##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935055.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2826084 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22465185040501 33.0 31.0 34.0 30.0 34.0 2 32.4020517436849 34.0 31.0 34.0 30.0 34.0 3 32.41563201943042 34.0 31.0 34.0 30.0 34.0 4 35.951552749316726 37.0 35.0 37.0 35.0 37.0 5 35.86167608606114 37.0 35.0 37.0 35.0 37.0 6 35.78197994114824 37.0 35.0 37.0 35.0 37.0 7 36.05285900914481 37.0 35.0 37.0 35.0 37.0 8 35.67749861646009 37.0 35.0 37.0 35.0 37.0 9 37.52656042778629 39.0 37.0 39.0 35.0 39.0 10 37.3694405403378 39.0 37.0 39.0 34.0 39.0 11 37.25632925277522 39.0 37.0 39.0 34.0 39.0 12 36.98494701502149 39.0 35.0 39.0 33.0 39.0 13 36.802254285435254 39.0 35.0 39.0 33.0 39.0 14 37.651690112537345 40.0 36.0 41.0 33.0 41.0 15 37.75303741856222 40.0 36.0 41.0 33.0 41.0 16 37.97188441674062 40.0 36.0 41.0 33.0 41.0 17 37.98394138320022 40.0 36.0 41.0 33.0 41.0 18 38.0030547570419 40.0 36.0 41.0 33.0 41.0 19 37.98326553633933 40.0 36.0 41.0 33.0 41.0 20 37.85349586211875 40.0 36.0 41.0 33.0 41.0 21 37.86789776949305 40.0 36.0 41.0 33.0 41.0 22 37.865459059249474 40.0 36.0 41.0 33.0 41.0 23 37.613464426393556 39.0 35.0 41.0 33.0 41.0 24 37.39710921543733 39.0 35.0 41.0 32.0 41.0 25 37.38075478294346 39.0 35.0 41.0 33.0 41.0 26 37.53621583788734 39.0 35.0 41.0 33.0 41.0 27 37.54206102861769 39.0 35.0 41.0 33.0 41.0 28 37.48104585709413 39.0 35.0 41.0 33.0 41.0 29 37.50305263396275 39.0 35.0 41.0 33.0 41.0 30 37.39070813181774 39.0 35.0 41.0 33.0 41.0 31 37.271640191869736 39.0 35.0 41.0 32.0 41.0 32 37.185856117511015 39.0 35.0 41.0 32.0 41.0 33 37.06216269580097 39.0 35.0 41.0 32.0 41.0 34 36.94947036252284 39.0 35.0 41.0 31.0 41.0 35 36.89060516247925 39.0 35.0 41.0 31.0 41.0 36 36.817840870971985 39.0 35.0 41.0 31.0 41.0 37 36.76438173812243 39.0 35.0 41.0 31.0 41.0 38 36.602321091658986 39.0 35.0 41.0 31.0 41.0 39 36.57136553619779 39.0 35.0 41.0 31.0 41.0 40 36.46400213157146 39.0 35.0 41.0 31.0 41.0 41 36.4082790886612 39.0 35.0 41.0 31.0 41.0 42 36.465957487463214 39.0 35.0 41.0 31.0 41.0 43 36.40753778019337 39.0 35.0 41.0 31.0 41.0 44 36.307823475876866 39.0 35.0 41.0 31.0 41.0 45 36.286496792027414 38.0 35.0 40.0 31.0 41.0 46 36.191950770040805 38.0 35.0 40.0 31.0 41.0 47 36.05428324140401 38.0 35.0 40.0 31.0 41.0 48 36.003795357816685 38.0 35.0 40.0 30.0 41.0 49 35.987481617673076 38.0 35.0 40.0 30.0 41.0 50 35.87743959485989 38.0 35.0 40.0 30.0 41.0 51 34.86971087908215 36.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 10.0 9 37.0 10 56.0 11 43.0 12 51.0 13 68.0 14 87.0 15 145.0 16 261.0 17 506.0 18 872.0 19 1578.0 20 3050.0 21 5301.0 22 7893.0 23 11335.0 24 14448.0 25 17395.0 26 20166.0 27 23493.0 28 27110.0 29 33440.0 30 42256.0 31 55662.0 32 76623.0 33 113756.0 34 218549.0 35 235916.0 36 205399.0 37 318871.0 38 564333.0 39 826725.0 40 647.