##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935073.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4151932
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18865554638178	33.0	31.0	34.0	30.0	34.0
2	32.51505226964218	34.0	31.0	34.0	31.0	34.0
3	32.560609614993695	34.0	31.0	34.0	31.0	34.0
4	36.03985831174499	37.0	35.0	37.0	35.0	37.0
5	30.529790468630026	37.0	35.0	37.0	0.0	37.0
6	32.995409366049344	37.0	35.0	37.0	17.0	37.0
7	35.168600786332725	37.0	35.0	37.0	32.0	37.0
8	35.716122759235944	37.0	35.0	37.0	35.0	37.0
9	37.62487800859937	39.0	37.0	39.0	35.0	39.0
10	37.50535365222745	39.0	37.0	39.0	35.0	39.0
11	37.40417256351983	39.0	37.0	39.0	35.0	39.0
12	37.282333862886	39.0	37.0	39.0	34.0	39.0
13	37.263089568904306	39.0	37.0	39.0	34.0	39.0
14	38.48964048544148	40.0	38.0	41.0	34.0	41.0
15	38.603716534856545	40.0	38.0	41.0	35.0	41.0
16	38.68566489046545	40.0	38.0	41.0	35.0	41.0
17	38.67814887141697	40.0	38.0	41.0	35.0	41.0
18	38.63389742413893	40.0	38.0	41.0	35.0	41.0
19	38.60663638036461	40.0	38.0	41.0	35.0	41.0
20	38.521367883674394	40.0	38.0	41.0	34.0	41.0
21	38.47544010836401	40.0	38.0	41.0	34.0	41.0
22	38.47587026955162	40.0	38.0	41.0	34.0	41.0
23	38.4077911198931	40.0	37.0	41.0	34.0	41.0
24	38.37707337210725	40.0	37.0	41.0	34.0	41.0
25	38.307090048680955	40.0	37.0	41.0	34.0	41.0
26	38.25366552246039	40.0	37.0	41.0	34.0	41.0
27	38.21120817971007	40.0	37.0	41.0	34.0	41.0
28	38.14192139948342	40.0	37.0	41.0	34.0	41.0
29	38.13098576758964	40.0	37.0	41.0	34.0	41.0
30	38.01560960054259	40.0	37.0	41.0	34.0	41.0
31	33.41819278350416	39.0	34.0	41.0	9.0	41.0
32	34.340772199544695	39.0	34.0	41.0	16.0	41.0
33	36.27733161333085	39.0	34.0	41.0	30.0	41.0
34	37.10128224643371	39.0	35.0	41.0	32.0	41.0
35	37.24235970145946	39.0	35.0	41.0	33.0	41.0
36	37.39857155656692	39.0	36.0	41.0	33.0	41.0
37	37.488908537037695	40.0	36.0	41.0	33.0	41.0
38	37.46113303397069	40.0	35.0	41.0	33.0	41.0
39	37.450165127945255	40.0	35.0	41.0	33.0	41.0
40	37.42757877537493	40.0	35.0	41.0	33.0	41.0
41	37.34915889759274	40.0	35.0	41.0	33.0	41.0
42	37.246673596773746	39.0	35.0	41.0	33.0	41.0
43	37.23153847413686	39.0	35.0	41.0	33.0	41.0
44	37.165607481047374	39.0	35.0	41.0	33.0	41.0
45	37.11886466348678	39.0	35.0	41.0	33.0	41.0
46	37.06298224537396	39.0	35.0	41.0	33.0	41.0
47	36.985007461586555	39.0	35.0	41.0	33.0	41.0
48	36.94735270230823	39.0	35.0	41.0	33.0	41.0
49	36.901507539140816	39.0	35.0	41.0	32.0	41.0
50	36.72669518672271	39.0	35.0	41.0	32.0	41.0
51	36.11360614769221	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	18.0
10	35.0
11	37.0
12	37.0
13	62.0
14	103.0
15	134.0
16	216.0
17	353.0
18	628.0
19	1245.0
20	1994.0
21	3224.0
22	5154.0
23	8351.0
24	12841.0
25	17737.0
26	23089.0
27	27519.0
28	33074.0
29	41372.0
30	54376.0
31	76347.0
32	114959.0
33	142299.0
34	263379.0
35	364272.0
36	485275.0
37	502368.0
38	716401.0
39	1253602.0
40	1423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.93578603888503	21.514490121707194	25.660319099638436	11.889404739769342
