SRR2935059.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935059.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1045337
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	15641	1.496263884278467	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	13106	1.2537583573527007	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	12412	1.1873682841035953	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	4644	0.44425864577643376	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	4584	0.4385188699912086	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	3888	0.37193747088259577	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3763	0.35997960466337653	No Hit
TGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCTT	3512	0.33596820929518423	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC	2764	0.2644123378393762	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	2514	0.2404966054009377	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	2362	0.22595584007836705	No Hit
GAAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	2353	0.2250948737105833	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTT	2262	0.2163895471029917	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	2212	0.21160640061530397	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	2165	0.20711024291687752	No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1989	0.19027356728021683	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1987	0.1900822414207093	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	1616	0.15459129448206654	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	1514	0.14483367564718364	No Hit
TGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1382	0.1322061689196881	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1305	0.12484012332864902	No Hit
GACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	1199	0.1146998527747511	No Hit
GTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1188	0.11364756054745981	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	1157	0.11068200972509344	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTC	1133	0.10838609941100336	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG	1070	0.10235933483651685	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	55	1.8189894E-12	45.000004	43
TATCACG	30	2.1653123E-6	45.000004	1
CGCATGT	55	1.8189894E-12	45.000004	38
CGCGCAT	55	1.8189894E-12	45.000004	36
CGCCCTA	30	2.1653123E-6	45.000004	17
TCAACGG	30	2.1653123E-6	45.000004	2
ACGGTGT	30	2.1653123E-6	45.000004	22
CTATGCG	20	7.0329936E-4	45.0	1
AAGTACG	40	6.8139343E-9	45.0	1
TTAGGCG	70	0.0	45.0	1
AGGTACG	40	6.8139343E-9	45.0	1
GGTACAT	20	7.0329936E-4	45.0	8
TCGCAAG	45	3.8562575E-10	45.0	1
AATCACG	25	3.8905637E-5	45.0	29
GATCGAC	20	7.0329936E-4	45.0	23
GGCACGT	20	7.0329936E-4	45.0	11
CGGCCAA	20	7.0329936E-4	45.0	7
TAGCGTG	25	3.8905637E-5	45.0	1
GCTATTA	40	6.8139343E-9	45.0	18
ATAGCCG	20	7.0329936E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content