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.509929641157164 20.769517112725595 24.31438697505099 10.406166271066253 2 29.2759875502639 28.066115515320845 24.59339495924396 18.064501975171297 3 30.312262480520747 28.31285269652282 25.40518965465995 15.969695168296482 4 27.761029042307307 24.06227132668385 29.50595240622713 18.67074722478171 5 28.421094348221782 27.877303010101613 23.711361728809194 19.990240912867417 6 26.021908761381475 35.68513887060682 23.764651015327214 14.528301352684492 7 77.72543208199049 5.472731879165658 11.647707569909457 5.154128468934398 8 77.45788872517589 7.831295885048002 8.481984258075839 6.228831131700261 9 72.79833154286992 6.17586030705386 9.857987236048185 11.167820914028033 10 45.184184192685 21.725150420157362 17.3437873750391 15.746878012118536 11 35.358998529413846 22.607608266420957 22.699289900795588 19.33410330336961 12 29.801520407744427 20.181636497712027 27.8021106237465 22.214732470797045 13 27.642030456278015 22.006989176542525 32.00003963081069 18.35094073636877 14 21.3094515237339 26.881118891016687 30.886484619706987 20.922944965542424 15 19.119884617725447 22.617940584922458 37.443473017787156 20.81870177956494 16 24.183817607686112 24.004806651182342 27.898356878281046 23.913018862850503 17 25.041116966091597 24.080600576628296 27.34228706577724 23.53599539150287 18 24.832382901569805 23.36537059761847 29.43306002227818 22.36918647853355 19 24.527331813208665 25.599805242873174 26.53771083945134 23.335152104466815 20 24.304160810506694 28.482132873615928 28.5317421562841 18.681964159593274 21 23.13473343325959 27.719805922258505 30.19421928010632 18.951241364375583 22 21.89106905527224 22.585068242840624 31.0058724369127 24.517990264974433 23 20.64046928541402 26.758864916966374 30.188345427807523 22.41232036981208 24 22.7715807456537 25.02547694972973 27.626178132001737 24.576764172614826 25 22.16370780203278 28.784813190266107 25.241040252165188 23.81043875553593 26 20.683037022254116 24.583947257052515 27.303540871396603 27.429474849296763 27 22.990151743543365 25.502214371547343 27.611705809169155 23.89592807574014 28 19.206364708196926 25.754259250609678 28.264941877169964 26.774434164023432 29 23.192658109242327 25.10385395480106 25.807123921298867 25.896364014657742 30 25.69396380291598 26.09497806859244 26.32692446509021 21.88413366340137 31 24.57149185940687 26.551227776669055 24.26201768949543 24.615262674428646 32 27.99233851506183 25.89371016572756 23.996491257867778 22.117460061342832 33 25.617886800250805 25.799268528465536 24.21127609795038 24.371568573333278 34 21.97089683109207 24.39644398397217 27.433544084323042 26.19911510061272 35 22.979465578517836 24.92731992396546 27.44444255726298 24.648771940253724 36 23.14825037047731 28.03851548644697 25.67085762489721 23.142376518178512 37 24.56961647282954 26.552890855331974 26.717252565741145 22.160240106097344 38 23.05568411979262 28.512988290510826 25.265278739060836 23.166048850635722 39 24.39955783338358 24.35840548263958 25.858219359367947 25.38381732460889 40 23.163218078443528 23.329773637301653 28.753816234761597 24.753192049493222 41 20.510642995749595 23.238658157365457 27.760002887387635 28.490695959497316 42 21.124000560492895 22.05107845343592 32.91087596830101 23.91404501777017 43 22.984985584292613 22.449474254834605 30.101865337336047 24.46367482353674 44 22.953457859002068 22.91393320226858 27.93363537672624 26.198973562003108 45 22.015410723814295 22.128712380806796 26.482935397532415 29.37294149784649 46 24.77612130424998 23.488190726107224 26.751823371138293 24.983864598504503 47 18.929090571971674 24.63479500255477 32.648923386566004 23.787191038907547 