##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935053.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6121140
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.377657919929945	33.0	31.0	34.0	31.0	34.0
2	32.58661017392185	34.0	31.0	34.0	31.0	34.0
3	32.508809633499645	34.0	31.0	34.0	31.0	34.0
4	36.07232770366304	37.0	35.0	37.0	35.0	37.0
5	36.00600313013589	37.0	35.0	37.0	35.0	37.0
6	35.9546349209461	37.0	35.0	37.0	35.0	37.0
7	36.18903080145202	37.0	36.0	37.0	35.0	37.0
8	35.99831926732602	37.0	36.0	37.0	35.0	37.0
9	37.746957592866686	39.0	38.0	39.0	35.0	39.0
10	37.606255370731596	39.0	37.0	39.0	35.0	39.0
11	37.522719297385784	39.0	37.0	39.0	35.0	39.0
12	37.296331565688746	39.0	37.0	39.0	35.0	39.0
13	37.200731072970065	39.0	37.0	39.0	34.0	39.0
14	38.24265316591354	40.0	38.0	41.0	33.0	41.0
15	38.340567770055905	40.0	38.0	41.0	33.0	41.0
16	38.41301620286417	40.0	38.0	41.0	34.0	41.0
17	38.42365637773356	40.0	38.0	41.0	34.0	41.0
18	38.32492362533777	40.0	37.0	41.0	34.0	41.0
19	38.20316640364376	40.0	37.0	41.0	34.0	41.0
20	38.06862136791513	40.0	36.0	41.0	34.0	41.0
21	37.9986979549561	40.0	35.0	41.0	34.0	41.0
22	37.98724142888416	40.0	35.0	41.0	34.0	41.0
23	37.814466096184695	40.0	35.0	41.0	34.0	41.0
24	37.59597787340267	39.0	35.0	41.0	33.0	41.0
25	37.548074541670346	39.0	35.0	41.0	33.0	41.0
26	37.68991233659089	39.0	35.0	41.0	34.0	41.0
27	37.7332274706999	40.0	35.0	41.0	34.0	41.0
28	37.67282957096227	40.0	35.0	41.0	34.0	41.0
29	37.65363494381766	40.0	35.0	41.0	34.0	41.0
30	37.53423806676534	40.0	35.0	41.0	33.0	41.0
31	37.373364111913794	40.0	35.0	41.0	33.0	41.0
32	37.19854422542206	40.0	35.0	41.0	33.0	41.0
33	36.98679951773689	39.0	35.0	41.0	32.0	41.0
34	36.70518400167289	39.0	35.0	41.0	31.0	41.0
35	36.513127620018494	39.0	35.0	41.0	31.0	41.0
36	36.433908226245435	39.0	35.0	41.0	30.0	41.0
37	36.384252769908876	39.0	35.0	41.0	30.0	41.0
38	36.298513512188904	39.0	35.0	41.0	30.0	41.0
39	36.19487399405993	39.0	35.0	41.0	30.0	41.0
40	36.12192222363808	39.0	35.0	41.0	30.0	41.0
41	35.99999444547911	39.0	35.0	41.0	30.0	41.0
42	35.955695507699545	39.0	35.0	41.0	30.0	41.0
43	35.885941180891145	39.0	35.0	41.0	30.0	41.0
44	35.749711001545464	38.0	35.0	41.0	29.0	41.0
45	35.71290364213202	38.0	35.0	41.0	29.0	41.0
46	35.63764560196303	38.0	35.0	40.0	29.0	41.0
47	35.57737578294239	38.0	35.0	40.0	29.0	41.0
48	35.47814099987911	38.0	35.0	40.0	29.0	41.0
49	35.410307230352515	38.0	35.0	40.0	28.0	41.0
50	35.3389685581444	37.0	35.0	40.0	28.0	41.0
51	34.35611487402674	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	36.0
9	81.0
10	106.0
11	92.0
12	89.0
13	121.0
14	179.0
15	264.0
16	518.0
17	993.0
18	1667.0
19	2954.0
20	5012.0
21	8086.0
22	12277.0
23	18830.0
24	29031.0
25	48378.0
26	71057.0
27	83780.0
28	86029.0
29	87305.0
30	94906.0
31	113139.0
32	144222.0
33	212273.0
34	441103.0
35	451571.0
36	435672.0
37	661205.0
38	1184324.0
39	1924230.0
40	1605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.00465926281706	21.052777750549733	24.418310968218336	17.52425201841487
2	36.504147920158665	22.426802850449427	24.363549926974386	16.705499302417522
3	29.394393854739477	22.526245111204776	31.465364294886243	16.6139967391695
