FastQCFastQC Report
Sat 14 Jan 2017
SRR2935079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910575
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT68600.35905421142849664No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC61460.3216832628920613No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC59840.3132041401148869No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC26920.1408999908404538No Hit
TCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC24310.1272391819216728No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA253.89178E-545.036
CGCTATA207.034461E-445.031
TATTACG351.212502E-745.01
ATCGAGT253.89178E-545.028
AACGTAC302.16626E-644.99999633
TATAGCG850.042.352941
GTTTCGC2650.041.6037731
ACGTAAG650.041.538461
CGTTAGG3250.039.4615362
ACGGTTT2800.039.37528
GCTACGA1800.038.75000410
CGATTAC356.250464E-638.5714269
CTATTCG356.250464E-638.57142616
TAGTACG700.038.5714261
AACGCTT2150.037.6744231
CACCGTA301.1402051E-437.49999614
TACGTCA301.1402051E-437.49999619
ACGCATC2350.037.34042729
TTACGCA3100.037.0161329
CGTAAGG2250.037.0000042
Produced by FastQC (version 0.11.5)