##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935062.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1423318
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.983635420896807	33.0	31.0	34.0	30.0	34.0
2	32.29857277150995	34.0	31.0	34.0	30.0	34.0
3	32.36081465983006	34.0	31.0	34.0	30.0	34.0
4	35.88884353320902	37.0	35.0	37.0	35.0	37.0
5	30.363258948457055	37.0	35.0	37.0	0.0	37.0
6	32.843624544901424	37.0	35.0	37.0	17.0	37.0
7	35.036105775378374	37.0	35.0	37.0	32.0	37.0
8	35.58165427543248	37.0	35.0	37.0	35.0	37.0
9	37.430168802755254	39.0	37.0	39.0	35.0	39.0
10	37.37293633608231	39.0	37.0	39.0	34.0	39.0
11	37.28557637857457	39.0	37.0	39.0	34.0	39.0
12	37.096173869788764	39.0	37.0	39.0	34.0	39.0
13	37.053854444333595	39.0	37.0	39.0	33.0	39.0
14	38.219648736262734	40.0	37.0	41.0	33.0	41.0
15	38.33159701486245	40.0	37.0	41.0	34.0	41.0
16	38.40634137979004	40.0	37.0	41.0	34.0	41.0
17	38.38123595710867	40.0	37.0	41.0	34.0	41.0
18	38.32727261230449	40.0	37.0	41.0	34.0	41.0
19	38.31003893718761	40.0	37.0	41.0	34.0	41.0
20	38.24398201947843	40.0	37.0	41.0	34.0	41.0
21	38.178553211580265	40.0	37.0	41.0	34.0	41.0
22	38.14971777213525	40.0	37.0	41.0	34.0	41.0
23	38.08475899271983	40.0	36.0	41.0	34.0	41.0
24	38.07504366557579	40.0	36.0	41.0	34.0	41.0
25	38.013220517129696	40.0	36.0	41.0	34.0	41.0
26	37.921873397230975	40.0	36.0	41.0	34.0	41.0
27	37.86868500222719	40.0	36.0	41.0	33.0	41.0
28	37.78552157704743	40.0	36.0	41.0	33.0	41.0
29	37.784060202990474	40.0	36.0	41.0	33.0	41.0
30	37.66227505026986	40.0	36.0	41.0	33.0	41.0
31	33.044419448078365	38.0	33.0	41.0	9.0	41.0
32	33.92117362388447	38.0	33.0	41.0	16.0	41.0
33	35.840545120626594	38.0	34.0	41.0	29.0	41.0
34	36.69534144864324	38.0	35.0	41.0	31.0	41.0
35	36.86765993263628	39.0	35.0	41.0	32.0	41.0
36	37.01232612810349	39.0	35.0	41.0	32.0	41.0
37	37.08363485882986	39.0	35.0	41.0	32.0	41.0
38	37.045010320954276	39.0	35.0	41.0	32.0	41.0
39	37.098020259703034	39.0	35.0	41.0	32.0	41.0
40	37.06780845882649	39.0	35.0	41.0	32.0	41.0
41	36.98987295881876	39.0	35.0	41.0	32.0	41.0
42	36.89375037763873	39.0	35.0	41.0	32.0	41.0
43	36.88282520139561	39.0	35.0	41.0	32.0	41.0
44	36.82235593170324	39.0	35.0	41.0	32.0	41.0
45	36.807726734292686	39.0	35.0	41.0	32.0	41.0
46	36.73724775489384	39.0	35.0	41.0	32.0	41.0
47	36.64225352310587	39.0	35.0	41.0	31.0	41.0
48	36.651985009674576	39.0	35.0	41.0	32.0	41.0
49	36.63941227469898	39.0	35.0	41.0	32.0	41.0
50	36.45646580735998	39.0	35.0	41.0	31.0	41.0
51	35.86894706594029	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	16.0
11	13.0
12	15.0
13	21.0
14	18.0
15	47.0
16	94.0
17	184.0
18	331.0
19	607.0
20	977.0
21	1766.0
22	2828.0
23	4580.0
24	6243.0
25	7484.0
26	8817.0
27	10140.0
28	12604.0
29	15817.0
30	20957.0
31	29371.0
32	46486.0
33	54178.0
34	99235.0
35	142791.0
36	166839.0
37	166208.0
38	248759.0
39	375556.0
40	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06523067930006	22.14066006331684	24.65478550822796	11.139323749155142
