FastQCFastQC Report
Sat 14 Jan 2017
SRR2935063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870472
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC68810.23971667377351183No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT67450.2349787770094953No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC53360.18589277303523602No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG39150.13638871934650468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGC302.166642E-645.00000415
CGTATCA207.035054E-445.045
CGTATGG4350.040.862072
CGAGATA19200.037.38281619
TATGGGA16150.037.1981434
TTACACG16500.036.95454834
TCGATAG1100.036.818181
TAACACG552.752131E-936.818181
TACACGC16800.036.56249635
ACGGGTA3100.036.290325
CGAATAT2550.036.1764714
TTAGACG1250.036.01
CGCATCG504.883259E-836.021
CGTCGTT250.002107633336.018
GCGTATG3000.036.01
TCGCGAT250.002107633336.025
ACACGCG16650.035.6756836
TATTACG950.035.5263141
GTCTTAC16850.035.38575431
TACGCAT2500.035.128
Produced by FastQC (version 0.11.5)