##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935071.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1483655
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03744603698299	33.0	31.0	34.0	30.0	34.0
2	32.36571103120335	34.0	31.0	34.0	30.0	34.0
3	32.42405747967014	34.0	31.0	34.0	30.0	34.0
4	35.93189184817225	37.0	35.0	37.0	35.0	37.0
5	30.403510250024432	37.0	35.0	37.0	0.0	37.0
6	32.880649477135854	37.0	35.0	37.0	17.0	37.0
7	35.08041559526979	37.0	35.0	37.0	32.0	37.0
8	35.66115707492645	37.0	35.0	37.0	35.0	37.0
9	37.57463089464869	39.0	37.0	39.0	35.0	39.0
10	37.46185872052465	39.0	37.0	39.0	35.0	39.0
11	37.32298479093859	39.0	37.0	39.0	35.0	39.0
12	37.11247426119954	39.0	37.0	39.0	34.0	39.0
13	36.997766327077386	39.0	37.0	39.0	33.0	39.0
14	38.00299867556811	40.0	37.0	41.0	33.0	41.0
15	38.16842662209206	40.0	37.0	41.0	33.0	41.0
16	38.304806036443786	40.0	37.0	41.0	34.0	41.0
17	38.33187701992714	40.0	37.0	41.0	34.0	41.0
18	38.28652213621091	40.0	37.0	41.0	34.0	41.0
19	38.28281372691091	40.0	37.0	41.0	34.0	41.0
20	38.207151258210295	40.0	37.0	41.0	34.0	41.0
21	38.14592678216971	40.0	37.0	41.0	34.0	41.0
22	38.1663533638211	40.0	36.0	41.0	34.0	41.0
23	38.11314288025181	40.0	36.0	41.0	34.0	41.0
24	38.092572734227296	40.0	36.0	41.0	34.0	41.0
25	38.03566866960311	40.0	36.0	41.0	34.0	41.0
26	37.97047157189508	40.0	36.0	41.0	34.0	41.0
27	37.929935867839895	40.0	36.0	41.0	34.0	41.0
28	37.874735029370036	40.0	36.0	41.0	34.0	41.0
29	37.87213536839764	40.0	36.0	41.0	34.0	41.0
30	37.753791818178755	40.0	36.0	41.0	33.0	41.0
31	33.109510634210785	38.0	33.0	41.0	9.0	41.0
32	34.001090550026795	38.0	33.0	41.0	16.0	41.0
33	35.96467777212357	38.0	34.0	41.0	29.0	41.0
34	36.8061240652308	38.0	35.0	41.0	31.0	41.0
35	36.93621225958865	39.0	35.0	41.0	32.0	41.0
36	37.08330575504413	39.0	35.0	41.0	32.0	41.0
37	37.1787464066781	39.0	35.0	41.0	32.0	41.0
38	37.151153064560155	39.0	35.0	41.0	32.0	41.0
39	37.14368434710226	39.0	35.0	41.0	33.0	41.0
40	37.122102510354495	39.0	35.0	41.0	33.0	41.0
41	37.0284439441784	39.0	35.0	41.0	32.0	41.0
42	36.92149320428267	39.0	35.0	41.0	32.0	41.0
43	36.903714138394704	39.0	35.0	41.0	32.0	41.0
44	36.84182306533527	39.0	35.0	41.0	32.0	41.0
45	36.82180493443557	39.0	35.0	41.0	32.0	41.0
46	36.76075772332516	39.0	35.0	41.0	32.0	41.0
47	36.642461353886176	39.0	35.0	41.0	32.0	41.0
48	36.62070360023051	39.0	35.0	41.0	32.0	41.0
49	36.57993401430926	38.0	35.0	40.0	32.0	41.0
50	36.39104104390846	38.0	35.0	40.0	31.0	41.0
51	35.71671042122326	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	8.0
11	12.0
12	19.0
13	32.0
14	25.0
15	50.0
16	91.0
17	188.0
18	297.0
19	556.0
20	888.0
21	1493.0
22	2337.0
23	3741.0
24	5337.0
25	7025.0
26	8638.0
27	10595.0
28	12971.0
29	16775.0
30	22317.0
31	32764.0
32	49441.0
33	58474.0
34	112758.0
35	142066.0
36	170683.0
37	171787.0
38	254971.0
39	396986.0
40	324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.93626887652453	20.841637712271382	25.57973383300026	12.642359578203827
