##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935069.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568213
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.632933424613654	34.0	31.0	34.0	31.0	34.0
2	32.99123040127558	34.0	33.0	34.0	31.0	34.0
3	33.01525484281423	34.0	33.0	34.0	31.0	34.0
4	36.35829697666192	37.0	37.0	37.0	35.0	37.0
5	30.823745672837475	37.0	35.0	37.0	0.0	37.0
6	33.292592742510294	37.0	35.0	37.0	17.0	37.0
7	35.56127895701084	37.0	35.0	37.0	32.0	37.0
8	36.02719578749518	37.0	35.0	37.0	35.0	37.0
9	37.954972871088835	39.0	39.0	39.0	35.0	39.0
10	37.94106259448481	39.0	38.0	39.0	35.0	39.0
11	37.87032151675516	39.0	38.0	39.0	35.0	39.0
12	37.6780115907239	39.0	37.0	39.0	35.0	39.0
13	37.60917648839432	39.0	37.0	39.0	35.0	39.0
14	38.84927307189381	40.0	38.0	41.0	35.0	41.0
15	38.9909769751836	40.0	38.0	41.0	35.0	41.0
16	39.1191683400415	40.0	39.0	41.0	35.0	41.0
17	39.13021349388345	40.0	38.0	41.0	35.0	41.0
18	39.07250450095299	40.0	38.0	41.0	35.0	41.0
19	39.0517288411212	40.0	38.0	41.0	35.0	41.0
20	38.95507670539041	40.0	38.0	41.0	35.0	41.0
21	38.89990725308995	40.0	38.0	41.0	35.0	41.0
22	38.89580139841925	40.0	38.0	41.0	35.0	41.0
23	38.85603462082001	40.0	38.0	41.0	35.0	41.0
24	38.86459479103787	40.0	38.0	41.0	35.0	41.0
25	38.82382134868438	40.0	38.0	41.0	35.0	41.0
26	38.78158014688154	40.0	38.0	41.0	35.0	41.0
27	38.71622437360638	40.0	38.0	41.0	35.0	41.0
28	38.688048319908205	40.0	38.0	41.0	35.0	41.0
29	38.67785495931983	40.0	38.0	41.0	35.0	41.0
30	38.58222004776378	40.0	38.0	41.0	35.0	41.0
31	33.96022618278709	40.0	35.0	41.0	9.0	41.0
32	34.95127355410735	40.0	35.0	41.0	16.0	41.0
33	36.923618431820465	40.0	35.0	41.0	30.0	41.0
34	37.67951102843476	40.0	36.0	41.0	33.0	41.0
35	37.76839671038854	40.0	37.0	41.0	34.0	41.0
36	37.95481799958818	40.0	37.0	41.0	34.0	41.0
37	38.05107239714685	40.0	37.0	41.0	34.0	41.0
38	38.006305733941325	40.0	37.0	41.0	34.0	41.0
39	38.00002815845466	40.0	36.0	41.0	34.0	41.0
40	37.96121348860375	40.0	36.0	41.0	34.0	41.0
41	37.912802065422646	40.0	36.0	41.0	34.0	41.0
42	37.856669945953364	40.0	36.0	41.0	34.0	41.0
43	37.834474044064464	40.0	36.0	41.0	34.0	41.0
44	37.78197436524683	40.0	35.0	41.0	34.0	41.0
45	37.74636975922761	40.0	35.0	41.0	34.0	41.0
46	37.68733907883136	40.0	35.0	41.0	34.0	41.0
47	37.5867342000271	40.0	35.0	41.0	34.0	41.0
48	37.54391750980706	40.0	35.0	41.0	34.0	41.0
49	37.489430900032204	39.0	35.0	41.0	34.0	41.0
50	37.224964933924426	39.0	35.0	41.0	34.0	41.0
51	36.72958908015128	39.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	1.0
11	5.0
12	2.0
13	4.0
14	4.0
15	10.0
16	14.0
17	24.0
18	49.0
19	78.0
20	151.0
21	258.0
22	468.0
23	823.0
24	1407.0
25	1865.0
26	2229.0
27	2510.0
28	2760.0
29	3216.0
30	4315.0
31	6624.0
32	11916.0
33	13830.0
34	32512.0
35	45129.0
36	61400.0
37	66630.0
38	99389.0
39	210294.0
40	292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47461075336186	22.44985595190536	26.751235892174236	10.324297402558548
2	33.43323718394334	24.460545605257185	24.674901841386944	17.431315369412527
3	32.50682402549748	23.966540716245493	25.696877755348783	17.82975750290824
4	29.623398267903056	26.262686703753698	25.420572918958207	18.693342109385036
5	23.70924283675312	39.25147787889401	20.342195620304356	16.697083664048517
6	28.242402056975113	33.69018308275242	22.91130262771179	15.156112232560679
7	81.82952519565725	6.529945636583465	7.39458618511016	4.245942982649113
8	83.06409744233237	5.565694554682839	6.909908784205923	4.460299218778873
9	76.43366132066673	7.797956048172076	9.387148833272029	6.3812337978891716
10	41.89098102296146	25.510679973883033	16.74072926877773	15.857609734377778
11	32.21063228050045	24.879578608725954	23.52533292972882	19.384456181044783
12	27.401872185254472	23.53571635988617	28.730423274370704	20.331988180488654