2	31.0012239007727	24.996100660569176	25.786296526847828	18.216378911810292
3	31.499917797709298	25.678590448515372	25.715335575043664	17.10615617873167
4	28.506419507095394	26.19175756928529	26.440472192440478	18.861350731178835
5	22.19496978187587	39.50916098861955	20.561462723017627	17.734406506486955
6	25.806952487075975	34.471635994205094	23.778944691207446	15.94246682751149
7	78.33618348113353	7.097781381251414	8.968129399052074	5.597905738562991
8	77.98749120013939	6.392598140401512	9.05419590000267	6.565714759456425
9	71.45817027537065	8.30474988723532	12.223199594187665	8.013880243206367
10	40.502895347350346	24.878277377227015	18.729616290948332	15.889210984474305
11	30.675576364522893	25.03425095445993	23.76854645272525	20.521626228291918
12	27.40336312756531	22.42759523873091	29.622614201464465	20.546427432239316
13	24.212860372734696	24.804857382538547	30.422435464175962	20.559846780550796
14	19.876232858714637	28.882934101866205	28.84878853495846	22.392044504460703
15	18.714299966697535	26.822537198293002	33.92769570819733	20.53546712681214
16	20.761699072167993	26.524571459083635	30.629627391770498	22.084102076977878
17	20.899194698584576	26.207495443744826	27.17396955564392	25.719340302026673
18	20.898070564694606	26.0536296175556	30.306930706981856	22.741369110767938
19	22.870995799954756	27.599032682787684	27.346313332649487	22.183658184608078
20	24.822492232937403	27.017644686570392	27.838683976455016	20.32117910403719
21	22.441225362146756	28.087890408187064	28.184495664356103	21.286388565310073
22	20.777577463363777	26.51002797688219	26.105691068334696	26.60670349141935
23	21.501168396661885	27.16026917385995	27.847114980629765	23.491447448848398
24	22.62150833475021	24.508788619268497	28.746984159548322	24.122718886432967
25	20.828093230044164	27.493293838762668	26.3189252155878	25.359687715605368
26	19.856982065848953	28.077843461545488	28.757171622926148	23.30800284967941
27	23.099194979618048	27.159636848546846	27.564816857511815	22.17635131432329
28	19.76262507745985	28.26002341008826	28.338150715440964	23.63920079701093
29	22.433777975125725	25.3662217438408	28.451828755063875	23.7481715259696
30	25.37535533169678	26.47405569240324	25.810043855273385	22.340545120626594
31	25.867234166925453	29.11907247712739	22.68417879911587	22.329514556831292
32	24.584597398473147	28.10833559331084	25.458681756290584	21.848385251925432
33	25.798802516373716	26.246840129893673	25.322591297236457	22.631766056496158
34	21.798712585662518	26.352930265759305	27.1398942471043	24.70846290147388
35	23.972787528858625	25.522827646386826	26.842209541367424	23.66217528338713
36	24.123351211746076	27.049541985698205	27.64329545470513	21.183811347850586
37	23.36413928580964	28.308501684093084	27.45774310449246	20.86961592560482
38	23.568450620311133	28.437355531230544	24.924507383451903	23.069686465006413
39	25.387650546118294	23.93035147451237	27.613505906621004	23.068492072748327
40	24.1828600495462	23.41324988512757	28.81225418353453	23.5916358817917
