.0	34.0
4	35.81747593146737	37.0	35.0	37.0	35.0	37.0
5	30.29819263276039	37.0	35.0	37.0	0.0	37.0
6	32.78068749391513	37.0	35.0	37.0	17.0	37.0
7	34.96205979981006	37.0	35.0	37.0	32.0	37.0
8	35.4850959710351	37.0	35.0	37.0	33.0	37.0
9	37.313453946945444	39.0	37.0	39.0	34.0	39.0
10	37.31379745510055	39.0	37.0	39.0	34.0	39.0
11	37.21245887546084	39.0	37.0	39.0	34.0	39.0
12	37.03556687111831	39.0	37.0	39.0	33.0	39.0
13	36.896080516842005	39.0	35.0	39.0	33.0	39.0
14	37.9664923408703	40.0	37.0	41.0	33.0	41.0
15	38.094257168206944	40.0	37.0	41.0	33.0	41.0
16	38.220616734909065	40.0	37.0	41.0	34.0	41.0
17	38.19944597829102	40.0	37.0	41.0	34.0	41.0
18	38.14242178760174	40.0	37.0	41.0	34.0	41.0
19	38.11099538376474	40.0	36.0	41.0	34.0	41.0
20	38.00327710453853	40.0	36.0	41.0	34.0	41.0
21	37.947720630511974	40.0	36.0	41.0	34.0	41.0
22	37.9382089448054	40.0	36.0	41.0	34.0	41.0
23	37.833367316760445	40.0	36.0	41.0	33.0	41.0
24	37.810809668927945	40.0	35.0	41.0	33.0	41.0
25	37.75154808287547	39.0	35.0	41.0	33.0	41.0
26	37.70515611251637	39.0	35.0	41.0	33.0	41.0
27	37.655773600571656	39.0	35.0	41.0	33.0	41.0
28	37.623812646610936	39.0	35.0	41.0	33.0	41.0
29	37.59384844861063	39.0	35.0	41.0	33.0	41.0
30	37.47193795544681	39.0	35.0	41.0	33.0	41.0
31	32.89935945832254	38.0	33.0	40.0	9.0	41.0
32	33.67818245610168	38.0	33.0	40.0	16.0	41.0
33	35.62645416921542	38.0	34.0	40.0	28.0	41.0
34	36.50241098638275	38.0	35.0	40.0	31.0	41.0
35	36.56310171331581	39.0	35.0	40.0	31.0	41.0
36	36.75614962260026	39.0	35.0	40.0	31.0	41.0
37	36.83252232343773	39.0	35.0	41.0	32.0	41.0
38	36.76566865607486	39.0	35.0	41.0	31.0	41.0
39	36.83525753008452	39.0	35.0	41.0	32.0	41.0
40	36.794850316780646	39.0	35.0	41.0	31.0	41.0
41	36.685322649678625	39.0	35.0	41.0	31.0	41.0
42	36.59999118267837	39.0	35.0	41.0	31.0	41.0
43	36.61829079894119	39.0	35.0	41.0	31.0	41.0
44	36.58377649559226	39.0	35.0	41.0	31.0	41.0
45	36.56923709961553	39.0	35.0	41.0	31.0	41.0
46	36.51078744192967	39.0	35.0	41.0	31.0	41.0
47	36.380700719897575	39.0	35.0	40.0	31.0	41.0
48	36.3771315415801	39.0	35.0	40.0	31.0	41.0
49	36.40891798605026	39.0	35.0	40.0	31.0	41.0
50	36.23219130648826	39.0	35.0	40.0	31.0	41.0
51	35.625104016841085	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	2.0
12	5.0
13	4.0
14	8.0
15	21.0
16	38.0
17	79.0
18	143.0
19	282.0
20	468.0
21	864.0
22	1426.0
23	2132.0
24	2884.0
25	3471.0
26	3762.0
27	4389.0
28	5185.0
29	6358.0
30	8731.0
31	12252.0
32	19459.0
33	22041.0
34	39197.0
35	59717.0
36	62757.0
37	60795.0
38	95703.0
39	132115.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.854387444134	22.41620329804568	23.704450726786106	11.024958531034217
2	30.062290703420203	26.572835669005094	24.61906415152567	18.745809476049033
3	29.267078509064394	26.289946600095888	27.34618825349065	17.096786637349073
4	27.421319181532123	27.172597233932727	26.51956435083388	18.886519233701275
5	21.313303317700957	39.660863766870015	21.387515774739477	17.63831714068955
6	25.64573103862538	33.40185127015355	24.17948392951286	16.77293376170821
7	73.57779357547903	7.936507936507936	11.876381150770689	6.609317337242346
