##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935068.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1694442
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99418451620061	33.0	31.0	34.0	30.0	34.0
2	32.317718753430334	34.0	31.0	34.0	30.0	34.0
3	32.37923930119768	34.0	31.0	34.0	30.0	34.0
4	35.894159847312565	37.0	35.0	37.0	35.0	37.0
5	30.381830124607394	37.0	35.0	37.0	0.0	37.0
6	32.850390866137644	37.0	35.0	37.0	17.0	37.0
7	35.06684973578322	37.0	35.0	37.0	32.0	37.0
8	35.65188776010037	37.0	35.0	37.0	35.0	37.0
9	37.564749339310524	39.0	37.0	39.0	35.0	39.0
10	37.40714583325956	39.0	37.0	39.0	35.0	39.0
11	37.273047410297906	39.0	37.0	39.0	34.0	39.0
12	37.11828731818498	39.0	37.0	39.0	34.0	39.0
13	37.08666333813728	39.0	37.0	39.0	33.0	39.0
14	38.284764541955404	40.0	38.0	41.0	34.0	41.0
15	38.39584535794084	40.0	38.0	41.0	34.0	41.0
16	38.46119017352025	40.0	38.0	41.0	34.0	41.0
17	38.44527165875256	40.0	38.0	41.0	34.0	41.0
18	38.38343596298959	40.0	37.0	41.0	34.0	41.0
19	38.370953387604885	40.0	37.0	41.0	34.0	41.0
20	38.292952488193755	40.0	37.0	41.0	34.0	41.0
21	38.23297404101173	40.0	37.0	41.0	34.0	41.0
22	38.21950352977559	40.0	37.0	41.0	34.0	41.0
23	38.14911103478313	40.0	37.0	41.0	34.0	41.0
24	38.130573368696005	40.0	37.0	41.0	34.0	41.0
25	38.08133946160447	40.0	37.0	41.0	34.0	41.0
26	38.0040803993291	40.0	36.0	41.0	34.0	41.0
27	37.95167848766733	40.0	36.0	41.0	34.0	41.0
28	37.91739699558911	40.0	36.0	41.0	34.0	41.0
29	37.90880537663727	40.0	36.0	41.0	34.0	41.0
30	37.73182203934983	40.0	36.0	41.0	33.0	41.0
31	33.157193341524824	38.0	33.0	41.0	9.0	41.0
32	34.03548719873563	38.0	33.0	41.0	16.0	41.0
33	35.95276380070843	38.0	34.0	41.0	29.0	41.0
34	36.84406134880982	38.0	35.0	41.0	31.0	41.0
35	36.97464770113111	39.0	35.0	41.0	32.0	41.0
36	37.09509502243216	39.0	35.0	41.0	32.0	41.0
37	37.2371848667585	39.0	35.0	41.0	33.0	41.0
38	37.19912395939194	39.0	35.0	41.0	32.0	41.0
39	37.23509037193365	39.0	35.0	41.0	33.0	41.0
40	37.21940556242114	39.0	35.0	41.0	33.0	41.0
41	37.1347664894992	39.0	35.0	41.0	32.0	41.0
42	37.03300791647044	39.0	35.0	41.0	32.0	41.0
43	37.02137635870688	39.0	35.0	41.0	32.0	41.0
44	36.962905192387815	39.0	35.0	41.0	32.0	41.0
45	36.93567321867612	39.0	35.0	41.0	32.0	41.0
46	36.851727589377504	39.0	35.0	41.0	32.0	41.0
47	36.73613791442847	39.0	35.0	41.0	32.0	41.0
48	36.72251218985365	39.0	35.0	41.0	32.0	41.0
49	36.68756794272097	39.0	35.0	41.0	31.0	41.0
50	36.49609901076579	39.0	35.0	40.0	31.0	41.0
51	35.79680803473946	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	7.0
11	9.0
12	18.0
13	15.0
14	32.0
15	54.0
16	86.0
17	162.0
18	312.0
19	552.0
20	968.0
21	1630.0
22	2616.0
23	4302.0
24	6107.0
25	7916.0
26	9836.0
27	11955.0
28	15056.0
29	19385.0
30	25941.0
31	35586.0
32	53790.0
33	63987.0
34	114845.0
35	163947.0
36	196818.0
37	199519.0
38	301908.0
39	456634.0
40	442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02392291975766	20.715610212683586	25.69311903269631	11.56734783486245
2	31.175631859927925	24.578238735819816	26.018594912071347	18.227534492180908
3	31.55345535580445	24.15084139793513	26.8346157614129	17.461087484847518
4	27.69247929406849	26.073893352501887	27.16628837103896	19.067338982390662
5	22.560288283694575	39.41852243983565	21.220968318773966	16.800220957695807
6	27.273993444449555	33.520415570435574	23.78216545623869	15.423425528876173
7	79.78750526722071	5.876447821760792	8.791979896626737	5.544067014391758
8	80.67080490214478	4.648846050794303	8.360451405241372	6.319897641819548
9	75.18793797604168	6.483078205096426	11.094507808470281	7.234476010391622
10	41.10196749136294	23.75690640340596	18.60659733410763	16.534528771123473
11	28.943392574074533	26.45962505650828	24.687950369502172	19.909031999915015
12	25.833106119890797	23.641647220736974	29.52016061924811	21.005086040124123
13	24.14423155233404	24.524238657918062	31.54401271923146	19.78751707051643