2	31.197818899946416	24.12070191520266	26.405599684562787	18.275879500288138
3	31.669424495586913	23.899828464164514	26.94480859768612	17.485938442562453
4	27.887413178939845	25.737991649001955	27.170602330056514	19.20399284200168
5	22.400827685681644	38.443303867812936	22.070966633078445	17.084901813426974
6	27.647263009257543	32.13031331407908	24.22941991231115	15.993003764352226
7	79.69561656854188	6.147925225203973	8.547202685260388	5.609255520993762
8	79.89013618395113	4.822819321203379	8.885691080473562	6.40135341437194
9	74.53161280755971	6.617508787420256	11.41350246519575	7.437375939824285
10	42.12111306199892	24.159053149148555	17.920069018740882	15.799764770111649
11	31.45738059050116	24.18237393464114	24.491205839632528	19.869039635225167
12	27.856139061978695	21.478982647583162	29.82802605727073	20.836852233167413
13	24.867236655421916	23.369583899221855	32.08724400214336	19.67593544321288
14	20.366257654239025	26.229817578884578	31.79883463473651	21.605090132139885
15	18.50551509616454	25.265172833306938	35.03968240595017	21.189629664578355
16	23.523595445032704	25.176675170440564	29.896640391465674	21.403088993061058
17	24.05471622445919	24.64973326009079	26.62364228880703	24.671908226642987
18	24.296618823109146	23.78430295452784	30.14764214052458	21.771436081838434
19	24.595677566550176	25.264970629964512	27.80700364977033	22.33234815371498
20	24.93430076399163	27.89246826250038	26.937933684043795	20.235297289464192
21	23.688391169106023	26.578618344561235	30.094664864810216	19.638325621522522
22	22.715253883146687	23.974171893061392	28.404042718826144	24.906531504965777
23	21.317152572532024	25.941408211477736	29.213462698538407	23.527976517451833
24	23.155180955141187	25.21259996427741	28.127563348622154	23.504655731959247
25	22.66301801968787	26.04338609717219	26.779204060243117	24.514391822896833
26	21.637510068041426	26.22840215548763	27.63722024325062	24.496867533220325
27	23.064930863307172	25.219542279033874	28.790857712877994	22.924669144780964
28	19.503792997698252	25.270430120209888	30.171973942729274	25.053802939362583
29	21.22265621050716	24.642184335307064	30.03703691222016	24.09812254196562
30	25.073753669148154	23.688458570220167	28.27894625098153	22.958841509650156
31	26.851727659058206	25.238077585422484	26.286097509191826	21.62409724632748
32	25.643427885862952	24.393811229699626	27.718168981333264	22.24459190310416
33	25.508153849783138	24.604035304703586	25.934802902291977	23.9530079432213
34	23.497983021659348	23.30272199399456	30.304147527558627	22.89514745678746
35	23.75262443088184	24.883143318359053	28.99568969875072	22.368542552008385
36	25.425183078276287	26.228806562172473	26.93402441942365	21.41198594012759
37	24.534005547111693	26.23547927247237	28.61905227293407	20.61146290748186
38	24.02209408521523	25.669849122606	27.623268212623554	22.68478857955522
39	23.758016520013076	23.73489793786291	28.41961237619258	24.087473165931435
40	26.77293575662806	22.26892370530885	28.145829050554205	22.81231148750889
