##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935080.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577392
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.769331753817163	33.0	31.0	34.0	30.0	34.0
2	32.07848913736248	33.0	31.0	34.0	30.0	34.0
3	32.148141990190375	34.0	31.0	34.0	30.0	34.0
4	35.74127629063098	37.0	35.0	37.0	35.0	37.0
5	30.200033945742234	37.0	35.0	37.0	0.0	37.0
6	32.699639066699916	37.0	35.0	37.0	17.0	37.0
7	34.89815757752099	37.0	35.0	37.0	32.0	37.0
8	35.428403926621776	37.0	35.0	37.0	33.0	37.0
9	37.19790887300136	39.0	37.0	39.0	34.0	39.0
10	37.246551736082246	39.0	37.0	39.0	34.0	39.0
11	37.1757419569374	39.0	37.0	39.0	34.0	39.0
12	37.05141221215396	39.0	37.0	39.0	33.0	39.0
13	36.93705316318896	39.0	37.0	39.0	33.0	39.0
14	37.98910445589824	40.0	37.0	41.0	33.0	41.0
15	38.12504156621498	40.0	37.0	41.0	33.0	41.0
16	38.19727498822291	40.0	37.0	41.0	34.0	41.0
17	38.198717682267855	40.0	37.0	41.0	34.0	41.0
18	38.12112741430432	40.0	37.0	41.0	34.0	41.0
19	38.06082003214454	40.0	36.0	41.0	34.0	41.0
20	37.98101463130767	40.0	36.0	41.0	34.0	41.0
21	37.888155707041314	39.0	36.0	41.0	33.0	41.0
22	37.8527655388367	39.0	36.0	41.0	33.0	41.0
23	37.77685004295176	39.0	35.0	41.0	33.0	41.0
24	37.77034666223294	39.0	35.0	41.0	33.0	41.0
25	37.67723314489982	39.0	35.0	41.0	33.0	41.0
26	37.53909475711475	39.0	35.0	41.0	33.0	41.0
27	37.46584123091418	39.0	35.0	41.0	33.0	41.0
28	37.40822699310001	39.0	35.0	41.0	33.0	41.0
29	37.264553371020035	39.0	35.0	41.0	33.0	41.0
30	37.02692451575359	39.0	35.0	40.0	32.0	41.0
31	32.46411623299249	38.0	32.0	40.0	9.0	41.0
32	33.19893936874775	38.0	31.0	40.0	16.0	41.0
33	34.94363101670962	38.0	33.0	40.0	25.0	41.0
34	35.800851068251724	38.0	35.0	40.0	30.0	41.0
35	35.9157522099371	38.0	35.0	40.0	30.0	41.0
36	36.0431942250672	38.0	35.0	40.0	30.0	41.0
37	36.12087801701444	38.0	35.0	40.0	30.0	41.0
38	36.07472046720426	38.0	35.0	40.0	30.0	41.0
39	36.100145135367306	38.0	35.0	40.0	30.0	41.0
40	36.03711689805193	38.0	35.0	40.0	30.0	41.0
41	36.024794247235846	39.0	35.0	40.0	30.0	41.0
42	35.95314967994015	39.0	35.0	40.0	30.0	41.0
43	35.89737128328761	39.0	35.0	40.0	30.0	41.0
44	35.798611688419655	38.0	35.0	40.0	30.0	41.0
45	35.79612117937207	38.0	35.0	40.0	29.0	41.0
46	35.73357788123147	38.0	35.0	40.0	29.0	41.0
47	35.63996383739297	38.0	35.0	40.0	28.0	41.0
48	35.682962354864635	38.0	35.0	40.0	28.0	41.0
49	35.716618865519436	38.0	35.0	40.0	28.0	41.0
50	35.51252009033724	38.0	35.0	40.0	28.0	41.0
51	34.904615928173584	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	10.0
13	7.0
14	10.0
15	25.0
16	35.0
17	121.0
18	224.0
19	455.0
20	816.0
21	1394.0
22	2215.0
23	3574.0
24	4583.0
25	5047.0
26	5301.0
27	5745.0
28	6600.0
29	7936.0
30	9934.0
31	14019.0
32	20929.0
33	24706.0
34	42034.0
35	62419.0
36	67086.0
37	69554.0
38	110368.0
39	112182.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67497990966276	23.096960124144427	23.765137029955387	11.462922936237426
2	30.59689084711946	27.298611688419655	23.284874054368608	18.819623410092277
3	28.75048493917477	27.150185662426914	26.47560063180647	17.62372876659185
4	26.841556516196967	28.83863995344584	25.253900296505666	19.065903233851525
