##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935056.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1477338
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.318676565552366	33.0	31.0	34.0	30.0	34.0
2	32.49078477640188	34.0	31.0	34.0	31.0	34.0
3	32.49060539971219	34.0	31.0	34.0	31.0	34.0
4	36.01036052683949	37.0	35.0	37.0	35.0	37.0
5	35.925849060946106	37.0	35.0	37.0	35.0	37.0
6	35.86870303207526	37.0	35.0	37.0	35.0	37.0
7	36.13464691221643	37.0	35.0	37.0	35.0	37.0
8	35.93497764221864	37.0	35.0	37.0	35.0	37.0
9	37.67798499733981	39.0	38.0	39.0	35.0	39.0
10	37.479526012327575	39.0	37.0	39.0	35.0	39.0
11	37.367726952126056	39.0	37.0	39.0	35.0	39.0
12	37.10778440681821	39.0	37.0	39.0	34.0	39.0
13	36.93052639274154	39.0	35.0	39.0	33.0	39.0
14	37.81727336601374	40.0	37.0	41.0	33.0	41.0
15	37.9432222010129	40.0	37.0	41.0	33.0	41.0
16	38.11340126633174	40.0	37.0	41.0	33.0	41.0
17	38.19417357436145	40.0	37.0	41.0	34.0	41.0
18	38.209254077266	40.0	36.0	41.0	34.0	41.0
19	38.17979027142062	40.0	36.0	41.0	34.0	41.0
20	38.1163437209359	40.0	36.0	41.0	34.0	41.0
21	38.0334134774845	40.0	36.0	41.0	34.0	41.0
22	38.03854974284828	40.0	36.0	41.0	34.0	41.0
23	37.85650338649652	40.0	35.0	41.0	34.0	41.0
24	37.631177834727055	39.0	35.0	41.0	33.0	41.0
25	37.598216521879216	39.0	35.0	41.0	33.0	41.0
26	37.75721398894498	40.0	35.0	41.0	34.0	41.0
27	37.80451189910501	40.0	35.0	41.0	34.0	41.0
28	37.77197161380808	40.0	35.0	41.0	34.0	41.0
29	37.800242056997114	40.0	36.0	41.0	34.0	41.0
30	37.70186917279594	40.0	35.0	41.0	33.0	41.0
31	37.564844334878	40.0	35.0	41.0	33.0	41.0
32	37.52261432387172	40.0	35.0	41.0	33.0	41.0
33	37.45167185843727	40.0	35.0	41.0	33.0	41.0
34	37.22882508945143	39.0	35.0	41.0	32.0	41.0
35	37.18555198607225	39.0	35.0	41.0	32.0	41.0
36	37.14144021205709	39.0	35.0	41.0	32.0	41.0
37	37.0638682549288	39.0	35.0	41.0	32.0	41.0
38	36.993683232950076	39.0	35.0	41.0	32.0	41.0
39	36.91331503014205	39.0	35.0	41.0	32.0	41.0
40	36.78794967705427	39.0	35.0	41.0	32.0	41.0
41	36.696014046887036	39.0	35.0	41.0	31.0	41.0
42	36.75609440764402	39.0	35.0	41.0	32.0	41.0
43	36.7188131625938	39.0	35.0	41.0	32.0	41.0
44	36.601621971410744	39.0	35.0	41.0	31.0	41.0
45	36.56398806501965	39.0	35.0	41.0	32.0	41.0
46	36.44528334071147	38.0	35.0	40.0	31.0	41.0
47	36.377941946934286	38.0	35.0	40.0	31.0	41.0
48	36.32563299664667	38.0	35.0	40.0	31.0	41.0
49	36.25964606609997	38.0	35.0	40.0	31.0	41.0
50	36.144092956385066	38.0	35.0	40.0	31.0	41.0
51	35.11951225785839	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	14.0
10	26.0
11	19.0
12	19.0
13	28.0
14	45.0
15	63.0
16	105.0
17	216.0
18	332.0
19	652.0
20	1096.0
21	1721.0
22	2610.0
23	3804.0
24	5530.0
25	7099.0
26	8677.0
27	10413.0
28	12411.0
29	15258.0
30	19392.0
31	25520.0
32	34896.0
33	54062.0
34	109300.0
35	131539.0
36	107642.0
37	171582.0
38	300989.0
39	451979.0
40	291.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.46566324023345	21.902232258291605	25.15240249692352	11.47970200455143
