##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935067.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1015911
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79791339989428	33.0	31.0	34.0	30.0	34.0
2	32.116288730016706	33.0	31.0	34.0	30.0	34.0
3	32.17772127676539	34.0	31.0	34.0	30.0	34.0
4	35.7546556735777	37.0	35.0	37.0	35.0	37.0
5	30.26638750835457	37.0	35.0	37.0	0.0	37.0
6	32.729423148287594	37.0	35.0	37.0	17.0	37.0
7	34.92569920002835	37.0	35.0	37.0	32.0	37.0
8	35.484405622146035	37.0	35.0	37.0	33.0	37.0
9	37.33031239941294	39.0	37.0	39.0	34.0	39.0
10	37.24074549837535	39.0	37.0	39.0	34.0	39.0
11	37.17051001514896	39.0	37.0	39.0	34.0	39.0
12	37.05299578407951	39.0	37.0	39.0	33.0	39.0
13	36.976110112007845	39.0	37.0	39.0	33.0	39.0
14	38.1104466828295	40.0	37.0	41.0	33.0	41.0
15	38.246760789084874	40.0	37.0	41.0	34.0	41.0
16	38.30446564708917	40.0	37.0	41.0	34.0	41.0
17	38.28674362222675	40.0	37.0	41.0	34.0	41.0
18	38.207510303560056	40.0	37.0	41.0	34.0	41.0
19	38.15537679973935	40.0	37.0	41.0	34.0	41.0
20	38.066671194622366	40.0	37.0	41.0	34.0	41.0
21	37.986726199440696	40.0	36.0	41.0	34.0	41.0
22	37.98542785736152	40.0	36.0	41.0	34.0	41.0
23	37.881641206759255	40.0	36.0	41.0	33.0	41.0
24	37.88860638382693	40.0	36.0	41.0	33.0	41.0
25	37.828940724138235	40.0	36.0	41.0	33.0	41.0
26	37.747864724370544	39.0	36.0	41.0	33.0	41.0
27	37.69020613026141	39.0	36.0	41.0	33.0	41.0
28	37.63873705472231	39.0	36.0	41.0	33.0	41.0
29	37.63454082099712	39.0	36.0	41.0	33.0	41.0
30	37.43089109183777	39.0	36.0	41.0	33.0	41.0
31	32.815951397317285	38.0	33.0	40.0	9.0	41.0
32	33.647066524528235	38.0	32.0	40.0	16.0	41.0
33	35.48967872185654	38.0	33.0	40.0	27.0	41.0
34	36.293069963805884	38.0	35.0	40.0	30.0	41.0
35	36.422035001097534	39.0	35.0	40.0	30.0	41.0
36	36.528539409456144	39.0	35.0	40.0	31.0	41.0
37	36.55562741224379	39.0	35.0	40.0	31.0	41.0
38	36.56101567952311	39.0	35.0	40.0	31.0	41.0
39	36.60526365006383	39.0	35.0	40.0	31.0	41.0
40	36.51119340178421	39.0	35.0	40.0	31.0	41.0
41	36.4627856180315	39.0	35.0	40.0	31.0	41.0
42	36.366532107635415	39.0	35.0	40.0	30.0	41.0
43	36.31487600783927	39.0	35.0	40.0	30.0	41.0
44	36.22254410081198	39.0	35.0	40.0	30.0	41.0
45	36.22982229742566	39.0	35.0	40.0	30.0	41.0
46	36.183971824303505	39.0	35.0	40.0	30.0	41.0
47	36.071360581783246	39.0	35.0	40.0	30.0	41.0
48	36.06053581465306	39.0	35.0	40.0	30.0	41.0
49	36.041057730450795	39.0	35.0	40.0	30.0	41.0
50	35.82191550244067	38.0	35.0	40.0	29.0	41.0
51	35.15487970895088	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	12.0
11	10.0
12	9.0
13	10.0
14	19.0
15	36.0
16	71.0
17	176.0
18	342.0
19	680.0
20	1162.0
21	1784.0
22	2894.0
23	4765.0
24	6302.0
25	7741.0
26	8322.0
27	9308.0
28	10890.0
29	13492.0
30	17163.0
31	23413.0
32	34427.0
33	41441.0
34	70352.0
35	101204.0
36	119541.0
37	119400.0
38	189019.0
39	231746.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.91707935045491	23.45894473039469	24.48915308526042	12.134822833889977
2	31.465650042178893	24.62321994741665	25.93406312167109	17.977066888733365
3	30.00686083721901	26.184183457015425	26.6069567117592	17.201998994006367
4	26.81691604874836	27.76237288502635	26.04598237444028	19.37472869178501
