##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935082.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2055439
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.868651417045214	33.0	31.0	34.0	30.0	34.0
2	32.201262114808564	34.0	31.0	34.0	30.0	34.0
3	32.25749584395353	34.0	31.0	34.0	30.0	34.0
4	35.8084540577463	37.0	35.0	37.0	35.0	37.0
5	30.301743325878316	37.0	35.0	37.0	0.0	37.0
6	32.77563722397016	37.0	35.0	37.0	17.0	37.0
7	35.0284182600408	37.0	35.0	37.0	32.0	37.0
8	35.62369547332711	37.0	35.0	37.0	35.0	37.0
9	37.52379029491997	39.0	37.0	39.0	35.0	39.0
10	37.25972213235226	39.0	37.0	39.0	34.0	39.0
11	37.18071954458391	39.0	37.0	39.0	34.0	39.0
12	37.176889219286004	39.0	37.0	39.0	34.0	39.0
13	37.20886827582818	39.0	37.0	39.0	34.0	39.0
14	38.386383152212254	40.0	38.0	41.0	34.0	41.0
15	38.46998037888743	40.0	38.0	41.0	34.0	41.0
16	38.5207836379479	40.0	38.0	41.0	34.0	41.0
17	38.52051654172175	40.0	38.0	41.0	34.0	41.0
18	38.472270400629746	40.0	38.0	41.0	34.0	41.0
19	38.450763559512104	40.0	38.0	41.0	34.0	41.0
20	38.39223251091373	40.0	38.0	41.0	34.0	41.0
21	38.34830904736166	40.0	38.0	41.0	34.0	41.0
22	38.34854160108863	40.0	37.0	41.0	34.0	41.0
23	38.279441520765154	40.0	37.0	41.0	34.0	41.0
24	38.27150890880245	40.0	37.0	41.0	34.0	41.0
25	38.22653943999311	40.0	37.0	41.0	34.0	41.0
26	38.172019213413776	40.0	37.0	41.0	34.0	41.0
27	38.15327869131607	40.0	37.0	41.0	34.0	41.0
28	38.13628329519874	40.0	37.0	41.0	34.0	41.0
29	38.14407968322096	40.0	37.0	41.0	34.0	41.0
30	38.0149121428561	40.0	37.0	41.0	34.0	41.0
31	33.39665930246531	39.0	34.0	41.0	9.0	41.0
32	34.32411129690543	39.0	34.0	41.0	16.0	41.0
33	36.28003798701883	39.0	34.0	41.0	30.0	41.0
34	37.13966359497898	39.0	35.0	41.0	32.0	41.0
35	37.31678147587936	39.0	36.0	41.0	33.0	41.0
36	37.48891696615662	39.0	36.0	41.0	33.0	41.0
37	37.58969057218434	40.0	37.0	41.0	33.0	41.0
38	37.60398143656902	40.0	37.0	41.0	33.0	41.0
39	37.60069016886417	40.0	37.0	41.0	33.0	41.0
40	37.595921844433235	40.0	37.0	41.0	33.0	41.0
41	37.54343086805301	40.0	36.0	41.0	33.0	41.0
42	37.49171442207723	40.0	36.0	41.0	33.0	41.0
43	37.52145502736885	40.0	36.0	41.0	33.0	41.0
44	37.476956990696394	40.0	36.0	41.0	33.0	41.0
45	37.471775129303275	40.0	36.0	41.0	33.0	41.0
46	37.42616978660033	40.0	36.0	41.0	33.0	41.0
47	37.33123970110521	39.0	36.0	41.0	33.0	41.0
48	37.35141835880316	39.0	36.0	41.0	33.0	41.0
49	37.35780190995695	39.0	36.0	41.0	33.0	41.0
50	37.19442124042601	39.0	36.0	41.0	32.0	41.0
51	36.57383945716706	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	10.0
11	12.0
12	15.0
13	20.0
14	31.0
15	42.0
16	88.0
17	138.0
18	315.0
19	528.0
20	913.0
21	1625.0
22	2592.0
23	4181.0
24	6214.0
25	8261.0
26	10062.0
27	12537.0
28	15768.0
29	20331.0
30	27537.0
31	38708.0
32	57336.0
33	72889.0
34	120396.0
35	178808.0
36	249520.0
37	241171.0
38	393393.0
39	591600.0
40	391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.22371863139699	22.08963632586518	26.594707991820727	11.091937050917103
2	32.63259089664057	23.559979157737107	25.676218073122094	18.13121187250023
3	31.782164296775527	23.610382015715377	27.761271436418205	16.846182251090884