48 20.991308113983873 25.2221094631299 28.345335807428228 25.441246615457995 49 22.880353167138697 21.507110192053737 31.906694917773144 23.705841723034418 50 21.62890416562282 22.646000614277565 29.20337824353416 26.52171697656545 51 20.337081275715796 22.34806184104931 27.16196687713458 30.152890006100314 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1027.0 1 1886.0 2 2745.0 3 4383.0 4 6021.0 5 4563.5 6 3106.0 7 3338.0 8 3570.0 9 3770.5 10 3971.0 11 4155.0 12 4339.0 13 4757.0 14 5175.0 15 5121.5 16 5068.0 17 5187.5 18 5307.0 19 5855.0 20 6403.0 21 6826.0 22 7249.0 23 7680.5 24 8112.0 25 9860.5 26 15762.0 27 19915.0 28 21238.5 29 22562.0 30 28275.0 31 33988.0 32 38071.5 33 42155.0 34 51207.0 35 60259.0 36 64091.5 37 67924.0 38 74791.0 39 81658.0 40 88850.5 41 96043.0 42 112588.0 43 129133.0 44 146570.5 45 164008.0 46 242685.0 47 321362.0 48 311759.5 49 302157.0 50 301069.0 51 299981.0 52 260521.5 53 221062.0 54 200726.0 55 180390.0 56 167940.0 57 155490.0 58 148145.5 59 140801.0 60 125998.0 61 111195.0 62 99957.5 63 88720.0 64 78108.5 65 67497.0 66 58368.0 67 49239.0 68 41872.5 69 34506.0 70 29083.0 71 23660.0 72 20571.0 73 17482.0 74 12900.5 75 7061.0 76 5803.0 77 4924.5 78 4046.0 79 2734.5 80 1423.0 81 1170.5 82 918.0 83 646.5 84 375.0 85 279.5 86 184.0 87 115.0 88 46.0 89 45.5 90 45.0 91 35.5 92 26.0 93 17.5 94 9.0 95 5.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2826084.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.177245870906795 #Duplication Level Percentage of deduplicated Percentage of total 1 66.77597807964386 12.805793499036131 2 12.438312029035385 4.770651359997383 3 5.806505885925373 3.3405837307577504 4 3.096650333058894 2.3754089925318334 5 1.9610896479087985 1.8804149176418534 6 1.3659164649798399 1.5716709532822943 7 0.9886505057181003 1.327171567998665 8 0.7178020911822214 1.1012373751402014 9 0.5448896526894957 0.9404534557925525 >10 4.01360906937572 15.85936751692461 >50 1.1816175139663387 16.754757439473558 >100 1.093359470104378 29.744686478007104 >500 0.009221006797191155 1.1997973518297738 >1k 0.004892779116876939 1.9727402526746904 >5k 7.527352487502985E-4 0.9047380018760882 >10k+ 7.527352487502985E-4 3.450527107035438 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC 30505 1.0794088215353825 No Hit GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC 25979 0.9192578847620949 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG 25572 0.9048563312343157 No Hit GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC 13557 0.4797097326194126 No Hit CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC 7056 0.24967410735137383 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGACTTT 6512 0.2304248564444652 No Hit GAATGACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCT 5980 0.21160022136638545 No Hit CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT 5522 0.19539405056608364 TruSeq Adapter, Index 13 (95% over 23bp) GAATGATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTC 4343 0.15367554538364747 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTA 4321 0.15289708303079455 No Hit GAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT 3986 0.1410432244759887 No Hit TCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC 3353 