##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935063.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2870472
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.057525730959924	33.0	31.0	34.0	30.0	34.0
2	32.378944299056045	34.0	31.0	34.0	30.0	34.0
3	32.44024397381337	34.0	31.0	34.0	30.0	34.0
4	35.94037113060152	37.0	35.0	37.0	35.0	37.0
5	30.428402715650947	37.0	35.0	37.0	0.0	37.0
6	32.89184461649513	37.0	35.0	37.0	17.0	37.0
7	35.08261428782444	37.0	35.0	37.0	32.0	37.0
8	35.65531940391685	37.0	35.0	37.0	35.0	37.0
9	37.550329353500054	39.0	37.0	39.0	35.0	39.0
10	37.435362895022145	39.0	37.0	39.0	35.0	39.0
11	37.303766767277295	39.0	37.0	39.0	34.0	39.0
12	37.06177416118325	39.0	37.0	39.0	34.0	39.0
13	36.9721373349052	39.0	37.0	39.0	33.0	39.0
14	37.95203053713814	40.0	37.0	41.0	33.0	41.0
15	38.12404266615386	40.0	37.0	41.0	33.0	41.0
16	38.27693738172677	40.0	37.0	41.0	33.0	41.0
17	38.30471817875248	40.0	37.0	41.0	34.0	41.0
18	38.26371412088326	40.0	37.0	41.0	34.0	41.0
19	38.25932703750463	40.0	37.0	41.0	34.0	41.0
20	38.17065834469035	40.0	37.0	41.0	34.0	41.0
21	38.119059861932115	40.0	37.0	41.0	34.0	41.0
22	38.15484909798806	40.0	36.0	41.0	34.0	41.0
23	38.10504544200396	40.0	36.0	41.0	34.0	41.0
24	38.0710743738312	40.0	36.0	41.0	34.0	41.0
25	37.99464234453428	40.0	36.0	41.0	34.0	41.0
26	37.92851872444671	40.0	36.0	41.0	34.0	41.0
27	37.89699742760076	40.0	36.0	41.0	34.0	41.0
28	37.84587099264511	40.0	36.0	41.0	34.0	41.0
29	37.803080120621274	40.0	36.0	41.0	33.0	41.0
30	37.68207737264115	40.0	35.0	41.0	33.0	41.0
31	33.07180003846057	38.0	33.0	41.0	9.0	41.0
32	33.95488372643941	38.0	33.0	41.0	16.0	41.0
33	35.88246114227904	38.0	34.0	41.0	29.0	41.0
34	36.69734106446605	38.0	35.0	41.0	31.0	41.0
35	36.8348532924202	39.0	35.0	41.0	31.0	41.0
36	36.97875053301338	39.0	35.0	41.0	32.0	41.0
37	37.120229704383114	39.0	35.0	41.0	32.0	41.0
38	37.09581281405985	39.0	35.0	41.0	32.0	41.0
39	37.071765200984366	39.0	35.0	41.0	32.0	41.0
40	37.02033602836049	39.0	35.0	41.0	32.0	41.0
41	36.921555409702655	39.0	35.0	41.0	32.0	41.0
42	36.83351553333389	39.0	35.0	41.0	32.0	41.0
43	36.84669280870881	39.0	35.0	41.0	32.0	41.0
44	36.795320421171155	39.0	35.0	41.0	32.0	41.0
45	36.75011322179767	39.0	35.0	41.0	32.0	41.0
46	36.709246423584695	39.0	35.0	41.0	32.0	41.0
47	36.608613496316984	38.0	35.0	41.0	32.0	41.0
48	36.579889997185134	38.0	35.0	40.0	32.0	41.0
49	36.54420074468589	38.0	35.0	40.0	32.0	41.0
50	36.35725901524209	38.0	35.0	40.0	31.0	41.0
51	35.73050494831512	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	14.0
10	35.0
11	26.0
12	36.0
13	54.0
14	70.0
15	101.0
16	192.0
17	329.0
18	574.0
19	1034.0
20	1717.0
21	2785.0
22	4531.0
23	7254.0
24	10167.0
25	13494.0
26	17221.0
27	21292.0
28	26367.0
29	33117.0
30	45337.0
31	65433.0
32	95980.0
33	116756.0
34	221222.0
35	270302.0
36	330911.0
37	344254.0
38	492611.0
39	746499.0
40	752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61542073916763	21.42145960664309	25.242329484488963	11.720790169700313
2	31.22702468444214	24.135194490662162	25.943398855658582	18.694381969237114
3	32.291936657107264	23.945190895434617	25.994609945681407	17.76826250177671
4	28.794219208548284	25.80202837721462	25.792587421162793	19.611164993074308
5	22.68090404644254	39.04389243302147	21.103637311215717	17.17156620932028
6	27.515091594692443	33.209346755516165	23.74327288334462	15.532288766446772
7	80.66412074390553	6.326485679010281	7.774157002750767	5.2352365743334195
8	82.06082483995664	4.9217689634317985	7.597496160910122	5.419910035701445
9	76.33608688745267	6.6136161579001636	10.13247995451619	6.91781700013099
10	44.07762904497936	23.69742676465752	16.639179897940128	15.585764292422988
11	32.68486855123478	23.687080034224337	23.305644507244804	20.32240690729608
12	28.56258482925456	21.405295017683503	28.883368310159447	21.14875184290249
13	26.578102834655766	22.477627372780503	30.952470534462623	19.991799258101107
