FastQCFastQC Report
Sat 14 Jan 2017
SRR2935069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568213
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG7520.13234473692083779No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG6630.11668159651398331No Hit
GCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC6040.10629816635663034No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG253.8883063E-545.00000417
TTTCACG253.8883063E-545.0000041
GCCGTTG253.8883063E-545.0000041
GCCGATA207.030272E-445.09
TACGAAC207.030272E-445.021
ACTCGAC207.030272E-445.020
CGGTCTA207.030272E-445.031
TATCGCG207.030272E-445.01
ACGAACG207.030272E-445.022
TCGTAAG302.163557E-644.9999961
CCCGATT302.163557E-644.99999642
CCGCTTA403.4541154E-739.37531
TATACGG403.4541154E-739.3752
GTCAGCG750.039.035
TGGGCGA2500.038.76
CTACGGG1400.038.5714263
ACCCGAT356.2427134E-638.57142641
TCTACGG659.094947E-1238.0769272
GGGCGAT8250.037.9090927
TATTGCG301.1391957E-437.4999961
Produced by FastQC (version 0.11.5)