SRR2935080.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935080.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577392
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	5744	0.9948180785324355	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	5595	0.9690123867320642	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	4854	0.840676697979882	No Hit
TCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	2117	0.3666486546401751	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1868	0.3235237065979439	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	1293	0.22393798320724914	No Hit
AAAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	1235	0.2138928145869704	No Hit
ACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	1225	0.21216088896278437	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	1163	0.2014229500928312	No Hit
TGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCTT	1133	0.19622717322027322	Illumina Single End Adapter 1 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	1041	0.1802934574777621	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	1014	0.1756172582924599	No Hit
AGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	878	0.15206306980353038	No Hit
GAAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	782	0.1354365838113448	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	741	0.12833568875218224	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	738	0.12781611106492644	No Hit
GGCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	699	0.12106160113060105	No Hit
GACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	658	0.11396070607143846	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	631	0.10928450688613628	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT	611	0.10582065563776429	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	45	3.8380676E-10	45.000004	1
AACGTCA	20	7.030365E-4	45.000004	13
GCGACAG	20	7.030365E-4	45.000004	1
AATTTAC	20	7.030365E-4	45.000004	40
TTAGCCG	20	7.030365E-4	45.000004	1
GCGCGAG	20	7.030365E-4	45.000004	1
GACGTCA	20	7.030365E-4	45.000004	3
GAACTAC	20	7.030365E-4	45.000004	12
ATCAGCC	20	7.030365E-4	45.000004	22
AGGCGAA	20	7.030365E-4	45.000004	14
TGCGGCA	20	7.030365E-4	45.000004	24
GCGCATT	40	6.8048394E-9	45.000004	25
TAGCCGA	20	7.030365E-4	45.000004	5
TCCTACG	20	7.030365E-4	45.000004	27
ACCTCGG	20	7.030365E-4	45.000004	20
GCCGATA	20	7.030365E-4	45.000004	9
GTAGCCC	20	7.030365E-4	45.000004	21
GCCGAGT	20	7.030365E-4	45.000004	18
CGCTATT	20	7.030365E-4	45.000004	39
GAGCGAA	20	7.030365E-4	45.000004	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content