2	31.41677801559291	24.852741891158285	25.498836420643073	18.231643672605728
3	31.5604147459823	24.305947589515736	26.626201993044248	17.507435671457717
4	28.66344736275653	25.751385261869657	26.06262074081896	19.522546634554853
5	25.856100635061168	28.96480020144341	24.729141198561198	20.449957964934224
6	27.332878461123993	33.32162308151554	23.677113835831744	15.668384621528721
7	81.18764967800192	6.2052150557286145	7.7743887993133605	4.832746466956106
8	81.83123970276267	3.985546977062798	8.284157044630275	5.899056275544256
9	76.44256087638712	6.459929955094908	10.54125731552292	6.556251852995048
10	45.21680211298972	22.919264244201397	16.989070882898837	14.874862759910053
11	33.4706749572542	23.464027866337965	22.386481631150083	20.678815545257752
12	30.095211793103545	20.454290081213642	28.93318929046704	20.517308835215772
13	26.688814611145183	23.859536544785282	29.94656605326608	19.505082790803456
14	20.49815275854273	26.755150141673738	32.0057427616429	20.74095433814063
15	17.78685717148005	24.449719698538857	37.946834102960864	19.81658902702022
16	23.328107718071287	24.404164788288124	31.909488553059624	20.358238940580964
17	24.276231979411616	23.309290087982575	26.932496151862335	25.481981780743475
18	24.86255684210384	23.73045301752206	30.124182820722133	21.28280731965197
19	25.991817715377252	25.384238407189148	26.959301121341223	21.664642756092377
20	25.85968816885506	27.592737748572095	26.862505398223018	19.685068684349822
21	23.062088702788394	26.08448439016664	30.84277260857028	20.01065429847469
22	22.280480160938122	23.917884735923668	28.930684785743004	24.870950317395206
23	22.117551975241955	25.500460964247857	29.12759300850584	23.254394052004347
24	22.857260829952253	24.7374669845357	28.008620911396036	24.39665127411601
25	23.04970155780194	25.505266905745334	26.652939273206265	24.79209226324646
26	20.967172035106387	26.835023535575473	27.2926033175888	24.90520111172934
27	22.652703714383573	26.134912931231714	28.429648462301788	22.782734892082924
28	19.20528680640449	25.516164885760737	28.983888588799584	26.294659719035185
29	22.584540572299634	24.99448332067543	28.24167522936525	24.17930087765968
30	25.784011512599015	24.072757892912794	26.51404079499749	23.629189799490703
31	24.017591099667104	25.757274232436995	25.37036209723164	24.854772570664263
32	26.198811646353104	25.22956831815062	24.570274371876984	24.001345663619293
33	27.46967857050993	24.33248180172716	25.253191889736808	22.9446477380261
34	21.645757436686797	25.04782250236574	29.327614939844505	23.978805121102955
35	21.858369580962513	25.031509377001065	28.82420949031298	24.285911551723437
36	26.205445199405958	24.4690788431625	27.533103460413255	21.792372497018285
37	23.27476853638098	26.86074547598451	26.91977056029155	22.944715427342967
38	22.8103521333642	25.656281771673108	25.825640442471524	25.70772565249117
39	23.81046179005752	23.016127656636463	27.71701533433784	25.45639521896817
40	25.41510473567999	22.791534503275486	29.647311583402036	22.14604917764249
41	20.731004008561346	23.456920488067052	29.67865173711094	26.133423766260666
