##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935070.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1064666
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73192156037668	33.0	31.0	34.0	30.0	34.0
2	32.04591674759972	33.0	31.0	34.0	30.0	34.0
3	32.114706396184346	33.0	31.0	34.0	30.0	34.0
4	35.714688925916676	37.0	35.0	37.0	33.0	37.0
5	30.24382388467369	37.0	35.0	37.0	0.0	37.0
6	32.704124110284354	37.0	35.0	37.0	17.0	37.0
7	34.870248509861305	37.0	35.0	37.0	32.0	37.0
8	35.45996678770619	37.0	35.0	37.0	33.0	37.0
9	37.26881482079826	39.0	37.0	39.0	34.0	39.0
10	37.2306901882844	39.0	37.0	39.0	34.0	39.0
11	37.13842932901022	39.0	37.0	39.0	34.0	39.0
12	37.02358767914069	39.0	37.0	39.0	33.0	39.0
13	36.97498370380946	39.0	37.0	39.0	33.0	39.0
14	38.07903793302312	40.0	37.0	41.0	33.0	41.0
15	38.18505146214869	40.0	37.0	41.0	33.0	41.0
16	38.24368299541828	40.0	37.0	41.0	34.0	41.0
17	38.23627597763054	40.0	37.0	41.0	34.0	41.0
18	38.177579635303466	40.0	37.0	41.0	34.0	41.0
19	38.16599665998538	40.0	37.0	41.0	34.0	41.0
20	38.05140861077559	40.0	37.0	41.0	34.0	41.0
21	37.97075420836206	40.0	36.0	41.0	34.0	41.0
22	37.841483620215165	40.0	36.0	41.0	33.0	41.0
23	37.62099381402243	39.0	36.0	41.0	33.0	41.0
24	37.53442957697531	39.0	35.0	41.0	33.0	41.0
25	37.264642620314724	39.0	35.0	41.0	32.0	41.0
26	37.01153131592443	39.0	35.0	41.0	31.0	41.0
27	36.92779425660254	39.0	35.0	41.0	31.0	41.0
28	36.70312943214116	39.0	35.0	40.0	31.0	41.0
29	36.66309809837076	39.0	35.0	40.0	31.0	41.0
30	36.53315875589152	38.0	35.0	40.0	31.0	41.0
31	32.017185671374875	37.0	30.0	40.0	9.0	41.0
32	32.92229394007135	37.0	30.0	40.0	16.0	41.0
33	34.65674962852199	37.0	31.0	40.0	25.0	41.0
34	35.58853011179093	38.0	34.0	40.0	29.0	41.0
35	35.88799773825782	38.0	35.0	40.0	30.0	41.0
36	36.112232380859346	38.0	35.0	40.0	30.0	41.0
37	36.222461316506774	38.0	35.0	40.0	30.0	41.0
38	36.257708990425165	38.0	35.0	40.0	30.0	41.0
39	36.289349899405074	39.0	35.0	40.0	30.0	41.0
40	36.30240281928793	39.0	35.0	40.0	30.0	41.0
41	36.15318888740694	39.0	35.0	40.0	30.0	41.0
42	36.09162122205461	39.0	35.0	40.0	30.0	41.0
43	36.01084095857292	38.0	35.0	40.0	30.0	41.0
44	35.986360041552935	38.0	35.0	40.0	30.0	41.0
45	36.04903791423789	38.0	35.0	40.0	30.0	41.0
46	35.97728865202796	38.0	35.0	40.0	30.0	41.0
47	35.93650872668048	38.0	35.0	40.0	30.0	41.0
48	36.02992957415753	38.0	35.0	40.0	30.0	41.0
49	36.04506014092683	38.0	35.0	40.0	30.0	41.0
50	35.885124536709164	38.0	35.0	40.0	29.0	41.0
51	35.18856524017861	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	47.0
11	46.0
12	51.0
13	41.0
14	44.0
15	78.0
16	88.0
17	172.0
18	353.0
19	615.0
20	983.0
21	1517.0
22	2537.0
23	4279.0
24	5639.0
25	7178.0
26	8223.0
27	10140.0
28	12561.0
29	16179.0
30	21360.0
31	28795.0
32	40852.0
33	52617.0
34	82144.0
35	117058.0
36	126563.0
37	146334.0
38	212235.0
39	165787.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.771096287474194	21.34913672456902	24.84628982234804	13.033477165608746
2	31.22490997176579	24.482607691050525	25.95518218859248	18.3373001485912
3	29.47299904383159	24.025375094160985	28.129385178074628	18.3722406839328
4	26.85461919512786	26.604024172839182	27.118269955084507	19.42308667694845
