##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935059.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1045337
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.562812757990965	31.0	31.0	34.0	30.0	34.0
2	31.882119354810936	33.0	31.0	34.0	30.0	34.0
3	31.961360786043162	33.0	31.0	34.0	30.0	34.0
4	35.60669334386901	37.0	35.0	37.0	33.0	37.0
5	30.15426030074512	35.0	33.0	37.0	0.0	37.0
6	32.615074373144736	36.0	33.0	37.0	17.0	37.0
7	34.73746456884239	37.0	35.0	37.0	32.0	37.0
8	35.25034988716558	37.0	35.0	37.0	33.0	37.0
9	36.96891528760582	39.0	37.0	39.0	33.0	39.0
10	37.10047573174967	39.0	37.0	39.0	34.0	39.0
11	37.0557494855726	39.0	37.0	39.0	33.0	39.0
12	37.00506056898397	39.0	37.0	39.0	33.0	39.0
13	36.93462299717699	39.0	37.0	39.0	33.0	39.0
14	37.96404413122276	40.0	37.0	41.0	33.0	41.0
15	38.07788684414691	40.0	37.0	41.0	33.0	41.0
16	38.14048579548987	40.0	37.0	41.0	33.0	41.0
17	38.11705603073459	40.0	37.0	41.0	34.0	41.0
18	37.99716168087421	39.0	37.0	41.0	33.0	41.0
19	37.8846582489666	39.0	36.0	41.0	34.0	41.0
20	37.764408989636834	39.0	36.0	41.0	33.0	41.0
21	37.66436661095896	39.0	36.0	41.0	33.0	41.0
22	37.627723882346075	39.0	35.0	41.0	33.0	41.0
23	37.52878449724825	39.0	35.0	41.0	33.0	41.0
24	37.50482954300862	39.0	35.0	41.0	33.0	41.0
25	37.42643855522191	39.0	35.0	41.0	33.0	41.0
26	37.395250526863585	39.0	35.0	41.0	33.0	41.0
27	37.3661087285727	39.0	35.0	41.0	33.0	41.0
28	37.31641374982422	39.0	35.0	41.0	33.0	41.0
29	37.331626068913664	39.0	35.0	41.0	33.0	41.0
30	37.14252819904012	39.0	35.0	41.0	32.0	41.0
31	32.570159670996055	38.0	32.0	40.0	9.0	41.0
32	33.328031056013515	38.0	31.0	40.0	16.0	41.0
33	35.11255030674318	38.0	33.0	40.0	25.0	41.0
34	35.842028934209736	38.0	35.0	40.0	30.0	41.0
35	35.95282573945053	38.0	35.0	40.0	30.0	41.0
36	35.986470391844925	39.0	35.0	40.0	30.0	41.0
37	35.930662551885185	39.0	35.0	40.0	30.0	41.0
38	35.8670993182103	39.0	35.0	40.0	29.0	41.0
39	35.88382598147774	39.0	35.0	40.0	29.0	41.0
40	35.78873511604392	39.0	35.0	40.0	29.0	41.0
41	35.69811936246397	39.0	35.0	40.0	28.0	41.0
42	35.55877004257957	39.0	35.0	40.0	27.0	41.0
43	35.45350446793714	39.0	35.0	40.0	26.0	41.0
44	35.33921979227751	38.0	35.0	40.0	26.0	41.0
45	35.44396687384069	39.0	35.0	40.0	26.0	41.0
46	35.420004266566664	39.0	35.0	40.0	26.0	41.0
47	35.30102636757333	39.0	35.0	40.0	25.0	41.0
48	35.31386815926347	39.0	35.0	40.0	25.0	41.0
49	35.31691693683472	39.0	35.0	40.0	25.0	41.0
50	35.0783957709332	38.0	35.0	40.0	24.0	41.0
51	34.5333906673159	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	10.0
11	6.0
12	7.0
13	10.0
14	27.0
15	52.0
16	134.0
17	300.0
18	655.0
19	1171.0
20	1910.0
21	3140.0
22	5313.0
23	8502.0
24	10968.0
25	11943.0
26	11993.0
27	12673.0
28	13884.0
29	15969.0
30	19775.0
31	25593.0
32	36046.0
33	44022.0
34	70501.0
35	100630.0
36	123321.0
37	117985.0
38	191634.0
39	217092.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.683722091536026	23.24896181805485	22.84105508558484	13.226261004824282
2	29.17996779985784	27.971075356559655	23.880145828570118	18.96881101501238
3	27.307748601647123	29.167244630200596	27.03778781388203	16.48721895427025
4	25.911739467750593	29.210197285660033	26.385079644172166	18.492983602417212
5	18.87726159123804	40.22214845547417	22.334711198398217	18.56587875488957
6	22.20853179405302	33.063595759071	26.944707783231625	17.783164663644353