5	20.82072150021016	39.95281082693267	21.596183130215145	17.630284542642023
6	25.365115644972835	33.164814634352815	26.161543678530897	15.308526042143455
7	74.61155553980615	7.750482079630991	10.750744897929051	6.8872174826338135
8	72.38025771942621	6.699012019753699	12.035699977655522	8.88503028316457
9	66.96108222078509	8.439026647019276	15.101125984461236	9.498765147734398
10	35.42830031370858	24.64221767457976	22.20598064200506	17.7235013697066
11	26.0208817504683	25.742510908928047	26.99094704162077	21.245660298982884
12	23.294461818013588	23.58110110039167	32.23343383426304	20.891003247331703
13	21.231977998072665	26.87922465649058	33.451158615272405	18.437638730164355
14	17.622902006179675	31.04474703000558	29.637044977365147	21.6953059864496
15	15.589357729171157	28.21841677076043	35.55764235252891	20.634583147539498
16	17.88414536312728	29.04900133968428	31.19348053126701	21.873372765921424
17	16.980621333955433	28.04231866767857	27.341962041950524	27.63509795641547
18	18.073433598021875	27.674176182756167	31.559260604521462	22.6931296147005
19	18.40308845952057	29.428266846209954	29.503667151945397	22.66497754232408
20	20.521088953658342	29.775738229037778	29.181591694548047	20.521581122755833
21	19.31940888522715	30.0992901937276	31.057149691262325	19.524151229782923
22	17.32956922407573	28.432805629627005	27.233094237585775	27.004530908711494
23	17.127779894104897	29.402280317862488	28.55269802177553	24.917241766257085
24	20.665885102139853	26.00286836150017	28.47808518659607	24.853161349763905
25	17.8590447391553	30.09781368643513	25.781392267629744	26.261749306779826
26	18.830980272878232	29.511738725144234	28.30858214942057	23.348698852556964
27	21.09860017265292	27.351214820983333	28.97596344561679	22.574221560746956
28	17.95787229393126	26.72743970682471	29.161314327731464	26.153373671512565
29	22.07073257401485	24.636902248326873	28.750943734244437	24.541421443413842
30	22.144951673916317	26.533623516233213	29.390665127161732	21.930759682688738
31	22.793138375310434	30.23670380574676	23.097791046656646	23.872366772286156
32	24.387175648260527	29.63468256569719	23.621065231107842	22.357076554934437
33	21.77808882864739	27.035045392755862	28.461843606378906	22.725022172217844
34	20.435549964514607	28.005012250088836	26.79319349824936	24.766244287147202
35	24.177610046549354	27.308592977140716	25.531271932285403	22.982525044024527
36	24.233126720746206	25.43510209063589	29.94868644989571	20.38308473872219
37	21.911466654067137	26.962696535424858	29.483685086587307	21.642151723920698
38	21.731529632024852	28.17540119163982	26.123449790385184	23.969619385950146
39	25.71002774849372	24.48777501178745	28.66402667162773	21.138170568091102
40	23.55285059419575	26.088308916824403	29.747684590480862	20.611155898498986
41	21.416541409631357	27.66472653608436	25.75156682032186	25.167165233962425
42	25.7543229672678	23.49379030249697	27.57751417200916	23.174372558226064
43	25.932980349656614	20.842278506680213	30.125670457353053	23.09907068631012
44	22.02181096572436	22.098589344932776	28.037495410523167	27.8421042788197
45	22.58219470012629	21.57246057971614	28.637843275641274	27.207501444516303
