FastQCFastQC Report
Sat 14 Jan 2017
SRR2935066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900311
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC155630.81897121050186Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT154310.8120249790692156Illumina PCR Primer Index 10 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC130140.6848352717002638Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC76840.4043548661245449No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG71770.3776750226673423No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC59930.31536943163513764No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC50700.26679843457202534Illumina PCR Primer Index 10 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC48210.2536953161877188No Hit
TGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCTT38900.2047033354014159Illumina PCR Primer Index 10 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC38450.2023353019584689Illumina PCR Primer Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG32000.168393489276229Illumina PCR Primer Index 10 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG27690.14571299118933692Illumina PCR Primer Index 10 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG27460.144502662985164Illumina PCR Primer Index 10 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG27450.1444500400197652Illumina PCR Primer Index 10 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC26180.13776692341411484No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCATTAGCT25430.13382020100920325No Hit
ACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG24870.13087331494686924Illumina PCR Primer Index 10 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC22000.11577052387740742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21060.11082396512991821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG750.045.0000042
GATTACG453.8562575E-1045.0000041
TCGTTAC253.8917715E-545.020
GATCGAC207.0344517E-445.09
ATGCGAA800.045.026
CGGTAAT253.8917715E-545.030
TTCGACA253.8917715E-545.032
CGTAAGG2500.043.1999972
ACGGGTC1350.041.6666645
ACGTAAG650.041.5384641
TATTGCG603.6379788E-1241.2499961
TACGGGT1400.040.1785744
GAATGCG1350.040.01
CTACGAA1300.039.80769311
CGTTATT3450.039.7826081
TATACGC2500.039.626
CCCGTTA4950.039.54545228
TATGGGA10400.039.3750044
GTACGAG403.4598088E-739.3751
CTACGGG3800.039.0789453
Produced by FastQC (version 0.11.5)