42	22.073080094061076	21.990363748850974	31.549042940748834	24.38751321633912
43	23.309899291834366	21.573126799689714	30.131425577626786	24.985548330849134
44	22.989661133741908	21.882466977766768	28.08727589759419	27.040595990897142
45	22.768926271442282	22.21549841674688	26.55803885096031	28.45753646085053
46	23.27882989539293	23.330612222795324	28.561033426338454	24.82952445547329
47	19.482677626920854	24.661790328279647	31.537874203465964	24.317657841333535
48	21.447766184854107	23.2185864033823	29.97716162448945	25.356485787274135
49	23.861161088390066	21.17572282037015	30.594826640890577	24.36828945034921
50	20.634952867928664	23.02357348149171	29.432601070303477	26.908872580276146
51	20.18888026978254	23.933520968119684	26.384754199783668	29.492844562314108
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	761.0
1	1146.0
2	1531.0
3	2265.5
4	3000.0
5	2454.0
6	1908.0
7	1891.0
8	1874.0
9	2053.0
10	2232.0
11	2333.0
12	2434.0
13	2441.0
14	2448.0
15	2616.0
16	2784.0
17	2837.0
18	2890.0
19	3347.0
20	3804.0
21	3997.5
22	4191.0
23	4506.0
24	4821.0
25	5583.0
26	8081.0
27	9817.0
28	11187.5
29	12558.0
30	14799.5
31	17041.0
32	20212.0
33	23383.0
34	25078.0
35	26773.0
36	30747.0
37	34721.0
38	38260.0
39	41799.0
40	48872.5
41	55946.0
42	64585.0
43	73224.0
44	82346.5
45	91469.0
46	109545.5
47	127622.0
48	144156.5
49	160691.0
50	162858.0
51	165025.0
52	143445.0
53	121865.0
54	108820.0
55	95775.0
56	88865.5
57	81956.0
58	75666.5
59	69377.0
60	66438.5
61	63500.0
62	56459.5
63	49419.0
64	43816.5
65	38214.0
66	31879.0
67	25544.0
68	21312.5
69	17081.0
70	15274.0
71	13467.0
72	10996.0
73	8525.0
74	7051.5
75	4326.0
76	3074.0
77	2499.5
78	1925.0
79	1271.5
80	618.0
81	386.0
82	154.0
83	143.5
84	133.0
85	78.0
86	23.0
87	16.0
88	9.0
89	6.0
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1477338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.886575897213948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.92623924679584	13.769733996460864
2	12.745357506348745	5.324137537869572
3	5.86522926150349	3.6751366837485833
4	3.229678776082971	2.698277235211121
5	2.0759230564329387	2.1679462237481464
6	1.4267428718568824	1.7879863967308662
7	0.9996182200915465	1.4615021275526003
8	0.7175576929687274	1.1989858571856855
9	0.6027047915226524	1.1329595434577149
>10	4.123730874808196	17.81420332359689
>50	1.4020237067598722	21.683452390541504
>100	0.8737235061876667	24.28785798489034
>500	0.0072100214311740735	0.9552366218662117
>1k	0.0036050107155870367	1.247181028334101
>5k	6.554564937430975E-4	0.7954030488058317
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	5814	0.39354568825820496	Illumina Paired End PCR Primer 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	5806	0.39300417372327795	Illumina Paired End PCR Primer 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	4257	0.28815342189803556	TruSeq Adapter, Index 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2256	0.152707098849417	Illumina Paired End PCR Primer 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1751	0.11852399383214945	TruSeq Adapter, Index 1 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1665	0.11270271258168407	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3010820814194179	0.0