2	31.859168213737604	23.776569558460977	26.291302458710785	18.07295976909063
3	31.674314511894703	23.77153575732936	26.919636448766504	17.634513282009436
4	28.18909365567644	25.818269663375993	26.795694149133464	19.196942531814106
5	22.813644346776393	39.35683436048567	21.07418907631435	16.755332216423582
6	27.147771206272164	33.559003374814424	23.58181685056499	15.711408568348423
7	81.99886221643322	5.676875247475151	7.607807642321696	4.716454893769936
8	83.70674182525147	4.701714767968261	7.149081439676758	4.442461967103507
9	77.59503286662691	6.476647498080412	9.38083764377644	6.547481991516239
10	41.77457626955355	25.06430741158574	17.240263087160386	15.920853231700328
11	30.547658294981712	24.95585187811361	23.8211271282863	20.675362698618375
12	27.112968131462654	22.228591412383437	28.87720704481673	21.78123341133718
13	24.82343159762732	22.89994152120025	30.716615782724766	21.560011098447664
14	20.990420845042742	25.42317648747619	30.453051735914748	23.133350931566316
15	20.715079148695114	24.676969661352835	32.19279602845133	22.415155161500717
16	23.581600084009082	24.320364591712966	29.2804169239766	22.817618400301352
17	23.78673350141573	24.803031456199186	27.34170501829028	24.068530024094805
18	24.223469941222543	23.879967205628606	29.026077498379067	22.87048535476978
19	24.3317809636574	26.288580834175512	26.659877859271297	22.71976034289579
20	25.371489706478815	26.240217807035375	26.896731449358995	21.491561037126814
21	24.931742620062177	25.465277369667906	27.834993444015943	21.767986566253974
22	23.84362267975487	24.020913637313907	27.463720504093036	24.67174317883819
23	23.076148645979753	25.038777128334473	27.849059184977015	24.036015040708758
24	22.930144327989957	24.2241924964089	28.664221861051676	24.181441314549467
25	22.972967765368026	26.514042137491657	26.68678099737664	23.826209099763677
26	22.52406831325754	25.951460669394393	27.611434869357204	23.913036147990862
27	23.59296828560776	24.87914060249542	28.00014547444419	23.52774563745264
28	21.297169606824003	25.56065465426698	28.460894831610922	24.6812809072981
29	22.595890298781388	24.229756171343848	27.98759709937446	25.186756430500306
30	24.030258684390784	24.71569861934155	27.87446904236389	23.379573653903773
31	25.69555570755976	26.141420427887546	25.465301454840784	22.69772240971191
32	23.413076129377842	25.96244350822701	26.916866653885467	23.70761370850968
33	23.75867427501221	25.22998931581731	26.31073919322378	24.700597215946697
34	22.062981763670503	24.291558724950217	29.03007563707691	24.615383874302374
35	22.628357111821675	24.947807430372173	27.51747860995797	24.906356847848183
36	23.15476265025535	25.383845400165512	26.543618729786516	24.917773219792615
37	22.34550084153594	26.162735805885067	28.004673486945354	23.487089865633635
38	22.02719119677297	25.784429995481624	26.913831922102773	25.274546885642636
39	22.2576140457021	24.82465994144413	26.778641846735447	26.139084166118327
