##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935054.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	970753
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12909051015037	33.0	31.0	34.0	30.0	34.0
2	32.30658468220031	34.0	31.0	34.0	30.0	34.0
3	32.33059181892819	34.0	31.0	34.0	30.0	34.0
4	35.90162791152847	37.0	35.0	37.0	35.0	37.0
5	35.79560094071304	37.0	35.0	37.0	35.0	37.0
6	35.70153684819928	37.0	35.0	37.0	35.0	37.0
7	35.950410660590286	37.0	35.0	37.0	35.0	37.0
8	35.378598881486845	37.0	35.0	37.0	33.0	37.0
9	37.28274339610591	39.0	37.0	39.0	34.0	39.0
10	37.261503183611076	39.0	37.0	39.0	34.0	39.0
11	37.19898264543092	39.0	37.0	39.0	34.0	39.0
12	36.91932963379974	39.0	35.0	39.0	33.0	39.0
13	36.66755086000249	39.0	35.0	39.0	33.0	39.0
14	37.73815481384039	40.0	36.0	41.0	33.0	41.0
15	37.752577638183965	40.0	36.0	41.0	33.0	41.0
16	37.96564522592256	40.0	36.0	41.0	33.0	41.0
17	37.85134838625273	40.0	36.0	41.0	33.0	41.0
18	37.90826399712388	40.0	36.0	41.0	33.0	41.0
19	37.86917990467194	40.0	36.0	41.0	34.0	41.0
20	37.64028954842272	40.0	35.0	41.0	33.0	41.0
21	37.71042788433309	39.0	35.0	41.0	33.0	41.0
22	37.66635797159525	39.0	35.0	41.0	33.0	41.0
23	37.320669624507985	39.0	35.0	41.0	32.0	41.0
24	37.12884018900792	39.0	35.0	41.0	32.0	41.0
25	37.15012160662908	39.0	35.0	41.0	32.0	41.0
26	37.283266701210295	39.0	35.0	41.0	33.0	41.0
27	37.27053019666177	39.0	35.0	41.0	33.0	41.0
28	37.22910256264982	39.0	35.0	41.0	32.0	41.0
29	37.30342939965161	39.0	35.0	41.0	33.0	41.0
30	37.257133379963804	39.0	35.0	41.0	33.0	41.0
31	37.21634751579444	39.0	35.0	41.0	33.0	41.0
32	36.95540884241409	39.0	35.0	41.0	32.0	41.0
33	36.82651302648563	39.0	35.0	41.0	31.0	41.0
34	36.776128428137746	39.0	35.0	41.0	31.0	41.0
35	36.69890384062681	39.0	35.0	41.0	31.0	41.0
36	36.58517872208481	39.0	35.0	41.0	31.0	41.0
37	36.54355021308201	39.0	35.0	41.0	31.0	41.0
38	36.49242289233204	39.0	35.0	41.0	31.0	41.0
39	36.436369498729334	39.0	35.0	41.0	31.0	41.0
40	36.396218193505455	39.0	35.0	41.0	31.0	41.0
41	36.27165818699505	39.0	35.0	41.0	30.0	41.0
42	36.33148184965692	39.0	35.0	41.0	31.0	41.0
43	36.23557176748359	39.0	35.0	40.0	31.0	41.0
44	36.1858042159025	39.0	35.0	40.0	30.0	41.0
45	36.186352244082684	38.0	35.0	40.0	30.0	41.0
46	36.04063031481746	38.0	35.0	40.0	30.0	41.0
47	35.80809948565701	38.0	35.0	40.0	30.0	41.0
48	35.83971514896168	38.0	35.0	40.0	30.0	41.0
49	35.79915385273082	38.0	35.0	40.0	29.0	41.0
50	35.72400909397138	38.0	35.0	40.0	29.0	41.0
51	34.81382287770422	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	15.0
10	21.0
11	16.0
12	8.0
13	12.0
14	25.0
15	29.0
16	67.0
17	133.0
18	297.0
19	643.0
20	1246.0
21	2271.0
22	3853.0
23	5519.0
24	6722.0
25	7518.0
26	7827.0
27	8221.0
28	9134.0
29	11460.0
30	14438.0
31	18923.0
32	25691.0
33	36090.0
34	68163.0
35	104524.0
36	69516.0
37	105988.0
38	189912.0
39	272274.0
40	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.996664445023605	20.748841363354014	23.114324653130097	10.140169538492284
2	27.5914676544909	33.88266634251968	22.376443853379797	16.14942214960963
3	27.13069132930828	33.829614742370104	24.070798648059803	14.968895280261819
4	26.075015992739658	23.91926679598209	33.430440081050484	16.57527713022777
5	30.802686162185438	26.51580783165234	22.66199537884508	20.019510627317143
6	23.151821318090185	38.9788133541694	23.62052963009128	14.248835697649145