41	20.325675639597055	25.952176533986083	27.024459748278318	26.697688078138547
42	22.388461327686436	25.01654584569295	29.17317142058205	23.421821406038564
43	24.233656849699084	23.58594495397374	29.388724093983214	22.791674102343958
44	22.84795105521043	24.733193847053155	27.13820804626935	25.280647051467064
45	23.287557664555635	22.63155528139179	27.371044278228755	26.709842775823816
46	24.67157725820934	23.83381647671146	27.93191683095415	23.56268943412505
47	21.026362344887087	23.911662748591674	30.50154638668239	24.560428519838855
48	20.949850982001212	23.284115004517613	30.44906338569455	25.316970627786624
49	22.89888837209956	21.55948284220392	31.704720940787652	23.83690784490887
50	21.733091269835693	21.814450460122053	30.748715325738875	25.703742944303382
51	20.80434590161861	21.649062261560665	27.95643700142906	29.59015483539167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1753.0
1	2115.5
2	2478.0
3	2990.5
4	3503.0
5	3062.0
6	2621.0
7	2613.5
8	2606.0
9	2876.5
10	3147.0
11	3463.5
12	3780.0
13	3952.0
14	4124.0
15	4276.5
16	4429.0
17	4487.0
18	4545.0
19	4500.0
20	4455.0
21	4860.5
22	5266.0
23	5795.5
24	6325.0
25	7251.5
26	11225.5
27	14273.0
28	15608.5
29	16944.0
30	18792.0
31	20640.0
32	23881.0
33	27122.0
34	27743.5
35	28365.0
36	30442.0
37	32519.0
38	36540.0
39	40561.0
40	47732.5
41	54904.0
42	62639.0
43	70374.0
44	83761.5
45	97149.0
46	115703.0
47	134257.0
48	150569.5
49	166882.0
50	159322.0
51	151762.0
52	129523.5
53	107285.0
54	95919.0
55	84553.0
56	75199.0
57	65845.0
58	62248.5
59	58652.0
60	55736.5
61	52821.0
62	46696.5
63	40572.0
64	36031.5
65	31491.0
66	26823.5
67	22156.0
68	18871.5
69	15587.0
70	13509.0
71	11431.0
72	9625.0
73	7819.0
74	6240.0
75	4051.0
76	3441.0
77	2709.0
78	1977.0
79	1582.0
80	1187.0
81	839.5
82	492.0
83	405.0
84	318.0
85	168.5
86	19.0
87	15.0
88	11.0
89	18.0
90	25.0
91	19.0
92	13.0
93	6.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1423318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.545502529351715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.48025884828917	16.982716205609275
2	13.511323663921859	6.903071056632111
3	6.055653629290821	4.640841453117797
4	3.229071275338704	3.299529937264873
5	1.958640734484024	2.501723091842647
6	1.292340989392909	1.9808100007992873
7	0.8947500964325702	1.5999788596089137
8	0.640910488021263	1.3097904394268174
9	0.4669399507942467	1.0735394124662818
>10	3.8030522839654357	23.081926353863615
>50	1.4884896614614804	26.05731770382394
>100	0.1658356768299451	6.532555691121073
>500	0.00807439624924138	1.3719763202381807
>1k	0.003726644422726791	1.240443028983705
>5k	9.316611056816977E-4	1.4237804452014935
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT	6675	0.46897460722059303	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	6042	0.424501060198775	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	5230	0.3674512652829515	No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	2015	0.1415706117677146	No Hit
ACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	1691	0.11880690049588356	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC	1566	0.11002460448051665	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.025836812293528E-5	0.0	0.0	0.49497020342607906	0.0
2	7.025836812293528E-5	0.0	0.0	1.6079330128614968	0.0
3	7.025836812293528E-5	0.0	0.0	2.2570500759492957	0.0
4	7.025836812293528E-5	0.0	0.0	3.013732700633309	0.0
5	7.025836812293528E-5	0.0	0.0	4.900802210047227	0.0
6	7.025836812293528E-5	0.0	0.0	6.2325495778174655	0.0
7	7.025836812293528E-5	0.0	0.0	7.365114471959183	0.0
8	7.025836812293528E-5	0.0	0.0	9.341201333784861	0.0
9	7.025836812293528E-5	0.0	0.0	10.013644175089475	0.0
10	7.025836812293528E-5	0.0	0.0	11.46117733352631	0.0
11	7.025836812293528E-5	0.0	0.0	13.639327262073548	0.0
12	7.025836812293528E-5	0.0	0.0	15.4419462130037	0.0
13	7.025836812293528E-5	0.0	0.0	16.15015056368289	0.0
14	7.025836812293528E-5	0.0	0.0	16.446921910634167	0.0
15	7.025836812293528E-5	0.0	0.0	16.97287605440246	0.0
16	7.025836812293528E-5	0.0	0.0	18.084644471579786	0.0
17	7.025836812293528E-5	0.0	0.0	19.350348973314468	0.0
18	7.025836812293528E-5	0.0	0.0	20.95912508659344	0.0
19	7.025836812293528E-5	0.0	0.0	21.873678264449687	0.0
20	1.4051673624587057E-4	0.0	0.0	22.651508657938702	0.0
21	1.4051673624587057E-4	0.0	0.0	23.5006512950725	0.0
22	1.4051673624587057E-4	0.0	0.0	24.38155071459786	0.0
23	1.4051673624587057E-4	0.0	0.0	25.169990121673443	0.0
24	2.8103347249174113E-4	0.0	0.0	25.85065319204844	0.0
25	2.8103347249174113E-4	0.0	0.0	26.428809303332073	0.0
26	3.512918406146764E-4	0.0	0.0	26.985747387442583	0.0
27	3.512918406146764E-4	0.0	0.0	27.509523521799064	0.0
28	3.512918406146764E-4	0.0	0.0	28.065548247123974	0.0
29	3.512918406146764E-4	0.0	0.0	28.625437182695645	0.0
30	3.512918406146764E-4	0.0	0.0	29.244343147490582	0.0
31	3.512918406146764E-4	0.0	0.0	29.802405365491058	0.0
32	3.512918406146764E-4	0.0	0.0	30.467752111615255	0.0
33	3.512918406146764E-4	0.0	0.0	31.09501882221682	0.0
34	3.512918406146764E-4	0.0	0.0	31.691582625948662	0.0
35	3.512918406146764E-4	0.0	0.0	32.33915400493776	0.0
36	3.512918406146764E-4	0.0	0.0	32.963469864078164	0.0
37	3.512918406146764E-4	0.0	0.0	33.57612283411016	0.0
38	3.512918406146764E-4	0.0	0.0	34.18575469431287	0.0
39	3.512918406146764E-4	0.0	0.0	34.83908725948804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	20	7.0338545E-4	45.0	42
TCGCAAG	20	7.0338545E-4	45.0	1
GACGTAC	25	3.891276E-5	45.0	34
ACGATTC	20	7.0338545E-4	45.0	30
TACGCGC	20	7.0338545E-4	45.0	34
TCGTCAA	20	7.0338545E-4	45.0	11
AATCTCG	20	7.0338545E-4	45.0	18
TCGTAAT	20	7.0338545E-4	45.0	21
TATAGCG	50	2.1827873E-11	45.0	1
TACCGGA	20	7.0338545E-4	45.0	3
TAACGCG	30	2.1658689E-6	44.999996	1
TACGGGT	170	0.0	42.35294	4
TTAACGG	145	0.0	41.896553	2
GGTAACG	140	0.0	41.785713	1
TTCGAAG	55	6.184564E-11	40.909092	1
TATACGG	105	0.0	40.714287	2
TATCGAG	50	1.0822987E-9	40.5	1
GCGTAAG	150	0.0	40.5	1
GTTAACG	95	0.0	40.263157	1
ACACGAT	45	1.9288564E-8	40.0	22
>>END_MODULE