5	21.00198559923957	38.371658341677104	22.35997016904832	18.266385890034996
6	25.523403583846953	31.49025140278735	26.391187471000293	16.5951575423654
7	73.05079715140711	7.349347119190432	12.005267379628917	7.594588349773544
8	71.30827884050022	5.870855272921273	12.90977639935905	9.911089487219465
9	65.24008468383512	7.96043078298734	16.095376390342135	10.704108142835405
10	37.18687362985199	23.66770423776095	21.768329222497947	17.37709290988911
11	24.93627109346969	25.23213852983001	26.98527049797777	22.84631987872253
12	22.081948705039892	21.035517242027076	34.4254442238223	22.45708982911073
13	22.11050226080292	24.216514850666783	34.551680996669376	19.121301891860924
14	16.545188819780098	29.237713987297425	31.651804415657114	22.56529277726536
15	14.405832439469279	26.068269297601315	37.724131323814234	21.80176693911518
16	15.881130795949153	28.514012845343046	31.68355146120943	23.92130489749837
17	16.394155537980925	27.263104109645653	28.08401883783271	28.258721514540706
18	18.592215774712447	25.23589557664094	32.62140427138652	23.550484377260098
19	19.61187827919742	26.868238489817465	28.994257353949504	24.525625877035615
20	19.479912009963687	27.754337980174064	30.18298696492609	22.582763044936158
21	19.577970931728824	28.003711962249195	31.46705163872989	20.95126546729209
22	17.32740596581463	27.509660306612588	28.023624310347095	27.139309417225682
23	16.817950418253236	27.26338588815647	29.03906013716978	26.879603556420513
24	20.759374301424106	24.666702984785836	28.85393165556146	25.71999105822859
25	17.927218489178767	27.481200677019835	26.822496444894455	27.769084388906943
26	15.906396935752621	27.097230492943325	29.900550970914825	27.09582160038923
27	19.044282432236965	25.740279111007585	28.683737435026575	26.531701021728882
28	15.798663618449355	24.31795511456175	32.3541843169595	27.5291969500294
29	16.11726118801577	22.071804678650395	33.046232339531834	28.764701793802
30	19.761126963761406	23.044598024169083	30.018522240777855	27.175752771291656
31	19.949730713669826	26.254430967082637	27.06388670249637	26.73195161675117
32	16.518044156571168	24.63937046923636	29.72782074378256	29.114764630409912
33	17.57461964597348	21.582355405357173	29.34666834481424	31.49635660385511
34	16.307555608989112	20.243249995773322	32.75881825849609	30.690376136741477
35	15.32264578750519	20.128753994210392	32.473564479376634	32.075035738907786
36	17.17430630826945	19.721677972246695	34.04926991187847	29.054745807605393
37	15.988770187082146	21.036926134581176	33.77059096467813	29.203712713658557
38	15.991494046020065	22.0976343754755	31.717740587188846	30.193130991315588
39	19.380444195644454	19.251201785348645	31.619869517764254	29.748484501242643
40	20.24954304918162	18.89118277469178	33.51285755344869	27.34641662267791
41	16.565007241707725	20.425372839932898	30.619649730525815	32.389970187833555
42	20.18304332062825	18.774150766531474	32.346576297167374	28.696229615672898
43	20.415886296735312	18.28301082217334	32.21864885325539	29.082454027835958
44	18.04143271223088	19.003330621997886	31.128259942554752	31.826976723216482
45	18.842341166149758	17.375120460313376	30.79444633340409	32.98809204013277
46	21.98924357498032	18.063599288415332	32.898486473692216	27.048670662912123
