##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935058.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1010646
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.237329391300214	33.0	31.0	34.0	30.0	34.0
2	32.41423406415303	34.0	31.0	34.0	30.0	34.0
3	32.422565369080765	34.0	31.0	34.0	30.0	34.0
4	35.936769155569806	37.0	35.0	37.0	35.0	37.0
5	35.86574626526004	37.0	35.0	37.0	35.0	37.0
6	35.82335159887834	37.0	35.0	37.0	35.0	37.0
7	36.09665698968779	37.0	35.0	37.0	35.0	37.0
8	35.860079592656575	37.0	35.0	37.0	35.0	37.0
9	37.57412189827101	39.0	38.0	39.0	35.0	39.0
10	37.41701149561766	39.0	37.0	39.0	35.0	39.0
11	37.27070111591992	39.0	37.0	39.0	34.0	39.0
12	36.95760632308444	39.0	35.0	39.0	33.0	39.0
13	36.76893195045545	39.0	35.0	39.0	33.0	39.0
14	37.82850572801951	40.0	36.0	41.0	33.0	41.0
15	37.920611173447476	40.0	36.0	41.0	33.0	41.0
16	38.00463169101743	40.0	36.0	41.0	34.0	41.0
17	38.03291261232914	40.0	36.0	41.0	34.0	41.0
18	38.05424253398321	40.0	36.0	41.0	34.0	41.0
19	38.02439726669873	40.0	36.0	41.0	34.0	41.0
20	37.984422834503874	40.0	36.0	41.0	34.0	41.0
21	37.90346768304629	40.0	35.0	41.0	34.0	41.0
22	37.84687219857398	40.0	35.0	41.0	34.0	41.0
23	37.63591603786093	39.0	35.0	41.0	33.0	41.0
24	37.42327778470404	39.0	35.0	41.0	33.0	41.0
25	37.398560920440985	39.0	35.0	41.0	33.0	41.0
26	37.56839387876665	39.0	35.0	41.0	33.0	41.0
27	37.58972182148843	39.0	35.0	41.0	33.0	41.0
28	37.6006346435844	39.0	35.0	41.0	33.0	41.0
29	37.66424643247982	39.0	35.0	41.0	34.0	41.0
30	37.58308844046283	39.0	35.0	41.0	33.0	41.0
31	37.44793627046463	39.0	35.0	41.0	33.0	41.0
32	37.3566016191624	39.0	35.0	41.0	33.0	41.0
33	37.24453765215516	39.0	35.0	41.0	33.0	41.0
34	37.03879894641645	39.0	35.0	41.0	32.0	41.0
35	37.045564915905274	39.0	35.0	41.0	33.0	41.0
36	37.011643048109825	39.0	35.0	41.0	33.0	41.0
37	36.90671906879362	39.0	35.0	41.0	32.0	41.0
38	36.853750967203155	39.0	35.0	41.0	32.0	41.0
39	36.79516863471483	39.0	35.0	41.0	32.0	41.0
40	36.692602553218435	39.0	35.0	41.0	31.0	41.0
41	36.68995771021703	39.0	35.0	41.0	32.0	41.0
42	36.675578788220605	39.0	35.0	41.0	32.0	41.0
43	36.567507317102134	39.0	35.0	41.0	32.0	41.0
44	36.41837201156488	38.0	35.0	41.0	31.0	41.0
45	36.41553422266551	38.0	35.0	41.0	31.0	41.0
46	36.30510485372722	38.0	35.0	40.0	31.0	41.0
47	36.2612358827918	38.0	35.0	40.0	31.0	41.0
48	36.21640020343424	38.0	35.0	40.0	31.0	41.0
49	36.16586124122591	38.0	35.0	40.0	31.0	41.0
50	36.05559315526901	38.0	35.0	40.0	31.0	41.0
51	35.08830589543717	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	17.0
10	23.0
11	18.0
12	18.0
13	17.0
14	27.0
15	60.0
16	100.0
17	141.0
18	306.0
19	545.0
20	941.0
21	1394.0
22	2229.0
23	3190.0
24	4468.0
25	5473.0
26	6348.0
27	7378.0
28	8820.0
29	10600.0
30	13701.0
31	17913.0
32	23943.0
33	35526.0
34	76024.0
35	112573.0
36	70079.0
37	106651.0
38	198358.0
39	303539.0
40	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.05161055404167	23.570171949426406	25.637067776451893	10.741149720080028
2	30.490498156624575	25.394252784852462	25.434524056890346	18.68072500163262