46	24.20586055274527	23.19396088830616	30.49597848630441	22.104200072644158
47	19.824079077793233	23.977395657690487	32.688394947982644	23.510130316533633
48	20.26053463344722	22.265139367523336	31.456692564604577	26.017633434424862
49	23.204591740811942	19.246961594076648	33.66544904031948	23.882997624791933
50	21.282179246016632	19.312912253140286	33.250845792594035	26.15406270824905
51	19.59177526377803	19.8595152528125	28.942791248445975	31.605918234963497
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2276.0
1	2260.0
2	2244.0
3	2538.5
4	2833.0
5	2569.0
6	2305.0
7	2499.0
8	2693.0
9	3033.5
10	3374.0
11	3918.0
12	4462.0
13	4493.0
14	4524.0
15	4683.5
16	4843.0
17	4655.0
18	4467.0
19	4569.5
20	4672.0
21	4606.0
22	4540.0
23	4930.0
24	5320.0
25	5257.0
26	5377.0
27	5560.0
28	6868.5
29	8177.0
30	10057.5
31	11938.0
32	12704.5
33	13471.0
34	15710.5
35	17950.0
36	20445.0
37	22940.0
38	25122.5
39	27305.0
40	32322.5
41	37340.0
42	46837.5
43	56335.0
44	68614.0
45	80893.0
46	98949.5
47	117006.0
48	135672.0
49	154338.0
50	147368.0
51	140398.0
52	114185.0
53	87972.0
54	72861.0
55	57750.0
56	46921.5
57	36093.0
58	30950.0
59	25807.0
60	22611.0
61	19415.0
62	16993.0
63	14571.0
64	12121.0
65	9671.0
66	7895.0
67	6119.0
68	4963.5
69	3808.0
70	3146.5
71	2485.0
72	2207.5
73	1930.0
74	1747.5
75	1134.0
76	703.0
77	482.5
78	262.0
79	221.5
80	181.0
81	130.5
82	80.0
83	75.5
84	71.0
85	47.0
86	23.0
87	13.0
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1015911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.459608153063677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.98247857014424	19.43817963644371
2	13.642886105601198	8.038281574957763
3	6.320009836317932	5.585550399043031
4	3.530960930014851	4.160829016080592
5	2.0599253963696595	3.034229750079728
6	1.3943205267525494	2.4645681814742066
7	0.9251205951091597	1.9077583158371447
8	0.6885324069894369	1.6227115928475657
9	0.5139683706982056	1.3627176123453937
>10	4.193805094362738	28.7974003860114
>50	0.6504116644237479	12.300329211391501
>100	0.08331873736155071	4.885966677107836
>500	0.008256811810603925	1.5920217732948982
>1k	0.004879025160811411	2.334353475906326
>5k	0.0011259288832641717	2.4751023971789428
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	9021	0.8879714856911679	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	7186	0.7073454269123969	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	6179	0.6082225706779433	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	2464	0.24254093124299272	No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	2160	0.2126170501156105	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	2035	0.20031282267836456	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	1897	0.18672895558764496	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1794	0.17659027217935427	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	1487	0.14637108959347817	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	1485	0.14617422195448224	No Hit
GGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	1446	0.1423353029940615	No Hit
AAAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	1441	0.14184313389657166	No Hit
TGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCTT	1312	0.12914517118133378	Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	1260	0.12402661256743948	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	1186	0.11674250992458986	No Hit
GGCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	1146	0.11280515714467113	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9686763899593566E-4	0.0	0.0	0.9350228514111965	0.0
2	1.9686763899593566E-4	0.0	0.0	2.8115651863204554	0.0
3	1.9686763899593566E-4	0.0	0.0	3.9859790867507097	0.0
4	1.9686763899593566E-4	0.0	0.0	5.322415054074619	0.0
5	1.9686763899593566E-4	0.0	0.0	8.479975115930431	0.0
6	1.9686763899593566E-4	0.0	0.0	10.503479143350155	0.0
7	1.9686763899593566E-4	0.0	0.0	12.167207560504808	0.0
8	1.9686763899593566E-4	0.0	0.0	15.131935769964102	0.0
9	1.9686763899593566E-4	0.0	0.0	16.08182212811949	0.0
10	1.9686763899593566E-4	0.0	0.0	18.18308887294261	0.0
11	1.9686763899593566E-4	0.0	0.0	21.430617445819564	0.0
12	2.953014584939035E-4	0.0	0.0	23.920008740923173	0.0
13	2.953014584939035E-4	0.0	0.0	24.883872701447274	0.0
14	2.953014584939035E-4	0.0	0.0	25.269241104781816	0.0
15	2.953014584939035E-4	0.0	0.0	25.967727487939396	0.0
16	2.953014584939035E-4	0.0	0.0	27.457228044582646	0.0
17	2.953014584939035E-4	0.0	0.0	29.18464314295248	0.0
18	8.859043754817105E-4	0.0	0.0	31.291028446389497	0.0
19	8.859043754817105E-4	0.0	0.0	32.52755408692297	0.0
20	8.859043754817105E-4	0.0	0.0	33.577350771868794	0.0
21	9.843381949796784E-4	0.0	0.0	34.70904439463693	0.0
22	9.843381949796784E-4	0.0	0.0	35.761498792709205	0.0
23	0.0010827720144776462	0.0	0.0	36.80696438959712	0.0
24	0.0010827720144776462	0.0	0.0	37.59571458523433	0.0
25	0.0010827720144776462	0.0	0.0	38.365565487527945	0.0
26	0.001181205833975614	0.0	0.0	39.02467834288633	0.0
27	0.001181205833975614	0.0	0.0	39.64510670718202	0.0
28	0.001181205833975614	0.0	0.0	40.27764243127597	0.0
29	0.001181205833975614	0.0	0.0	40.941480109970264	0.0
30	0.001181205833975614	0.0	0.0	41.62844973624658	0.0
31	0.001181205833975614	0.0	0.0	42.27230534958279	0.0
32	0.001181205833975614	0.0	0.0	42.94323026328094	0.0
33	0.001181205833975614	0.0	0.0	43.61159589767214	0.0
34	0.0013780734729715496	0.0	0.0	44.2914782889446	0.0
35	0.0013780734729715496	0.0	0.0	44.977266709386946	0.0
36	0.0013780734729715496	0.0	0.0	45.63775763821831	0.0
37	0.0013780734729715496	0.0	0.0	46.29263783933829	0.0
38	0.0013780734729715496	0.0	0.0	46.948797680111745	0.0
39	0.0013780734729715496	0.0	0.0	47.64255923993342	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCA	20	7.032899E-4	45.000004	13
CTATACG	20	7.032899E-4	45.000004	1
TTGTCGT	20	7.032899E-4	45.000004	42
CCGTGCA	20	7.032899E-4	45.000004	45
GCTTGCG	20	7.032899E-4	45.000004	1
CGTTAGT	70	0.0	45.000004	28
CGAGGTT	20	7.032899E-4	45.000004	37
CACTACG	20	7.032899E-4	45.000004	1
CTCGCAC	20	7.032899E-4	45.000004	38
ACGTCAT	20	7.032899E-4	45.000004	10
CAATCGT	20	7.032899E-4	45.000004	15
TGCACGT	20	7.032899E-4	45.000004	28
AATCGTC	20	7.032899E-4	45.000004	12
AGTCGAC	20	7.032899E-4	45.000004	12
ACGAACG	20	7.032899E-4	45.000004	1
CGCCTAA	25	3.8904847E-5	45.0	14
AATTGCG	45	3.8380676E-10	45.0	1
TCCACCG	25	3.8904847E-5	45.0	31
TATTACG	25	3.8904847E-5	45.0	1
CACGTTA	45	3.8380676E-10	45.0	16
>>END_MODULE