4	27.637404953394384	26.625407029836452	27.180470935892526	18.556717080876638
5	21.602051921754914	39.3675998168761	22.115956737222557	16.914391524146424
6	25.578477395826386	33.56733038538239	25.05600993267132	15.7981822861199
7	79.19398240473203	5.65747755102438	9.447714089301604	5.700825954941986
8	79.18790097881767	4.539224953890629	9.395608432067311	6.877265635224397
9	73.569928370533	6.855177896303418	12.161051726662771	7.413842006500801
10	35.70964645508818	26.160640135756886	22.081706146472847	16.048007262682084
11	25.097217674667068	26.532385539050296	27.274562757639607	21.09583402864303
12	21.1462368866213	23.068453989634328	33.134089603242906	22.65121952050146
13	22.39993500171983	24.41624392647994	34.58862072773748	18.595200344062754
14	19.918956485694782	28.123529815285202	30.743651356230956	21.213862342789056
15	18.389064331269378	27.625679964231487	33.9442814892585	20.040974215240638
16	20.007793955451852	28.43207704047651	30.1730189998341	21.387110004237538
17	19.971791914038803	27.579947641355446	28.43806116357625	24.010199281029504
18	20.301356547190164	26.71604460166417	31.694835020645222	21.28776383050044
19	19.796695499112353	28.916061240445472	29.701440908730447	21.585802351711727
20	20.5796912484389	29.173135276697582	29.761817305208275	20.48535616965524
21	20.071770556070987	29.06717251156565	31.002817402997607	19.858239529365747
22	19.054956143188875	26.940960057681107	28.548207949737257	25.455875849392758
23	18.311903199267892	28.207842704161983	29.3559672653871	24.124286831183024
24	20.583583361024093	26.32693064595933	29.491072223500677	23.598413769515904
25	18.74952260806572	28.370289753186544	27.610889936407744	25.269297702339987
26	17.670045182561974	28.024426898584682	29.92494547393525	24.38058244491809
27	19.798641555404952	27.14203632411373	30.33308212990023	22.72623999058109
28	17.650244059784796	25.946427989349235	31.59393200187405	24.80939594899192
29	20.144212501562926	23.965829197558282	29.63313433286028	26.256823968018512
30	21.942027956071673	24.90032542926353	30.122713444670456	23.034933169994343
31	22.279620071429996	27.375709033447354	27.755335964725784	22.589334930396866
32	21.01361315028079	26.096177021064598	27.85687145179205	25.033338376862556
33	21.79704676227317	25.556827519571247	29.34103128334142	23.30509443481417
34	19.50809535092017	23.94753626840787	31.513900436840984	25.030467943830974
35	20.19729118694352	23.81106907088948	28.72670996317575	27.264929778991252
36	20.9048286035246	25.868245177794137	30.4154003110771	22.811525907604167
37	18.75827986138241	25.466871067445933	34.14832549153733	21.626523579634327
38	21.327901241535262	26.117437686061223	28.57019838584361	23.9844626865599
39	24.065661885368527	24.784097217188155	29.152409777181422	21.9978311202619
40	22.29080989511243	22.807633795018972	30.795465105021357	24.10609120484724
41	17.972219073395028	24.564679370197805	29.25851849653529	28.20458305987188
42	19.54969230417444	24.519141652951024	30.304815662250256	25.626350380624284
43	21.3853585535742	23.716150175218043	31.13388429430404	23.764606976903718
44	20.96141019023187	23.517068616485336	29.56005991907325	25.961461274209547
