##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935066.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1900311
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70746367305141	33.0	31.0	34.0	30.0	34.0
2	32.0274712928568	33.0	31.0	34.0	30.0	34.0
3	32.098061843561396	33.0	31.0	34.0	30.0	34.0
4	35.69908925433784	37.0	35.0	37.0	33.0	37.0
5	30.261608757724392	37.0	35.0	37.0	0.0	37.0
6	32.70393267207315	37.0	35.0	37.0	17.0	37.0
7	34.87482838335409	37.0	35.0	37.0	32.0	37.0
8	35.38198221238524	37.0	35.0	37.0	33.0	37.0
9	37.17169031805847	39.0	37.0	39.0	34.0	39.0
10	37.16986061755155	39.0	37.0	39.0	34.0	39.0
11	37.132894036818186	39.0	37.0	39.0	34.0	39.0
12	37.0552262234971	39.0	37.0	39.0	33.0	39.0
13	37.00729617415254	39.0	37.0	39.0	33.0	39.0
14	38.101952785622984	40.0	37.0	41.0	33.0	41.0
15	38.19015150677968	40.0	37.0	41.0	33.0	41.0
16	38.266993139544	40.0	37.0	41.0	34.0	41.0
17	38.230119701459394	40.0	37.0	41.0	34.0	41.0
18	38.1743204138691	40.0	37.0	41.0	34.0	41.0
19	38.153888495093696	40.0	37.0	41.0	34.0	41.0
20	38.05492206275709	40.0	37.0	41.0	34.0	41.0
21	38.008001848118546	40.0	37.0	41.0	33.0	41.0
22	37.98666270941967	40.0	37.0	41.0	33.0	41.0
23	37.894232575615256	40.0	36.0	41.0	33.0	41.0
24	37.872250384279205	40.0	36.0	41.0	33.0	41.0
25	37.805699698628274	40.0	36.0	41.0	33.0	41.0
26	37.73412983453761	39.0	36.0	41.0	33.0	41.0
27	37.66445281851234	39.0	36.0	41.0	33.0	41.0
28	37.58846420401713	39.0	36.0	41.0	33.0	41.0
29	37.62043949648242	39.0	36.0	41.0	33.0	41.0
30	37.4580913334712	39.0	36.0	41.0	33.0	41.0
31	32.9110682409353	38.0	33.0	40.0	9.0	41.0
32	33.79573185652243	38.0	33.0	40.0	16.0	41.0
33	35.69157837848647	38.0	34.0	40.0	28.0	41.0
34	36.46009258484532	38.0	35.0	40.0	30.0	41.0
35	36.67184213531364	39.0	35.0	40.0	31.0	41.0
36	36.80486930823428	39.0	35.0	40.0	31.0	41.0
37	36.85684395869939	39.0	35.0	41.0	31.0	41.0
38	36.82184916047952	39.0	35.0	41.0	31.0	41.0
39	36.83882322419856	39.0	35.0	41.0	31.0	41.0
40	36.76599935484244	39.0	35.0	41.0	31.0	41.0
41	36.636817868233145	39.0	35.0	41.0	31.0	41.0
42	36.58420647988671	39.0	35.0	41.0	31.0	41.0
43	36.56858219523015	39.0	35.0	41.0	31.0	41.0
44	36.49741594928409	39.0	35.0	41.0	31.0	41.0
45	36.47820014723906	39.0	35.0	40.0	31.0	41.0
46	36.374065087240986	39.0	35.0	40.0	30.0	41.0
47	36.233078690803765	39.0	35.0	40.0	30.0	41.0
48	36.256609576011506	39.0	35.0	40.0	30.0	41.0
49	36.26438251423057	39.0	35.0	40.0	30.0	41.0
50	36.05746480444517	39.0	35.0	40.0	29.0	41.0
51	35.36530494219104	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	20.0
11	15.0
12	23.0
13	27.0
14	35.0
15	58.0
16	121.0
17	247.0
18	572.0
19	976.0
20	1784.0
21	3057.0
22	4938.0
23	7684.0
24	10384.0
25	12496.0
26	14250.0
27	16749.0
28	19874.0
29	25064.0
30	32696.0
31	45020.0
32	63504.0
33	78006.0
34	129110.0
35	180416.0
36	230597.0
37	227409.0
38	357531.0
39	437348.0
40	284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06521985085599	21.471801194646563	23.66917836080515	12.793800593692294
2	30.468381228125292	27.235173611056297	23.81836446771081	18.4780806931076
3	29.462335375630623	27.185550154685206	26.17271593965409	17.179398530030085
4	27.396410377038283	25.746996149577623	28.10655729509538	18.750036178288713
5	22.262356003833055	37.972731831789645	21.154958319980256	18.609953844397047