7	71.09934246919659	6.4038947085406885	17.079009799609167	5.417753022653549
8	69.35688068952658	12.626486861230404	10.11791877027421	7.898713678968801
9	64.69750801697239	8.093201875245299	10.618561055180875	16.590729052601432
10	43.94351601282715	23.149967087405344	17.74539970517732	15.16111719459018
11	36.68605711236535	21.619711708333632	22.578658010843128	19.11557316845789
12	29.840031398306266	19.73045666611383	27.20702382583417	23.222488109745733
13	24.11148871031045	26.14568278439521	32.49693794404962	17.245890561244725
14	18.77789715818545	33.030853368467575	26.05925503191852	22.131994441428457
15	15.114658414653368	23.344043232418546	41.20131485558118	20.339983497346907
16	16.406748163539024	24.697734645167206	30.967300641872857	27.928216549420913
17	17.087971914585896	24.94506841596163	30.109203886055464	27.857755783397014
18	18.51500845220154	25.150166932268043	30.616129952727416	25.718694662803
19	21.30006294083047	27.09381274124314	25.67233889568201	25.933785422244384
20	24.932088801167755	26.06996836476426	29.77441223462611	19.223530599441876
21	21.222288264883034	32.03307123439227	26.69216577234374	20.052474728380957
22	18.4465049296783	24.325343315961938	28.227571792206668	29.00057996215309
23	19.08878983634354	29.89843966487871	27.24709581118987	23.765674687587882
24	23.073325552431978	23.49207264875823	27.44282016125626	25.99178163755353
25	18.141690007653853	32.37054121903306	24.866366624671777	24.621402148641312
26	17.73272912883092	26.454206167789334	28.619123505155276	27.19394119822447
27	22.939408891860236	28.768801126548155	25.18230693080526	23.10948305078635
28	15.609634994689689	27.08176024179168	31.399645429887933	25.908959333630698
29	20.432695031588878	22.256536935760177	29.116366367139733	28.194401665511204
30	22.40848083910119	27.53439855452417	27.723530084377796	22.33359052199684
31	23.634230334595927	23.936675961856416	23.015638375570305	29.413455327977356
32	25.312103078744023	27.027781526299687	24.78787085901357	22.872244535942716
33	22.76057864358905	24.275176074655448	26.07491297992383	26.88933230183167
34	20.547657334048928	22.234286167542102	30.00485190362533	27.213204594783637
35	21.0208982099463	21.85324176180759	27.101641715245794	30.024218313000322
36	20.436506505774382	26.609240455605082	25.442929354841038	27.511323683779498
37	22.461532439250767	23.107319781654034	32.65681383420912	21.774333944886084
38	18.48214736395355	25.309424745532596	26.645397953959453	29.563029936554408
39	21.109695257186946	23.9395603206995	33.43002802978718	21.52071639232637
40	20.851751166362607	20.02435222966089	32.80916978881343	26.314726815163077
41	18.318769038056022	26.12992182357407	27.133060624072243	28.418248514297662
42	19.922987618889664	22.420327312921	28.180597948190734	29.4760871199986
43	22.790040308914833	24.490884910991774	25.390186793138938	27.32888798695446
44	21.30026896646212	22.23912776988585	29.374207445148247	27.086395818503778
45	19.442020781805464	20.823731680458366	27.721160789613837	32.01308674812233
46	24.932603865246875	25.286864938867044	27.3299181151127	22.45061308077338
47	17.337984018591754	22.633357816045894	36.453866225497116	23.57479193986524
48	21.250101725155627	22.266735204526793	28.674853438516283	27.808309631801293
49	20.02806069103057	18.71124786634705	35.67313209436386	25.587559348258516
50	20.123553571299805	21.19117839450406	31.291275947640646	27.393992086555492