3	29.997150337506902	25.29204093223542	27.085547263829273	17.6252614664284
4	27.8559456031093	25.424035715769914	27.316191821864432	19.403826859256355
5	23.80556594495006	30.215030782291723	25.44995972872796	20.529443544030254
6	26.215509683905147	33.38280664050518	24.464649343093427	15.937034332496244
7	79.41465161886556	6.617648513920798	8.563631578218288	5.404068288995355
8	79.47382169424309	4.755967965044141	8.631311062429377	7.138899278283395
9	73.97723832083638	7.466907304832751	10.966154321097594	7.589700053233278
10	45.04287356799512	23.882447464295115	16.795297265313472	14.279381702396291
11	33.19282914096529	24.21292915620306	22.841628028013766	19.75261367481789
12	29.544172737041457	21.51237921092054	28.50256172784536	20.440886324192643
13	23.95220482740742	27.803108111049763	28.98997275010241	19.254714311440406
14	18.60196349661504	30.47219303297099	29.850808294892573	21.0750351755214
15	17.64871181402786	24.58298949384849	37.59169877484302	20.17659991728063
16	20.094078440917986	25.921044559618306	32.60112838718997	21.383748612273735
17	19.75162420867445	24.376191069870163	27.028356120738618	28.84382860071677
18	21.74549743431429	25.9579516467685	30.674439912689504	21.6221110062277
19	22.709039564793212	27.121365938221693	26.624357094373302	23.545237402611797
20	25.326177514183996	27.79628079466005	25.956368500939003	20.921173190216948
21	21.913211945626855	28.842146508272926	28.998877945393343	20.245763600706876
22	21.64378031476897	25.079701497853847	26.50037698660065	26.776141200776532
23	20.135734965556683	27.126610108781907	26.793753698129713	25.943901227531697
24	22.555177579488763	23.618358950611785	29.77254152294671	24.05392194695274
25	18.79847147270162	26.892601365859065	26.813938807455823	27.49498835398349
26	18.49549693958122	28.26855298492251	27.291949901350225	25.94400017414604
27	19.871844345102044	27.71573825058428	28.470799864641034	23.941617539672645
28	16.886624990352704	26.00791968701207	31.859622459298308	25.245832863336915
29	18.388634596090025	23.68821526033844	29.556442117220076	28.36670802635146
30	20.093781601074955	25.615695307753654	30.262030424104978	24.028492667066413
31	23.77172620284452	26.67947035856274	25.87780488915011	23.670998549442636
32	24.07291969690673	25.48439315051957	23.787161874682134	26.655525277891567
33	24.886458760040608	24.626229164316683	27.026970868137806	23.460341207504904
34	19.639814534466073	24.53747405125039	28.657116339450212	27.165595074833327
35	18.624226484842367	25.436404042562877	26.17256685327998	29.766802619314774
36	24.064707127916204	24.877850404592706	28.081444937198587	22.975997530292506
37	21.15993137062829	26.493252830367904	30.00803446508471	22.338781333919098
38	20.036194671526925	27.686746892581578	25.72394290384566	26.55311553204584
39	23.06762209517477	24.521840486184086	28.275875034383947	24.134662384257197
40	22.29336483793534	23.703354092333022	28.450218968857545	25.553062100874097
41	19.9423932811291	24.181859919299143	25.519222358768552	30.356524440803206
42	22.585455243477934	21.97891249755107	27.446405566340736	27.989226692630258