45	22.222406016427634	21.10814283469371	29.553978493158883	27.115472655719774
46	23.691581214524003	22.07294889315616	30.35570503430167	23.879764858018167
47	18.48563737478952	23.25994592882591	33.284179194809475	24.97023750157509
48	18.742662759634314	22.34632115085877	32.30365873178431	26.60735735772261
49	21.684272800117153	20.918596951794726	33.319256859483545	24.077873388604576
50	20.926381176965116	20.116967713466565	32.61288707667802	26.3437640328903
51	19.862277596172888	19.59566788408705	29.670352659456206	30.871701860283864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1427.0
1	1688.0
2	1949.0
3	2492.0
4	3035.0
5	2672.5
6	2310.0
7	2441.5
8	2573.0
9	2824.0
10	3075.0
11	3411.5
12	3748.0
13	3982.0
14	4216.0
15	4226.0
16	4236.0
17	4504.0
18	4772.0
19	4839.0
20	4906.0
21	5578.0
22	6250.0
23	6817.0
24	7384.0
25	8982.0
26	13030.0
27	15480.0
28	18323.5
29	21167.0
30	25215.0
31	29263.0
32	33122.0
33	36981.0
34	43766.0
35	50551.0
36	55301.5
37	60052.0
38	67630.5
39	75209.0
40	83726.5
41	92244.0
42	108077.5
43	123911.0
44	145090.5
45	166270.0
46	201274.5
47	236279.0
48	267969.5
49	299660.0
50	285660.5
51	271661.0
52	226363.5
53	181066.0
54	149941.0
55	118816.0
56	96667.0
57	74518.0
58	62504.0
59	50490.0
60	43593.0
61	36696.0
62	29309.5
63	21923.0
64	17922.5
65	13922.0
66	11103.5
67	8285.0
68	6639.0
69	4993.0
70	3878.5
71	2764.0
72	2054.5
73	1345.0
74	1000.5
75	536.5
76	417.0
77	316.5
78	216.0
79	159.5
80	103.0
81	63.5
82	24.0
83	19.5
84	15.0
85	7.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2055439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.16497297507067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.81989381397032	17.693206115672833
2	14.946271328954753	8.419226561161684
3	6.876731951510477	5.810489087132926
4	3.8002184360693523	4.281321982050345
5	2.295733473714524	3.232963562256734
6	1.4600703933755939	2.467370590267464
7	1.0329571061946807	2.0365246286266268
8	0.7422393838340884	1.6724121749376164
9	0.5691724385498307	1.4427653714919948
>10	4.607894923952687	29.813223844951242
>50	0.7484457413122272	13.599555605054306
>100	0.08799138592607823	4.416516154934045
>500	0.008380052245517649	1.5820041333336377
>1k	0.0034282031913481286	1.8678613158419435
>5k	3.8091146570534766E-4	1.0527740784214692
>10k+	1.9045573285267383E-4	0.6117847938651206
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	11405	0.5548693004268188	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	9913	0.4822814007129377	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	9713	0.472551119249951	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	4402	0.21416349500033816	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCC	3516	0.17105834811930687	No Hit
ACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	3070	0.14935982045684645	No Hit
TGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCTT	2305	0.11214149386092216	Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	2212	0.10761691298063333	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTG	2082	0.10129223002969195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4325703657466848E-4	0.0	0.0	0.5809464547476233	0.0