40	23.084771137870273	23.183544431845224	29.534635923709736	24.19704850657477
41	21.010242942321792	23.9680225976726	28.136226701208017	26.885507758797587
42	21.845251800848374	23.906677662350926	28.488954058014436	25.75911647878626
43	22.403883300593556	23.775557981200077	28.58584870850486	25.234710009701505
44	22.39111815896792	24.037291554871324	27.922735728812516	25.648854557348244
45	22.058670517725243	23.432705545273862	27.766904660288272	26.741719276712622
46	22.266670070704432	24.420558910887753	27.98114227304301	25.33162874536481
47	21.443414776542582	24.116170496048586	29.59954546461744	24.840869262791397
48	21.11833719820074	23.835963594779493	29.05770133036861	25.987997876651157
49	22.137862566149927	23.46705100179868	29.189712162915963	25.205374269135426
50	20.94646540453938	23.413076129377842	29.424325831925955	26.216132634156818
51	20.71165905414636	23.03204869443912	28.331942816019147	27.924349435395378
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1159.0
1	2183.5
2	3208.0
3	9011.0
4	14814.0
5	11001.5
6	7189.0
7	6659.5
8	6130.0
9	6224.0
10	6318.0
11	6600.5
12	6883.0
13	7003.0
14	7123.0
15	7100.5
16	7078.0
17	6818.0
18	6558.0
19	7408.0
20	8258.0
21	9167.5
22	10077.0
23	11227.5
24	12378.0
25	16058.0
26	25321.0
27	30904.0
28	35634.5
29	40365.0
30	50035.5
31	59706.0
32	68217.0
33	76728.0
34	89885.5
35	103043.0
36	114299.0
37	125555.0
38	135807.5
39	146060.0
40	160321.0
41	174582.0
42	192987.5
43	211393.0
44	234954.0
45	258515.0
46	293197.0
47	327879.0
48	355753.0
49	383627.0
50	376193.5
51	368760.0
52	333228.5
53	297697.0
54	272965.0
55	248233.0
56	238476.5
57	228720.0
58	214567.0
59	200414.0
60	193688.5
61	186963.0
62	173838.0
63	160713.0
64	143103.5
65	125494.0
66	109547.0
67	93600.0
68	81004.0
69	68408.0
70	57250.0
71	46092.0
72	38368.5
73	30645.0
74	24996.0
75	15129.0
76	10911.0
77	8258.0
78	5605.0
79	4016.5
80	2428.0
81	2024.5
82	1621.0
83	1165.5
84	710.0
85	436.0
86	162.0
87	109.5
88	57.0
89	41.5
90	26.0
91	40.5
92	55.0
93	29.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4151932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.48496747976754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.19692216697624	15.78375512104234
2	12.393931257987951	5.573542825646671
3	4.8401840192497385	3.264941408067627
4	2.4063095205957468	2.1642316526740153
5	1.4442770550211437	1.6237261306962423
6	0.8882024868887558	1.1982722419885397
7	0.6549952981234467	1.0309283584394442
8	0.4791459328929567	0.861886457532881
9	0.3667268423355176	0.7421257011484755
>10	3.862695587958112	21.562190090873383
>50	2.145398581630726	34.50631115661796
>100	0.312536949843897	9.026884881271128
>500	0.004939532226780444	0.7327053928562043
>1k	0.0033733390817025956	1.4097156404993656
>5k	3.614291873252781E-4	0.5187829406457216
