FastQCFastQC Report
Sat 14 Jan 2017
SRR2935077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3142904
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244420.7776884053728654No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT109370.3479902663269384No Hit
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT105640.3361222614499202TruSeq Adapter, Index 21 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC100900.321040668120948No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC92480.2942501584521831TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG76510.24343727966237594TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCC73180.2328419830831613No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT68540.21807856682864No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC65700.20904233791423474TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC46780.14884323542812633No Hit
CGTCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT42750.13602069932775546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT157150.043.911871
CGTTATT27800.043.2194251
CGCGAAA2750.042.54545626
CGTAAGG5150.040.194182
ACGCGAA3000.039.7525
CGTTTCT12700.039.5078741
CGCGAGA5000.039.1526
CGTTACT2650.039.0566021
CGCGTAA356.2517483E-638.57142631
ACGCGAT2400.038.43750425
TCGCGAC3000.038.2531
CGTTCTG11450.038.1222721
TATACGC2250.038.026
CACGCGA1550.037.74193631
TACGGGA18500.037.70274
CGATCGT301.1403723E-437.50000410
CGTTATC2250.037.01
CGCGATA2500.036.926
GCGTAAG2750.036.8181841
AAGGGAT52200.036.594835
Produced by FastQC (version 0.11.5)