8	72.2416019603845	8.039744077239737	11.247963290550954	8.4706906718248
9	65.64955187799765	9.08239235979081	14.528006936293014	10.740048825918517
10	38.26368567717949	25.194945470376556	20.059406704470934	16.481962147973025
11	28.287804725717002	25.76256055020087	25.226908261279284	20.722726462802843
12	26.158054164071988	21.011677440331532	31.65400095153596	21.176267444060525
13	23.072542676755152	26.132888058591103	32.554469922830066	18.24009934182368
14	17.368837748423445	32.02910450914154	28.600268560921265	22.00178918151375
15	14.82099918623469	26.406776111671377	38.41358014486125	20.358644557232683
16	16.495922907217896	26.2590859743967	33.281531568766844	23.96345954961856
17	16.626345789637075	26.62133093796096	27.660672724901403	29.091650547500564
18	17.565023154653982	26.739997391542353	31.65565419934127	24.03932525446239
19	19.580148535130597	27.880187294606923	28.31995121082032	24.21971295944216
20	22.17189001126045	28.04220557952765	28.70736227986546	21.078542129346435
21	19.91759478161515	29.129858941223368	30.133564053249273	20.818982223912208
22	18.47063556356462	26.086964508443504	27.54347582492473	27.89892410306714
23	17.672667956199955	28.718751320302065	27.787605417509365	25.820975305988615
24	21.15587738779499	24.34260437963713	29.35139414713538	25.150124085432502
25	17.17008062338464	29.236768965966974	27.555967030564876	26.037183380083505
26	17.06335429284162	28.13644070443052	30.75904280625956	24.041162196468296
27	21.233212646243548	28.700381900243027	27.34949474910128	22.71691070441215
28	16.884436141466576	26.55758905035609	30.17618110778625	26.381793700391086
29	18.39036119790662	25.55186330212369	30.009937856251938	26.047837643717752
30	22.608898514465	26.53370880427934	26.36673077594267	24.490661905312987
31	25.08564742102527	27.990403814961155	21.831688351767045	25.092260412246524
32	21.011677440331532	30.83178570969336	24.29649713528894	23.860039714686167
33	21.66085274521798	26.296008508715374	25.21147794842969	26.831660797636957
34	20.78720312721007	24.516930175997416	27.779890261084567	26.91597643570795
35	20.36654340785807	27.976994138318055	26.67239792572509	24.984064528098784
36	22.191728984924218	24.287496119460013	29.366457071583792	24.154317824031978
37	22.430347751491137	25.965726336053844	29.947114439650026	21.656811472804993
38	18.928401511435883	29.8692280985997	24.192709911955372	27.00966047800905
39	22.19246376172658	25.336022616429975	28.0335719520999	24.437941669743545
40	23.784357704042925	23.330265640183473	29.12269486740034	23.76268178837326
41	20.979898343629394	25.20504865140903	26.87684957098219	26.938203433979385
42	21.276013394981106	22.85192594184609	31.23499447998927	24.63706618318353
43	23.116812979097435	21.990032752675965	30.178201743992737	24.71495252423386
44	20.77305867376461	23.38463912355823	27.343249146281202	28.499053056395958
45	20.51570309873747	23.36626970349919	26.907342808280198	29.210684389483138
46	24.226142256462822	23.769478473795104	27.583521160653433	24.420858109088638
47	19.56453452808041	23.000718244324307	32.8961411359282	24.53860609166708
48	19.17565390543055	22.575466169957547	31.106041151174818	27.142838773437084
