##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935065.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1575703
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27405037624476	33.0	31.0	34.0	30.0	34.0
2	32.63067215077968	34.0	31.0	34.0	31.0	34.0
3	32.66743225087469	34.0	31.0	34.0	31.0	34.0
4	36.14763505559106	37.0	35.0	37.0	35.0	37.0
5	30.45988742802419	37.0	35.0	37.0	0.0	37.0
6	32.996037958930074	37.0	35.0	37.0	17.0	37.0
7	35.25356745528821	37.0	35.0	37.0	32.0	37.0
8	35.81376439595533	37.0	35.0	37.0	35.0	37.0
9	37.71869318012341	39.0	38.0	39.0	35.0	39.0
10	37.687287515477216	39.0	37.0	39.0	35.0	39.0
11	37.608692754916376	39.0	37.0	39.0	35.0	39.0
12	37.584908450386905	39.0	37.0	39.0	35.0	39.0
13	37.596941174828	39.0	37.0	39.0	35.0	39.0
14	38.87445095934957	40.0	38.0	41.0	35.0	41.0
15	38.95231715621535	40.0	38.0	41.0	35.0	41.0
16	38.99440249844038	40.0	38.0	41.0	35.0	41.0
17	38.975804450457986	40.0	38.0	41.0	35.0	41.0
18	38.92089118317348	40.0	38.0	41.0	35.0	41.0
19	38.90050472709641	40.0	38.0	41.0	35.0	41.0
20	38.82334361234319	40.0	38.0	41.0	35.0	41.0
21	38.77805208215	40.0	38.0	41.0	35.0	41.0
22	38.763160951016786	40.0	38.0	41.0	35.0	41.0
23	38.68865325508678	40.0	38.0	41.0	35.0	41.0
24	38.69151927742728	40.0	38.0	41.0	35.0	41.0
25	38.64155427767796	40.0	38.0	41.0	35.0	41.0
26	38.59057766596878	40.0	38.0	41.0	35.0	41.0
27	38.553300336421266	40.0	38.0	41.0	35.0	41.0
28	38.52926598476997	40.0	38.0	41.0	35.0	41.0
29	38.550980102214695	40.0	38.0	41.0	35.0	41.0
30	38.38835554669884	40.0	38.0	41.0	34.0	41.0
31	33.62701854346917	39.0	34.0	41.0	9.0	41.0
32	34.56138053935291	39.0	34.0	41.0	16.0	41.0
33	36.60139950231738	39.0	34.0	41.0	30.0	41.0
34	37.54248040398476	39.0	36.0	41.0	33.0	41.0
35	37.665054264667894	39.0	37.0	41.0	33.0	41.0
36	37.778125700084345	40.0	37.0	41.0	34.0	41.0
37	37.904207201484034	40.0	37.0	41.0	34.0	41.0
38	37.8993033585644	40.0	37.0	41.0	34.0	41.0
39	37.90655282118521	40.0	37.0	41.0	34.0	41.0
40	37.898462464055726	40.0	37.0	41.0	34.0	41.0
41	37.83527860263006	40.0	37.0	41.0	34.0	41.0
42	37.715855081826966	40.0	37.0	41.0	34.0	41.0
43	37.70094364229807	40.0	37.0	41.0	33.0	41.0
44	37.670521030930324	40.0	37.0	41.0	33.0	41.0
45	37.67098939330572	40.0	37.0	41.0	34.0	41.0
46	37.60912748151143	40.0	37.0	41.0	33.0	41.0
47	37.48973950040077	40.0	36.0	41.0	33.0	41.0
48	37.489457721410695	40.0	36.0	41.0	33.0	41.0
49	37.46621349327887	40.0	36.0	41.0	33.0	41.0
50	37.30768932977852	40.0	36.0	41.0	33.0	41.0
51	36.672962480873615	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	6.0
11	7.0
12	13.0
13	14.0
14	15.0
15	29.0
16	63.0
17	99.0
18	210.0
19	374.0
20	665.0
21	1212.0
22	2287.0
23	3957.0
24	5618.0
25	6785.0
26	7552.0
27	8132.0
28	9015.0
29	10803.0
30	13904.0
31	19727.0
32	31665.0
33	40090.0
34	79385.0
35	126331.0
36	186843.0
37	182775.0
38	315910.0
39	521825.0
40	391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.98367712697126	21.523091597845536	26.068047087553936	12.425184187629268
2	31.214765726789885	25.17930092155692	25.57880514284735	18.02712820880585
3	30.726856520549877	25.57429921755559	27.112533262930892	16.58631099896364
4	28.125033715110014	26.73587598678177	26.515529893641126	18.623560404467085
5	21.778406209799687	39.14652697875171	21.612512002579166	17.462554808869438
6	26.306543809334627	32.943644836622134	24.940740736039725	15.80907061800352
7	78.18002504279042	6.190125931092344	9.919064696836905	5.710784329280328
8	76.80977950794026	5.532006983549565	10.441498175734894	7.2167153327752755
9	70.656081761601	8.255489771866907	13.001752233764865	8.086676232767216
10	33.95798573715986	31.901824138178327	19.75860933183474	14.381580792827076
11	22.877598126042788	24.552913842265962	32.293966566034335	20.275521465656915
12	21.583382147523995	21.781642860361377	35.52890360683454	21.106071385280096
13	21.87404606071068	24.108731150476963	36.20161921377316	17.815603575039205