51	19.132364257437267	21.060661156854525	26.580602892805892	33.22637169290231
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1185.0
1	1336.5
2	1488.0
3	1885.0
4	2282.0
5	1828.5
6	1375.0
7	1374.0
8	1373.0
9	1516.5
10	1660.0
11	1747.0
12	1834.0
13	1913.5
14	1993.0
15	2137.5
16	2282.0
17	2575.0
18	2868.0
19	2613.0
20	2358.0
21	2569.5
22	2781.0
23	2758.5
24	2736.0
25	3671.0
26	5325.5
27	6045.0
28	7069.0
29	8093.0
30	9125.0
31	10157.0
32	12299.5
33	14442.0
34	15817.5
35	17193.0
36	18907.5
37	20622.0
38	22235.0
39	23848.0
40	28869.0
41	33890.0
42	42746.5
43	51603.0
44	59971.0
45	68339.0
46	112305.5
47	156272.0
48	134725.5
49	113179.0
50	110048.5
51	106918.0
52	88590.5
53	70263.0
54	61119.0
55	51975.0
56	47883.5
57	43792.0
58	40724.0
59	37656.0
60	33563.0
61	29470.0
62	28180.5
63	26891.0
64	23058.5
65	19226.0
66	15126.5
67	11027.0
68	8936.0
69	6845.0
70	5928.5
71	5012.0
72	4044.0
73	3076.0
74	2461.0
75	1585.0
76	1324.0
77	906.5
78	489.0
79	346.5
80	204.0
81	123.5
82	43.0
83	84.0
84	125.0
85	72.5
86	20.0
87	29.5
88	39.0
89	21.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	970753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.753938711824176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.54021908169501	13.341853483229812
2	12.3331349021386	4.872559819630111
3	5.778903651905419	3.4246832568382963
4	3.20980176714455	2.5362490954111343
5	2.0701627783537933	2.0446934323550248
6	1.3747523624607076	1.6294064347190589
7	0.9629803485832313	1.3315858350622962
8	0.6843112676585925	1.0814274272910835
9	0.5387712228002792	0.9578568343402155
>10	3.4271281865426992	13.66788069967706
>50	1.0976464600136935	16.02166151126014
>100	0.9632084930087048	26.71207274187848
>500	0.010544154274862669	1.615590349933373
>1k	0.005799284851174468	3.159841862136819
>5k	0.001054415427486267	1.4317756804540716
>10k+	0.0015816231412294002	6.170861535783031
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	20829	2.145653940806776	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	20134	2.074060033808806	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC	18290	1.8841044014285817	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	8055	0.8297682314656767	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCTTTCAT	5693	0.5864519604884043	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	4855	0.5001272208275431	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCT	4603	0.47416799123979014	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTC	3336	0.34365075359025415	No Hit
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT	3075	0.3167644086600814	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTA	2895	0.2982221018116864	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT	2522	0.259798321509179	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	2392	0.2464066554520048	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	2040	0.2101461442818101	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	1984	0.20437742659564279	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	1609	0.1657476206614865	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	1030	0.10610320029914921	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	982	0.10115858513957722	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	975	0.1004374954288063	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	973	0.10023146979715747	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0301281582441671E-4	0.0	0.0	0.33221633103374393	0.0
