FastQCFastQC Report
Sat 14 Jan 2017
SRR2935070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064666
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT90520.8502196933122689TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC82390.7738577168802234TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC79200.7438952685630986TruSeq Adapter, Index 16 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC29230.27454619570832545TruSeq Adapter, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC22870.21480915141462203TruSeq Adapter, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG20870.19602391735999836TruSeq Adapter, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCTT20130.18907338075978758TruSeq Adapter, Index 16 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC19430.18249854884066927No Hit
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG17780.16700073074560473TruSeq Adapter, Index 16 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG16500.15497818095064556TruSeq Adapter, Index 27 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG15680.14727623498824982TruSeq Adapter, Index 16 (95% over 23bp)
AAAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC15340.1440827451989638TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14790.13891680583394228No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC13530.12708210837952935No Hit
GGCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT12330.11581096794675513TruSeq Adapter, Index 27 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG11690.10979969304927555TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC302.1653505E-645.00000443
TTCGTTA302.1653505E-645.00000442
CTACGCG351.2118107E-745.0000041
TATACGG700.045.0000042
GCTAACG302.1653505E-645.0000041
TGTTTCG207.033053E-445.01
GTACCAT253.8906117E-545.016
TACCCGT207.033053E-445.044
GGTCGAC207.033053E-445.027
CAGCACG551.8189894E-1245.01
ATTCGTG207.033053E-445.020
CCTCGTC207.033053E-445.036
ATCGTCA207.033053E-445.010
CACTCGC207.033053E-445.022
GGTATAC207.033053E-445.08
TCCGTCG253.8906117E-545.013
TTACGAC207.033053E-445.013
TAGGCTA253.8906117E-545.039
ACCGTCC207.033053E-445.021
ACGTACA253.8906117E-545.01
Produced by FastQC (version 0.11.5)