##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935057.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	966392
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15765755511221	33.0	31.0	34.0	30.0	34.0
2	32.32520861099844	34.0	31.0	34.0	30.0	34.0
3	32.34846521908294	34.0	31.0	34.0	30.0	34.0
4	35.89970943468075	37.0	35.0	37.0	35.0	37.0
5	35.81564313446303	37.0	35.0	37.0	35.0	37.0
6	35.74356782754824	37.0	35.0	37.0	35.0	37.0
7	36.055780676992356	37.0	35.0	37.0	35.0	37.0
8	35.842541122029154	37.0	35.0	37.0	35.0	37.0
9	37.61925388455202	39.0	38.0	39.0	35.0	39.0
10	37.35389262328331	39.0	37.0	39.0	34.0	39.0
11	37.26889709351899	39.0	37.0	39.0	34.0	39.0
12	37.07872685204348	39.0	35.0	39.0	34.0	39.0
13	36.88547814965356	39.0	35.0	39.0	33.0	39.0
14	37.940347188304536	40.0	37.0	41.0	33.0	41.0
15	38.042865628026725	40.0	36.0	41.0	33.0	41.0
16	38.130063162774526	40.0	36.0	41.0	34.0	41.0
17	38.181299100158114	40.0	36.0	41.0	34.0	41.0
18	38.19674831745296	40.0	36.0	41.0	34.0	41.0
19	38.15011817150804	40.0	36.0	41.0	34.0	41.0
20	38.11062695055423	40.0	36.0	41.0	34.0	41.0
21	38.02709666470749	40.0	36.0	41.0	34.0	41.0
22	37.95445430011838	40.0	36.0	41.0	34.0	41.0
23	37.74067148734675	39.0	35.0	41.0	33.0	41.0
24	37.531309240970536	39.0	35.0	41.0	33.0	41.0
25	37.49206533166665	39.0	35.0	41.0	33.0	41.0
26	37.640327113635045	39.0	35.0	41.0	33.0	41.0
27	37.70012375930264	39.0	35.0	41.0	33.0	41.0
28	37.70496444506991	39.0	36.0	41.0	33.0	41.0
29	37.738541916737724	40.0	36.0	41.0	34.0	41.0
30	37.60497499979304	39.0	36.0	41.0	33.0	41.0
31	37.51781885611636	39.0	35.0	41.0	33.0	41.0
32	37.40982334290847	39.0	35.0	41.0	33.0	41.0
33	37.33930744459805	39.0	35.0	41.0	33.0	41.0
34	37.27736881100009	39.0	35.0	41.0	33.0	41.0
35	37.18521986936978	39.0	35.0	41.0	33.0	41.0
36	37.15026304025695	39.0	35.0	41.0	33.0	41.0
37	37.12397660576661	39.0	35.0	41.0	33.0	41.0
38	37.02248569938493	39.0	35.0	41.0	32.0	41.0
39	36.98862883798707	39.0	35.0	41.0	32.0	41.0
40	36.91586540451494	39.0	35.0	41.0	32.0	41.0
41	36.88483658805123	39.0	35.0	41.0	32.0	41.0
42	36.92420053146135	39.0	35.0	41.0	32.0	41.0
43	36.8685698971018	39.0	35.0	41.0	32.0	41.0
44	36.70671321782465	39.0	35.0	41.0	31.0	41.0
45	36.69751198271509	39.0	35.0	41.0	31.0	41.0
46	36.543330242800025	39.0	35.0	41.0	31.0	41.0
47	36.49951365491436	39.0	35.0	40.0	31.0	41.0
48	36.493054578266374	39.0	35.0	40.0	31.0	41.0
49	36.47953521966242	39.0	35.0	40.0	31.0	41.0
50	36.39298855950794	39.0	35.0	40.0	31.0	41.0
51	35.34484867424399	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	10.0
11	13.0
12	12.0
13	13.0
14	18.0
15	31.0
16	58.0
17	98.0
18	221.0
19	404.0
20	771.0
21	1233.0
22	1875.0
23	2838.0
24	3958.0
25	5000.0
26	5670.0
27	6851.0
28	7985.0
29	9746.0
30	12583.0
31	16529.0
32	22187.0
33	32335.0
34	63813.0
35	98550.0
36	67858.0
37	107418.0
38	209342.0
39	288788.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.79834270151243	22.43975529598755	26.714728598746678	11.047173403753343
2	29.318640882788767	25.290772274604922	27.167546916779113	18.2230399258272
3	29.804054669326735	24.132132716330435	28.467743938277636	17.596068676065197
