##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935078.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4372745
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13818413833873	33.0	31.0	34.0	30.0	34.0
2	32.45737791707497	34.0	31.0	34.0	30.0	34.0
3	32.50930090823956	34.0	31.0	34.0	31.0	34.0
4	35.99417642693548	37.0	35.0	37.0	35.0	37.0
5	30.486159609124247	37.0	35.0	37.0	0.0	37.0
6	32.956388264122424	37.0	35.0	37.0	17.0	37.0
7	35.17663046896172	37.0	35.0	37.0	32.0	37.0
8	35.75495438220157	37.0	35.0	37.0	35.0	37.0
9	37.65663536291277	39.0	38.0	39.0	35.0	39.0
10	37.48979668377644	39.0	37.0	39.0	35.0	39.0
11	37.36245790687543	39.0	37.0	39.0	35.0	39.0
12	37.21871867671223	39.0	37.0	39.0	34.0	39.0
13	37.18724279600114	39.0	37.0	39.0	34.0	39.0
14	38.28573676260564	40.0	38.0	41.0	33.0	41.0
15	38.4267920951256	40.0	38.0	41.0	34.0	41.0
16	38.53944673197271	40.0	38.0	41.0	34.0	41.0
17	38.55506552520213	40.0	38.0	41.0	34.0	41.0
18	38.51058316915347	40.0	38.0	41.0	34.0	41.0
19	38.496346116684144	40.0	38.0	41.0	34.0	41.0
20	38.43214982808282	40.0	38.0	41.0	34.0	41.0
21	38.38494195293803	40.0	37.0	41.0	34.0	41.0
22	38.4129090536951	40.0	37.0	41.0	34.0	41.0
23	38.33569531266973	40.0	37.0	41.0	34.0	41.0
24	38.326378967902315	40.0	37.0	41.0	34.0	41.0
25	38.2564942616137	40.0	37.0	41.0	34.0	41.0
26	38.216026317564825	40.0	37.0	41.0	34.0	41.0
27	38.1678675523041	40.0	37.0	41.0	34.0	41.0
28	38.11832201511865	40.0	37.0	41.0	34.0	41.0
29	38.09959053180554	40.0	37.0	41.0	34.0	41.0
30	37.97015764697004	40.0	36.0	41.0	34.0	41.0
31	33.388915886931436	39.0	34.0	41.0	9.0	41.0
32	34.300965183197285	39.0	34.0	41.0	16.0	41.0
33	36.25570459745538	39.0	34.0	41.0	30.0	41.0
34	37.07820259356537	39.0	35.0	41.0	31.0	41.0
35	37.22167357117783	39.0	35.0	41.0	32.0	41.0
36	37.41294061281872	40.0	36.0	41.0	33.0	41.0
37	37.530222320304524	40.0	36.0	41.0	33.0	41.0
38	37.503195132576906	40.0	36.0	41.0	33.0	41.0
39	37.51529028104772	40.0	36.0	41.0	33.0	41.0
40	37.49154432741905	40.0	35.0	41.0	33.0	41.0
41	37.42436958935406	40.0	35.0	41.0	33.0	41.0
42	37.35227117062623	40.0	35.0	41.0	33.0	41.0
43	37.351119719992816	40.0	35.0	41.0	33.0	41.0
44	37.29598433020906	39.0	35.0	41.0	33.0	41.0
45	37.240794741060824	39.0	35.0	41.0	33.0	41.0
46	37.211492323471866	39.0	35.0	41.0	33.0	41.0
47	37.12399465324413	39.0	35.0	41.0	33.0	41.0
48	37.10953051229834	39.0	35.0	41.0	33.0	41.0
49	37.08988495784684	39.0	35.0	41.0	33.0	41.0
50	36.90554034136452	39.0	35.0	41.0	32.0	41.0
51	36.3002841464572	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	17.0
11	20.0
12	28.0
13	44.0
14	63.0
15	122.0
16	206.0
17	368.0
18	720.0
19	1273.0
20	2079.0
21	3450.0
22	5540.0
23	9024.0
24	13187.0
25	17916.0
26	23152.0
27	28547.0
28	35080.0
29	44881.0
30	59491.0
31	85912.0
32	124675.0
33	155278.0
34	287089.0
35	369641.0
36	496196.0
37	513057.0
38	761761.0
39	1332414.0
40	1501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.91648495395913	21.32793016743487	25.919233799364015	10.836351079241986