6	24.079321753123565	33.087899822713226	25.508508870390163	17.32426955377304
7	71.02242738162332	7.551290288800096	13.9993401080139	7.426942221562681
8	68.18078725008696	8.239388184355088	13.739698396736113	9.84012616882184
9	61.527665734713956	9.145450402591997	16.931544363001635	12.395339499692419
10	33.651860142892396	26.27585695183578	23.07853819716878	16.993744708103044
11	25.65590579647226	25.320907998743365	27.37436135453618	21.648824850248197
12	23.01333834303964	21.735547497225454	33.16593968039968	22.085174479335222
13	21.60804205206411	24.340647399294117	34.935544760831256	19.11576578781052
14	17.034317014425536	29.589630328930372	29.748393815538616	23.627658841105482
15	15.258818161869295	27.089671111728553	36.591378990070574	21.06013173633158
16	16.33511567317139	28.40087754057099	30.645720621519317	24.618286164738297
17	15.969280817718785	28.529277576144114	28.452500669627234	27.04894093650987
18	16.69074167333663	27.247540007925018	31.949822950032914	24.11189536870544
19	18.262747518695623	28.481653792458182	28.853908649689448	24.401690039156748
20	20.021617514185834	29.112603147590054	29.69824412951354	21.167535208710575
21	19.51685803008034	29.89147565845801	30.144223761268552	20.447442550193102
22	16.891656155229327	27.214334916758364	27.762981954006477	28.131026974005835
23	17.65842538405556	28.196489942961968	28.96173310579163	25.183351567190844
24	20.96683121867947	24.834619175492854	28.341834573393516	25.856715032434163
25	17.727308845762614	29.360720429445497	25.000434139464538	27.91153658532735
26	16.56428868748326	27.952056268684444	26.854920063084407	28.62873498074789
27	20.51527355259218	27.422406121945304	25.397474413398648	26.664845912063868
28	15.488359536938953	28.22453798351954	28.04630399971373	28.240798479827777
29	17.216287228774657	24.570978118844756	27.33642019648363	30.87631445589696
30	18.82602374032461	26.518554068255145	26.852499406676067	27.802922784744183
31	20.81617166874264	31.59719645889541	22.305717327321688	25.28091454504026
32	19.38682668257985	28.374145074148387	25.849663555070723	26.389364688201038
33	17.80734837613422	25.743312541999703	27.624057325353586	28.82528175651249
34	17.762303117752833	27.932796263348475	27.66768176366921	26.637218855229488
35	20.144913122115273	23.39822271196662	26.399257805696013	30.05760636022209
36	18.503444962429832	25.731261882923373	26.1946597162254	29.570633438421396
37	17.84050084433548	28.248902416499195	29.204851205934183	24.705745533231138
38	19.865274683985938	26.648374923894036	28.19848961564712	25.287860776472904
39	21.062973376463116	23.29697612653929	30.476958771485297	25.1630917255123
40	22.284352403369763	22.58456642097004	31.51915660120896	23.611924574451233
41	19.607895760220302	25.88671012271149	24.566663035682055	29.93873108138615
42	21.50269087533567	22.504421644667634	27.47744974375247	28.515437736244227
43	21.637405666756653	21.369870510669042	28.76123960762212	28.231484214952186
44	18.930796064433665	22.72827973947422	29.09934216030955	29.241582035782564
45	19.302314200149347	20.50411748392763	29.65214641182417	30.541421904098854
46	22.717860392325257	22.28540486267774	29.556898844452302	25.4398359005447
47	17.556757814905033	22.29208797928339	33.86045757773333	26.290696628078248
48	17.85691920953991	21.173271111939044	31.125431574094975	29.844378104426067