4	27.936489540476327	26.96617935578937	26.9388612488514	18.158469854882906
5	24.34395152277751	29.17760080795371	26.339208106027368	20.139239563241418
6	24.599748342287604	33.95216433910877	25.573783723375193	15.874303595228437
7	76.80889328554045	6.569487330193131	10.13719070522107	6.484428679045356
8	77.3836083080158	4.579922019222014	9.62114752605568	8.415322146706512
9	73.21035356252949	6.648544276028775	12.240374506411476	7.9007276550302565
10	39.51264083311948	23.02492156392023	20.28886828533349	17.173569317626804
11	30.776330929891806	25.85306997574483	23.921348686661315	19.449250407702053
12	28.78986994925455	21.805023220390897	29.77249397759915	19.632612852755404
13	21.93364597389051	28.2928666628035	31.21693888194439	18.556548481361602
14	17.14221558125481	32.34805337792531	29.401423025025043	21.108308015794833
15	15.141474681081796	26.69982781314415	38.7562190084355	19.402478497338553
16	15.76792854245482	26.05143668407851	35.166785321070535	23.01384945239613
17	16.521763425193917	25.882354158560915	28.273205904022387	29.322676512222785
18	18.29733689848426	26.26128941464747	32.76610319621851	22.67527049064976
19	20.58367618937243	28.289037988725074	28.407519929800745	22.719765892101755
20	23.335354597306267	28.194769824253513	28.41828160829146	20.051593970148758
21	20.40672936034239	28.96454026937309	29.74879758938402	20.879932780900504
22	17.93247460657787	27.666516279108272	26.761086598398993	27.639922515914865
23	17.43309133353753	28.515757580774675	28.56304687952715	25.488104206160646
24	20.943468075066846	23.94990852573283	31.37732928252717	23.729294116673152
25	16.851753739683275	27.820387585989952	28.700465235639367	26.627393438687406
26	17.521461270374754	31.527992781397195	28.825259315060553	22.125286633167494
27	21.713238520186426	29.08809261666073	27.351633705577033	21.84703515757581
28	16.73130572272949	27.356704111789004	30.18236905934652	25.729621106134985
29	20.60323347047575	27.208420599508276	28.504685469250575	23.683660460765406
30	23.04675535393505	29.474271310192968	24.934084719244364	22.54488861662762
31	21.373521303984305	30.0538497835247	23.874990687009	24.697638225482
32	24.472677753954915	31.670895454432568	22.086585981672034	21.76984080994048
33	23.110911514168166	29.280664575037875	22.90871613175606	24.699707779037904
34	18.638916712886697	27.21628490302072	27.612707886654693	26.532090497437892
35	20.23371468306857	28.80083858310085	25.2890131540824	25.676433579748174
36	22.69917383422048	27.74091672944313	25.068605700378317	24.491303735958077
37	20.829021763425192	26.49545939949834	29.201090240813254	23.474428596263213
38	20.429494449457366	30.18764642091408	23.89899750825752	25.48386162137104
39	21.480620700502488	25.700026490285516	28.773830909196267	24.04552190001573
40	22.123527512644973	26.183474200945373	30.014735221318055	21.6782630650916
41	17.782535451452414	28.22881398024818	26.239352146954857	27.749298421344548
42	20.99893211036515	25.02421377660411	26.606801380806132	27.370052732224604
43	24.311045621238588	23.509921439747018	26.250217303123367	25.928815635891027