43	23.460638047347935	20.876152480690568	28.666318374584176	26.996891097377322
44	19.982763499781328	22.643635852712027	29.272069547596292	28.101531099910353
45	20.183130393827316	23.82505842797577	26.36363276557766	29.628178412619256
46	22.088545346243887	23.64072088545346	29.27761055799954	24.993123210303114
47	19.018627689616345	23.633497782606373	30.80989782772603	26.537976700051253
48	20.064097616771846	21.05880793076903	31.71456672267045	27.16252772978867
49	22.377271566898795	19.546606823754313	33.409126439920605	24.666995169426286
50	19.992361321372666	21.47408687116953	31.037573987330873	27.495977820126928
51	19.104909137324046	22.11704197117487	27.82438163313366	30.953667258367418
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1383.0
1	1490.0
2	1597.0
3	1894.0
4	2191.0
5	1786.0
6	1381.0
7	1486.0
8	1591.0
9	1688.5
10	1786.0
11	1948.5
12	2111.0
13	2164.5
14	2218.0
15	2252.5
16	2287.0
17	2504.0
18	2721.0
19	2638.0
20	2555.0
21	2802.0
22	3049.0
23	3283.5
24	3518.0
25	3477.5
26	4331.5
27	5226.0
28	5930.0
29	6634.0
30	9243.5
31	11853.0
32	12701.0
33	13549.0
34	16177.5
35	18806.0
36	20791.0
37	22776.0
38	23512.5
39	24249.0
40	31469.5
41	38690.0
42	47746.5
43	56803.0
44	65770.0
45	74737.0
46	88617.5
47	102498.0
48	115003.5
49	127509.0
50	122268.0
51	117027.0
52	100283.0
53	83539.0
54	70697.0
55	57855.0
56	52400.0
57	46945.0
58	44930.5
59	42916.0
60	39416.0
61	35916.0
62	32328.0
63	28740.0
64	25226.0
65	21712.0
66	18768.0
67	15824.0
68	13388.0
69	10952.0
70	8233.0
71	5514.0
72	4621.5
73	3729.0
74	3003.0
75	1664.5
76	1052.0
77	1031.0
78	1010.0
79	598.5
80	187.0
81	208.0
82	229.0
83	135.0
84	41.0
85	27.5
86	14.0
87	12.0
88	10.0
89	5.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1010646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.301143937732892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.89997188950592	13.378448148214108
2	12.62915363718336	5.127725316004046
3	6.088443097268503	3.7080707902303245
4	3.467237434793518	2.815555449201559
5	2.1802648362787207	2.213093513183597
6	1.4741829555692845	1.795656022293872
7	1.0865432028055924	1.5440648968305142
8	0.7746602523594583	1.2581191428791878
9	0.5970274353794726	1.0908305910372773
>10	3.5747526786239483	14.624737474033042
>50	1.0951523126812706	16.471216028234352
>100	1.118809968381481	32.0323062428682
>500	0.008378753067174087	1.101336282525818
>1k	0.004435810447327457	1.589618381074559
>5k	9.857356549616571E-4	1.2492217213895556
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	6889	0.6816432262137286	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	5596	0.5537052538673285	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	4246	0.4201273245033375	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	2433	0.2407371126982148	No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	2148	0.21253732761026115	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	1313	0.12991690463327416	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	1258	0.12447484084437083	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	1176	0.11636121846818769	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1143	0.11309598019484568	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC	1096	0.10844548932069191	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	1074	0.10626866380513057	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.8946614343697E-5	0.0	0.0	0.44308293903107515	0.0