13	25.858436888983533	22.92098209650254	31.321705064826045	19.89887594968788
14	21.448998878941524	26.017004186810226	31.504911010483745	21.029085923764505
15	20.721982777585165	24.848604308595547	33.52580810365127	20.903604810168016
16	24.472688938831038	25.798776163164167	29.426289085255004	20.302245812749796
17	25.29297992126192	24.76782474177817	27.50729039990285	22.431904937057055
18	25.528454998389687	24.284907244290434	28.97223400379787	21.214403753522006
19	25.305651225860725	26.459971876743403	27.225881843604423	21.00849505379145
20	24.793519331659077	28.33127717950839	27.21866623959677	19.65653724923576
21	23.82082071335925	26.641593909326254	29.502844883872775	20.03474049344172
22	23.382252781967324	24.58884256432007	29.153328065355772	22.87557658835683
23	22.24588314593295	26.013836360660527	29.554410053976238	22.185870439430282
24	22.317511214984524	26.157092498763667	28.66055510873563	22.86484117751618
25	23.22174959038248	27.07558609183528	27.025956815489966	22.676707502292274
26	21.40975303275356	27.607252913960085	27.503946583411505	23.479047469874853
27	22.10773072773766	25.881491623739688	29.24783487882185	22.762942769700796
28	20.608644997562532	26.625578788236105	28.751190134685405	24.014586079515958
29	22.05528560592595	26.712518017011227	28.3685871319382	22.86360924512463
30	23.461800416393146	25.91510577899485	28.54387351222165	22.079220292390353
31	24.999076050706336	27.31387701442945	26.013132399293927	21.673914535570287
32	24.002794726625403	27.03405237120587	26.33924250237147	22.62391039979726
33	24.15485038181105	25.936400610334502	26.99656642843441	22.91218257942004
34	21.88791879101675	26.529136081011874	28.718983902163448	22.863961225807927
35	22.60208759743265	25.85544505317548	28.547745299737947	22.994722049653916
36	23.485031141490957	27.140174547220848	28.357323750072595	21.0174705612156
37	22.218252662293896	28.11868084679513	27.53826470003326	22.124801790877715
38	23.43645780719554	27.08068981174313	26.216049263216433	23.26680311784489
39	23.524276987678917	24.66469440157124	27.093713097025233	24.717315513724607
40	23.96566076453724	24.13830728969594	29.09577922363621	22.800252722130608
41	21.757157967170762	24.834877061946838	28.616733513664773	24.791231457217627
42	21.730231444898305	25.41846103485841	30.294449440614702	22.55685807962859
43	21.599998592077267	24.773984403735923	30.780006793227187	22.846010210959623
44	22.584312572926	25.0390258582609	27.80154625114174	24.57511531767137
45	22.51198054250783	23.692523754296364	27.515385955618754	26.28010974757705
46	22.45689556557136	25.016851075212994	28.11428108825388	24.411972270961773
47	20.6616180903992	25.60043504812456	30.419930554211184	23.318016307265058
48	20.969777178628437	25.900498580637894	29.286200773301562	23.843523467432107
49	22.433840830815203	24.744418026338717	29.759790782681844	23.061950360164236
50	21.095082301883274	24.597818071744225	29.314359227965568	24.992740398406934
51	21.13397616738793	24.58039502792087	27.524889434067855	26.760739370623337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	291.0
1	566.5
2	842.0
3	1195.5
4	1549.0
5	1239.5
6	930.0
7	928.5
8	927.0
9	1012.0
10	1097.0
11	1136.0
12	1175.0
13	1231.0
14	1287.0
15	1335.0
16	1383.0
17	1387.0
18	1391.0
19	1554.5
20	1718.0
21	1861.5
22	2005.0
23	2319.5
24	2634.0
25	3617.0
26	5419.5
27	6239.0
28	7380.5
29	8522.0
30	9302.0
31	10082.0
32	11241.5
33	12401.0
34	13829.0
35	15257.0
36	16791.0
37	18325.0
38	19335.5
39	20346.0
40	22782.5
41	25219.0
42	26901.5
43	28584.0
44	32199.0
45	35814.0
46	40146.0
47	44478.0
48	48924.5
49	53371.0
50	52345.0
51	51319.0
52	46448.5
53	41578.0
54	37642.5
55	33707.0
56	31783.0
57	29859.0
58	28251.0
59	26643.0
60	24443.5
61	22244.0
62	20227.0
63	18210.0
64	16087.0
65	13964.0
66	11764.5
67	9565.0
68	8353.5