14	20.40636386491836	27.479724888783448	29.94236450701765	22.171546739280544
15	19.159935837284486	26.632779404665367	32.93792292683963	21.26936183121051
16	21.706555904539666	26.759664833614842	30.096869647943098	21.436909613902394
17	21.420916148206903	26.222791927962124	27.887823838172093	24.468468085658877
18	21.24616835512812	25.787840480819053	31.025198856024577	21.940792308028247
19	22.66279990699003	27.62874149720085	28.057791296485806	21.650667299323317
20	23.77030314404388	27.72806623065292	28.072899515002582	20.428731110300618
21	23.539961828141653	28.07868312990353	28.8667891848762	19.514565857078615
22	21.71139525578332	25.509046636001703	27.95681410163346	24.82274400658152
23	21.258384766194418	26.364077377685398	28.436145940669554	23.941391915450634
24	22.387724100323293	25.196790447828842	29.157386325409778	23.258099126438083
25	21.110312421434312	27.2791868945647	26.799796039050023	24.810704644950963
26	20.754915187418632	27.673771070358267	28.647365917511486	22.923947824711615
27	22.34440600504473	26.970766777499612	28.15156848095125	22.533258736504408
28	20.77120373550703	26.279211681485705	28.71676929632292	24.232815286684346
29	22.406727406426423	24.855321102758314	28.144899618871584	24.593051871943683
30	23.29527950794421	26.05400479922004	28.03695847954666	22.613757213289094
31	26.487303784962833	27.042353766018547	24.44350411521905	22.026838333799564
32	24.268166157354457	27.039934090396724	25.701263306740508	22.990636445508315
33	22.753803316962163	27.93686653187303	26.557946509824472	22.751383641340336
34	24.451589372784667	25.005990172575988	27.53573152695696	23.006688927682386
35	22.28084525761283	25.321079151720742	28.264585037434152	24.133490553232274
36	23.187456401576448	27.99995514747628	28.129673367397643	20.68291508354963
37	23.722794878786054	26.171447591596525	28.897654803174145	21.20810272644328
38	21.109309141298432	27.78944336837732	27.19544251145805	23.9058049788662
39	23.300413941580768	27.02411767413697	26.735645126832313	22.939823257449945
40	23.287843431642983	25.041045960853193	29.407439145158108	22.263671462345716
41	21.542667143519814	25.677007534043657	28.237909589115475	24.542415733321057
42	23.28005325647027	24.57694037329103	29.125104311625893	23.017902058612805
43	22.732852467065854	24.64740604871692	29.22962249519311	23.390118989024113
44	22.55149482838598	24.419956540265172	27.35266241039823	25.675886220950613
45	22.774577117422727	23.421633788586448	27.298072167710668	26.505716926280154
46	24.331549855350612	24.22821200135502	28.744270975341735	22.695967167952634
47	20.93143347485485	24.66941919522769	31.10475306915197	23.29439426076549
48	21.235073257154863	23.313810682218687	30.509866965053984	24.941249095572466
49	22.883757602797854	22.91686584728188	31.177107271892456	23.02226927802781
50	21.938077550013517	22.184530364568396	30.538194874772934	25.33919721064516
51	20.682974100028208	22.719573759385096	28.06198146646507	28.535470674121626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1406.0
1	1791.0
2	2176.0
3	2994.5
4	3813.0
5	3193.5
6	2574.0
7	2679.5
8	2785.0
9	3006.5
10	3228.0
11	3393.5
12	3559.0
13	3659.0
14	3759.0
15	3775.5
16	3792.0
17	3950.0
18	4108.0
19	4242.0
20	4376.0
21	4947.5
22	5519.0
23	6184.0
24	6849.0
25	7744.5
26	11400.0
27	14160.0
28	15906.5
29	17653.0
30	20681.0
31	23709.0
32	28074.0
33	32439.0
34	35668.0
35	38897.0
36	42822.0
37	46747.0
38	52717.0
39	58687.0
40	64706.5
41	70726.0
42	78968.0
43	87210.0
44	101013.0
45	114816.0
46	138241.5
47	161667.0
48	181045.5
49	200424.0
50	194513.0
51	188602.0
52	163871.0
53	139140.0
54	121630.5
55	104121.0
56	93217.0
57	82313.0
58	74849.5
59	67386.0
60	60371.5
61	53357.0
62	48549.0
63	43741.0
64	37587.0
65	31433.0
66	26249.5
67	21066.0
68	17590.0
69	14114.0
70	11900.0
71	9686.0
72	8178.5
73	6671.0
74	5528.0
75	3501.5
76	2618.0
77	1891.5
78	1165.0