2	0.0	0.0	0.0	1.412066839139046	0.0
3	0.0	0.0	0.0	2.015043273780272	0.0
4	0.0	0.0	0.0	2.7381005565415633	0.0
5	0.0	0.0	0.0	4.621555798334572	0.0
6	6.768931686587632E-5	0.0	0.0	5.594928174865873	0.0
7	6.768931686587632E-5	0.0	0.0	6.661102604820291	0.0
8	6.768931686587632E-5	0.0	0.0	8.19819161221061	0.0
9	6.768931686587632E-5	0.0	0.0	8.733614108619692	0.0
10	6.768931686587632E-5	0.0	0.0	9.962852102904007	0.0
11	6.768931686587632E-5	0.0	0.0	12.038680383229837	0.0
12	6.768931686587632E-5	0.0	0.0	13.57177572092507	0.0
13	6.768931686587632E-5	0.0	0.0	14.154513049823398	0.0
14	6.768931686587632E-5	0.0	0.0	14.380392300204829	0.0
15	6.768931686587632E-5	0.0	0.0	14.831880043700224	0.0
16	6.768931686587632E-5	0.0	0.0	15.71718861898902	0.0
17	6.768931686587632E-5	0.0	0.0	16.749450701193634	0.0
18	6.768931686587632E-5	0.0	0.0	17.982343918588704	0.0
19	6.768931686587632E-5	0.0	0.0	18.68990034778771	0.0
20	6.768931686587632E-5	0.0	0.0	19.412686873281537	0.0
21	6.768931686587632E-5	0.0	0.0	20.345107213108985	0.0
22	6.768931686587632E-5	0.0	0.0	21.248082700099776	0.0
23	6.768931686587632E-5	0.0	0.0	22.086144132216187	0.0
24	1.3537863373175264E-4	0.0	0.0	22.738669146803236	0.0
25	1.3537863373175264E-4	0.0	0.0	23.29785059343224	0.0
26	1.3537863373175264E-4	0.0	0.0	23.86170260292499	0.0
27	1.3537863373175264E-4	0.0	0.0	24.512332316639792	0.0
28	1.3537863373175264E-4	0.0	0.0	25.06636937518699	0.0
29	1.3537863373175264E-4	0.0	0.0	25.671173421383596	0.0
30	1.3537863373175264E-4	0.0	0.0	26.3305350569741	0.0
31	1.3537863373175264E-4	0.0	0.0	26.915912269230198	0.0
32	1.3537863373175264E-4	0.0	0.0	27.460202066148707	0.0
33	1.3537863373175264E-4	0.0	0.0	28.00747019300932	0.0
34	1.3537863373175264E-4	0.0	0.0	28.572946746106847	0.0
35	1.3537863373175264E-4	0.0	0.0	29.180729122245552	0.0
36	1.3537863373175264E-4	0.0	0.0	29.72440971531227	0.0
37	1.3537863373175264E-4	0.0	0.0	30.330229101261864	0.0
38	1.3537863373175264E-4	0.0	0.0	31.003060910908676	0.0
39	1.3537863373175264E-4	0.0	0.0	32.04236268206734	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCATA	20	7.03394E-4	45.000004	36
GATCGTC	30	2.1659234E-6	45.000004	10
GGCCGTA	30	2.1659234E-6	45.000004	27
ATTACGT	30	2.1659234E-6	45.000004	11
ATTACGA	20	7.03394E-4	45.000004	25
GAATCGT	20	7.03394E-4	45.000004	12
ATTCGCG	20	7.03394E-4	45.000004	5
TAAGTCG	20	7.03394E-4	45.000004	2
CCTCGAC	30	2.1659234E-6	45.000004	29
CTAACGC	20	7.03394E-4	45.000004	44
CCCGTCA	20	7.03394E-4	45.000004	18
TCGGAAA	20	7.03394E-4	45.000004	36
TATCTCG	20	7.03394E-4	45.000004	44
TACGATA	20	7.03394E-4	45.000004	17
ACGGAGT	20	7.03394E-4	45.000004	27
TGCTCGA	20	7.03394E-4	45.000004	39
CTCGACT	20	7.03394E-4	45.000004	21
GTGCGTA	20	7.03394E-4	45.000004	21
AATCGAA	20	7.03394E-4	45.000004	15
TCGTTGC	25	3.8913488E-5	45.0	34
>>END_MODULE