FastQCFastQC Report
Sat 14 Jan 2017
SRR2935054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970753
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC208292.145653940806776No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG201342.074060033808806No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC182901.8841044014285817No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC80550.8297682314656767No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCTTTCAT56930.5864519604884043No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC48550.5001272208275431No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCT46030.47416799123979014No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTC33360.34365075359025415No Hit
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT30750.3167644086600814No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTA28950.2982221018116864No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT25220.259798321509179No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC23920.2464066554520048No Hit
TCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC20400.2101461442818101No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG19840.20437742659564279No Hit
ACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC16090.1657476206614865No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG10300.10610320029914921No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG9820.10115858513957722No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9750.1004374954288063No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG9730.10023146979715747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG406.8139343E-945.00000445
TATCATG302.1651522E-645.00000411
ACTATTC207.032743E-445.00000442
CGGAACG207.032743E-445.00000427
GGTACAC207.032743E-445.0000048
TACCCCG207.032743E-445.00000424
CGTATTA302.1651522E-645.00000441
GTCGATG207.032743E-445.00000414
GTCGAGT207.032743E-445.00000435
CCTAGTC207.032743E-445.00000421
CGAAAAC207.032743E-445.00000444
CGCGGAT207.032743E-445.0000044
TCCGATT207.032743E-445.00000410
GAGTCGA207.032743E-445.00000430
ACGCACC406.8139343E-945.00000418
GACGTGA207.032743E-445.00000411
CTATACG302.1651522E-645.0000041
ATGTGCG406.8139343E-945.0000041
TGGCGAT207.032743E-445.00000441
TAAACGG1200.045.0000042
Produced by FastQC (version 0.11.5)