FastQCFastQC Report
Sat 14 Jan 2017
SRR2935073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4151932
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73360.17668882823707133No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC59220.14263239378679612TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT58930.14193392377331804TruSeq Adapter, Index 20 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC48240.11618687396614394TruSeq Adapter, Index 23 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG8950.038.966482
CGTTTTT50450.038.0872151
CGACGTA250.00210774136.00000419
TACGGGT6700.035.9328354
TACGGGA16550.035.8912434
TTACGGG14400.035.156253
ACGGGCT13650.035.1098865
TACGGGC12050.034.917014
TAAGGGA36250.034.820694
CGTTAGG5650.034.646022
AACGGGA16700.034.6257484
ACGGGAG25500.033.970595
TACGAAT8150.033.6809812
AGTACGG8450.033.5502972
ACGGGTA7850.033.535035
TAAGGGC28050.033.2085574
ACGGGAC13350.033.2022485
TATTACG754.7293724E-1133.0000041
GCGTAAG5250.033.01
TTTACGG5400.032.9166642
Produced by FastQC (version 0.11.5)