4	26.582597359315425	23.999320388032295	31.562944157460866	17.85513809519142
5	24.658396965271177	27.358220854285314	29.468563045445784	18.51481913499773
6	25.922638593464615	30.773728423136866	27.749716556066357	15.553916427332162
7	76.7722515740532	5.150968610422241	12.717287956165027	5.359491859359531
8	78.8402323750151	3.4539481207748883	12.559523226065734	5.146296278144267
9	72.50211561898601	5.569779485520671	14.920325298882235	7.007779596611089
10	42.048409283238094	21.615091306521332	21.107359086706072	15.229140323534503
11	30.91984499619352	20.659190935021908	28.65833815269705	19.762625916087526
12	27.733461414050325	18.462753670067993	32.619773440894996	21.184011474986686
13	25.03946977197058	19.05184328409414	35.44632209032958	20.4623648536057
14	19.679046713520684	21.50707221203893	37.226300983150196	21.587580091290185
15	18.718049252263466	21.600861930947506	37.92102451504132	21.760064301747715
16	24.116700483896793	22.467138474205786	33.022100458411344	20.39406058348608
17	24.5386480296154	23.16526006593543	31.091447018039126	21.204644886410048
18	24.580944072509368	23.080602632842904	31.663546332872635	20.674906961775093
19	23.883083869998075	25.769529858817148	29.01611464531117	21.33127162587361
20	23.247744701150438	27.79776316176398	28.574726276477914	20.379765860607666
21	23.015990485432454	25.655694854226503	31.460038489562404	19.868276170778646
22	22.392087094887554	22.3928712625426	32.399504013958186	22.815537628611665
23	21.588838026903485	23.615045563408124	32.31139951054869	22.484716899139702
24	22.231741146257068	23.7062867374378	31.411779505124866	22.65019261118027
25	24.203154967865462	24.37766494476519	28.83493924334356	22.584240844025786
26	21.36450073025613	23.686094420320398	29.85569681464564	25.093708034777833
27	21.459417690168824	22.191879290458967	31.868442806405344	24.480260212966865
28	21.00620145920531	22.953240736202734	30.31797998412061	25.722577820471347
29	22.463707740714963	23.74281588070196	29.775058240785214	24.018418137797862
30	24.61425486102262	21.851109433863627	31.871007688110385	21.66362801700337
31	24.670371858836752	22.052166753251846	31.489003682320615	21.788457705590787
32	26.099533746981773	21.151239801736278	30.455879133625434	22.293347317656515
33	25.062782422882012	20.979980853239756	30.608710142228407	23.34852658164982
34	22.531766958442383	20.910892415465092	32.501788882463075	24.055551743629454
35	21.36126603867907	23.69677870462038	30.97828182332049	23.963673433380055
36	22.757345853876892	24.090365520148207	28.910889148099866	24.241399477875035
37	22.264414798550597	24.051304168831297	29.619940076521694	24.064340956096412
38	22.132021159457224	22.957815047523827	29.751696579395343	25.15846721362361
39	21.55920302427326	22.354724773489906	28.80282104313902	27.283251159097816
40	23.560758290122426	20.760626288567163	30.097645209879204	25.580970211431204
41	20.9943899339012	22.300290468768893	29.95589056940374	26.74942902792617
42	20.745678092642876	23.041018503089294	31.892768340537874	24.32053506372996
43	21.48877496675456	21.66648696157905	32.28981856320882	24.554919508457576
44	23.458800158140477	21.23754725426963	29.668983228614277	25.634669358975614