2	31.659632564899166	23.411038146518948	26.44023376620407	18.48909552237782
3	32.23446599332913	24.115446933219292	26.31845671311728	17.331630360334298
4	28.039663872464548	26.13747199985364	26.28344438104669	19.539419746635122
5	22.771074005001434	39.47893142636948	21.001842092324157	16.74815247630493
6	27.941944933903073	33.10483460617987	23.54216859204001	15.411051867877044
7	82.87295508885151	5.411680763456364	7.086807028536994	4.62855711915513
8	84.9751586246168	4.123130893752093	6.642738142745575	4.2589723388855285
9	78.85435807484772	6.590070996593673	8.864180280350215	5.691390648208391
10	40.34632707829979	31.04555605231954	15.100857699225545	13.50725917015513
11	29.526052856958273	24.33935662838789	27.044110735933607	19.090479778720233
12	25.90276816965087	23.37684909593402	30.11561387640944	20.60476885800567
13	25.25672089271156	23.248051281288983	31.925415271185493	19.569812554813968
14	20.912104410387524	26.405518730225523	31.531200653136644	21.15117620625031
15	20.090080715888988	26.389899250928195	32.770673798723685	20.749346234459132
16	25.140249431421225	26.59233502067923	28.489930238328554	19.77748530957099
17	24.925555915105956	25.765691802288952	27.68915177994601	21.619600502659083
18	25.35446270020319	24.141860547550795	29.42389734594631	21.079779406299703
19	23.875917758753367	27.025564033576167	27.5415557047118	21.55696250295867
20	24.43574916900025	28.41720704042884	27.43532952413187	19.711714266439046
21	23.08147399402435	27.337496240919606	30.380573301210113	19.200456463845935
22	23.5914282675985	23.55243674168057	30.225133182932	22.631001807788927
23	20.945195752324913	27.26436140227706	30.09231043657931	21.698132408818715
24	21.766693461429835	26.03627698390828	29.633834124788894	22.563195429872998
25	21.882433116955138	27.81360449786118	27.411934608581106	22.892027776602568
26	21.118336422544647	27.04280720691465	27.520104648224397	24.318751722316303
27	21.889065106700713	26.476709709804712	28.776249243895997	22.85797593959858
28	20.177737325181322	27.196257728269085	28.203588363830956	24.422416582718636
29	21.80028791983068	27.032699139785194	26.901614432124443	24.265398508259686
30	23.498031556836725	26.545293631345984	27.49799039276244	22.45868441905485
31	25.850535533171954	27.26143417921694	24.892670393539984	21.995359894071115
32	23.2685875805701	27.82140280304477	25.904368994761874	23.00564062162326
33	23.85860597862441	26.852194674054857	26.34219466262039	22.947004684700342
34	22.178379942118738	25.947339714527146	28.136262233448328	23.73801810990579
35	20.79933771578265	28.88053156541257	27.02933740705209	23.290793311752687
36	22.856672410579623	28.588563934096317	26.50289463483464	22.051869020489416
37	21.6180454154084	28.83440493328561	27.009555782466162	22.537993868839827
38	22.47999826196131	28.835960020536298	26.62908539144176	22.054956326060633
39	22.723323678833317	25.828101112687797	27.188253602714084	24.2603216057648