7	64.88491271235975	11.3932636078126	14.81914444815404	8.902679231673613
8	60.24124277625301	10.49680629309017	16.668021891504843	12.593929039151966
9	54.37251336171971	12.398872325384062	20.3377475397886	12.89086677310762
10	30.531302345559375	26.994930821352348	24.9614239235768	17.512342909511478
11	21.279931734933328	28.414473035968307	27.97825007629119	22.327345152807183
12	18.876304961940505	23.449758307607976	35.742062129246364	21.931874601205163
13	19.12435893879199	27.648882609149013	35.83973398052494	17.38702447153406
14	14.01031437708605	33.61815376285351	29.946610518904432	22.424921341156008
15	11.424641048771832	29.921833820098207	38.10971964065177	20.54380549047819
16	11.740233054029465	32.60699659535633	30.76261530970395	24.89015504091025
17	12.023108337311317	31.786112995139366	27.142347396102885	29.048431271446436
18	13.611399960012896	29.77881774011635	32.4546055482586	24.15517675161216
19	14.231869722395743	30.953367191632935	29.506465379107404	25.308297706863907
20	16.316173635870538	32.73661986517267	29.621547883601174	21.32565861535562
21	15.892578182920914	34.1440128877099	30.649541726735013	19.313867202634174
22	13.936940909964921	30.727985329133094	25.786229703913666	29.548844056988322
23	14.32887193316605	31.143927747702417	27.666293262364196	26.860907056767342
24	19.4292366959172	27.68762609569928	26.619932136717633	26.263205071665887
25	14.067042494430027	32.00728568873005	24.171630775529806	29.754041041310124
26	13.439589338175153	29.658091122767104	28.920816923154923	27.981502615902816
27	18.66422024667643	28.783827607747547	29.55381852933552	22.998133616240505
28	12.335639128816831	26.891901846007553	33.203837614090006	27.568621411085616
29	13.292842403932895	24.352816364483417	30.892334242450044	31.462006989133645
30	17.036228508127042	24.91330546991066	31.414462513046033	26.63600350891626
31	19.541927627167123	27.532269497779183	29.34603864591036	23.57976422914333
32	18.980099240723327	25.110945082781917	29.20541413917234	26.703541537322412
33	20.09859021540422	22.324953579563335	29.756336951624213	27.820119253408233
34	17.69687670100647	19.36188999337056	35.57331272116074	27.367920584462237
35	21.746479843342385	18.531631426037727	30.443388113115677	29.27850061750421
36	24.491431949696604	18.596012577762004	32.48368707890374	24.42886839363765
37	20.585992842499596	19.909655929140555	40.6779823157508	18.82636891260904
38	21.811147983855925	22.033372969673895	32.24089456318872	23.914584483281466
39	25.837218045472415	17.86285188412923	31.73809020440298	24.56183986599537
40	24.78999595345807	17.25367034745733	32.9796037067472	24.976729992337397
41	23.689202620781625	19.72206092389344	27.823945770598378	28.764790684726556
42	27.859149728747763	16.442066051426476	31.368161655045213	24.330622564780544
43	26.99215659638949	15.712636212054104	34.19174868965702	23.10345850189939
44	19.35299334090346	17.58475974733507	33.07402301841415	29.988223893347314
45	20.062142639168037	14.801925120798366	33.10052164995595	32.03541059007765
46	25.410083064121906	15.630366092465875	34.09465081595696	24.86490002745526
47	18.190880070254856	16.47392180703448	40.29896578806643	25.036232334644232
48	20.01737238804328	14.959864617821813	35.453064418460265	29.569698575674643
49	23.828679172362595	12.479898826885492	39.37495754957492	24.31646445117699
50	21.634075900881726	12.188605205785311	37.627578474692854	28.549740418640113