14	21.195817273256804	24.807592618914242	32.05789849195533	21.938691615873626
15	20.46565860945517	23.76933131554671	34.08585765685922	21.679152418138898
16	25.736812621756975	23.844545426675474	28.272075115172697	22.14656683639485
17	25.850208606807524	23.711396592616126	26.473067843894665	23.965326956681686
18	26.022932813836885	22.474213300112318	29.377712097522636	22.125141788528158
19	25.40439342379929	25.198678126802836	26.496339278000274	22.900589171397595
20	24.964221911936434	27.387830294111907	26.953650828156483	20.69429696579517
21	23.628448561769634	26.121383521595053	29.758834087216318	20.491333829418995
22	23.15483307274901	22.830426494318704	29.815410148574866	24.199330284357416
23	22.3278959000471	24.57780462585944	29.594017987285714	23.500281486807744
24	23.026038923215417	24.981849674896672	28.424280048716728	23.567831353171186
25	24.06747043691769	26.341835071026647	25.468668567399366	24.122025924656292
26	21.8648361663169	25.874176790437254	26.419487805489826	25.84149923775602
27	22.704070968119527	24.31774286598162	28.420169226524415	24.55801693937443
28	20.032768130119365	25.581402640401997	27.515056757216232	26.8707724722624
29	22.012303203096913	26.29177361771862	26.613044823290387	25.082878355894085
30	25.018812237151245	24.607381643158337	26.69756054056615	23.67624557912427
31	26.00659403749627	26.346781992647898	24.221556594176846	23.42506737567898
32	24.78449537218966	26.006977249734536	24.891132886856237	24.317394491219563
33	24.866572466131007	25.937128109941497	24.88075131894685	24.315548104980643
34	21.616653985825327	25.360358853874903	27.32052429008191	25.702462870217857
35	21.383312570197514	27.623227120835875	26.29299292938583	24.70046737958078
36	23.657781716735087	28.938550872469754	23.967521717682665	23.436145693112493
37	22.508493376699022	27.10017725307894	25.435050402860576	24.956278967361463
38	22.30072266860642	26.610884899765612	25.723191168560433	25.36520126306754
39	22.1195677923352	25.95621904690239	26.0740742289073	25.85013893185511
40	23.443287375734723	24.20863189050442	27.6030562221126	24.745024511648257
41	20.84747734867297	25.154608719402244	25.17836787817474	28.819546053750045
42	21.673508746993527	25.235884551390853	27.25994191895967	25.830664782655955
43	21.89942978018946	24.035524471236787	28.330427887817756	25.734617860756
44	22.296960221176167	23.616499307430974	27.21597702398769	26.870563447405164
45	21.58718148095505	23.36058320722167	26.43708073097386	28.61515458084942
46	22.721733568555972	24.13791181380623	27.013292587421162	26.127062030216635
47	19.48101218196868	25.49629468603073	29.211781198353442	25.81091193364715
48	20.02813474578397	26.019518741168703	27.98052027680465	25.971826236242677
49	23.049832919464116	23.493035291756893	28.652848730104317	24.80428305867467
50	20.688200407459124	24.11579001641542	28.200797638855214	26.99521193727025
51	20.17615918218328	23.801904355799326	26.11504310092556	29.906893361091836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1450.0
1	2274.5
2	3099.0
3	4618.0
4	6137.0
5	5004.5
6	3872.0
7	3950.5
8	4029.0
9	4425.0
10	4821.0
11	5159.0
12	5497.0
13	5630.5
14	5764.0
15	5883.5
16	6003.0
17	5858.0
18	5713.0
19	6037.0
20	6361.0
21	6792.0
22	7223.0
23	8942.0
24	10661.0
25	11774.0
26	16801.5
27	20716.0
28	23669.0
29	26622.0
30	31486.5
31	36351.0
32	40950.5
33	45550.0
34	53495.5
35	61441.0
36	68599.5
37	75758.0
38	81619.0
39	87480.0
40	97797.0
41	108114.0
42	120417.5
43	132721.0
44	147556.5
45	162392.0
46	194853.5
47	227315.0
48	258486.0
49	289657.0
50	289315.5
51	288974.0
52	257626.0
53	226278.0
54	207939.0
55	189600.0
56	176969.0
57	164338.0
58	155798.5
59	147259.0
60	135406.0
61	123553.0
62	113300.5
63	103048.0
64	92641.0
65	82234.0
66	69557.0
67	56880.0
68	50210.0
69	43540.0
70	36633.5
71	29727.0
72	25720.0
73	21713.0
74	18382.5
75	12369.0
76	9686.0
77	7775.0
78	5864.0
79	4283.0
80	2702.0
81	2126.0
82	1550.0