45	22.016111377945936	21.250535031056305	29.291635218276333	27.441718372721418
46	22.304619727697784	21.55479208121363	29.45368019682608	26.686907994262505
47	20.946261644072838	22.705149694337983	31.40977007550881	24.938818586080373
48	21.08484694027583	22.560176699111604	31.447295765167926	24.90768059544464
49	22.550881045034096	20.803559467680856	31.563859019725083	25.081700467559966
50	20.931166416713225	21.4025001878735	31.879143427531474	25.7871899678818
51	20.571249799873883	21.04233851864195	30.999356329049814	27.387055352434352
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3079.0
1	4772.5
2	6466.0
3	58548.0
4	110630.0
5	72199.5
6	33769.0
7	33376.0
8	32983.0
9	33265.5
10	33548.0
11	32647.5
12	31747.0
13	29963.5
14	28180.0
15	26640.5
16	25101.0
17	23574.0
18	22047.0
19	21401.5
20	20756.0
21	20911.0
22	21066.0
23	22985.0
24	24904.0
25	27691.5
26	36432.5
27	42386.0
28	48286.0
29	54186.0
30	62442.5
31	70699.0
32	81533.0
33	92367.0
34	108499.5
35	124632.0
36	137650.5
37	150669.0
38	162877.0
39	175085.0
40	183594.0
41	192103.0
42	209822.5
43	227542.0
44	262294.0
45	297046.0
46	388479.5
47	479913.0
48	533081.0
49	586249.0
50	591188.5
51	596128.0
52	527517.0
53	458906.0
54	414275.5
55	369645.0
56	357615.5
57	345586.0
58	336413.0
59	327240.0
60	301859.0
61	276478.0
62	252048.5
63	227619.0
64	206547.5
65	185476.0
66	161144.0
67	136812.0
68	117536.0
69	98260.0
70	83238.5
71	68217.0
72	56960.0
73	45703.0
74	39147.5
75	25079.5
76	17567.0
77	13003.5
78	8440.0
79	6625.0
80	4810.0
81	3667.0
82	2524.0
83	1653.0
84	782.0
85	596.5
86	411.0
87	307.5
88	204.0
89	128.5
90	53.0
91	39.5
92	26.0
93	20.5
94	15.0
95	12.0
96	9.0
97	6.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6121140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.46280015598729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.24725317449474	14.579183033727167
2	10.740389399087414	4.395568837420203
3	4.447652427957936	2.7303426838978475
4	2.2969733462784263	1.8801002619409934
5	1.4207611533448494	1.4536375775142834
6	0.9955737894331916	1.2223336496226223
7	0.7063880258924636	1.01182739044939
8	0.5616341812513262	0.9194086409373947
9	0.45229762152366937	0.8329748256240499
>10	4.677688164088589	22.787711074559514
>50	1.922321404249145	28.2484600687123
>100	0.5203024004674364	13.846969347823082
>500	0.006361084625627957	0.8640158931464028
>1k	0.0033436466575190466	1.1276011285182268
>5k	5.708665025032169E-4	0.8571737748458855
>10k+	4.893141450027573E-4	3.242691811260652
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66831	1.0918064282143523	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT	38514	0.6291965222164498	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	28498	0.46556687153046655	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	23890	0.3902867766461803	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	21609	0.3530224762054127	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT	14972	0.2445949610693433	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	9712	0.15866325553736724	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC	8694	0.14203236651996196	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT	7657	0.12509107780576822	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	6872	0.11226666928055885	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	6833	0.11162953306083508	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7970508761439862E-4	0.0	0.0	0.13074361965254838	0.0