69	7142.0
70	6008.0
71	4874.0
72	4161.0
73	3448.0
74	2950.0
75	1956.0
76	1460.0
77	1101.0
78	742.0
79	541.0
80	340.0
81	213.0
82	86.0
83	72.5
84	59.0
85	44.5
86	30.0
87	25.0
88	20.0
89	11.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	568213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.35414162944203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54947528270957	25.647656163104433
2	10.068721060454413	7.320794229182109
3	3.7308120367747457	4.068914075332086
4	2.159205357121373	3.139842294393614
5	1.6055969478307488	2.9185049420619444
6	1.3590881534718324	2.9645089930927107
7	1.2070000459328867	3.0715615431611045
8	1.0735099031723643	3.1221224848429383
9	0.991266322863267	3.243297266448068
>10	7.174409204939172	40.88977279652643
>50	0.053579304753417635	1.3161763094346068
>100	0.02405601437908315	1.5858910862243965
>500	0.0032803655971477023	0.7109578161955967
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	752	0.13234473692083779	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	663	0.11668159651398331	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	604	0.10629816635663034	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09327488107452662	0.0
2	0.0	0.0	0.0	0.3555004901331015	0.0
3	0.0	0.0	0.0	0.5893916541860182	0.0
4	0.0	0.0	0.0	0.9721706472748776	0.0
5	0.0	0.0	0.0	1.8431468481009763	0.0
6	0.0	0.0	0.0	2.5803703892730367	0.0
7	0.0	0.0	0.0	3.1905289037737607	0.0
8	0.0	0.0	0.0	4.2235922092595555	0.0
9	0.0	0.0	0.0	4.654768546302179	0.0
10	0.0	0.0	0.0	5.5398239744602815	0.0
11	0.0	0.0	0.0	7.0037116363053995	0.0
12	0.0	0.0	0.0	8.154336490013428	0.0
13	0.0	0.0	0.0	8.58164103953975	0.0
14	1.7599034165005024E-4	0.0	0.0	8.769422734080354	0.0
15	1.7599034165005024E-4	0.0	0.0	9.078989745042792	0.0
16	1.7599034165005024E-4	0.0	0.0	9.693372027743118	0.0
17	1.7599034165005024E-4	0.0	0.0	10.395749481268467	0.0
18	1.7599034165005024E-4	0.0	0.0	11.229063748981456	0.0
19	1.7599034165005024E-4	0.0	0.0	11.777977624587963	0.0
20	1.7599034165005024E-4	0.0	0.0	12.292221402889409	0.0
21	1.7599034165005024E-4	0.0	0.0	12.88988460313298	0.0
22	1.7599034165005024E-4	0.0	0.0	13.504442876174956	0.0
23	1.7599034165005024E-4	0.0	0.0	14.097354337193975	0.0
24	1.7599034165005024E-4	0.0	0.0	14.60385454046282	0.0
25	1.7599034165005024E-4	0.0	0.0	15.044182375271244	0.0
26	1.7599034165005024E-4	0.0	0.0	15.465327262839816	0.0
27	1.7599034165005024E-4	0.0	0.0	15.89949543569049	0.0
28	1.7599034165005024E-4	0.0	0.0	16.329615830683213	0.0
29	1.7599034165005024E-4	0.0	0.0	16.8046137627967	0.0
30	1.7599034165005024E-4	0.0	0.0	17.33610459457985	0.0
31	1.7599034165005024E-4	0.0	0.0	17.817966150017686	0.0
32	1.7599034165005024E-4	0.0	0.0	18.332385918660783	0.0
33	1.7599034165005024E-4	0.0	0.0	18.899602789798895	0.0
34	1.7599034165005024E-4	0.0	0.0	19.47861101382756	0.0
35	1.7599034165005024E-4	0.0	0.0	20.078914069195882	0.0
36	1.7599034165005024E-4	0.0	0.0	20.62642002206919	0.0
37	1.7599034165005024E-4	0.0	0.0	21.16354254478514	0.0
38	1.7599034165005024E-4	0.0	0.0	21.77546096270237	0.0
39	1.7599034165005024E-4	0.0	0.0	22.605255423582353	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCG	25	3.8883063E-5	45.000004	17
TTTCACG	25	3.8883063E-5	45.000004	1
GCCGTTG	25	3.8883063E-5	45.000004	1
GCCGATA	20	7.030272E-4	45.0	9
TACGAAC	20	7.030272E-4	45.0	21
ACTCGAC	20	7.030272E-4	45.0	20
CGGTCTA	20	7.030272E-4	45.0	31
TATCGCG	20	7.030272E-4	45.0	1
ACGAACG	20	7.030272E-4	45.0	22
TCGTAAG	30	2.163557E-6	44.999996	1
CCCGATT	30	2.163557E-6	44.999996	42
CCGCTTA	40	3.4541154E-7	39.375	31
TATACGG	40	3.4541154E-7	39.375	2
GTCAGCG	75	0.0	39.0	35
TGGGCGA	250	0.0	38.7	6
CTACGGG	140	0.0	38.571426	3
ACCCGAT	35	6.2427134E-6	38.571426	41
TCTACGG	65	9.094947E-12	38.076927	2
GGGCGAT	825	0.0	37.909092	7
TATTGCG	30	1.1391957E-4	37.499996	1
>>END_MODULE