40	23.43866381414878	25.244371670426702	28.096881935717725	23.220082579706798
41	20.377954808707116	26.68241573656822	27.33209917340252	25.607530281322145
42	21.770215276674033	26.172004084390927	28.91641291682913	23.141367722105908
43	22.024037532488176	25.60092116050673	29.136800796753526	23.238240510251572
44	22.598162024083273	25.25676663057187	27.41625683638081	24.72881450896405
45	21.948181291156928	24.347886739336506	27.183954243844543	26.519977725662024
46	22.367368780937376	25.584455530793587	27.06064497243722	24.987530715831817
47	19.713703863362717	27.07978626697875	29.279114149121433	23.9273957205371
48	20.366017227165088	27.418589467256837	28.4977514124423	23.717641893135777
49	23.297379563637943	24.833028223690153	28.871795634092546	22.99779657857936
50	20.824905179698337	25.529318540184715	28.471177715599698	25.174598564517254
51	20.36496525637786	24.715321840171335	27.191935500469384	27.727777402981424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1320.0
1	2536.5
2	3753.0
3	7804.0
4	11855.0
5	9002.0
6	6149.0
7	5787.0
8	5425.0
9	5613.5
10	5802.0
11	6231.0
12	6660.0
13	6913.5
14	7167.0
15	7158.0
16	7149.0
17	7636.0
18	8123.0
19	8757.0
20	9391.0
21	10531.0
22	11671.0
23	14814.0
24	17957.0
25	22251.5
26	32374.5
27	38203.0
28	47273.0
29	56343.0
30	66203.0
31	76063.0
32	86194.5
33	96326.0
34	110882.0
35	125438.0
36	136084.5
37	146731.0
38	159053.5
39	171376.0
40	186756.0
41	202136.0
42	220395.0
43	238654.0
44	262942.0
45	287230.0
46	329248.5
47	371267.0
48	406569.5
49	441872.0
50	436732.5
51	431593.0
52	390607.5
53	349622.0
54	313189.0
55	276756.0
56	255150.0
57	233544.0
58	210965.5
59	188387.0
60	167655.0
61	146923.0
62	130317.5
63	113712.0
64	100570.0
65	87428.0
66	72734.5
67	58041.0
68	49818.0
69	41595.0
70	33784.5
71	25974.0
72	21749.0
73	17524.0
74	13237.0
75	7484.0
76	6018.0
77	4802.5
78	3587.0
79	2339.0
80	1091.0
81	922.0
82	753.0
83	572.5
84	392.0
85	275.5
86	159.0
87	116.0
88	73.0
89	42.5
90	12.0
91	7.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4372745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.219484973930985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.34031987528886	14.962672256086867
2	13.204407205408902	5.867902549804983
3	5.667227648755722	3.7776863875612197
4	2.97005874004705	2.639727021846713
5	1.7488182571645634	1.9428920493602095
6	1.0990477127364295	1.4652164483268193
7	0.7763848118836106	1.2075609463145285
8	0.5370149422225736	0.9545756351592714
9	0.41788619740077226	0.8356694475563654
>10	3.8230362091839805	19.82125900480978
>50	2.0065753804873037	32.218749936327434
>100	0.3992153663063514	11.511830561357403
>500	0.006096616264854701	0.9288462940089017
>1k	0.0035659453624395606	1.3916975512106249
>5k	3.450914866876994E-4	0.4737139102688287
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	6934	0.15857316170963548	No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT	6306	0.14421147357094913	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5294	0.12106811625191957	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	4913	0.11235505386204775	No Hit
