##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935079.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1910575
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.071373277678184	33.0	31.0	34.0	30.0	34.0
2	32.39712547269801	34.0	31.0	34.0	30.0	34.0
3	32.45136621174254	34.0	31.0	34.0	30.0	34.0
4	35.957538960784056	37.0	35.0	37.0	35.0	37.0
5	30.380097614592465	37.0	35.0	37.0	0.0	37.0
6	32.88502361854415	37.0	35.0	37.0	17.0	37.0
7	35.11972887743219	37.0	35.0	37.0	32.0	37.0
8	35.693777527707624	37.0	35.0	37.0	35.0	37.0
9	37.60269500019628	39.0	37.0	39.0	35.0	39.0
10	37.47183805922301	39.0	37.0	39.0	35.0	39.0
11	37.35856378315429	39.0	37.0	39.0	35.0	39.0
12	37.25529016133886	39.0	37.0	39.0	34.0	39.0
13	37.25771560917525	39.0	37.0	39.0	34.0	39.0
14	38.49295840257514	40.0	38.0	41.0	34.0	41.0
15	38.5745307041074	40.0	38.0	41.0	35.0	41.0
16	38.63389398479515	40.0	38.0	41.0	35.0	41.0
17	38.609045444434265	40.0	38.0	41.0	35.0	41.0
18	38.56325242400848	40.0	38.0	41.0	35.0	41.0
19	38.57757795428078	40.0	38.0	41.0	34.0	41.0
20	38.49504625570836	40.0	38.0	41.0	34.0	41.0
21	38.454567865694884	40.0	38.0	41.0	34.0	41.0
22	38.45439409601821	40.0	38.0	41.0	34.0	41.0
23	38.38858511181189	40.0	37.0	41.0	34.0	41.0
24	38.38925140337333	40.0	37.0	41.0	34.0	41.0
25	38.34207136595004	40.0	37.0	41.0	34.0	41.0
26	38.29037959776507	40.0	37.0	41.0	34.0	41.0
27	38.25732829122123	40.0	37.0	41.0	34.0	41.0
28	38.223088860683305	40.0	37.0	41.0	34.0	41.0
29	38.22465959200764	40.0	37.0	41.0	34.0	41.0
30	38.11673449092551	40.0	37.0	41.0	34.0	41.0
31	33.468953587270846	39.0	34.0	41.0	9.0	41.0
32	34.38563730814022	39.0	34.0	41.0	16.0	41.0
33	36.38002119780694	39.0	34.0	41.0	30.0	41.0
34	37.22923831830732	39.0	35.0	41.0	32.0	41.0
35	37.37201680122476	39.0	36.0	41.0	33.0	41.0
36	37.56559569763029	40.0	36.0	41.0	33.0	41.0
37	37.666832759771275	40.0	36.0	41.0	33.0	41.0
38	37.6317412297345	40.0	36.0	41.0	33.0	41.0
39	37.65311856378315	40.0	36.0	41.0	33.0	41.0
40	37.608009630608585	40.0	36.0	41.0	33.0	41.0
41	37.565662693168285	40.0	36.0	41.0	33.0	41.0
42	37.481560786674166	40.0	36.0	41.0	33.0	41.0
43	37.47888672258352	40.0	36.0	41.0	33.0	41.0
44	37.422168718841185	39.0	35.0	41.0	33.0	41.0
45	37.40543344281172	39.0	35.0	41.0	33.0	41.0
46	37.36404223859309	39.0	35.0	41.0	33.0	41.0
47	37.26584562239117	39.0	35.0	41.0	33.0	41.0
48	37.260643000143936	39.0	35.0	41.0	33.0	41.0
49	37.23898093505882	39.0	35.0	41.0	33.0	41.0
50	37.05119558248171	39.0	35.0	41.0	33.0	41.0
51	36.458137471703544	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	12.0
11	14.0
12	25.0
13	27.0
14	26.0
15	48.0
16	84.0
17	136.0
18	269.0
19	505.0
20	872.0
21	1463.0
22	2355.0
23	3684.0
24	5380.0
25	6869.0
26	8673.0
27	10669.0
28	13067.0
29	17233.0
30	23077.0
31	33534.0
32	52866.0
33	63992.0
34	118716.0
35	173219.0
36	226210.0
37	225119.0
38	343721.0
39	578189.0
40	515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.1763343496068	21.550789683733953	26.68317129659919	11.58970467006006
2	30.34070894887665	24.89098831503605	26.322337516192768	18.445965219894532
3	31.00930348193607	24.692461693469244	27.101474686939795	17.196760137654895
4	28.353401462910384	25.834212213600615	26.61889745233765	19.193488871151352
5	22.093767583057456	39.37003258181437	21.164832576580352	17.37136725854782
6	26.729597110817426	32.87580963845962	24.0907580178742	16.303835232848748
7	80.12294728026903	5.626473705559844	8.619761066694581	5.630817947476545
8	80.75485128822476	4.6624183819007365	8.451905839864962	6.1308244900095525
9	74.73797155306649	6.898551483192232	11.082056448974784	7.281420514766497
10	39.91463302932363	26.489774020909934	17.86148149117412	15.73411145859231
11	26.8256938356254	26.074611046412727	26.173324784423535	20.926370333538333
12	23.794669144105832	23.381809141227116	31.25017337712469	21.573348337542363
13	22.122188346440208	24.717323318896145	33.02367088441961	20.13681745024404