83	1061.0
84	572.0
85	354.5
86	137.0
87	109.0
88	81.0
89	62.5
90	44.0
91	24.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2870472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.97563236476055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.10120676964104	16.087938647406954
2	13.196550282420752	6.327912761087939
3	5.815671578164921	4.183032111368068
4	3.0192828029537644	2.8955685795545287
5	1.7974202167321787	2.1547143160679476
6	1.1418713305202914	1.6426252337048692
7	0.7817587854371776	1.3120212866294443
8	0.5767928953625416	1.106317952785448
9	0.4691973195320905	1.0124372195669238
>10	4.086722610154942	23.117387818249163
>50	1.7857905533214768	29.844070812651108
>100	0.21762894149372544	7.257224715858844
>500	0.00651994468716152	1.047264479049892
>1k	0.0030969737264017225	1.270450069013842
>5k	4.88995851537114E-4	0.7410339970051516
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	6881	0.23971667377351183	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	6745	0.2349787770094953	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	5336	0.18589277303523602	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3915	0.13638871934650468	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24647514415747654	0.0
2	0.0	0.0	0.0	0.8508008439030236	0.0
3	3.483747620600375E-5	0.0	0.0	1.2289616481191943	0.0
4	3.483747620600375E-5	0.0	0.0	1.707976945951746	0.0
5	6.96749524120075E-5	0.0	0.0	2.972403144848652	0.0
6	6.96749524120075E-5	0.0	0.0	3.8926002413540353	0.0
7	6.96749524120075E-5	0.0	0.0	4.679787853704896	0.0
8	6.96749524120075E-5	0.0	0.0	6.072276615135072	0.0
9	6.96749524120075E-5	0.0	0.0	6.573831760072908	0.0
10	6.96749524120075E-5	0.0	0.0	7.646059602741292	0.0
11	6.96749524120075E-5	0.0	0.0	9.303278345860889	0.0
12	6.96749524120075E-5	0.0	0.0	10.652115749604944	0.0
13	6.96749524120075E-5	0.0	0.0	11.193072080131769	0.0
14	6.96749524120075E-5	0.0	0.0	11.439651736717863	0.0
15	6.96749524120075E-5	0.0	0.0	11.87442343976879	0.0
16	6.96749524120075E-5	0.0	0.0	12.678472390603357	0.0
17	6.96749524120075E-5	0.0	0.0	13.62420535716774	0.0
18	6.96749524120075E-5	0.0	0.0	14.643933123193676	0.0
19	6.96749524120075E-5	0.0	0.0	15.326712819355144	0.0
20	6.96749524120075E-5	0.0	0.0	15.962740622448155	0.0
21	6.96749524120075E-5	0.0	0.0	16.687847852199916	0.0
22	6.96749524120075E-5	0.0	0.0	17.44545844725188	0.0
23	6.96749524120075E-5	0.0	0.0	18.121131298267322	0.0
24	6.96749524120075E-5	0.0	0.0	18.69462583157056	0.0
25	6.96749524120075E-5	0.0	0.0	19.223458720377693	0.0
26	6.96749524120075E-5	0.0	0.0	19.71508518459682	0.0
27	6.96749524120075E-5	0.0	0.0	20.261894211126254	0.0
28	6.96749524120075E-5	0.0	0.0	20.77146197559147	0.0
29	6.96749524120075E-5	0.0	0.0	21.35084404237352	0.0
30	6.96749524120075E-5	0.0	0.0	22.01780752433746	0.0
31	6.96749524120075E-5	0.0	0.0	22.534447296472496	0.0
32	6.96749524120075E-5	0.0	0.0	23.095260988436745	0.0
33	6.96749524120075E-5	0.0	0.0	23.69098183155941	0.0
34	6.96749524120075E-5	0.0	0.0	24.29917449116382	0.0
35	6.96749524120075E-5	0.0	0.0	24.97244355632105	0.0
36	6.96749524120075E-5	0.0	0.0	25.559246005534977	0.0
37	6.96749524120075E-5	0.0	0.0	26.17973629424011	0.0
38	6.96749524120075E-5	0.0	0.0	26.893416831796305	0.0
39	6.96749524120075E-5	0.0	0.0	28.04329740892787	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCGC	30	2.166642E-6	45.000004	15
CGTATCA	20	7.035054E-4	45.0	45
CGTATGG	435	0.0	40.86207	2
CGAGATA	1920	0.0	37.382816	19
TATGGGA	1615	0.0	37.198143	4
TTACACG	1650	0.0	36.954548	34
TCGATAG	110	0.0	36.81818	1
TAACACG	55	2.752131E-9	36.81818	1
TACACGC	1680	0.0	36.562496	35
ACGGGTA	310	0.0	36.29032	5
CGAATAT	255	0.0	36.17647	14
TTAGACG	125	0.0	36.0	1
CGCATCG	50	4.883259E-8	36.0	21
CGTCGTT	25	0.0021076333	36.0	18
GCGTATG	300	0.0	36.0	1
TCGCGAT	25	0.0021076333	36.0	25
ACACGCG	1665	0.0	35.67568	36
TATTACG	95	0.0	35.526314	1
GTCTTAC	1685	0.0	35.385754	31
TACGCAT	250	0.0	35.1	28
>>END_MODULE