14	18.32432888685241	28.471609180156417	32.027482336455535	21.176579596535642
15	16.841117901025765	27.558810258024515	35.680264618395725	19.919807222553995
16	18.96785117499935	28.6316647236186	31.174910500265597	21.225573601116455
17	18.532616870057364	27.545355945885742	29.44533328933181	24.476693894725084
18	19.115975535998853	26.247966780541766	32.652727068489426	21.983330614969955
19	19.08671875347067	28.155369381158756	30.847183764960782	21.910728100409784
20	20.57399141843355	29.32545029107643	30.063533546613797	20.037024743876223
21	19.83311575849002	29.318786598743547	31.71010019020082	19.137997452565617
22	18.507548694138425	26.106950358030666	29.968464869331342	25.417036078499567
23	17.502219644184215	27.712011717944307	30.629439685016784	24.156328952854693
24	20.05174833074507	25.62760875621865	30.938254226843508	23.382388686192765
25	17.773273262791275	29.037134536140375	28.547448345278266	24.64214385579008
26	17.17569871987297	28.709725119518083	31.650126959204876	22.464449201404072
27	19.401879668947767	28.493821487932685	30.257542189105436	21.84675665401411
28	16.319699841911834	27.423505571798746	32.14914231933302	24.1076522669564
29	17.86999199722283	25.349510662859686	31.366063274614568	25.414434065302917
30	20.99158280462752	28.004262224543584	28.926136460995505	22.078018509833388
31	22.120158430871808	28.46875331201375	26.43245586255786	22.978632394556588
32	19.910224198341947	27.748312975224394	27.593842240574524	24.74762058585914
33	20.916251349397697	28.139820765715367	26.528159177205353	24.415768707681586
34	19.57475488718369	24.125675968123435	30.341822031182275	25.957747113510603
35	20.709359568395822	25.192882161168694	28.40484532935458	25.692912941080902
36	20.966705019918095	29.00876624592325	28.975765102941352	21.048763631217305
37	18.980924704719097	27.532028561219974	30.622458674001386	22.864588060059543
38	20.879125063543068	26.9418158117361	27.45891833676778	24.72014078795306
39	22.61841222616191	25.07357033654185	27.251264990927858	25.056752446368385
40	23.19555144592604	22.791604763080354	29.816659611614625	24.19618417937898
41	20.681879770489743	26.495729207851987	27.025968726339926	25.796422295318344
42	21.2455012143786	26.400914385515545	29.884756200883032	22.468828199222823
43	21.35021637960961	25.00744112310505	30.433400202957028	23.20894229432831
44	19.985428726098764	24.21357324318098	29.186210853187433	26.614787177532822
45	20.967085802337117	21.738931765694424	29.017587705297252	28.276394726671207
46	24.010171967686805	22.55621776438834	29.340998906519822	24.092611361405037
47	18.99939265204166	23.9107877563221	32.48937141072905	24.60044818090719
48	19.20634789678004	23.488626981099863	31.326017656880772	25.979007465239324
49	21.125237433704193	21.573672195838935	32.86044387806585	24.440646492391018
50	20.095411381459577	20.390835074884038	32.516978136108136	26.996775407548252
51	19.35948589296333	19.947667802879096	29.12128745074421	31.571558853413368
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1930.0
1	2316.0
2	2702.0
3	3427.0
4	4152.0
5	3500.5
6	2849.0
7	3034.5
8	3220.0
9	3595.5
10	3971.0
11	4386.0
12	4801.0
13	4953.0
14	5105.0
15	5186.0
16	5267.0
17	5417.5
18	5568.0
19	5677.5
20	5787.0
21	6203.0
22	6619.0
23	7978.0
24	9337.0
25	10823.5
26	14314.5
27	16319.0
28	18708.0
29	21097.0
30	24158.0
31	27219.0
32	30843.0
33	34467.0
34	38148.5
35	41830.0
36	45016.5
37	48203.0
38	52803.5
39	57404.0
40	64212.0
41	71020.0
42	81399.0
43	91778.0
44	106936.0
45	122094.0
46	148983.0
47	175872.0
48	195453.5
49	215035.0
50	205273.5
51	195512.0
52	159664.5
53	123817.0
54	100814.0
55	77811.0
56	65443.0
57	53075.0
58	45880.5
59	38686.0
60	34313.5
61	29941.0
62	26205.5
63	22470.0
64	18583.0
65	14696.0
66	11625.0
67	8554.0
68	6848.5
69	5143.0
70	4516.5
71	3890.0
72	3148.5
73	2407.0
74	2048.5
75	1319.0
76	948.0
77	692.0
78	436.0
79	432.0
80	428.0