5	20.07093967356666	40.632880261590046	21.7548909579627	17.541289106880594
6	23.859353783911104	33.429628398038076	25.902852827888157	16.808164990162663
7	71.49908554327044	9.027835508631918	12.35521101781805	7.117867930279602
8	68.14486518690941	8.406420594673984	13.113794441211516	10.334919777205087
9	61.81814088175798	10.753006622883587	17.06206528666833	10.366787208690111
10	34.321743287056286	27.736096101089036	22.052608972760275	15.88955163909441
11	26.174245573198107	27.19365699559398	26.36163992573503	20.270457505472887
12	24.412877213400947	23.041365311608057	32.8572269792446	19.688530495746388
13	21.305109873361598	28.915364258597283	32.12444924765151	17.655076620389615
14	15.394047718014797	34.22492864466428	29.224686175076897	21.15633746224402
15	13.653289273145456	29.67481364480284	37.34793693019647	19.32396015185524
16	14.511112034804777	31.75797378557375	31.1587275196054	22.572186660016072
17	14.920019674675093	29.35302186382908	26.666458835591765	29.06049962590406
18	16.295861390528447	28.809716795521933	31.872107684207606	23.022314129742014
19	17.612817635159477	30.40759137639593	28.770748468977747	23.20884251946684
20	19.937408207941917	30.595678499182533	28.740093385429656	20.726819907445897
21	18.486400919998893	31.627040208385292	30.60104746861751	19.28551140299831
22	16.336042065009558	29.692479286169537	25.896444703078668	28.075033945742234
23	16.814573114972152	30.245829523096962	27.60931914539862	25.33027821653227
24	20.156323606839027	27.272459611494444	27.976487377726052	24.594729403940477
25	16.2246792473744	31.434796464100646	25.397476930750685	26.94304735777427
26	15.425222379250144	34.55780474963283	27.01111203480478	23.005860836312245
27	20.53457616316125	33.86503449995843	25.374788705073847	20.22560063180647
28	17.174986837365257	31.449344639343806	27.713754260537037	23.6619142627539
29	16.52655388367002	32.851165239559954	27.0327611051071	23.589519771662925
30	19.260571674010034	35.90056668606423	23.19689223265996	21.641969407265773
31	23.885679053398732	33.008250893673626	19.75798071327625	23.3480893396514
32	20.471880455565717	32.389780253276804	22.424799789397845	24.713539501759637
33	20.863988416881426	33.1672416659739	22.589332723695513	23.379437193449164
34	19.365526366835702	26.951880178457614	25.422243467176546	28.260349987530137
35	17.34281043034888	28.496757835231524	24.227734364175465	29.93269737024413
36	23.9859575470391	31.03576080028819	23.49980602433009	21.478475628342615
37	22.61236733449719	26.9787250256325	28.009393964585584	22.39951367528473
38	21.128626652257047	27.858716435281405	25.885013993959046	25.127642918502506
39	24.428984121705877	25.303433369357386	26.599433313935766	23.66814919500097
40	22.152194696150968	24.710248843073686	27.393521212625043	25.744035248150304
41	19.10833541164408	26.17095491451215	22.958059689084713	31.762649984759054
42	24.893313381550144	23.824368886302548	26.059938482001826	25.222379250145483
43	25.739532241527417	23.59991132540804	27.906690775071358	22.753865657993185
44	21.75887438689833	24.22323135755258	27.08593122176961	26.93196303377948
45	20.853770055698728	22.499272591237844	27.10844625488403	29.5385110981794
46	23.098692049768616	24.263758417158535	28.187262726189488	24.450286806883366
