##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935061.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1557332
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82549449956721	33.0	31.0	34.0	30.0	34.0
2	32.15371930969119	34.0	31.0	34.0	30.0	34.0
3	32.2224708668415	34.0	31.0	34.0	30.0	34.0
4	35.776312950610404	37.0	35.0	37.0	35.0	37.0
5	30.304663360156987	37.0	35.0	37.0	0.0	37.0
6	32.76633434617666	37.0	35.0	37.0	17.0	37.0
7	34.9562097227823	37.0	35.0	37.0	32.0	37.0
8	35.50408133911073	37.0	35.0	37.0	33.0	37.0
9	37.35138043782572	39.0	37.0	39.0	34.0	39.0
10	37.28220379469503	39.0	37.0	39.0	34.0	39.0
11	37.16408126205587	39.0	37.0	39.0	34.0	39.0
12	37.02661795943318	39.0	37.0	39.0	33.0	39.0
13	36.982112998384416	39.0	37.0	39.0	33.0	39.0
14	38.11298746831119	40.0	37.0	41.0	33.0	41.0
15	38.20538716214654	40.0	37.0	41.0	33.0	41.0
16	38.28676801093152	40.0	37.0	41.0	34.0	41.0
17	38.261595472256396	40.0	37.0	41.0	34.0	41.0
18	38.19742418443852	40.0	37.0	41.0	34.0	41.0
19	38.17610053604498	40.0	37.0	41.0	34.0	41.0
20	38.09356450647646	40.0	37.0	41.0	34.0	41.0
21	38.04207773294326	40.0	37.0	41.0	34.0	41.0
22	38.02703983479438	40.0	36.0	41.0	34.0	41.0
23	37.92927262780191	40.0	36.0	41.0	33.0	41.0
24	37.91321054213231	40.0	36.0	41.0	33.0	41.0
25	37.8588040315103	40.0	36.0	41.0	33.0	41.0
26	37.817195048968365	40.0	36.0	41.0	33.0	41.0
27	37.773534480765825	40.0	36.0	41.0	33.0	41.0
28	37.70561126336581	40.0	36.0	41.0	33.0	41.0
29	37.71647086170451	40.0	36.0	41.0	33.0	41.0
30	37.5786916341538	40.0	36.0	41.0	33.0	41.0
31	33.02784891082955	38.0	33.0	41.0	9.0	41.0
32	33.892599009074495	38.0	33.0	41.0	16.0	41.0
33	35.8017802241269	38.0	34.0	40.0	29.0	41.0
34	36.64660136695323	38.0	35.0	40.0	31.0	41.0
35	36.789597850683094	39.0	35.0	40.0	31.0	41.0
36	36.918201128596856	39.0	35.0	40.0	31.0	41.0
37	37.037318953184034	39.0	35.0	41.0	32.0	41.0
38	36.99258796454449	39.0	35.0	41.0	32.0	41.0
39	36.99224699678681	39.0	35.0	41.0	32.0	41.0
40	36.99440774349978	39.0	35.0	41.0	32.0	41.0
41	36.887600075000066	39.0	35.0	41.0	32.0	41.0
42	36.77470764101682	39.0	35.0	41.0	31.0	41.0
43	36.720846935656624	39.0	35.0	41.0	31.0	41.0
44	36.655530098912756	39.0	35.0	41.0	31.0	41.0
45	36.63886120621679	39.0	35.0	41.0	31.0	41.0
46	36.56733760046028	39.0	35.0	41.0	31.0	41.0
47	36.43630324169798	39.0	35.0	40.0	31.0	41.0
48	36.43121376816247	39.0	35.0	40.0	31.0	41.0
49	36.413536098917895	39.0	35.0	40.0	31.0	41.0
50	36.21895267033619	38.0	35.0	40.0	31.0	41.0
51	35.575012264565295	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	10.0
11	9.0
12	17.0
13	22.0
14	23.0
15	52.0
16	113.0
17	191.0
18	371.0
19	721.0
20	1296.0
21	2165.0
22	3523.0
23	5342.0
24	7486.0
25	9115.0
26	10579.0
27	12249.0
28	14883.0
29	19099.0
30	25070.0
31	34692.0
32	51933.0
33	62540.0
34	108736.0
35	154197.0
36	186495.0
37	183802.0
38	275841.0
39	386515.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.682140994983726	21.46401666439783	25.79565564696545	12.05818669365299
2	30.284037058250906	25.92921740515189	25.803296920630924	17.98344861596628
3	30.414452409633913	25.893900594092973	26.460574880629178	17.231072115643933