79	885.5
80	606.0
81	420.0
82	234.0
83	150.5
84	67.0
85	38.0
86	9.0
87	7.0
88	5.0
89	4.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1694442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.97873599564538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.15706644652171	17.18676963460557
2	13.937065563179077	7.241346936396599
3	6.288969081362356	4.901384023484672
4	3.172045017695452	3.2962288032404965
5	1.8551646100789372	2.4097415816852554
6	1.1981007919176896	1.8675086501641993
7	0.7638425735141674	1.3890565191691233
8	0.5426710318934982	1.1278325976036516
9	0.4190359145395227	0.9797421056864474
>10	4.153897342092783	27.41167537267289
>50	1.3981333962186848	24.005275568741233
>100	0.10429452541855506	4.535921179799448
>500	0.0056237242759339095	1.0188954910503518
>1k	0.003323109799415492	1.2134040547788585
>5k	7.668714921728058E-4	1.415217480921205
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	7832	0.4622170602475623	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	7476	0.4412071938726731	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	6003	0.35427592092264	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	2316	0.13668216439394207	No Hit
TCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1973	0.11643951224060782	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46245312616188694	0.0
2	0.0	0.0	0.0	1.5433989478542198	0.0
3	0.0	0.0	0.0	2.1251243772286097	0.0
4	0.0	0.0	0.0	2.8612369145712866	0.0
5	0.0	0.0	0.0	4.60505582368709	0.0
6	0.0	0.0	0.0	5.795359180190292	0.0
7	0.0	0.0	0.0	6.821478693280738	0.0
8	0.0	0.0	0.0	8.697081399068248	0.0
9	0.0	0.0	0.0	9.281698635893115	0.0
10	0.0	0.0	0.0	10.675844909415607	0.0
11	0.0	0.0	0.0	12.736641324990764	0.0
12	0.0	0.0	0.0	14.41689948667467	0.0
13	0.0	0.0	0.0	15.049910235936078	0.0
14	0.0	0.0	0.0	15.315543406029832	0.0
15	0.0	0.0	0.0	15.790508025650922	0.0
16	5.901647858114943E-5	0.0	0.0	16.775847152041795	0.0
17	5.901647858114943E-5	0.0	0.0	17.8913176137041	0.0
18	1.1803295716229885E-4	0.0	0.0	19.15539156843374	0.0
19	1.1803295716229885E-4	0.0	0.0	19.98941244374254	0.0
20	1.1803295716229885E-4	0.0	0.0	20.69949871403093	0.0
21	1.1803295716229885E-4	0.0	0.0	21.536529429747375	0.0
22	1.7704943574344827E-4	0.0	0.0	22.395927390846072	0.0
23	1.7704943574344827E-4	0.0	0.0	23.221685959153515	0.0
24	1.7704943574344827E-4	0.0	0.0	23.914893516567695	0.0
25	1.7704943574344827E-4	0.0	0.0	24.5291370256403	0.0
26	1.7704943574344827E-4	0.0	0.0	25.08973455568264	0.0
27	1.7704943574344827E-4	0.0	0.0	25.6032959522958	0.0
28	1.7704943574344827E-4	0.0	0.0	26.138516396548244	0.0
29	1.7704943574344827E-4	0.0	0.0	26.744556615098066	0.0
30	1.7704943574344827E-4	0.0	0.0	27.36375750837149	0.0
31	2.360659143245977E-4	0.0	0.0	27.974519045207803	0.0
32	2.360659143245977E-4	0.0	0.0	28.585044516129795	0.0
33	2.360659143245977E-4	0.0	0.0	29.188369976664884	0.0
34	2.360659143245977E-4	0.0	0.0	29.82185285775494	0.0
35	2.360659143245977E-4	0.0	0.0	30.45179475013013	0.0
36	2.360659143245977E-4	0.0	0.0	31.109061272088393	0.0
37	2.360659143245977E-4	0.0	0.0	31.738176933763445	0.0
38	2.9508239290574717E-4	0.0	0.0	32.38812541237765	0.0
39	2.9508239290574717E-4	0.0	0.0	33.020545996853244	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACACG	20	7.034234E-4	45.000004	27
AATTCCG	20	7.034234E-4	45.000004	13
GCGTAGT	25	3.891592E-5	45.0	14
TTGCGAA	30	2.1661144E-6	44.999996	13
GCGTAAG	220	0.0	42.954544	1
CTAACGG	150	0.0	42.0	2
CGTGCAA	105	0.0	40.714287	25
CGTATGG	190	0.0	40.263157	2
ATATACG	45	1.9292202E-8	40.0	1
CGTAAGG	400	0.0	39.9375	2
TACGGGA	530	0.0	39.905663	4
CACAACG	125	0.0	39.6	12
CGTCTAC	35	6.250044E-6	38.571426	41
ATATGCG	35	6.250044E-6	38.571426	1
GCGCATT	35	6.250044E-6	38.571426	26
TCATCGC	35	6.250044E-6	38.571426	12
AACTCGC	35	6.250044E-6	38.571426	19
TAATCCG	35	6.250044E-6	38.571426	1
TACGGGC	395	0.0	37.594936	4
GCTAACG	90	0.0	37.5	1
>>END_MODULE