2	1.0301281582441671E-4	0.0	0.0	2.1134109294537335	0.0
3	1.0301281582441671E-4	0.0	0.0	2.8329554479872843	0.0
4	1.0301281582441671E-4	0.0	0.0	3.8630836062314513	0.0
5	1.0301281582441671E-4	0.0	0.0	7.8445289378451575	0.0
6	1.0301281582441671E-4	0.0	0.0	8.956140233406439	0.0
7	1.0301281582441671E-4	0.0	0.0	10.502465611746757	0.0
8	1.0301281582441671E-4	0.0	0.0	12.394811038441292	0.0
9	1.0301281582441671E-4	0.0	0.0	12.92512101430539	0.0
10	1.0301281582441671E-4	0.0	0.0	16.42158200901774	0.0
11	1.0301281582441671E-4	0.0	0.0	18.372335702284722	0.0
12	1.0301281582441671E-4	0.0	0.0	22.330397124706284	0.0
13	1.0301281582441671E-4	0.0	0.0	23.03974337447322	0.0
14	1.0301281582441671E-4	0.0	0.0	23.358465026633965	0.0
15	1.0301281582441671E-4	0.0	0.0	24.12199601752454	0.0
16	1.0301281582441671E-4	0.0	0.0	25.08382667887712	0.0
17	1.0301281582441671E-4	0.0	0.0	26.126316375020217	0.0
18	1.0301281582441671E-4	0.0	0.0	27.382454651183153	1.0301281582441671E-4
19	1.0301281582441671E-4	0.0	0.0	28.758293819334064	1.0301281582441671E-4
20	1.0301281582441671E-4	0.0	0.0	29.4452862880671	1.0301281582441671E-4
21	1.0301281582441671E-4	0.0	0.0	30.217058304223627	1.0301281582441671E-4
22	1.0301281582441671E-4	0.0	0.0	31.045384356267764	1.0301281582441671E-4
23	1.0301281582441671E-4	0.0	0.0	31.809533424053285	1.0301281582441671E-4
24	1.0301281582441671E-4	0.0	0.0	32.44965506158621	1.0301281582441671E-4
25	1.0301281582441671E-4	0.0	0.0	32.95709619233729	1.0301281582441671E-4
26	4.1205126329766685E-4	0.0	0.0	33.41086764604385	1.0301281582441671E-4
27	4.1205126329766685E-4	0.0	0.0	33.921502174085475	1.0301281582441671E-4
28	4.1205126329766685E-4	0.0	0.0	34.387841191322615	1.0301281582441671E-4
29	4.1205126329766685E-4	0.0	0.0	34.88467200204377	1.0301281582441671E-4
30	4.1205126329766685E-4	0.0	0.0	35.44804909178751	1.0301281582441671E-4
31	5.150640791220835E-4	0.0	0.0	35.9707361192806	1.0301281582441671E-4
32	5.150640791220835E-4	0.0	0.0	36.45046680257491	1.0301281582441671E-4
33	5.150640791220835E-4	0.0	0.0	36.9195871658393	1.0301281582441671E-4
34	7.21089710770917E-4	0.0	0.0	37.40642573342549	1.0301281582441671E-4
35	7.21089710770917E-4	0.0	0.0	37.922519940705826	1.0301281582441671E-4
36	7.21089710770917E-4	0.0	0.0	38.37752754820227	1.0301281582441671E-4
37	7.21089710770917E-4	0.0	0.0	38.82110073314221	1.0301281582441671E-4
38	7.21089710770917E-4	0.0	0.0	39.30433385217455	1.0301281582441671E-4
39	7.21089710770917E-4	0.0	0.0	39.78694889431194	1.0301281582441671E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	40	6.8139343E-9	45.000004	45
TATCATG	30	2.1651522E-6	45.000004	11
ACTATTC	20	7.032743E-4	45.000004	42
CGGAACG	20	7.032743E-4	45.000004	27
GGTACAC	20	7.032743E-4	45.000004	8
TACCCCG	20	7.032743E-4	45.000004	24
CGTATTA	30	2.1651522E-6	45.000004	41
GTCGATG	20	7.032743E-4	45.000004	14
GTCGAGT	20	7.032743E-4	45.000004	35
CCTAGTC	20	7.032743E-4	45.000004	21
CGAAAAC	20	7.032743E-4	45.000004	44
CGCGGAT	20	7.032743E-4	45.000004	4
TCCGATT	20	7.032743E-4	45.000004	10
GAGTCGA	20	7.032743E-4	45.000004	30
ACGCACC	40	6.8139343E-9	45.000004	18
GACGTGA	20	7.032743E-4	45.000004	11
CTATACG	30	2.1651522E-6	45.000004	1
ATGTGCG	40	6.8139343E-9	45.000004	1
TGGCGAT	20	7.032743E-4	45.000004	41
TAAACGG	120	0.0	45.000004	2
>>END_MODULE