0.11864473950526594 No Hit GAATGATCCGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC 3064 0.10841857496097074 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 2937 0.1039247241058652 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0615395720721677E-4 0.0 0.0 0.20618636954881736 0.0 2 1.0615395720721677E-4 0.0 0.0 1.1958950972441018 0.0 3 1.4153860960962236E-4 0.0 0.0 1.6726679037141146 0.0 4 1.4153860960962236E-4 0.0 0.0 2.4012025120272433 0.0 5 1.4153860960962236E-4 0.0 0.0 4.872502020463652 0.0 6 1.4153860960962236E-4 0.0 0.0 5.761187565550069 0.0 7 1.4153860960962236E-4 0.0 0.0 6.8871625896470166 0.0 8 1.4153860960962236E-4 0.0 0.0 8.421228809900908 0.0 9 1.4153860960962236E-4 0.0 0.0 8.905750855247048 0.0 10 1.4153860960962236E-4 0.0 0.0 11.033288465594087 0.0 11 1.7692326201202796E-4 0.0 0.0 12.78949953363028 0.0 12 1.7692326201202796E-4 0.0 0.0 15.3359560437694 0.0 13 2.476925668168391E-4 0.0 0.0 15.924438197873807 0.0 14 2.476925668168391E-4 0.0 0.0 16.18837232014335 0.0 15 2.476925668168391E-4 0.0 0.0 16.751660601737246 0.0 16 2.476925668168391E-4 0.0 0.0 17.591692249770354 0.0 17 2.476925668168391E-4 0.0 0.0 18.488233187690103 0.0 18 2.476925668168391E-4 0.0 0.0 19.518103495862118 0.0 19 2.476925668168391E-4 0.0 0.0 20.44698600607767 0.0 20 2.476925668168391E-4 0.0 0.0 21.135182110652053 0.0 21 2.476925668168391E-4 0.0 0.0 21.90706291815813 0.0 22 2.476925668168391E-4 0.0 0.0 22.748934568116162 0.0 23 2.476925668168391E-4 0.0 0.0 23.462996853596707 0.0 24 2.476925668168391E-4 0.0 0.0 24.07196672144211 0.0 25 2.830772192192447E-4 0.0 0.0 24.632601154105824 0.0 26 3.184618716216503E-4 0.0 0.0 25.156258625009023 0.0 27 3.184618716216503E-4 0.0 0.0 25.816359315575898 0.0 28 3.538465240240559E-4 0.0 0.0 26.35445372465928 3.538465240240559E-5 29 3.538465240240559E-4 0.0 0.0 26.931258943470894 3.538465240240559E-5 30 3.538465240240559E-4 0.0 0.0 27.594933483930415 3.538465240240559E-5 31 3.538465240240559E-4 0.0 0.0 28.117281722694724 3.538465240240559E-5 32 3.538465240240559E-4 0.0 0.0 28.62246840504387 3.538465240240559E-5 33 3.538465240240559E-4 0.0 0.0 29.138659714290164 3.538465240240559E-5 34 3.538465240240559E-4 0.0 0.0 29.696852605938112 3.538465240240559E-5 35 3.538465240240559E-4 0.0 0.0 30.2733039782257 3.538465240240559E-5 36 3.8923117642646147E-4 0.0 0.0 30.771909115228 3.538465240240559E-5 37 3.8923117642646147E-4 0.0 0.0 31.318708148802372 3.538465240240559E-5 38 4.2461582882886707E-4 0.0 0.0 31.956091892526903 3.538465240240559E-5 39 4.2461582882886707E-4 0.0 0.0 32.9856791234797 3.538465240240559E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATCCG 20 7.035036E-4 45.0 1 ACTCGAT 20 7.035036E-4 45.0 33 CGTATTA 25 3.8922568E-5 44.999996 14 CGCGATC 25 3.8922568E-5 44.999996 16 TACGCTA 30 2.166631E-6 44.999996 13 ATCGCAA 30 2.166631E-6 44.999996 32 TATCGCG 70 0.0 41.785713 1 TCGGTAA 40 3.4606092E-7 39.375 11 GCGATAT 70 0.0 38.571426 9 ACCGTAT 35 6.2515264E-6 38.571426 31 TATCGAT 35 6.2515264E-6 38.571426 15 TACGGCT 3900 0.0 38.480774 7 CCGATGA 1645 0.0 38.161095 18 TGATCCG 785 0.0 38.121017 4 GTATACG 155 0.0 37.741936 1 CGTTTTT 2125 0.0 37.69412 1 ACCGCGT 30 1.1403437E-4 37.499996 23 ACGTCGA 30 1.1403437E-4 37.499996 29 TACAACG 115 0.0 37.173916 1 CGTAAGG 510 0.0 37.058823 2 >>END_MODULE