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7336	0.17668882823707133	No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	5922	0.14263239378679612	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	5893	0.14193392377331804	TruSeq Adapter, Index 20 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	4824	0.11618687396614394	TruSeq Adapter, Index 23 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.225551863566166E-5	0.0	0.0	0.14940032736566977	0.0
2	7.225551863566166E-5	0.0	0.0	0.513110523004712	0.0
3	7.225551863566166E-5	0.0	0.0	0.7817083709463449	0.0
4	7.225551863566166E-5	0.0	0.0	1.1497298125306483	0.0
5	7.225551863566166E-5	0.0	0.0	2.1425688089303967	0.0
6	9.634069151421555E-5	0.0	0.0	3.0436432966628546	0.0
7	9.634069151421555E-5	0.0	0.0	3.747147111272535	0.0
8	9.634069151421555E-5	0.0	0.0	4.899140930053767	0.0
9	9.634069151421555E-5	0.0	0.0	5.3525443094925444	0.0
10	1.2042586439276944E-4	0.0	0.0	6.249114869896713	0.0
11	1.2042586439276944E-4	0.0	0.0	7.437742236626226	0.0
12	1.2042586439276944E-4	0.0	0.0	8.436963803838792	0.0
13	1.2042586439276944E-4	0.0	0.0	8.846387657601328	0.0
14	1.4451103727132332E-4	0.0	0.0	9.026424324868518	0.0
15	1.685962101498772E-4	0.0	0.0	9.327922518962257	0.0
16	1.685962101498772E-4	0.0	0.0	9.91996015348999	0.0
17	1.685962101498772E-4	0.0	0.0	10.605400088440755	0.0
18	1.926813830284311E-4	0.0	0.0	11.338071047406364	0.0
19	1.926813830284311E-4	0.0	0.0	11.835164930446838	0.0
20	1.926813830284311E-4	0.0	0.0	12.283558593926875	0.0
21	1.926813830284311E-4	0.0	0.0	12.81345648242794	0.0
22	2.16766555906985E-4	0.0	0.0	13.392175016353832	0.0
23	2.16766555906985E-4	0.0	0.0	13.949770853665234	0.0
24	2.16766555906985E-4	0.0	0.0	14.426199658375907	0.0
25	2.16766555906985E-4	0.0	0.0	14.847112139601515	0.0
26	2.16766555906985E-4	0.0	0.0	15.217325331917767	0.0
27	2.16766555906985E-4	0.0	0.0	15.606252703560656	0.0
28	2.4085172878553888E-4	0.0	0.0	15.984124980852288	0.0
29	2.4085172878553888E-4	0.0	0.0	16.412238928768584	0.0
30	2.4085172878553888E-4	0.0	0.0	16.88881224451653	0.0
31	2.4085172878553888E-4	0.0	0.0	17.282243543487706	0.0
32	2.4085172878553888E-4	0.0	0.0	17.7493273011215	0.0
33	2.4085172878553888E-4	0.0	0.0	18.199984970852125	0.0
34	2.4085172878553888E-4	0.0	0.0	18.655050227219522	0.0
35	2.4085172878553888E-4	0.0	0.0	19.163608652550185	0.0
36	2.6493690166409274E-4	0.0	0.0	19.629464066367177	0.0
37	2.6493690166409274E-4	0.0	0.0	20.099197193017613	0.0
38	2.6493690166409274E-4	0.0	0.0	20.588680161428464	0.0
39	2.6493690166409274E-4	0.0	0.0	21.185077212247215	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	895	0.0	38.96648	2
CGTTTTT	5045	0.0	38.087215	1
CGACGTA	25	0.002107741	36.000004	19
TACGGGT	670	0.0	35.932835	4
TACGGGA	1655	0.0	35.891243	4
TTACGGG	1440	0.0	35.15625	3
ACGGGCT	1365	0.0	35.109886	5
TACGGGC	1205	0.0	34.91701	4
TAAGGGA	3625	0.0	34.82069	4
CGTTAGG	565	0.0	34.64602	2
AACGGGA	1670	0.0	34.625748	4
ACGGGAG	2550	0.0	33.97059	5
TACGAAT	815	0.0	33.68098	12
AGTACGG	845	0.0	33.550297	2
ACGGGTA	785	0.0	33.53503	5
TAAGGGC	2805	0.0	33.208557	4
ACGGGAC	1335	0.0	33.202248	5
TATTACG	75	4.7293724E-11	33.000004	1
GCGTAAG	525	0.0	33.0	1
TTTACGG	540	0.0	32.916664	2
>>END_MODULE