49	20.709316786159746	20.38564760471947	34.17079519382494	24.73424041529585
50	20.236120525438892	20.310516676678	32.34965088917178	27.103711908711333
51	19.303137680640283	19.631031828694137	28.10208988892012	32.963740601745464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1262.0
1	1258.5
2	1255.0
3	1324.0
4	1393.0
5	1233.0
6	1073.0
7	1101.0
8	1129.0
9	1258.5
10	1388.0
11	1558.0
12	1728.0
13	1898.0
14	2068.0
15	2024.5
16	1981.0
17	2070.5
18	2160.0
19	2086.0
20	2012.0
21	2126.5
22	2241.0
23	2346.0
24	2451.0
25	2520.5
26	3023.5
27	3457.0
28	4171.0
29	4885.0
30	5617.5
31	6350.0
32	6847.0
33	7344.0
34	8601.0
35	9858.0
36	10508.5
37	11159.0
38	12809.0
39	14459.0
40	17354.5
41	20250.0
42	25700.0
43	31150.0
44	37046.0
45	42942.0
46	55007.0
47	67072.0
48	70904.5
49	74737.0
50	72056.0
51	69375.0
52	56514.0
53	43653.0
54	36669.0
55	29685.0
56	25279.5
57	20874.0
58	17879.5
59	14885.0
60	14251.5
61	13618.0
62	12715.5
63	11813.0
64	10219.0
65	8625.0
66	7054.5
67	5484.0
68	4357.5
69	3231.0
70	2592.5
71	1954.0
72	1677.5
73	1401.0
74	989.0
75	508.5
76	440.0
77	362.5
78	285.0
79	167.5
80	50.0
81	44.0
82	38.0
83	19.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	544383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.800180019861582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.21291812694768	20.625574198829817
2	12.741942491013859	7.59424360069873
3	5.735185270936757	5.127286605635219
4	2.9572555687221835	3.525069932506369
5	1.7512458499936157	2.6093720794422626
6	1.1844822612210726	2.1178670768832406
7	0.785508368429509	1.6385803550414988
8	0.592821529009253	1.413295062730027
9	0.42741149704679404	1.14632455990977
>10	3.5506774760920226	25.07167467009881
>50	0.94810076556145	18.504729270747212
>100	0.09769162817868166	4.979875588497917
>500	0.008433809626936547	1.6709192684121792
>1k	0.0063253572202024095	3.9751877305669536
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	4158	0.7638004860548547	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	3136	0.576065013051473	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	3016	0.5540217089806257	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	2019	0.37087859099200376	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	1966	0.36114279836071295	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	1381	0.25368169101533294	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1177	0.21620807409489273	No Hit
ACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1120	0.20573750466124033	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1007	0.1849800599945259	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC	857	0.15742592990596693	No Hit
TGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCTT	857	0.15742592990596693	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	786	0.14438364166404904	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	674	0.12380989119792499	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	666	0.12234033759320184	No Hit
AAAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	658	0.1208707839884787	No Hit
GGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	653	0.11995231298552672	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT	611	0.11223715656073022	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	600	0.1102165203542359	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	572	0.1050730827377049	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5859844998833542	0.0
2	0.0	0.0	0.0	2.4587468749024124	0.0
3	0.0	0.0	0.0	3.4108339165624204	0.0
4	0.0	0.0	0.0	4.467810346759542	0.0
5	0.0	0.0	0.0	7.452841106353431	0.0
6	1.8369420059039315E-4	0.0	0.0	9.186546971525562	0.0
7	1.8369420059039315E-4	0.0	0.0	10.777706137039548	0.0
8	1.8369420059039315E-4	0.0	0.0	13.538997360314337	0.0
9	1.8369420059039315E-4	0.0	0.0	14.507984268428661	0.0
10	1.8369420059039315E-4	0.0	0.0	16.87984378645182	0.0
11	1.8369420059039315E-4	0.0	0.0	19.741799431650144	0.0
12	1.8369420059039315E-4	0.0	0.0	22.38809808535535	0.0
13	1.8369420059039315E-4	0.0	0.0	23.349553531245466	0.0
14	1.8369420059039315E-4	0.0	0.0	23.746333004520714	0.0
15	1.8369420059039315E-4	0.0	0.0	24.495621648728928	0.0
16	1.8369420059039315E-4	0.0	0.0	25.88324763998876	0.0
17	1.8369420059039315E-4	0.0	0.0	27.54825187414008	0.0
18	1.8369420059039315E-4	0.0	0.0	29.77131908968502	0.0
19	1.8369420059039315E-4	0.0	0.0	31.029256975328032	0.0
20	3.673884011807863E-4	0.0	0.0	31.983180958993945	0.0
21	3.673884011807863E-4	0.0	0.0	33.00488810267771	0.0
22	3.673884011807863E-4	0.0	0.0	33.95513820233182	0.0
23	3.673884011807863E-4	0.0	0.0	34.85670933882946	0.0
24	3.673884011807863E-4	0.0	0.0	35.57256563853023	0.0
25	3.673884011807863E-4	0.0	0.0	36.234599537458	0.0
26	3.673884011807863E-4	0.0	0.0	36.80699066649767	0.0
27	3.673884011807863E-4	0.0	0.0	37.360644987077116	0.0
28	3.673884011807863E-4	0.0	0.0	37.900522242612276	0.0
29	3.673884011807863E-4	0.0	0.0	38.517734756596	0.0
30	3.673884011807863E-4	0.0	0.0	39.199791323388126	0.0
31	3.673884011807863E-4	0.0	0.0	39.768324874215395	0.0
32	3.673884011807863E-4	0.0	0.0	40.40592744446465	0.0
33	3.673884011807863E-4	0.0	0.0	40.98750328353383	0.0
34	3.673884011807863E-4	0.0	0.0	41.577529055830176	0.0
35	3.673884011807863E-4	0.0	0.0	42.20190564363693	0.0
36	3.673884011807863E-4	0.0	0.0	42.772459830670684	0.0
37	3.673884011807863E-4	0.0	0.0	43.342462935102674	0.0
38	3.673884011807863E-4	0.0	0.0	43.94700054924566	0.0
39	3.673884011807863E-4	0.0	0.0	44.57468363266303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.8030204E-9	45.000004	1
AACGTCC	20	7.0300093E-4	45.000004	21
TACCCGA	20	7.0300093E-4	45.000004	15
ACCGGCC	20	7.0300093E-4	45.000004	34
ACGCCTA	20	7.0300093E-4	45.000004	39
CGTATTA	20	7.0300093E-4	45.000004	26
CATATCG	20	7.0300093E-4	45.000004	24
GTTTCGA	20	7.0300093E-4	45.000004	33
CCCTAAG	20	7.0300093E-4	45.000004	4
CCGGCTA	20	7.0300093E-4	45.000004	21
ATTACGA	20	7.0300093E-4	45.000004	18
GGTCCGT	20	7.0300093E-4	45.000004	8
TCGGGTA	20	7.0300093E-4	45.000004	6
TAAACGC	20	7.0300093E-4	45.000004	44
TGTATCG	20	7.0300093E-4	45.000004	1
TACGGGT	40	6.8030204E-9	45.000004	4
CCGCTAT	20	7.0300093E-4	45.000004	39
ATCGTCG	20	7.0300093E-4	45.000004	1
CATTACG	20	7.0300093E-4	45.000004	17
TTCCCTA	20	7.0300093E-4	45.000004	17
>>END_MODULE