81	301.5
82	175.0
83	116.0
84	57.0
85	29.5
86	2.0
87	4.0
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1575703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.80572788650034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.98251888173213	19.36851275168278
2	14.466158966530923	8.623487954385553
3	6.34879942971102	5.676917646238055
4	3.4328590355814876	4.092754491490233
5	1.9810125809315355	2.952276096348953
6	1.2637032690986454	2.259935746082108
7	0.879131953431979	1.8342217446224736
8	0.6475210405418607	1.54398687481394
9	0.4839829599409819	1.2982917965133511
>10	4.98608545200757	34.524905973493674
>50	0.44151460412951593	8.320283131220464
>100	0.074392114245705	4.158446792453657
>500	0.007344443761961077	1.459258923315613
>1k	0.004264515732751593	2.1463428489803076
>5k	7.107526221252655E-4	1.7403772283588965
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	9408	0.5970668330262746	No Hit
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT	7983	0.5066310085085832	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	7255	0.460429408333931	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC	3386	0.21488821180133563	No Hit
TGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCTT	2958	0.1877257325777764	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	2642	0.16767119184262516	No Hit
CGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTG	2096	0.133019991711636	No Hit
ACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	2080	0.13200457192757772	No Hit
GCCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTG	1822	0.11563092790963779	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTC	1784	0.11321930592249935	No Hit
GGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTG	1700	0.10788835205619333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5245912459391142	0.0
2	0.0	0.0	0.0	1.93380351500251	0.0
3	0.0	0.0	0.0	2.7649880719907243	0.0
4	0.0	0.0	0.0	3.7459470471275362	0.0
5	0.0	0.0	0.0	6.251939610446892	0.0
6	0.0	0.0	0.0	7.965079713626236	0.0
7	0.0	0.0	0.0	9.43229783785396	0.0
8	0.0	0.0	0.0	11.954346726508739	0.0
9	0.0	0.0	0.0	12.917408927951524	0.0
10	0.0	0.0	0.0	14.855401049563275	0.0
11	0.0	0.0	0.0	17.783744779314375	0.0
12	0.0	0.0	0.0	20.153734555306425	0.0
13	0.0	0.0	0.0	21.08931695884313	0.0
14	0.0	0.0	0.0	21.474859158102763	0.0
15	0.0	0.0	0.0	22.1374205672008	0.0
16	0.0	0.0	0.0	23.477330436002216	0.0
17	0.0	0.0	0.0	25.14725173462258	0.0
18	0.0	0.0	0.0	27.0830226254567	0.0
19	0.0	0.0	0.0	28.244980177101905	0.0
20	0.0	0.0	0.0	29.184814650984354	0.0
21	0.0	0.0	0.0	30.195918900960397	0.0
22	0.0	0.0	0.0	31.269534931392528	0.0
23	0.0	0.0	0.0	32.31433842545201	0.0
24	0.0	0.0	0.0	33.10268496030026	0.0
25	0.0	0.0	0.0	33.81214607067449	0.0
26	0.0	0.0	0.0	34.45649338739597	0.0
27	0.0	0.0	0.0	35.08294393042344	0.0
28	0.0	0.0	0.0	35.73750890872201	0.0
29	0.0	0.0	0.0	36.432944533328936	0.0
30	0.0	0.0	0.0	37.17946846582129	0.0
31	0.0	0.0	0.0	37.85427837606453	0.0
32	0.0	0.0	0.0	38.58480944695796	0.0
33	0.0	0.0	0.0	39.316292473898955	0.0
34	0.0	0.0	0.0	40.01122038861384	0.0
35	0.0	0.0	0.0	40.73743592542503	0.0
36	0.0	0.0	0.0	41.44391424018359	0.0
37	0.0	0.0	0.0	42.15451769781488	0.0
38	0.0	0.0	0.0	42.866961603804775	0.0
39	0.0	0.0	0.0	43.55776437564693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAC	20	7.034085E-4	45.0	3
GGCCGTA	20	7.034085E-4	45.0	21
GCGCAAA	20	7.034085E-4	45.0	13
GTTCGTC	20	7.034085E-4	45.0	28
TGCGACG	50	2.1827873E-11	45.0	1
AACGCGA	210	0.0	45.0	30
GCGTAGT	20	7.034085E-4	45.0	1
TACGCTA	145	0.0	43.44828	31
TCGTAAG	65	0.0	41.53846	1
ATTGCGA	65	0.0	41.53846	29
CGGTATC	300	0.0	41.25	29
ACGGTAT	300	0.0	41.25	28
CGTATGG	160	0.0	40.78125	2
ACGTTAT	105	0.0	40.714287	28
ATATCGC	310	0.0	40.645164	31
ACGTTAG	50	1.0822987E-9	40.5	1
GATATCG	290	0.0	40.34483	30
TAACGCG	235	0.0	40.212765	29
ACGCTAA	235	0.0	40.212765	25
CTATCGT	90	0.0	40.0	25
>>END_MODULE