SRR2935075.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935075.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109321
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	4023	0.36265427229809943	RNA PCR Primer, Index 41 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	3769	0.3397573831199445	Illumina PCR Primer Index 3 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	3627	0.32695676003609414	RNA PCR Primer, Index 41 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1199	0.10808413434884943	Illumina PCR Primer Index 3 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	30	2.1654323E-6	45.000004	1
CACGGAC	30	2.1654323E-6	45.000004	18
GTAATCG	30	2.1654323E-6	45.000004	1
AACGCGT	30	2.1654323E-6	45.000004	31
TCACGAG	25	3.8907187E-5	45.0	25
ACGTTAC	20	7.03318E-4	45.0	36
CGCATAC	25	3.8907187E-5	45.0	44
GTTAGCA	25	3.8907187E-5	45.0	11
GAATAGT	35	1.2118835E-7	45.0	17
CGTTACA	55	1.8189894E-12	45.0	28
TCGGACA	25	3.8907187E-5	45.0	21
TGCGCGA	25	3.8907187E-5	45.0	20
ATTCACG	35	1.2118835E-7	45.0	1
TACGATG	35	1.2118835E-7	45.0	1
TTAAACG	50	2.1827873E-11	45.0	1
CGGTCTA	25	3.8907187E-5	45.0	31
AACTCGT	20	7.03318E-4	45.0	22
CACGTTA	20	7.03318E-4	45.0	35
CGCGTTA	55	1.8189894E-12	45.0	26
CGACTTA	25	3.8907187E-5	45.0	34