49	20.377138268420275	18.620004830788222	34.54566121019138	26.457195690600116
50	19.391983733188937	18.32684229055139	33.41616188087107	28.865012095388597
51	17.902332828679096	18.379254764088614	29.090238387295553	34.628174019936736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2765.0
1	3364.0
2	3963.0
3	4900.5
4	5838.0
5	5033.0
6	4228.0
7	4366.5
8	4505.0
9	5026.5
10	5548.0
11	6139.5
12	6731.0
13	7249.0
14	7767.0
15	7737.0
16	7707.0
17	7671.0
18	7635.0
19	7484.5
20	7334.0
21	7074.5
22	6815.0
23	7530.0
24	8245.0
25	10028.0
26	13711.5
27	15612.0
28	17287.5
29	18963.0
30	20324.0
31	21685.0
32	23479.0
33	25273.0
34	29239.0
35	33205.0
36	36440.0
37	39675.0
38	45135.5
39	50596.0
40	57752.5
41	64909.0
42	79231.0
43	93553.0
44	114674.5
45	135796.0
46	183392.0
47	230988.0
48	256512.5
49	282037.0
50	270538.5
51	259040.0
52	207645.0
53	156250.0
54	127840.0
55	99430.0
56	84249.5
57	69069.0
58	62522.5
59	55976.0
60	51771.0
61	47566.0
62	41775.5
63	35985.0
64	30675.0
65	25365.0
66	20783.5
67	16202.0
68	13772.5
69	11343.0
70	9918.5
71	8494.0
72	6916.0
73	5338.0
74	4217.5
75	2532.5
76	1968.0
77	1536.5
78	1105.0
79	796.0
80	487.0
81	364.5
82	242.0
83	172.0
84	102.0
85	64.5
86	27.0
87	32.0
88	37.0
89	20.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1900311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.39658810228369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.96659055887906	20.71125872289122
2	14.061090730887798	8.829405478930466
3	6.510336111540267	6.13207023904359
4	3.4568080660252924	4.341279159905919
5	2.0384661229571552	3.2000440611472487
6	1.315622029598693	2.4783625776960383
7	0.8903239215173473	1.9567193409043073
8	0.6516149294842727	1.6366788433853072
9	0.49011013187174274	1.3849007341619743
>10	4.253914575221033	29.139026888553826
>50	0.2700675636507133	5.434311591499533
>100	0.07710654775217285	4.508870731372229
>500	0.009706955740557254	2.096612694204668
>1k	0.0069597041158712385	4.129167174390411
>5k	7.326004332496042E-4	1.4907047937402103
>10k+	5.494503249372032E-4	2.5305869681730897
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	15563	0.81897121050186	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	15431	0.8120249790692156	Illumina PCR Primer Index 10 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	13014	0.6848352717002638	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	7684	0.4043548661245449	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	7177	0.3776750226673423	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	5993	0.31536943163513764	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	5070	0.26679843457202534	Illumina PCR Primer Index 10 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	4821	0.2536953161877188	No Hit
TGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCTT	3890	0.2047033354014159	Illumina PCR Primer Index 10 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	3845	0.2023353019584689	Illumina PCR Primer Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	3200	0.168393489276229	Illumina PCR Primer Index 10 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	2769	0.14571299118933692	Illumina PCR Primer Index 10 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	2746	0.144502662985164	Illumina PCR Primer Index 10 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	2745	0.1444500400197652	Illumina PCR Primer Index 10 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	2618	0.13776692341411484	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCATTAGCT	2543	0.13382020100920325	No Hit
ACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	2487	0.13087331494686924	Illumina PCR Primer Index 10 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	2200	0.11577052387740742	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2106	0.11082396512991821	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0524593079764312E-4	0.0	0.0	0.862016796198096	0.0
2	1.0524593079764312E-4	0.0	0.0	2.970303281936483	0.0
3	1.0524593079764312E-4	0.0	0.0	4.179210666043611	0.0
4	1.0524593079764312E-4	0.0	0.0	5.576245151451525	0.0
5	1.0524593079764312E-4	0.0	0.0	9.20744025583181	0.0
6	1.0524593079764312E-4	0.0	0.0	11.562633695221466	0.0
7	1.0524593079764312E-4	0.0	0.0	13.54157293200955	0.0
8	1.578688961964647E-4	0.0	0.0	16.695477740222522	0.0
9	1.578688961964647E-4	0.0	0.0	17.809032311026986	0.0
10	1.578688961964647E-4	0.0	0.0	20.32946186176894	0.0
11	1.578688961964647E-4	0.0	0.0	23.516413892252373	0.0
12	1.578688961964647E-4	0.0	0.0	26.650795580302383	0.0
13	1.578688961964647E-4	0.0	0.0	27.806922130114494	0.0
14	1.578688961964647E-4	0.0	0.0	28.327784241632028	0.0
15	2.1049186159528623E-4	0.0	0.0	29.237845805239246	0.0
16	2.1049186159528623E-4	0.0	0.0	30.797695745591117	0.0
17	2.1049186159528623E-4	0.0	0.0	32.59945345788137	0.0
18	2.1049186159528623E-4	0.0	0.0	34.60338860323389	0.0
19	2.1049186159528623E-4	0.0	0.0	35.938433235401995	0.0
20	2.1049186159528623E-4	0.0	0.0	36.98447254159977	0.0
21	2.1049186159528623E-4	0.0	0.0	38.094869734480305	0.0
22	2.1049186159528623E-4	0.0	0.0	39.18479659382069	0.0
23	2.1049186159528623E-4	0.0	0.0	40.18694834687585	0.0
24	2.1049186159528623E-4	0.0	0.0	40.996921030294516	0.0
25	2.1049186159528623E-4	0.0	0.0	41.72795926561494	0.0
26	2.1049186159528623E-4	0.0	0.0	42.37559010077824	0.0
27	2.1049186159528623E-4	0.0	0.0	42.99501502648777	0.0
28	2.1049186159528623E-4	0.0	0.0	43.6456979936442	0.0
29	2.1049186159528623E-4	0.0	0.0	44.336163922642136	0.0
30	2.1049186159528623E-4	0.0	0.0	45.054730515163044	0.0
31	2.631148269941078E-4	0.0	0.0	45.7088865980358	0.0
32	2.631148269941078E-4	0.0	0.0	46.40850892301313	0.0
33	3.157377923929294E-4	0.0	0.0	47.08492452024958	0.0
34	3.6836075779175093E-4	0.0	0.0	47.73066092865852	0.0
35	3.6836075779175093E-4	0.0	0.0	48.40002504853153	0.0
36	3.6836075779175093E-4	0.0	0.0	49.08822819001732	0.0
37	3.6836075779175093E-4	0.0	0.0	49.75680296540935	0.0
38	4.2098372319057247E-4	0.0	0.0	50.40306560347227	0.0
39	4.2098372319057247E-4	0.0	0.0	51.039750861832616	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGG	75	0.0	45.000004	2
GATTACG	45	3.8562575E-10	45.000004	1
TCGTTAC	25	3.8917715E-5	45.0	20
GATCGAC	20	7.0344517E-4	45.0	9
ATGCGAA	80	0.0	45.0	26
CGGTAAT	25	3.8917715E-5	45.0	30
TTCGACA	25	3.8917715E-5	45.0	32
CGTAAGG	250	0.0	43.199997	2
ACGGGTC	135	0.0	41.666664	5
ACGTAAG	65	0.0	41.538464	1
TATTGCG	60	3.6379788E-12	41.249996	1
TACGGGT	140	0.0	40.178574	4
GAATGCG	135	0.0	40.0	1
CTACGAA	130	0.0	39.807693	11
CGTTATT	345	0.0	39.782608	1
TATACGC	250	0.0	39.6	26
CCCGTTA	495	0.0	39.545452	28
TATGGGA	1040	0.0	39.375004	4
GTACGAG	40	3.4598088E-7	39.375	1
CTACGGG	380	0.0	39.078945	3
>>END_MODULE