14	19.70501027177682	27.15439069390105	31.36888109600513	21.771717938316996
15	18.535310050639207	26.152545699593055	34.07487274773302	21.237271502034726
16	20.80891878099525	27.66366146317208	29.679913115161664	21.847506640671003
17	20.814728550305535	26.387134763094878	28.71167057037803	24.086466116221555
18	21.345929890216297	25.567119846119624	31.226567918035148	21.860382345628935
19	21.922039176687647	26.598327728563394	28.475458958690446	23.004174136058516
20	22.791672663988592	27.677112911034634	28.90108998599898	20.630124438977795
21	22.175680096306085	27.22243303717467	30.04106093715243	20.560825929366814
22	21.01932664250291	24.675974510291404	29.175614670976014	25.12908417622967
23	20.04710623765097	26.630831032542556	29.728746581526504	23.593316148279968
24	20.909883150360496	25.27390968687437	30.18536304515656	23.630844117608575
25	19.626761577012157	27.567983460476558	27.87694803920286	24.928306923308426
26	19.144079661881893	27.277233293641967	29.66986378446279	23.908823260013346
27	20.666605603025268	26.620834042107745	29.65013150491344	23.062428849953548
28	18.1584078091674	25.616057993012575	31.26435758868403	24.961176609135993
29	19.40839799536789	23.745835677740995	31.39976185179854	25.446004475092575
30	20.79311202125015	24.729256899101056	31.377307878518245	23.10032320113055
31	23.16642895463408	26.808264527694543	26.941365819190562	23.083940698480824
32	21.117307616816927	26.396608350889128	28.114991560132417	24.37109247216152
33	22.96266830666161	24.718527144969446	28.175601585909998	24.143202962458947
34	20.40019365897701	23.900030095651832	29.741151224107927	25.95862502126323
35	19.530298470355785	25.16567002080525	29.9828062232574	25.321225285581566
36	22.859715007262214	25.15567303037044	30.717611190348453	21.267000772018896
37	20.513143948811223	25.32305719482355	31.162189393245487	23.001609463119742
38	20.900618923622467	27.204846708451647	27.568820904701465	24.32571346322442
39	23.96875286235819	23.554898394462402	27.365478978841452	25.110869764337963
40	22.138675529618045	23.831621370529817	29.690538188765164	24.33916491108698
41	19.93912827290214	25.60370569069521	28.04197689177342	26.415189144629238
42	21.990029179697213	24.158852701411877	29.948889732148697	23.902228386742213
43	22.547767033484682	22.672807924315975	30.83940698480824	23.9400180573911
44	20.9868756787878	22.946233463747827	29.376601284953484	26.690289572510896
45	21.398322494536984	22.010912945055807	29.24119702183898	27.34956753856823
46	23.333394396974732	23.02259790900645	29.31724742551326	24.326760268505552
47	19.212750088324196	23.78409641076639	32.113892414587234	24.889261086322183
48	20.306504586315636	22.962144904021038	31.53919631524541	25.19215419441791
49	22.402522800727528	20.80551666383157	32.409562566243146	24.382397969197754
50	20.598667940279757	21.09506300459286	31.679468222917183	26.6268008322102
51	20.23369927901286	20.816141737435064	29.0997212880939	29.850437695458176
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1405.0
1	1744.0
2	2083.0
3	2914.5
4	3746.0
5	3050.0
6	2354.0
7	2397.0
8	2440.0
9	2768.0
10	3096.0
11	3375.0
12	3654.0
13	3789.5
14	3925.0
15	3900.5
16	3876.0
17	4246.5
18	4617.0
19	4799.5
20	4982.0
21	5409.0
22	5836.0
23	7347.5
24	8859.0
25	9866.0
26	13692.5
27	16512.0
28	19677.0
29	22842.0
30	26755.0
31	30668.0
32	33717.0
33	36766.0
34	42953.0
35	49140.0
36	54874.5
37	60609.0
38	66347.5
39	72086.0
40	78579.5
41	85073.0
42	96257.5
43	107442.0
44	123404.5
45	139367.0
46	162202.0
47	185037.0
48	207659.5
49	230282.0
50	221000.5
51	211719.0
52	179763.5
53	147808.0
54	128062.5
55	108317.0
56	94917.5
57	81518.0
58	75327.0
59	69136.0
60	63400.5
61	57665.0
62	50840.0
63	44015.0
64	38432.5
65	32850.0
66	27821.0
67	22792.0
68	18184.5
69	13577.0
70	11652.0
71	9727.0
72	8250.0
73	6773.0
74	5196.0
75	2743.0
76	1867.0
77	1432.5
78	998.0
79	676.5
80	355.0
81	266.0