44	20.965094909726073	24.728371095787217	26.70231127741124	27.604222717075473
45	21.383041250341474	24.769141300838584	26.104831165820908	27.742986282999034
46	22.68127219596189	26.339415061382958	27.910309687994108	23.06900305466105
47	18.034814029917467	25.0199711918145	31.37319017541536	25.57202460285267
48	19.412308876729114	22.977632265167756	29.768768781198517	27.841290076904613
49	21.26756016192187	19.6437884419573	32.99240887755693	26.096242518563894
50	20.2041200672191	21.276976630601247	31.05996324472885	27.45894005745081
51	18.25284149703226	22.480835934072303	26.864874709227728	32.40144785966771
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1676.0
1	1615.5
2	1555.0
3	1817.5
4	2080.0
5	1627.5
6	1175.0
7	1321.5
8	1468.0
9	1606.0
10	1744.0
11	1861.0
12	1978.0
13	2139.5
14	2301.0
15	2351.0
16	2401.0
17	2377.0
18	2353.0
19	2376.5
20	2400.0
21	2376.5
22	2353.0
23	2847.5
24	3342.0
25	3521.5
26	4422.5
27	5144.0
28	6706.5
29	8269.0
30	9710.0
31	11151.0
32	13181.0
33	15211.0
34	17291.5
35	19372.0
36	21429.5
37	23487.0
38	25813.5
39	28140.0
40	33870.0
41	39600.0
42	51108.5
43	62617.0
44	71202.5
45	79788.0
46	94922.0
47	110056.0
48	126922.5
49	143789.0
50	137750.5
51	131712.0
52	105578.0
53	79444.0
54	66826.0
55	54208.0
56	45413.5
57	36619.0
58	31470.0
59	26321.0
60	24084.0
61	21847.0
62	18989.0
63	16131.0
64	13358.5
65	10586.0
66	7949.0
67	5312.0
68	3941.0
69	2570.0
70	2324.5
71	2079.0
72	1662.0
73	1245.0
74	857.0
75	396.0
76	323.0
77	229.5
78	136.0
79	126.0
80	116.0
81	96.5
82	77.0
83	46.5
84	16.0
85	18.5
86	21.0
87	12.5
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	966392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.295010522038844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.416068208743845	13.291508292494944
2	12.964342772255574	5.521516314930013
3	6.520022426378705	4.165318385209912
4	3.9476530997501555	3.3626125718615527
5	2.613096017808444	2.782295359716431
6	1.8261938226994658	2.333329000180047
7	1.3623223250198313	2.030746777199401
8	1.0455927902656355	1.781272757637973
9	0.7890048510765882	1.5121679945054074
>10	4.674454509414887	19.45849101253708
>50	1.1478999527417233	17.76348602510054
>100	0.6756967683583004	20.46147006945794
>500	0.011277957672163214	1.6433498300789926
>1k	0.004903459857462267	1.7798257627205765
>5k	0.00147103795723868	2.112609846369182
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	7856	0.8129206367602381	TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	7236	0.7487644765271236	TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	5140	0.5318752638680784	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	3600	0.37251964006324556	TruSeq Adapter, Index 23 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2580	0.2669724087119926	TruSeq Adapter, Index 20 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	1575	0.16297734252766993	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	1535	0.1588382354158561	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	1454	0.15045654351443308	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1347	0.13938443199033104	No Hit
AGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	1334	0.13803922217899153	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCC	1275	0.13193403918906615	No Hit
AAAACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	1188	0.12293148122087103	No Hit
GGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	1157	0.11972367320921531	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	989	0.10233942333959718	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5549507860164405	0.0
2	0.0	0.0	0.0	2.8724368579210093	0.0
3	0.0	0.0	0.0	4.030352072450931	0.0
4	0.0	0.0	0.0	5.389013981903823	0.0
5	0.0	0.0	0.0	8.597960248015298	0.0
6	0.0	0.0	0.0	10.25836306591942	0.0
7	0.0	0.0	0.0	12.045836472156227	0.0
8	0.0	0.0	0.0	14.683275523804005	0.0
9	0.0	0.0	0.0	15.536242021871042	0.0
10	0.0	0.0	0.0	17.66612306393265	0.0
11	0.0	0.0	0.0	20.794770652074934	0.0
12	0.0	0.0	0.0	23.015815528274242	0.0
13	0.0	0.0	0.0	23.925798226806513	0.0
14	0.0	0.0	0.0	24.241198188726727	0.0
15	0.0	0.0	0.0	24.811153238023493	0.0
16	0.0	0.0	0.0	26.27443107972748	0.0
17	0.0	0.0	0.0	27.875851621288255	0.0
18	0.0	0.0	0.0	29.867589963493074	0.0
19	0.0	0.0	0.0	30.935686553696637	0.0
20	0.0	0.0	0.0	31.857051796786397	0.0
21	0.0014486874891348439	0.0	0.0	33.11751339001151	0.0
22	0.0014486874891348439	0.0	0.0	34.33296219339564	0.0
23	0.0014486874891348439	0.0	0.0	35.568589143949865	0.0
24	0.0014486874891348439	0.0	0.0	36.468637985413785	0.0
25	0.0014486874891348439	0.0	0.0	37.25217096168014	0.0
26	0.0014486874891348439	0.0	0.0	37.95861306798897	0.0
27	0.0014486874891348439	0.0	0.0	38.59769120605303	0.0
28	0.0014486874891348439	0.0	0.0	39.2676056921001	0.0
29	0.0014486874891348439	0.0	0.0	39.974461709120106	0.0
30	0.0014486874891348439	0.0	0.0	40.77548241293388	0.0
31	0.0014486874891348439	0.0	0.0	41.57971092475931	0.0
32	0.0014486874891348439	0.0	0.0	42.27704699542215	0.0
33	0.0014486874891348439	0.0	0.0	42.93754501278984	0.0
34	0.0014486874891348439	0.0	0.0	43.55303024031656	0.0
35	0.0014486874891348439	0.0	0.0	44.2437437396005	0.0
36	0.0014486874891348439	0.0	0.0	44.90672522123528	0.0
37	0.0014486874891348439	0.0	0.0	45.535559069197596	0.0
38	0.0014486874891348439	0.0	0.0	46.11958708267453	0.0
39	0.0014486874891348439	0.0	0.0	46.7136524308976	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	35	1.2116652E-7	45.000004	24
AGGTACG	30	2.1651413E-6	45.000004	1
TCGTTGC	30	2.1651413E-6	45.000004	39
CAACGTT	35	1.2116652E-7	45.000004	21
TGGAACG	30	2.1651413E-6	45.000004	1
GCTATCA	30	2.1651413E-6	45.000004	30
ACCGAGC	35	1.2116652E-7	45.000004	2
TTGTACG	30	2.1651413E-6	45.000004	1
TTCCGGT	30	2.1651413E-6	45.000004	12
TGACGTA	30	2.1651413E-6	45.000004	16
CGTTCCG	30	2.1651413E-6	45.000004	40
CGTTAGT	30	2.1651413E-6	45.000004	14
AGACGCG	30	2.1651413E-6	45.000004	2
TAACCGA	35	1.2116652E-7	45.000004	28
TACGAGA	35	1.2116652E-7	45.000004	35
ACTACGT	30	2.1651413E-6	45.000004	10
ACTACCC	35	1.2116652E-7	45.000004	12
TTGCGAA	45	3.8380676E-10	45.000004	26
GTCCGAC	30	2.1651413E-6	45.000004	19
CGTAATC	30	2.1651413E-6	45.000004	19
>>END_MODULE