SRR2935056.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935056.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1477338
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	5814	0.39354568825820496	Illumina Paired End PCR Primer 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	5806	0.39300417372327795	Illumina Paired End PCR Primer 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	4257	0.28815342189803556	TruSeq Adapter, Index 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2256	0.152707098849417	Illumina Paired End PCR Primer 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1751	0.11852399383214945	TruSeq Adapter, Index 1 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1665	0.11270271258168407	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCATA	20	7.03394E-4	45.000004	36
GATCGTC	30	2.1659234E-6	45.000004	10
GGCCGTA	30	2.1659234E-6	45.000004	27
ATTACGT	30	2.1659234E-6	45.000004	11
ATTACGA	20	7.03394E-4	45.000004	25
GAATCGT	20	7.03394E-4	45.000004	12
ATTCGCG	20	7.03394E-4	45.000004	5
TAAGTCG	20	7.03394E-4	45.000004	2
CCTCGAC	30	2.1659234E-6	45.000004	29
CTAACGC	20	7.03394E-4	45.000004	44
CCCGTCA	20	7.03394E-4	45.000004	18
TCGGAAA	20	7.03394E-4	45.000004	36
TATCTCG	20	7.03394E-4	45.000004	44
TACGATA	20	7.03394E-4	45.000004	17
ACGGAGT	20	7.03394E-4	45.000004	27
TGCTCGA	20	7.03394E-4	45.000004	39
CTCGACT	20	7.03394E-4	45.000004	21
GTGCGTA	20	7.03394E-4	45.000004	21
AATCGAA	20	7.03394E-4	45.000004	15
TCGTTGC	25	3.8913488E-5	45.0	34