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	4860	0.11114300056371913	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.860679047143156E-5	0.0	0.0	0.15381642423694955	0.0
2	6.860679047143156E-5	0.0	0.0	0.5521474497140813	0.0
3	6.860679047143156E-5	0.0	0.0	0.8248137039777074	0.0
4	6.860679047143156E-5	0.0	0.0	1.2132424826967958	0.0
5	6.860679047143156E-5	0.0	0.0	2.263109328350956	0.0
6	6.860679047143156E-5	0.0	0.0	3.0825717026718915	0.0
7	6.860679047143156E-5	0.0	0.0	3.77085789361145	0.0
8	6.860679047143156E-5	0.0	0.0	5.021674028556433	0.0
9	6.860679047143156E-5	0.0	0.0	5.482871743035553	0.0
10	6.860679047143156E-5	0.0	0.0	6.413362773269422	0.0
11	6.860679047143156E-5	0.0	0.0	7.782571359637939	0.0
12	9.147572062857541E-5	0.0	0.0	8.935211177418303	0.0
13	9.147572062857541E-5	0.0	0.0	9.369103389289794	0.0
14	9.147572062857541E-5	0.0	0.0	9.561362485120902	0.0
15	9.147572062857541E-5	0.0	0.0	9.90215528232266	0.0
16	1.372135809428631E-4	0.0	0.0	10.547653704938202	0.0
17	1.372135809428631E-4	0.0	0.0	11.36645288028458	0.0
18	1.6008251110000697E-4	0.0	0.0	12.222253984625219	0.0
19	1.6008251110000697E-4	0.0	0.0	12.830133017132258	0.0
20	1.6008251110000697E-4	0.0	0.0	13.362361628679468	0.0
21	1.6008251110000697E-4	0.0	0.0	13.985562844391795	0.0
22	1.6008251110000697E-4	0.0	0.0	14.665753434055725	0.0
23	1.6008251110000697E-4	0.0	0.0	15.317586550324796	0.0
24	1.6008251110000697E-4	0.0	0.0	15.83135993523519	0.0
25	1.8295144125715082E-4	0.0	0.0	16.31947438050927	0.0
26	1.8295144125715082E-4	0.0	0.0	16.7653499117831	0.0
27	1.8295144125715082E-4	0.0	0.0	17.235100606140993	0.0
28	1.8295144125715082E-4	0.0	0.0	17.700849237721386	0.0
29	1.8295144125715082E-4	0.0	0.0	18.222375189955052	0.0
30	1.8295144125715082E-4	0.0	0.0	18.84322090586119	0.0
31	2.0582037141429467E-4	0.0	0.0	19.341374811474257	0.0
32	2.0582037141429467E-4	0.0	0.0	19.870013000986795	0.0
33	2.0582037141429467E-4	0.0	0.0	20.447613569965778	0.0
34	2.0582037141429467E-4	0.0	0.0	21.0109439265267	0.0
35	2.2868930157143855E-4	0.0	0.0	21.616421721367242	0.0
36	2.515582317285824E-4	0.0	0.0	22.16321784142455	0.0
37	2.515582317285824E-4	0.0	0.0	22.73141928010895	0.0
38	2.744271618857262E-4	0.0	0.0	23.400175404694306	0.0
39	2.744271618857262E-4	0.0	0.0	24.337893016857834	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	520	0.0	39.375004	1
CGTAAGG	990	0.0	39.31818	2
TACGGGA	2570	0.0	37.82101	4
TAAGGGA	5060	0.0	36.996048	4
CGTATGG	585	0.0	36.923077	2
CGTTTTT	4205	0.0	36.385258	1
CACAACG	1800	0.0	36.25	12
ACGGGAG	3030	0.0	36.089108	5
TAGTACG	195	0.0	35.76923	1
TAGACGG	750	0.0	35.7	2
ACAACGA	1845	0.0	35.365856	13
CTACGAA	350	0.0	35.357143	11
TATCGCG	230	0.0	35.21739	1
ACGGGAT	3335	0.0	35.08246	5
ACGTACG	110	0.0	34.772728	1
TCGTAAG	195	0.0	34.615383	1
TTTACGG	590	0.0	34.322033	2
CGTTAGG	545	0.0	34.266056	2
TTACGGG	1685	0.0	34.183975	3
ATGGGCG	1210	0.0	34.028923	5
>>END_MODULE