FastQCFastQC Report
Sat 14 Jan 2017
SRR2935064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3435269
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319600.930349268135916No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT146300.4258764015277988No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC119620.3482114501076917No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT90440.2632690482171847No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG89940.2618135581231048No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC77980.2269982350727119No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC75580.22001188262112809No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT73340.2134912869996498Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT60450.17596875237426823No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTC55140.16051144757513894No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC43600.12691873620377325No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGT36440.10607611805654811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT196250.043.3719751
CGTTATT38500.042.837661
CGTTCTG15650.038.386581
TAACGCG1350.038.3333321
CGTTTTC12800.037.968751
TACGGGA18900.037.54
TACGGGT3850.036.8181844
TAAGGGA35200.036.754264
CGTTTCT19150.036.6579671
CGTTATG3400.036.3970571
ACGCAAC3550.036.12676226
TTACGCG751.8189894E-1236.0000041
CTACGGG8750.035.742863
CTAACGC2050.035.12195229
TTACGGG11550.035.0649383
GTTATTT45600.035.032892
ACGGGTA4900.034.897965
CGTTAGG4600.034.728262
GTTTTTT246800.034.1420172
TATACGG3300.034.0909082
Produced by FastQC (version 0.11.5)