47	15.831631704215217	18.7391163050196	36.18120612473771	29.248045866027468
48	16.356397217531132	17.296879960475867	34.76958971170301	31.57713311028999
49	19.983262356457328	15.632508223236208	36.24225813541524	28.141971284891227
50	18.285171124089622	15.349696524543848	35.81047953066972	30.55465282069682
51	17.382070996913587	15.684637247737786	31.262386513704765	35.67090524164386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1836.0
1	1917.0
2	1998.0
3	2293.0
4	2588.0
5	2244.5
6	1901.0
7	2058.0
8	2215.0
9	2477.0
10	2739.0
11	2864.0
12	2989.0
13	3208.0
14	3427.0
15	3516.0
16	3605.0
17	3624.5
18	3644.0
19	3818.5
20	3993.0
21	3858.0
22	3723.0
23	4207.5
24	4692.0
25	4621.5
26	5119.0
27	5687.0
28	6496.5
29	7306.0
30	8403.5
31	9501.0
32	11248.5
33	12996.0
34	15473.0
35	17950.0
36	19266.0
37	20582.0
38	23272.0
39	25962.0
40	30753.0
41	35544.0
42	45230.0
43	54916.0
44	67718.0
45	80520.0
46	101865.5
47	123211.0
48	145698.5
49	168186.0
50	160343.5
51	152501.0
52	121738.0
53	90975.0
54	74321.0
55	57667.0
56	49037.0
57	40407.0
58	36029.5
59	31652.0
60	28300.5
61	24949.0
62	21929.0
63	18909.0
64	16497.0
65	14085.0
66	11733.5
67	9382.0
68	7963.5
69	6545.0
70	5559.5
71	4574.0
72	3640.5
73	2707.0
74	2259.0
75	1519.5
76	1228.0
77	894.5
78	561.0
79	381.5
80	202.0
81	175.0
82	148.0
83	111.0
84	74.0
85	49.0
86	24.0
87	13.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1064666.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.560864614481105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.60145934571794	20.659590468058344
2	12.966833695421851	7.925552980885577
3	5.96307955278006	5.467106006936758
4	3.2933809390416817	4.025942760078619
5	2.0081322589897783	3.068512904747936
6	1.311853716070493	2.4054830286500564
7	0.9092884161585588	1.9452048127216368
8	0.6761025794464127	1.652982351677062
9	0.5187291207766406	1.4267529388479356
>10	4.092156407414747	28.506031973106072
>50	0.5591901031756786	10.88553602200859
>100	0.0867174275225338	5.383399523437818
>500	0.007570569069423564	1.5760036190314015
>1k	0.0044735180864775605	2.4205842318994906
>5k	0.001032350327648668	2.6513163779127624
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	9052	0.8502196933122689	TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	8239	0.7738577168802234	TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	7920	0.7438952685630986	TruSeq Adapter, Index 16 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2923	0.27454619570832545	TruSeq Adapter, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2287	0.21480915141462203	TruSeq Adapter, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	2087	0.19602391735999836	TruSeq Adapter, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCTT	2013	0.18907338075978758	TruSeq Adapter, Index 16 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCC	1943	0.18249854884066927	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1778	0.16700073074560473	TruSeq Adapter, Index 16 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1650	0.15497818095064556	TruSeq Adapter, Index 27 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1568	0.14727623498824982	TruSeq Adapter, Index 16 (95% over 23bp)
AAAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	1534	0.1440827451989638	TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1479	0.13891680583394228	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	1353	0.12708210837952935	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	1233	0.11581096794675513	TruSeq Adapter, Index 27 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	1169	0.10979969304927555	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8785234054623703E-4	0.0	0.0	0.9127745227141657	0.0
2	1.8785234054623703E-4	0.0	0.0	3.0807783849582875	0.0
3	1.8785234054623703E-4	0.0	0.0	4.360992085780893	0.0
4	1.8785234054623703E-4	0.0	0.0	5.826616046722634	0.0
5	1.8785234054623703E-4	0.0	0.0	9.192460358459837	0.0
6	1.8785234054623703E-4	0.0	0.0	11.462749820131384	0.0
7	1.8785234054623703E-4	0.0	0.0	13.412657115001325	0.0
8	2.8177851081935556E-4	0.0	0.0	16.55364217510468	0.0
9	2.8177851081935556E-4	0.0	0.0	17.671551453695336	0.0
10	2.8177851081935556E-4	0.0	0.0	19.933669338553123	0.0
11	2.8177851081935556E-4	0.0	0.0	23.604491925167142	0.0
12	2.8177851081935556E-4	0.0	0.0	26.52803790108823	0.0
13	2.8177851081935556E-4	0.0	0.0	27.78711821359938	0.0
14	2.8177851081935556E-4	0.0	0.0	28.3328292628862	0.0
15	2.8177851081935556E-4	0.0	0.0	29.139561139362016	0.0
16	2.8177851081935556E-4	0.0	0.0	30.678917143968157	0.0
17	3.7570468109247407E-4	0.0	0.0	32.42218686423723	0.0
18	3.7570468109247407E-4	0.0	0.0	34.49278928790813	0.0
19	3.7570468109247407E-4	0.0	0.0	35.70763037422065	0.0
20	3.7570468109247407E-4	0.0	0.0	36.91617840712486	0.0
21	3.7570468109247407E-4	0.0	0.0	38.08640456255765	0.0
22	3.7570468109247407E-4	0.0	0.0	39.181771560282755	0.0
23	3.7570468109247407E-4	0.0	0.0	40.21993752031153	0.0
24	3.7570468109247407E-4	0.0	0.0	41.03502882594166	0.0
25	3.7570468109247407E-4	0.0	0.0	41.74651956576053	0.0
26	3.7570468109247407E-4	0.0	0.0	42.395173697666685	0.0
27	3.7570468109247407E-4	0.0	0.0	43.11793557791833	0.0
28	3.7570468109247407E-4	0.0	0.0	43.77898796430054	0.0
29	3.7570468109247407E-4	0.0	0.0	44.497429240719626	0.0
30	3.7570468109247407E-4	0.0	0.0	45.173321962004984	0.0
31	4.6963085136559257E-4	0.0	0.0	45.84254592520095	0.0
32	4.6963085136559257E-4	0.0	0.0	46.53778743756258	0.0
33	5.635570216387111E-4	0.0	0.0	47.21321052799657	0.0
34	5.635570216387111E-4	0.0	0.0	47.921319925685616	0.0
35	5.635570216387111E-4	0.0	0.0	48.61120764634167	0.0
36	5.635570216387111E-4	0.0	0.0	49.297432246357076	0.0
37	5.635570216387111E-4	0.0	0.0	49.9685347329585	0.0
38	5.635570216387111E-4	0.0	0.0	50.58929279229355	0.0
39	5.635570216387111E-4	0.0	0.0	51.289888096360734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	30	2.1653505E-6	45.000004	43
TTCGTTA	30	2.1653505E-6	45.000004	42
CTACGCG	35	1.2118107E-7	45.000004	1
TATACGG	70	0.0	45.000004	2
GCTAACG	30	2.1653505E-6	45.000004	1
TGTTTCG	20	7.033053E-4	45.0	1
GTACCAT	25	3.8906117E-5	45.0	16
TACCCGT	20	7.033053E-4	45.0	44
GGTCGAC	20	7.033053E-4	45.0	27
CAGCACG	55	1.8189894E-12	45.0	1
ATTCGTG	20	7.033053E-4	45.0	20
CCTCGTC	20	7.033053E-4	45.0	36
ATCGTCA	20	7.033053E-4	45.0	10
CACTCGC	20	7.033053E-4	45.0	22
GGTATAC	20	7.033053E-4	45.0	8
TCCGTCG	25	3.8906117E-5	45.0	13
TTACGAC	20	7.033053E-4	45.0	13
TAGGCTA	25	3.8906117E-5	45.0	39
ACCGTCC	20	7.033053E-4	45.0	21
ACGTACA	25	3.8906117E-5	45.0	1
>>END_MODULE