2	1.7970508761439862E-4	0.0	0.0	0.4777704806620989	0.0
3	1.7970508761439862E-4	0.0	0.0	1.1029644804725918	0.0
4	1.7970508761439862E-4	0.0	0.0	1.6896852547074566	0.0
5	1.7970508761439862E-4	0.0	0.0	3.1900757048523642	0.0
6	1.7970508761439862E-4	0.0	0.0	5.166619289870841	0.0
7	1.7970508761439862E-4	0.0	0.0	6.748514165661952	0.0
8	1.7970508761439862E-4	0.0	0.0	8.59088339753706	0.0
9	1.7970508761439862E-4	0.0	0.0	9.898482962324012	0.0
10	1.7970508761439862E-4	0.0	0.0	11.44737744929866	0.0
11	1.7970508761439862E-4	0.0	0.0	12.998363050020094	0.0
12	1.7970508761439862E-4	0.0	0.0	14.026374172131336	0.0
13	1.7970508761439862E-4	0.0	0.0	14.528486523752111	0.0
14	1.7970508761439862E-4	0.0	0.0	14.917450017480403	0.0
15	1.7970508761439862E-4	0.0	0.0	15.218243660494613	0.0
16	1.7970508761439862E-4	0.0	0.0	15.635077779629285	0.0
17	1.7970508761439862E-4	0.0	0.0	16.099827809852414	0.0
18	2.1237873990792564E-4	0.0	0.0	16.619877996582336	0.0
19	2.1237873990792564E-4	0.0	0.0	16.970498959344173	0.0
20	2.1237873990792564E-4	0.0	0.0	17.347111812505513	0.0
21	2.1237873990792564E-4	0.0	0.0	17.78274961853511	0.0
22	2.2871556605468915E-4	0.0	0.0	18.2724296454582	0.0
23	2.613892183482162E-4	0.0	0.0	18.72651172820749	0.0
24	2.613892183482162E-4	0.0	0.0	19.107649882211483	0.0
25	2.613892183482162E-4	0.0	0.0	19.443812753833438	0.0
26	2.613892183482162E-4	0.0	0.0	19.769928477375128	0.0
27	2.613892183482162E-4	0.0	0.0	20.170785180538267	0.0
28	2.940628706417432E-4	1.6336826146763512E-5	0.0	20.522108626824416	0.0
29	3.4307334908203373E-4	1.6336826146763512E-5	0.0	20.912787487298118	0.0
30	3.4307334908203373E-4	1.6336826146763512E-5	0.0	21.384954436591876	0.0
31	3.5941017522879724E-4	4.901047844029053E-5	0.0	21.740492784023893	0.0
32	3.5941017522879724E-4	6.534730458705405E-5	0.0	22.14174157101455	0.0
33	3.9208382752232426E-4	6.534730458705405E-5	0.0	22.526571847727713	0.0
34	4.0842065366908777E-4	6.534730458705405E-5	0.0	22.932492966996342	0.0
35	4.247574798158513E-4	6.534730458705405E-5	0.0	23.348183508300743	0.0
36	4.410943059626148E-4	6.534730458705405E-5	0.0	23.72969740930611	0.0
37	4.574311321093783E-4	6.534730458705405E-5	0.0	24.163799553677908	0.0
38	4.574311321093783E-4	6.534730458705405E-5	0.0	24.685189360151867	0.0
39	4.7376795825614186E-4	6.534730458705405E-5	0.0	25.557183792561517	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	35920	0.0	43.590614	1
CGTTCTG	3510	0.0	39.294872	1
CGTTTCT	4625	0.0	38.432434	1
CGTAAGG	785	0.0	38.12102	2
CGTTTTC	2735	0.0	37.76051	1
GTTTTTT	41595	0.0	37.60548	2
TACGGGA	1990	0.0	35.05025	4
ACGATAG	340	0.0	34.411762	1
ACGGGTA	600	0.0	34.125004	5
CGGTCTA	395	0.0	33.607597	31
ACAACGA	1135	0.0	33.502205	13
TTAACGG	440	0.0	33.238636	2
GACCGAT	2420	0.0	33.099174	9
CACAACG	1190	0.0	32.710083	12
TGGGCGA	2445	0.0	32.668713	6
TACGGGT	670	0.0	32.574627	4
GGGCGAT	8455	0.0	32.465996	7
ACGGGAT	2180	0.0	31.995415	5
CTAAGCG	155	0.0	31.935486	1
AGGGCGA	3975	0.0	31.867926	6
>>END_MODULE