47	18.148848615845043	22.585349294759887	32.05101560118602	27.214786488209054
48	18.437733809959266	20.532671044974645	31.396520907806135	29.633074237259954
49	22.548112893839885	18.07091196275667	34.23774489428326	25.14323024912018
50	20.25504336741763	18.82533876465209	32.66688835314656	28.25272951478372
51	17.662004322886357	19.102273671959434	27.867202870839915	35.3685191343143
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2354.0
1	2229.0
2	2104.0
3	2031.5
4	1959.0
5	1785.0
6	1611.0
7	1830.5
8	2050.0
9	2291.5
10	2533.0
11	2801.0
12	3069.0
13	3286.5
14	3504.0
15	3592.0
16	3680.0
17	3789.5
18	3899.0
19	3655.5
20	3412.0
21	3057.0
22	2702.0
23	2753.0
24	2804.0
25	2867.5
26	3279.5
27	3628.0
28	3955.5
29	4283.0
30	4910.5
31	5538.0
32	6548.5
33	7559.0
34	8199.0
35	8839.0
36	9485.0
37	10131.0
38	11767.0
39	13403.0
40	16763.0
41	20123.0
42	25656.0
43	31189.0
44	38379.0
45	45569.0
46	57491.0
47	69413.0
48	81751.0
49	94089.0
50	88580.5
51	83072.0
52	65759.5
53	48447.0
54	39286.5
55	30126.0
56	24060.0
57	17994.0
58	15651.0
59	13308.0
60	11894.5
61	10481.0
62	9399.5
63	8318.0
64	6695.0
65	5072.0
66	4050.0
67	3028.0
68	2632.0
69	2236.0
70	1670.5
71	1105.0
72	940.5
73	776.0
74	607.0
75	372.0
76	306.0
77	249.0
78	192.0
79	127.0
80	62.0
81	43.5
82	25.0
83	15.0
84	5.0
85	3.5
86	2.0
87	12.0
88	22.0
89	11.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	577392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.846901836826643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4476529560304	21.798456846518775
2	13.049408879095479	8.311664872023353
3	5.849293580610723	5.5884563542946974
4	3.1023261647976916	3.951979073445239
5	1.9422985338030292	3.092809537191869
6	1.2149329243157692	2.3215109747407823
7	0.8277295882895747	1.8452436061986512
8	0.6147047162262493	1.5661152605033393
9	0.45968714786541315	1.3175650326348562
>10	3.9295408625362334	28.36361688185405
>50	0.4575867189151917	9.218521555272451
>100	0.09065397261527383	5.626601560292309
>500	0.006783630603864029	1.4429348899950294
>1k	0.0061669369126036615	3.3275685097571506
>5k	0.0012333873825207324	2.2269550452774793
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	5744	0.9948180785324355	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	5595	0.9690123867320642	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	4854	0.840676697979882	No Hit
TCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	2117	0.3666486546401751	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1868	0.3235237065979439	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	1293	0.22393798320724914	No Hit
AAAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	1235	0.2138928145869704	No Hit
ACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	1225	0.21216088896278437	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	1163	0.2014229500928312	No Hit
TGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCTT	1133	0.19622717322027322	Illumina Single End Adapter 1 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	1041	0.1802934574777621	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	1014	0.1756172582924599	No Hit
AGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	878	0.15206306980353038	No Hit
GAAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	782	0.1354365838113448	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	741	0.12833568875218224	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTG	738	0.12781611106492644	No Hit
GGCCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	699	0.12106160113060105	No Hit
GACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	658	0.11396070607143846	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	631	0.10928450688613628	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT	611	0.10582065563776429	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0450439216338294	0.0
2	0.0	0.0	0.0	3.567593593260731	0.0
3	0.0	0.0	0.0	4.905159752819575	0.0
4	0.0	0.0	0.0	6.534208994928922	0.0
5	0.0	0.0	0.0	10.346004101199878	0.0
6	0.0	0.0	0.0	12.882582370382686	0.0
7	0.0	0.0	0.0	14.839658325712861	0.0
8	0.0	0.0	0.0	18.138457062099924	0.0
9	0.0	0.0	0.0	19.346994762656912	0.0
10	0.0	0.0	0.0	21.785546041510795	0.0
11	0.0	0.0	0.0	25.598033918031422	0.0
12	0.0	0.0	0.0	28.46731509962036	0.0
13	0.0	0.0	0.0	29.592200792529166	0.0
14	0.0	0.0	0.0	30.03193670850999	0.0
15	0.0	0.0	0.0	30.841611937816943	0.0
16	0.0	0.0	0.0	32.52781472552443	0.0
17	0.0	0.0	0.0	34.47969490398204	0.0
18	0.002424695873860393	0.0	0.0	37.17110732396708	0.0
19	0.002424695873860393	0.0	0.0	38.46485576523402	0.0
20	0.002424695873860393	0.0	0.0	39.566187269653895	0.0
21	0.002424695873860393	0.0	0.0	40.67825671294372	0.0
22	0.002424695873860393	0.0	0.0	41.669610940227784	0.0
23	0.002424695873860393	0.0	0.0	42.60104054091501	0.0
24	0.002424695873860393	0.0	0.0	43.28220688890736	0.0
25	0.002424695873860393	0.0	0.0	43.924924488042784	0.0
26	0.002424695873860393	0.0	0.0	44.53231080444481	0.0
27	0.002424695873860393	0.0	0.0	45.06643666694377	0.0
28	0.002424695873860393	0.0	0.0	45.60697065425222	0.0
29	0.002424695873860393	0.0	0.0	46.17019286723751	0.0
30	0.002424695873860393	0.0	0.0	46.83300080361349	0.0
31	0.002424695873860393	0.0	0.0	47.40869288109291	0.0
32	0.002424695873860393	0.0	0.0	48.02750990661457	0.0
33	0.002424695873860393	0.0	0.0	48.63143237176823	0.0
34	0.002424695873860393	0.0	0.0	49.198811206251555	0.0
35	0.002424695873860393	0.0	0.0	49.82039931277191	0.0
36	0.002424695873860393	0.0	0.0	50.425880510987334	0.0
37	0.002424695873860393	0.0	0.0	51.01248371989913	0.0
38	0.0025978884362789926	0.0	0.0	51.5736276221354	0.0
39	0.0025978884362789926	0.0	0.0	52.18343863441128	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	45	3.8380676E-10	45.000004	1
AACGTCA	20	7.030365E-4	45.000004	13
GCGACAG	20	7.030365E-4	45.000004	1
AATTTAC	20	7.030365E-4	45.000004	40
TTAGCCG	20	7.030365E-4	45.000004	1
GCGCGAG	20	7.030365E-4	45.000004	1
GACGTCA	20	7.030365E-4	45.000004	3
GAACTAC	20	7.030365E-4	45.000004	12
ATCAGCC	20	7.030365E-4	45.000004	22
AGGCGAA	20	7.030365E-4	45.000004	14
TGCGGCA	20	7.030365E-4	45.000004	24
GCGCATT	40	6.8048394E-9	45.000004	25
TAGCCGA	20	7.030365E-4	45.000004	5
TCCTACG	20	7.030365E-4	45.000004	27
ACCTCGG	20	7.030365E-4	45.000004	20
GCCGATA	20	7.030365E-4	45.000004	9
GTAGCCC	20	7.030365E-4	45.000004	21
GCCGAGT	20	7.030365E-4	45.000004	18
CGCTATT	20	7.030365E-4	45.000004	39
GAGCGAA	20	7.030365E-4	45.000004	23
>>END_MODULE