2	9.8946614343697E-5	0.0	0.0	2.2233304243028718	0.0
3	9.8946614343697E-5	0.0	0.0	3.097721655258122	0.0
4	9.8946614343697E-5	0.0	0.0	4.0546343625760155	0.0
5	9.8946614343697E-5	0.0	0.0	6.627444228740825	0.0
6	9.8946614343697E-5	0.0	0.0	7.884659910591839	0.0
7	9.8946614343697E-5	0.0	0.0	9.191249953000359	0.0
8	9.8946614343697E-5	0.0	0.0	11.055107327392578	0.0
9	9.8946614343697E-5	0.0	0.0	11.69667717479711	0.0
10	9.8946614343697E-5	0.0	0.0	13.235791760913317	0.0
11	9.8946614343697E-5	0.0	0.0	15.678981562287882	0.0
12	9.8946614343697E-5	0.0	0.0	17.421629334109074	0.0
13	9.8946614343697E-5	0.0	0.0	18.101491521264617	0.0
14	9.8946614343697E-5	0.0	0.0	18.353310654769327	0.0
15	9.8946614343697E-5	0.0	0.0	18.857542601464807	0.0
16	9.8946614343697E-5	0.0	0.0	19.912511403597303	0.0
17	9.8946614343697E-5	0.0	0.0	21.078894093480802	0.0
18	9.8946614343697E-4	0.0	0.0	22.49323699890961	0.0
19	9.8946614343697E-4	0.0	0.0	23.312712858904106	0.0
20	9.8946614343697E-4	0.0	0.0	24.061342943028517	0.0
21	9.8946614343697E-4	0.0	0.0	24.935734173983768	0.0
22	9.8946614343697E-4	0.0	0.0	25.827540009063508	0.0
23	9.8946614343697E-4	0.0	0.0	26.716971125399002	0.0
24	9.8946614343697E-4	0.0	0.0	27.403363789101228	0.0
25	9.8946614343697E-4	0.0	0.0	27.97586889969386	0.0
26	0.001088412757780667	0.0	0.0	28.49613019791302	0.0
27	0.001088412757780667	0.0	0.0	29.004023169339213	0.0
28	0.001088412757780667	0.0	0.0	29.543875897198426	0.0
29	0.001088412757780667	0.0	0.0	30.088774902389165	0.0
30	0.001088412757780667	0.0	0.0	30.706795455579897	0.0
31	0.001088412757780667	0.0	0.0	31.294439398167114	0.0
32	0.001088412757780667	0.0	0.0	31.807477593539183	0.0
33	0.001088412757780667	0.0	0.0	32.3111158605486	0.0
34	0.001088412757780667	0.0	0.0	32.84562547123325	0.0
35	0.0011873593721243641	0.0	0.0	33.418625314897604	0.0
36	0.0011873593721243641	0.0	0.0	33.9201857030058	0.0
37	0.0011873593721243641	0.0	0.0	34.439259641852836	0.0
38	0.001286305986468061	0.0	0.0	34.92864959639676	0.0
39	0.001286305986468061	0.0	0.0	35.44406251051308	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	30	2.1652413E-6	45.000004	18
AACGTAG	30	2.1652413E-6	45.000004	1
AATCCCG	35	1.211738E-7	45.000004	23
CGAGTTA	35	1.211738E-7	45.000004	6
CAACGTT	30	2.1652413E-6	45.000004	15
TAGCGCG	45	3.8380676E-10	45.000004	1
CTCCCGT	35	1.211738E-7	45.000004	21
GTTAGTG	45	3.8380676E-10	45.000004	1
TCGATTG	30	2.1652413E-6	45.000004	1
CGTTCGG	45	3.8380676E-10	45.000004	2
TCGGCCC	35	1.211738E-7	45.000004	14
GTTACGT	35	1.211738E-7	45.000004	35
TACGATA	70	0.0	45.000004	17
TTGCGCA	35	1.211738E-7	45.000004	15
GCGATAT	30	2.1652413E-6	45.000004	9
ACGTCCA	35	1.211738E-7	45.000004	36
ATGCGAG	30	2.1652413E-6	45.000004	1
TACTACG	45	3.8380676E-10	45.000004	1
GCGTGAA	35	1.211738E-7	45.000004	39
GTAAGTC	35	1.211738E-7	45.000004	19
>>END_MODULE