41	23.838560851410872	23.775945216374428	25.999238367410214	26.386255564804483
42	23.92685631093482	23.607981639936508	29.212519082940442	23.25264296618823
43	23.49616319157756	23.032308724063206	29.968624781367637	23.502903302991598
44	22.57950803926789	22.204825245761313	29.339367979752705	25.876298735218096
45	22.822826061314792	20.96720598791498	28.38645102803549	27.823516922734733
46	25.024483454711504	21.607718775591362	28.78843127276894	24.579366496928195
47	21.71414513481908	23.46785472363858	31.21143392500278	23.60656621653956
48	21.620457586163898	23.335681138809225	30.3025973019334	24.741263973093474
49	24.141461458357906	21.200953051753945	31.598720726853614	23.058864763034535
50	22.53778674961497	21.44110322143625	30.190576650232025	25.83053337871675
51	21.386912725667358	22.27714664123398	27.654542329584707	28.681398303513955
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1086.0
1	1504.0
2	1922.0
3	2506.5
4	3091.0
5	2592.0
6	2093.0
7	2206.0
8	2319.0
9	2577.0
10	2835.0
11	3007.0
12	3179.0
13	3357.0
14	3535.0
15	3528.5
16	3522.0
17	3412.0
18	3302.0
19	3448.0
20	3594.0
21	3877.0
22	4160.0
23	4689.0
24	5218.0
25	5941.0
26	8081.5
27	9499.0
28	11371.0
29	13243.0
30	15895.0
31	18547.0
32	21072.0
33	23597.0
34	26698.0
35	29799.0
36	33297.0
37	36795.0
38	40289.5
39	43784.0
40	50451.5
41	57119.0
42	65140.5
43	73162.0
44	84436.0
45	95710.0
46	114349.5
47	132989.0
48	151317.0
49	169645.0
50	167427.5
51	165210.0
52	142569.5
53	119929.0
54	107355.0
55	94781.0
56	86210.0
57	77639.0
58	71600.0
59	65561.0
60	60890.5
61	56220.0
62	50512.0
63	44804.0
64	39899.0
65	34994.0
66	30154.5
67	25315.0
68	21314.0
69	17313.0
70	14306.5
71	11300.0
72	9842.5
73	8385.0
74	6465.0
75	4287.5
76	4030.0
77	2787.5
78	1545.0
79	1366.0
80	1187.0
81	759.5
82	332.0
83	204.5
84	77.0
85	72.5
86	68.0
87	37.0
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1483655.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.17273700590629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.78097787933967	17.74013708039109
2	13.585309605462648	7.1112947089517276
3	5.715909431699976	4.488029829163882
4	2.8793181727740214	3.014385491693644
5	1.6606553678367164	2.1731948099918474
6	1.0794006734108101	1.6950521969507564
7	0.755721333945378	1.384550700617354
8	0.5647227112653224	1.1824271202567707
9	0.39644693602154235	0.9338491253960265
>10	3.9528291867082794	25.406405333264587
>50	1.4949947283123872	26.343598259988532
>100	0.12528417926300414	5.092614889287931
>500	0.004941603356029846	0.9110529636310911
>1k	0.0026161429531922718	0.9743511737138525
>5k	8.720476510640905E-4	1.5490563167008473
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	7566	0.509956829586393	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	7346	0.4951285844755014	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	5449	0.3672686709511308	No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	2056	0.13857669067269682	No Hit
ACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	1748	0.11781714751744846	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	1583	0.1066959636842797	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.740111414041674E-5	0.0	0.0	0.5332102139648368	0.0
2	6.740111414041674E-5	0.0	0.0	1.7052481877525436	0.0
3	6.740111414041674E-5	0.0	0.0	2.3390882651290226	0.0
4	6.740111414041674E-5	0.0	0.0	3.0599431808607798	0.0
5	6.740111414041674E-5	0.0	0.0	4.735197872820838	0.0
6	6.740111414041674E-5	0.0	0.0	5.847720662822557	0.0
7	6.740111414041674E-5	0.0	0.0	6.875655054578052	0.0
8	6.740111414041674E-5	0.0	0.0	8.57672437325389	0.0
9	6.740111414041674E-5	0.0	0.0	9.163248868503796	0.0
10	6.740111414041674E-5	0.0	0.0	10.371818246155609	0.0
11	6.740111414041674E-5	0.0	0.0	12.26585695461546	0.0
12	6.740111414041674E-5	0.0	0.0	13.822418284574244	0.0
13	6.740111414041674E-5	0.0	0.0	14.439542885643899	0.0
14	6.740111414041674E-5	0.0	0.0	14.69175785475734	0.0
15	1.3480222828083347E-4	0.0	0.0	15.166598703876575	0.0
16	1.3480222828083347E-4	0.0	0.0	16.055754201616953	0.0
17	1.3480222828083347E-4	0.0	0.0	17.11449090253462	0.0
18	1.3480222828083347E-4	0.0	0.0	18.356356430571797	0.0
19	1.3480222828083347E-4	0.0	0.0	19.129312407534098	0.0
20	1.3480222828083347E-4	0.0	0.0	19.84295540405283	0.0
21	1.3480222828083347E-4	0.0	0.0	20.618337821124182	0.0
22	1.3480222828083347E-4	0.0	0.0	21.38772153903704	0.0
23	1.3480222828083347E-4	0.0	0.0	22.101229733327493	0.0
24	1.3480222828083347E-4	0.0	0.0	22.68451897509866	0.0
25	1.3480222828083347E-4	0.0	0.0	23.195082414712314	0.0
26	1.3480222828083347E-4	0.0	0.0	23.700186364080597	0.0
27	1.3480222828083347E-4	0.0	0.0	24.23919307386151	0.0
28	1.3480222828083347E-4	0.0	0.0	24.738635329641998	0.0
29	1.3480222828083347E-4	0.0	0.0	25.312286212091085	0.0
30	1.3480222828083347E-4	0.0	0.0	25.933657083351587	0.0
31	1.3480222828083347E-4	0.0	0.0	26.44361391293798	0.0
32	1.3480222828083347E-4	0.0	0.0	26.98066599040882	0.0
33	1.3480222828083347E-4	0.0	0.0	27.536051170925855	0.0
34	1.3480222828083347E-4	0.0	0.0	28.109836855603223	0.0
35	1.3480222828083347E-4	0.0	0.0	28.733229760287937	0.0
36	1.3480222828083347E-4	0.0	0.0	29.30014053132298	0.0
37	1.3480222828083347E-4	0.0	0.0	29.885991015431486	0.0
38	2.022033424212502E-4	0.0	0.0	30.55002679194287	0.0
39	2.022033424212502E-4	0.0	0.0	31.522624868989084	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTAT	60	0.0	45.000004	42
CCTTACG	35	1.2122473E-7	45.0	1
CGTATCG	25	3.8913568E-5	45.0	29
TTTCGAT	20	7.033951E-4	45.0	39
TAGCGCG	25	3.8913568E-5	45.0	1
CCGTACA	25	3.8913568E-5	45.0	20
CGTAGTC	25	3.8913568E-5	45.0	30
CTCGACC	25	3.8913568E-5	45.0	28
ACCGTAC	25	3.8913568E-5	45.0	19
AGTACGC	20	7.033951E-4	45.0	30
CCCGAAT	20	7.033951E-4	45.0	36
CCCCGAT	65	0.0	41.53846	40
GTAACGG	120	0.0	41.250004	2
CGTAAGG	230	0.0	41.086956	2
CTATCGG	55	6.184564E-11	40.90909	2
TCGTTAG	45	1.9290383E-8	40.0	1
CTTACGG	190	0.0	39.078945	2
ACGGGTA	245	0.0	38.57143	5
CATACGG	175	0.0	38.57143	2
ACGATTT	35	6.2495183E-6	38.571426	28
>>END_MODULE