51	18.585585318418847	12.73311860194368	31.28617852424625	37.39511755539123
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4655.0
1	4432.0
2	4209.0
3	4384.0
4	4559.0
5	4323.0
6	4087.0
7	4385.5
8	4684.0
9	5468.5
10	6253.0
11	6826.0
12	7399.0
13	7913.5
14	8428.0
15	8539.5
16	8651.0
17	8650.0
18	8649.0
19	8325.5
20	8002.0
21	7403.5
22	6805.0
23	6056.5
24	5308.0
25	4865.5
26	4916.5
27	5410.0
28	5925.0
29	6440.0
30	7088.5
31	7737.0
32	8081.5
33	8426.0
34	10165.0
35	11904.0
36	12709.0
37	13514.0
38	15899.0
39	18284.0
40	22459.0
41	26634.0
42	37607.5
43	48581.0
44	65967.0
45	83353.0
46	110858.0
47	138363.0
48	162535.0
49	186707.0
50	177056.0
51	167405.0
52	129207.0
53	91009.0
54	71040.5
55	51072.0
56	39580.0
57	28088.0
58	23233.5
59	18379.0
60	16186.0
61	13993.0
62	12650.0
63	11307.0
64	9784.5
65	8262.0
66	6671.5
67	5081.0
68	4114.0
69	3147.0
70	2879.5
71	2612.0
72	1987.0
73	1362.0
74	1225.5
75	802.0
76	515.0
77	362.5
78	210.0
79	211.0
80	212.0
81	159.5
82	107.0
83	62.0
84	17.0
85	10.0
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1045337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.23272319020433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.14119488091363	19.62719964613312
2	12.600985029629722	7.367222145901486
3	5.994006470039915	5.256633958169118
4	3.3854593375291913	3.9586478274273342
5	2.1840441605401004	3.1922779190125463
6	1.4817859686564052	2.5989983429316883
7	1.0625054723814764	2.1741949853543487
8	0.7767126686128764	1.8164341151908094
9	0.5875881458410042	1.5459121455493936
>10	4.2959602442610665	26.00327859912797
>50	0.34767653112952324	6.584508791069128
>100	0.11711593761857665	6.6934410793834695
>500	0.014685384027406958	2.90599682695113
>1k	0.009178365017129349	5.857928086887268
>5k	0.0	0.0
>10k+	0.0011014038020555218	4.417325530911163
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	15641	1.496263884278467	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	13106	1.2537583573527007	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	12412	1.1873682841035953	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	4644	0.44425864577643376	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	4584	0.4385188699912086	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	3888	0.37193747088259577	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3763	0.35997960466337653	No Hit
TGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCTT	3512	0.33596820929518423	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC	2764	0.2644123378393762	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	2514	0.2404966054009377	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	2362	0.22595584007836705	No Hit
GAAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	2353	0.2250948737105833	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTT	2262	0.2163895471029917	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	2212	0.21160640061530397	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	2165	0.20711024291687752	No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1989	0.19027356728021683	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1987	0.1900822414207093	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	1616	0.15459129448206654	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	1514	0.14483367564718364	No Hit
TGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1382	0.1322061689196881	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1305	0.12484012332864902	No Hit
GACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	1199	0.1146998527747511	No Hit
GTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1188	0.11364756054745981	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	1157	0.11068200972509344	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTC	1133	0.10838609941100336	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG	1070	0.10235933483651685	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.566292975375406E-5	0.0	0.0	1.5595927437754524	0.0
2	9.566292975375406E-5	0.0	0.0	4.96834991969097	0.0
3	9.566292975375406E-5	0.0	0.0	6.701475218039732	0.0
4	9.566292975375406E-5	0.0	0.0	8.744452745860904	0.0
5	9.566292975375406E-5	0.0	0.0	13.655500570629377	0.0
6	9.566292975375406E-5	0.0	0.0	16.745317538745876	0.0
7	9.566292975375406E-5	0.0	0.0	19.443586135380265	0.0
8	9.566292975375406E-5	0.0	0.0	23.80485910285391	0.0
9	9.566292975375406E-5	0.0	0.0	25.339962136612403	0.0
10	9.566292975375406E-5	0.0	0.0	28.450155308766455	0.0
11	9.566292975375406E-5	0.0	0.0	33.08291967088125	0.0
12	9.566292975375406E-5	0.0	0.0	36.93277861589134	0.0
13	9.566292975375406E-5	0.0	0.0	38.3307966713127	0.0
14	9.566292975375406E-5	0.0	0.0	38.83398368181744	0.0
15	9.566292975375406E-5	0.0	0.0	39.77922909071429	0.0
16	9.566292975375406E-5	0.0	0.0	41.66589339131782	0.0
17	9.566292975375406E-5	0.0	0.0	43.79841142138851	0.0
18	9.566292975375406E-5	0.0	0.0	46.79419172955707	0.0
19	9.566292975375406E-5	0.0	0.0	48.29916094044313	0.0
20	1.913258595075081E-4	0.0	0.0	49.58094853621368	0.0
21	2.8698878926126215E-4	0.0	0.0	50.78381421493739	0.0
22	2.8698878926126215E-4	0.0	0.0	51.76770744745475	0.0
23	2.8698878926126215E-4	0.0	0.0	52.698794742748035	0.0
24	2.8698878926126215E-4	0.0	0.0	53.44200004400495	0.0
25	2.8698878926126215E-4	0.0	0.0	54.07547996483431	0.0
26	2.8698878926126215E-4	0.0	0.0	54.652040442460184	0.0
27	2.8698878926126215E-4	0.0	0.0	55.16393277957252	0.0
28	2.8698878926126215E-4	0.0	0.0	55.67199859949471	0.0
29	2.8698878926126215E-4	0.0	0.0	56.236888199690625	0.0
30	2.8698878926126215E-4	0.0	0.0	56.79890791199393	0.0
31	4.783146487687703E-4	0.0	0.0	57.38063418782651	0.0
32	4.783146487687703E-4	0.0	0.0	57.92744349429897	0.0
33	4.783146487687703E-4	0.0	0.0	58.46114697939516	0.0
34	4.783146487687703E-4	0.0	0.0	59.003460128169195	0.0
35	5.739775785225243E-4	0.0	0.0	59.4955502388225	0.0
36	6.696405082762784E-4	0.0	0.0	60.01605223961268	0.0
37	6.696405082762784E-4	0.0	0.0	60.478008527393555	0.0
38	6.696405082762784E-4	0.0	0.0	60.96847236824105	0.0
39	6.696405082762784E-4	0.0	0.0	61.48715677336591	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	55	1.8189894E-12	45.000004	43
TATCACG	30	2.1653123E-6	45.000004	1
CGCATGT	55	1.8189894E-12	45.000004	38
CGCGCAT	55	1.8189894E-12	45.000004	36
CGCCCTA	30	2.1653123E-6	45.000004	17
TCAACGG	30	2.1653123E-6	45.000004	2
ACGGTGT	30	2.1653123E-6	45.000004	22
CTATGCG	20	7.0329936E-4	45.0	1
AAGTACG	40	6.8139343E-9	45.0	1
TTAGGCG	70	0.0	45.0	1
AGGTACG	40	6.8139343E-9	45.0	1
GGTACAT	20	7.0329936E-4	45.0	8
TCGCAAG	45	3.8562575E-10	45.0	1
AATCACG	25	3.8905637E-5	45.0	29
GATCGAC	20	7.0329936E-4	45.0	23
GGCACGT	20	7.0329936E-4	45.0	11
CGGCCAA	20	7.0329936E-4	45.0	7
TAGCGTG	25	3.8905637E-5	45.0	1
GCTATTA	40	6.8139343E-9	45.0	18
ATAGCCG	20	7.0329936E-4	45.0	1
>>END_MODULE