4	27.078940136078884	26.14753950987972	27.71759650479153	19.05592384924987
5	22.22461234983934	38.863903136903375	21.26842574351519	17.643058769742098
6	25.75321126131101	33.874408282883806	24.20402329111583	16.168357164689354
7	75.7868585503926	6.979051351927527	10.927535040697808	6.306555056982069
8	75.32054821964745	6.511071499205051	10.470150231293006	7.698230049854494
9	69.55909208826377	7.784916767908191	13.229870059820255	9.426121084007777
10	40.69697405562847	22.861406559423425	19.18524759010924	17.256371794838866
11	28.411282886372334	24.59250821276388	25.460595428591976	21.53561347227181
12	24.481806063190124	22.137797207018156	30.556811264393207	22.823585465398516
13	23.166929081274898	23.526775279773357	33.55000732021175	19.756288318739998
14	19.03730225796426	28.53431381362484	29.831660814778093	22.5967231136328
15	17.791967287643224	25.34032563384044	35.03389129613981	21.833815782376526
16	19.19038458080872	26.727762609385795	30.025839063218374	24.056013746587112
17	19.60571027886154	25.78268474544927	28.211325523395143	26.40027945229405
18	20.207444526921684	24.984332178366593	31.069482936201144	23.738740358510583
19	20.86530039837363	26.7129937611248	29.016741452689597	23.404964387811976
20	22.445246100381937	27.01928683158119	28.69856909124066	21.836897976796212
21	21.340472038075376	28.90180128578877	28.930696858473336	20.827029817662517
22	20.26459354845338	25.374358197224485	27.951393794001532	26.409654460320596
23	19.584905466528653	26.924059866489614	27.89064887898021	25.600385788001528
24	21.561105788617972	24.907598379793132	28.064150739855087	25.467145091733812
25	20.075102804026372	27.960190890574392	26.179453064600228	25.785253240799005
26	20.567611787338862	26.902677142703034	27.433649343877864	25.096061726080244
27	21.94419687003157	25.863977623268514	26.749980094161042	25.441845412538882
28	18.27651393537152	24.983304780226696	30.558288149219305	26.181893135182477
29	21.876452805182193	23.112284342709195	28.500473887392026	26.510788964716582
30	22.047257745939852	23.782083717537432	27.508970470008965	26.661688066513754
31	24.48655777958714	25.60475223009609	25.88869939100975	24.01999059930702
32	23.99565410586824	24.584995363865893	27.540370325659524	23.87898020460634
33	21.122149933347544	22.893898025597625	27.960897226795574	28.023054814259257
34	21.986641255686006	21.454834293522513	31.94238608081	24.61613836998148
35	23.32065352795679	21.800810617132377	28.61554247906034	26.262993375850492
36	22.875469071463247	21.847878294416347	30.85604097263782	24.420611661482585
37	20.804876545271014	22.19597362668975	34.10223382040567	22.896916007633568
38	21.23111834856023	23.223050704666697	30.465180192791262	25.08065075398181
39	21.762732673572494	21.162218460803476	32.64223685123018	24.43281201439385
40	23.28437353114172	20.087367369321377	31.345660398681847	25.282598700855054
41	22.445824011835626	21.653892683127296	28.888637747121358	27.01164555791572
42	24.216287856410837	20.20834350029409	31.170296378678408	24.405072264616663