2	2.4325703657466848E-4	0.0	0.0	1.9652735984867467	0.0
3	2.4325703657466848E-4	0.0	0.0	2.7834929667093014	0.0
4	2.4325703657466848E-4	0.0	0.0	3.7710192323878258	0.0
5	2.4325703657466848E-4	0.0	0.0	6.053451355160625	0.0
6	2.4325703657466848E-4	0.0	0.0	7.656466574780375	0.0
7	2.4325703657466848E-4	0.0	0.0	9.015689592344993	0.0
8	2.4325703657466848E-4	0.0	0.0	11.402576286622955	0.0
9	2.4325703657466848E-4	0.0	0.0	12.185231476098293	0.0
10	2.4325703657466848E-4	0.0	0.0	13.938044378840724	0.0
11	2.4325703657466848E-4	0.0	0.0	16.7162343421527	0.0
12	2.4325703657466848E-4	0.0	0.0	18.89970950244692	0.0
13	2.4325703657466848E-4	0.0	0.0	19.77704033055712	0.0
14	2.9190844388960217E-4	0.0	0.0	20.10402643912079	0.0
15	3.8921125851946954E-4	0.0	0.0	20.70662277012356	0.0
16	3.8921125851946954E-4	0.0	0.0	22.053974844303333	0.0
17	3.8921125851946954E-4	0.0	0.0	23.695424675701883	0.0
18	3.8921125851946954E-4	0.0	0.0	25.52758802377497	0.0
19	3.8921125851946954E-4	0.0	0.0	26.67293945478314	0.0
20	3.8921125851946954E-4	0.0	0.0	27.668931065334462	0.0
21	3.8921125851946954E-4	0.0	0.0	28.792973179938688	0.0
22	3.8921125851946954E-4	0.0	0.0	29.98410558523021	0.0
23	3.8921125851946954E-4	0.0	0.0	31.16575096609532	1.9460562925973477E-4
24	3.8921125851946954E-4	0.0	0.0	32.069742765414105	1.9460562925973477E-4
25	3.8921125851946954E-4	0.0	0.0	32.890492006817034	1.9460562925973477E-4
26	3.8921125851946954E-4	0.0	0.0	33.64507533427166	1.9460562925973477E-4
27	4.3786266583440325E-4	0.0	0.0	34.37411667288594	1.9460562925973477E-4
28	4.3786266583440325E-4	0.0	0.0	35.137700510693826	1.9460562925973477E-4
29	4.8651407314933697E-4	0.0	0.0	35.90610083782588	1.9460562925973477E-4
30	4.8651407314933697E-4	0.0	0.0	36.695178013066794	1.9460562925973477E-4
31	4.8651407314933697E-4	0.0	0.0	37.45112357992623	1.9460562925973477E-4
32	4.8651407314933697E-4	0.0	0.0	38.30918845073972	1.9460562925973477E-4
33	4.8651407314933697E-4	0.0	0.0	39.14594400514927	1.9460562925973477E-4
34	5.351654804642707E-4	0.0	0.0	39.92675044114664	1.9460562925973477E-4
35	5.351654804642707E-4	0.0	0.0	40.718503443789864	1.9460562925973477E-4
36	5.351654804642707E-4	0.0	0.0	41.480627739378306	1.9460562925973477E-4
37	5.351654804642707E-4	0.0	0.0	42.255839263534455	1.9460562925973477E-4
38	5.351654804642707E-4	0.0	0.0	43.03528346012701	1.9460562925973477E-4
39	5.351654804642707E-4	0.0	0.0	43.80796511110279	1.9460562925973477E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGA	20	7.0345844E-4	45.000004	11
TTAGCCG	30	2.16634E-6	45.000004	1
CCGTTGT	20	7.0345844E-4	45.000004	29
TACCGGA	20	7.0345844E-4	45.000004	3
GGCGTAA	45	3.8562575E-10	45.0	8
GTACGAC	25	3.8918835E-5	45.0	9
TAGTACG	80	0.0	42.187504	1
CTAACGG	165	0.0	40.909092	2
CGGTTGT	240	0.0	40.312504	11
ACGCCTA	365	0.0	39.452053	25
TAGAACG	80	0.0	39.375004	1
TCATGCG	80	0.0	39.375004	1
TACACGG	120	0.0	39.375004	2
TCGCGCG	40	3.4599907E-7	39.375004	1
GCGCGAG	110	0.0	38.863636	1
TATACGC	255	0.0	38.82353	26
AACGTCG	35	6.250695E-6	38.571426	1
TTATCCG	35	6.250695E-6	38.571426	19
ATACGCA	275	0.0	38.454544	27
CTAACGC	205	0.0	38.414635	29
>>END_MODULE