FastQCFastQC Report
Sat 14 Jan 2017
SRR2935068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694442
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC78320.4622170602475623TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT74760.4412071938726731TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC60030.35427592092264TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC23160.13668216439394207No Hit
TCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC19730.11643951224060782TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACG207.034234E-445.00000427
AATTCCG207.034234E-445.00000413
GCGTAGT253.891592E-545.014
TTGCGAA302.1661144E-644.99999613
GCGTAAG2200.042.9545441
CTAACGG1500.042.02
CGTGCAA1050.040.71428725
CGTATGG1900.040.2631572
ATATACG451.9292202E-840.01
CGTAAGG4000.039.93752
TACGGGA5300.039.9056634
CACAACG1250.039.612
CGTCTAC356.250044E-638.57142641
ATATGCG356.250044E-638.5714261
GCGCATT356.250044E-638.57142626
TCATCGC356.250044E-638.57142612
AACTCGC356.250044E-638.57142619
TAATCCG356.250044E-638.5714261
TACGGGC3950.037.5949364
GCTAACG900.037.51
Produced by FastQC (version 0.11.5)