82	177.0
83	110.5
84	44.0
85	31.5
86	19.0
87	23.0
88	27.0
89	14.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1910575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.038527009676052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.59279914355204	16.17308545986408
2	14.257607004001965	7.139789561260994
3	6.384494498353285	4.795750138204407
4	3.4385139440601424	3.4438129704599043
5	2.1462402282277337	2.6869346961866705
6	1.3246153314662037	1.989985005260852
7	0.9413422696945827	1.6498876691568298
8	0.6507778610935835	1.3035615241832719
9	0.5113713601018389	1.1523587050696207
>10	4.075681320112764	24.37426329242875
>50	1.5049818712939513	25.710515877811424
>100	0.16096956315566707	6.098298247428645
>500	0.007070403257558995	1.262831711593673
>1k	0.002828161303023598	1.098309524120628
>5k	7.070403257558995E-4	1.1206156169702732
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT	6860	0.35905421142849664	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	6146	0.3216832628920613	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	5984	0.3132041401148869	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC	2692	0.1408999908404538	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	2431	0.1272391819216728	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3747039503814297	0.0
2	0.0	0.0	0.0	1.273805006346257	0.0
3	0.0	0.0	0.0	1.8367245462753359	0.0
4	0.0	0.0	0.0	2.5564031770540283	0.0
5	0.0	0.0	0.0	4.344451277756696	0.0
6	0.0	0.0	0.0	5.558431362286223	0.0
7	0.0	0.0	0.0	6.684636823992777	0.0
8	0.0	0.0	0.0	8.569043350823705	0.0
9	0.0	0.0	0.0	9.22261622809887	0.0
10	0.0	0.0	0.0	10.593198382685841	0.0
11	0.0	0.0	0.0	12.780288656556273	0.0
12	5.234026405663217E-5	0.0	0.0	14.605707705795377	0.0
13	5.234026405663217E-5	0.0	0.0	15.297436635567827	0.0
14	5.234026405663217E-5	0.0	0.0	15.578032791175431	0.0
15	5.234026405663217E-5	0.0	0.0	16.08840270599165	0.0
16	5.234026405663217E-5	0.0	0.0	17.12955523860618	0.0
17	5.234026405663217E-5	0.0	0.0	18.35421273700326	0.0
18	5.234026405663217E-5	0.0	0.0	19.761537756957985	0.0
19	5.234026405663217E-5	0.0	0.0	20.649595017206863	0.0
20	5.234026405663217E-5	0.0	0.0	21.472802690289573	0.0
21	5.234026405663217E-5	0.0	0.0	22.34955445350222	0.0
22	5.234026405663217E-5	0.0	0.0	23.260013346767334	0.0
23	5.234026405663217E-5	0.0	0.0	24.139329782918754	0.0
24	5.234026405663217E-5	0.0	0.0	24.852518220954426	0.0
25	5.234026405663217E-5	0.0	0.0	25.4919592269343	0.0
26	5.234026405663217E-5	0.0	0.0	26.057495780066212	0.0
27	5.234026405663217E-5	0.0	0.0	26.624079138479253	0.0
28	1.0468052811326434E-4	0.0	0.0	27.22044410714052	0.0
29	1.0468052811326434E-4	0.0	0.0	27.870039124347382	0.0
30	1.0468052811326434E-4	0.0	0.0	28.573858655116915	0.0
31	1.0468052811326434E-4	0.0	0.0	29.183727411904794	0.0
32	1.0468052811326434E-4	0.0	0.0	29.83405519280845	0.0
33	1.0468052811326434E-4	0.0	0.0	30.46622090208445	0.0
34	1.0468052811326434E-4	0.0	0.0	31.084202399801107	0.0
35	1.0468052811326434E-4	0.0	0.0	31.771901129241197	0.0
36	2.0936105622652867E-4	0.0	0.0	32.4304986718658	0.0
37	2.0936105622652867E-4	0.0	0.0	33.043560184761134	0.0
38	2.617013202831608E-4	0.0	0.0	33.69305052143988	0.0
39	2.617013202831608E-4	0.0	0.0	34.37917904295827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	25	3.89178E-5	45.0	36
CGCTATA	20	7.034461E-4	45.0	31
TATTACG	35	1.212502E-7	45.0	1
ATCGAGT	25	3.89178E-5	45.0	28
AACGTAC	30	2.16626E-6	44.999996	33
TATAGCG	85	0.0	42.35294	1
GTTTCGC	265	0.0	41.60377	31
ACGTAAG	65	0.0	41.53846	1
CGTTAGG	325	0.0	39.461536	2
ACGGTTT	280	0.0	39.375	28
GCTACGA	180	0.0	38.750004	10
CGATTAC	35	6.250464E-6	38.571426	9
CTATTCG	35	6.250464E-6	38.571426	16
TAGTACG	70	0.0	38.571426	1
AACGCTT	215	0.0	37.67442	31
CACCGTA	30	1.1402051E-4	37.499996	14
TACGTCA	30	1.1402051E-4	37.499996	19
ACGCATC	235	0.0	37.340427	29
TTACGCA	310	0.0	37.01613	29
CGTAAGG	225	0.0	37.000004	2
>>END_MODULE