##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935052.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2235748
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.216258272399216	33.0	31.0	34.0	30.0	34.0
2	32.39486650552745	34.0	31.0	34.0	30.0	34.0
3	32.41298035377869	34.0	31.0	34.0	30.0	34.0
4	35.94473795794517	37.0	35.0	37.0	35.0	37.0
5	35.85408910127617	37.0	35.0	37.0	35.0	37.0
6	35.78269286162841	37.0	35.0	37.0	35.0	37.0
7	36.08606068304657	37.0	35.0	37.0	35.0	37.0
8	35.88757051331366	37.0	35.0	37.0	35.0	37.0
9	37.63960696822719	39.0	38.0	39.0	35.0	39.0
10	37.402794053712675	39.0	37.0	39.0	35.0	39.0
11	37.27210490627745	39.0	37.0	39.0	34.0	39.0
12	36.93507497267134	39.0	35.0	39.0	33.0	39.0
13	36.73672748449289	39.0	35.0	39.0	33.0	39.0
14	37.44695332389876	40.0	36.0	41.0	32.0	41.0
15	37.65187668735474	40.0	36.0	41.0	33.0	41.0
16	37.82358432166774	40.0	36.0	41.0	33.0	41.0
17	37.89730372117072	40.0	36.0	41.0	33.0	41.0
18	37.91264579013377	40.0	36.0	41.0	33.0	41.0
19	37.88100626725373	40.0	36.0	41.0	33.0	41.0
20	37.832420290658874	40.0	35.0	41.0	33.0	41.0
21	37.77385331441647	40.0	35.0	41.0	33.0	41.0
22	37.795964035302724	40.0	35.0	41.0	33.0	41.0
23	37.605918019383225	39.0	35.0	41.0	33.0	41.0
24	37.36051066578165	39.0	35.0	41.0	33.0	41.0
25	37.30685121936819	39.0	35.0	41.0	33.0	41.0
26	37.49905579698606	39.0	35.0	41.0	33.0	41.0
27	37.55351900124701	39.0	35.0	41.0	33.0	41.0
28	37.51571465120398	39.0	35.0	41.0	33.0	41.0
29	37.56386363758349	39.0	35.0	41.0	33.0	41.0
30	37.46967860420763	39.0	35.0	41.0	33.0	41.0
31	37.341195877173995	39.0	35.0	41.0	33.0	41.0
32	37.2834925939775	39.0	35.0	41.0	33.0	41.0
33	37.25399776719022	39.0	35.0	41.0	33.0	41.0
34	37.08493264893897	39.0	35.0	41.0	32.0	41.0
35	36.95602813912838	39.0	35.0	41.0	32.0	41.0
36	36.93345493320356	39.0	35.0	41.0	32.0	41.0
37	36.87912412311226	39.0	35.0	41.0	32.0	41.0
38	36.79225834038541	39.0	35.0	41.0	32.0	41.0
39	36.74121770432088	39.0	35.0	41.0	31.0	41.0
40	36.61961947410889	39.0	35.0	41.0	31.0	41.0
41	36.50144962670211	39.0	35.0	41.0	31.0	41.0
42	36.54854885255404	39.0	35.0	41.0	31.0	41.0
43	36.49101598212321	38.0	35.0	41.0	31.0	41.0
44	36.36157831741323	38.0	35.0	40.0	31.0	41.0
45	36.33707823958693	38.0	35.0	40.0	31.0	41.0
46	36.21759518514609	38.0	35.0	40.0	31.0	41.0
47	36.1286598489633	38.0	35.0	40.0	31.0	41.0
48	36.06347718973695	38.0	35.0	40.0	31.0	41.0
49	36.01576385174	37.0	35.0	40.0	31.0	41.0
50	35.93070149229698	37.0	35.0	40.0	31.0	41.0
51	34.854486283785114	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	26.0
10	45.0
11	44.0
12	23.0
13	51.0
14	58.0
15	109.0
16	206.0
17	347.0
18	646.0
19	1067.0
20	1776.0
21	2864.0
22	4271.0
23	6137.0
24	8405.0
25	11189.0
26	14059.0
27	17384.0
28	20894.0
29	26446.0
30	33957.0
31	44872.0
32	60809.0
33	93892.0
34	190778.0
35	197546.0
36	159307.0
37	258142.0
38	460131.0
39	619798.0
40	457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98017844587136	21.62256211344033	25.80026908220426	10.596990358484051
2	30.45906783769906	23.900681114329522	25.868210549668387	19.772040498303028
3	31.64345892291976	24.18868316107182	26.521996217820615	17.645861698187808
4	28.565652300706518	25.337605132599915	25.220910406718467	20.875832159975097
5	25.646808137589744	29.05662892240091	25.310813204350403	19.98574973565894
6	27.141207327480554	31.534681010561115	24.895515952602885	16.428595709355438
7	80.6956329604231	5.414429533203205	8.437042099556837	5.452895406816868
8	81.6252994523533	3.8108498811136142	8.467770070687752	6.0960805958453275
9	76.56636615575637	6.001705022211805	10.332872935590236	7.099055886441584
10	45.14703803827623	23.506719004109584	16.70143504545235	14.644807912161836
11	35.869561328020865	21.63021056040305	22.656041736367428	19.844186375208654
12	31.176098558513747	20.09443819249754	27.883553960464237	20.845909288524467
13	27.861324263736343	22.607422661230157	30.765810815888017	18.765442259145484
14	20.65872361285798	24.78888497272501	33.00524030436346	21.547151110053548
15	19.231416062991	22.531385469203148	36.38701678364467	21.850181684161186