43	22.382253751929582	19.669858450221277	32.27596941435738	25.671918383491764
44	20.79961112980405	20.613780491250424	30.571580112654207	28.015028266291324
45	21.44719301985704	19.031523143427346	30.43904575260766	29.08223808410795
46	24.59507670811362	20.223882897160014	30.223613205148293	24.957427189578073
47	19.58041059966661	20.276537051829667	34.80529521001302	25.3377571384907
48	20.265942008511995	19.39329571343811	33.41021696080219	26.9305453172477
49	22.278807601718835	17.53678727464664	35.68102369950659	24.50338142412793
50	21.21249675727462	17.78965564182846	33.507883996476025	27.489963604420893
51	19.99329622713718	17.882378323954047	30.31492321483152	31.809402234077254
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1944.0
1	2290.5
2	2637.0
3	3202.5
4	3768.0
5	3276.5
6	2785.0
7	2856.0
8	2927.0
9	3267.0
10	3607.0
11	3924.0
12	4241.0
13	4407.0
14	4573.0
15	4560.0
16	4547.0
17	4557.0
18	4567.0
19	4674.0
20	4781.0
21	5095.5
22	5410.0
23	5511.0
24	5612.0
25	6402.0
26	7939.0
27	8686.0
28	10396.0
29	12106.0
30	14378.0
31	16650.0
32	18869.5
33	21089.0
34	24484.0
35	27879.0
36	30542.5
37	33206.0
38	36550.5
39	39895.0
40	46680.0
41	53465.0
42	63344.5
43	73224.0
44	89916.0
45	106608.0
46	138040.0
47	169472.0
48	185964.5
49	202457.0
50	196582.0
51	190707.0
52	160495.0
53	130283.0
54	113414.5
55	96546.0
56	85131.5
57	73717.0
58	67170.5
59	60624.0
60	55002.0
61	49380.0
62	44366.0
63	39352.0
64	34847.0
65	30342.0
66	25478.5
67	20615.0
68	17299.5
69	13984.0
70	12738.5
71	11493.0
72	9373.0
73	7253.0
74	5934.0
75	3723.0
76	2831.0
77	2078.5
78	1326.0
79	1004.0
80	682.0
81	430.0
82	178.0
83	109.0
84	40.0
85	36.5
86	33.0
87	17.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1557332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.467058886574137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.13286807468376	18.164753817453775
2	13.89422397645646	7.632669362891596
3	6.153719598237285	5.070737357287465
4	3.3191232295668964	3.646662127932402
5	2.0230481785221475	2.7783591724922188
6	1.2978658035855524	2.138913387237314
7	0.8621554803125132	1.6576612742988488
8	0.6154667272105311	1.3524048671214974
9	0.4674932284680764	1.1556597631866579
>10	4.063710828546528	27.11465346046741
>50	1.0695176134438744	19.094249022458545
>100	0.08722700831067186	4.317657870638338
>500	0.008095919852449996	1.5616190343663023
>1k	0.004700856688519352	2.655684882421187
>5k	7.834761147532254E-4	1.6583145997464483
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	8422	0.5407966958875821	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	8110	0.5207624321596166	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	6586	0.42290275933455423	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	4645	0.29826652248846103	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	3923	0.25190518142566903	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	3229	0.2073417871076944	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	2942	0.1889128329733159	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	2467	0.15841195069516328	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	2303	0.14788111976123267	No Hit
TGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCTT	2114	0.1357449792337151	Illumina Single End Adapter 1 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTG	1678	0.10774837992155814	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2842476748695846E-4	0.0	0.0	0.5646194902564129	0.0
2	1.926371512304377E-4	0.0	0.0	1.9114742392758899	0.0
3	1.926371512304377E-4	0.0	0.0	2.694415834260132	0.0
4	1.926371512304377E-4	0.0	0.0	3.709485196477052	0.0
5	1.926371512304377E-4	0.0	0.0	6.233352939514503	0.0
6	1.926371512304377E-4	0.0	0.0	7.8838680512568935	0.0
7	1.926371512304377E-4	0.0	0.0	9.400179280975411	0.0
8	1.926371512304377E-4	0.0	0.0	11.953071021464917	0.0
9	1.926371512304377E-4	0.0	0.0	12.876445099696147	0.0
10	1.926371512304377E-4	0.0	0.0	14.955963147228722	0.0
11	1.926371512304377E-4	0.0	0.0	17.66450570591242	0.0
12	2.568495349739169E-4	0.0	0.0	20.198390580813854	0.0
13	2.568495349739169E-4	0.0	0.0	21.068211531003023	0.0
14	2.568495349739169E-4	0.0	0.0	21.415407889904014	0.0
15	2.568495349739169E-4	0.0	0.0	22.074676433798317	0.0
16	2.568495349739169E-4	0.0	0.0	23.33747717249758	0.0
17	2.568495349739169E-4	0.0	0.0	24.811793503247863	0.0
18	3.2106191871739614E-4	0.0	0.0	26.57243285311032	0.0
19	3.2106191871739614E-4	0.0	0.0	27.70546036426401	0.0
20	3.2106191871739614E-4	0.0	0.0	28.61772570010762	0.0
21	3.2106191871739614E-4	0.0	0.0	29.57352703212931	0.0
22	3.2106191871739614E-4	0.0	0.0	30.499341180942793	0.0
23	3.2106191871739614E-4	0.0	0.0	31.38052772305456	0.0
24	3.2106191871739614E-4	0.0	0.0	32.13014309087593	0.0
25	3.2106191871739614E-4	0.0	0.0	32.79852979326181	0.0
26	3.2106191871739614E-4	0.0	0.0	33.39185221905156	0.0
27	3.2106191871739614E-4	0.0	0.0	33.96809415076554	0.0
28	3.852743024608754E-4	0.0	0.0	34.566039868184816	0.0
29	3.852743024608754E-4	0.0	0.0	35.189798963868974	0.0
30	3.852743024608754E-4	0.0	0.0	35.83417023473479	0.0
31	3.852743024608754E-4	0.0	0.0	36.43628975709739	0.0
32	3.852743024608754E-4	0.0	0.0	37.07982626697454	0.0
33	4.4948668620435465E-4	0.0	0.0	37.70615385800844	0.0
34	4.4948668620435465E-4	0.0	0.0	38.3303624403788	0.0
35	4.4948668620435465E-4	0.0	0.0	38.99521746165878	0.0
36	5.136990699478338E-4	0.0	0.0	39.63169060932415	0.0
37	5.779114536913131E-4	0.0	0.0	40.21486747848243	0.0
38	5.779114536913131E-4	0.0	0.0	40.83426013207203	0.0
39	5.779114536913131E-4	0.0	0.0	41.49417079980377	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTAT	40	6.8175723E-9	45.000004	23
AGATTCG	20	7.034058E-4	45.000004	1
AGTTACG	40	6.8175723E-9	45.000004	1
CGGTCGT	20	7.034058E-4	45.000004	21
AATCGAC	20	7.034058E-4	45.000004	37
TGCGTAA	20	7.034058E-4	45.000004	29
TACGTCA	25	3.891446E-5	45.0	32
TAGTACG	85	0.0	45.0	1
CGGGTAT	160	0.0	42.187504	6
GTACGTA	65	0.0	41.538464	12
AATCGTT	50	1.0822987E-9	40.5	22
TACGGGA	485	0.0	40.360825	4
TATACGG	130	0.0	39.807693	2
CGTCTAG	40	3.4592813E-7	39.375004	15
ACGCATC	275	0.0	39.272728	29
ATACGCA	275	0.0	39.272728	27
GCGATAT	110	0.0	38.863636	9
TACGGGT	140	0.0	38.571426	4
TACGCAT	280	0.0	38.571426	28
TATACGC	280	0.0	38.571426	26
>>END_MODULE