FastQCFastQC Report
Sat 14 Jan 2017
SRR2935078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4372745
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC69340.15857316170963548No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT63060.14421147357094913No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52940.12106811625191957No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG49130.11235505386204775No Hit
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC48600.11114300056371913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG5200.039.3750041
CGTAAGG9900.039.318182
TACGGGA25700.037.821014
TAAGGGA50600.036.9960484
CGTATGG5850.036.9230772
CGTTTTT42050.036.3852581
CACAACG18000.036.2512
ACGGGAG30300.036.0891085
TAGTACG1950.035.769231
TAGACGG7500.035.72
ACAACGA18450.035.36585613
CTACGAA3500.035.35714311
TATCGCG2300.035.217391
ACGGGAT33350.035.082465
ACGTACG1100.034.7727281
TCGTAAG1950.034.6153831
TTTACGG5900.034.3220332
CGTTAGG5450.034.2660562
TTACGGG16850.034.1839753
ATGGGCG12100.034.0289235
Produced by FastQC (version 0.11.5)