16	26.7226896770119	24.074537917511275	28.249069215314073	20.953703190162756
17	26.51676306989875	22.990292286966152	25.295963588025128	25.19698105510997
18	28.531972297414555	21.719442441634744	29.1157142933819	20.632870967568795
19	25.798390516283586	24.771620057358877	26.191368615783173	23.23862081057436
20	24.891087904361314	28.42601223393692	26.504149841574275	20.17875002012749
21	22.981480918243022	26.56627670023634	30.453611050977123	19.998631330543514
22	22.50754557311468	23.05228496234817	30.102766501412503	24.337402963124646
23	20.873953594054427	25.916248163925452	30.224068186575586	22.98573005544453
24	23.5909413762195	24.979246319352626	28.02310457171381	23.406707732714064
25	23.713763805223127	25.898357059919096	25.28983588490295	25.098043249954827
26	22.893725053091853	23.984366753319247	26.294779196939906	26.827128996648998
27	22.67460375677402	22.806013915700696	28.5582722203039	25.961110107221387
28	18.23886234047844	24.909068463887703	27.817446331160756	29.034622864473096
29	22.73713316527623	25.781796517317694	25.807470251566812	25.673600065839263
30	25.360282106927972	22.51134743271603	27.123495134514265	25.00487532584173
31	25.26635381089461	23.20816120600354	27.39848140309194	24.127003580009912
32	26.086034740945756	23.430413445522483	24.987655138235613	25.49589667529614
33	24.426724299876373	22.171595367635348	25.604137854534592	27.797542477953684
34	21.063778207561853	22.245932904781757	32.24571821153368	24.444570676122712
35	21.40956852024468	25.676731009040378	27.715690677124616	25.19800979359033
36	23.36126432853792	25.613351773097865	26.868367991383646	24.157015906980572
37	20.070151018808918	26.469754194122057	28.94843247092248	24.51166231614654
38	21.093924717812563	26.934833442767253	25.631600699184343	26.33964114023584
39	22.12158973193759	21.840028482637578	27.52452423081671	28.513857554608123
40	25.400134541102126	21.55408391285601	28.428785355057904	24.616996190983954
41	20.589171946033275	23.166966939028907	26.489121314208937	29.754739800728885
42	21.956231203158854	22.102267339610727	30.319696137489554	25.621805319740865
43	22.22567122949456	21.08055111756781	29.896750438779325	26.7970272141583
44	22.661901072929506	20.38543699916091	27.97376985241628	28.978892075493302
45	22.15951887243106	19.95613995852842	26.28909877141789	31.59524239762263
46	22.80793720938138	21.15301008879355	27.93816655544364	28.100886146381438
47	18.489114157767332	23.96204760107132	31.805954875057473	25.742883366103875
48	19.358353445916087	24.309984846234904	29.549081560175832	26.782580147673173
49	23.91812494073572	20.197580407094183	31.024292541019825	24.860002111150273
50	20.358376704351294	21.42880145705151	29.68612741686451	28.526694421732685
51	19.39690877504978	21.758266137328537	27.008768430073516	31.836056657548166
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1047.0
1	1568.0
2	2089.0
3	3049.5
4	4010.0
5	2983.5
6	1957.0
7	2057.5
8	2158.0
9	2280.5
10	2403.0
11	2586.5
12	2770.0
13	2897.5
14	3025.0
15	3079.5
16	3134.0
17	3080.5
18	3027.0
19	3095.5
20	3164.0
21	3456.0
22	3748.0
23	4612.5
24	5477.0
25	6136.5
26	8625.5
27	10455.0
28	12832.0
29	15209.0
30	17834.5
31	20460.0
32	22812.0
33	25164.0
34	31841.5
35	38519.0
36	41061.5
37	43604.0
38	47933.0
39	52262.0
40	61292.0
41	70322.0
42	83103.5
43	95885.0
44	110915.0
45	125945.0
46	151739.0
47	177533.0
48	212375.0
49	247217.0
50	253886.0
51	260555.0
52	235677.0
53	210799.0
54	192789.0
55	174779.0
56	160785.5
57	146792.0
58	140017.0
59	133242.0
60	114096.5
61	94951.0
62	84664.5
63	74378.0
64	66062.0
65	57746.0
66	48098.5
67	38451.0
68	35486.0
69	32521.0
70	24801.0
71	17081.0
72	14384.0
73	11687.0
74	9274.0
75	5286.0
76	3711.0
77	3141.0
78	2571.0
79	1947.0
80	1323.0
81	947.0
82	571.0
83	353.0
84	135.0
85	122.0
86	109.0
87	96.0
88	83.0
89	47.5
90	12.0
91	9.5
92	7.0
93	3.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2235748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.643404303516427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.34677267319452	12.639896712460224
2	12.995501753972949	5.10551790160695
3	5.968252993065045	3.5171041958534612
4	3.497618748834605	2.7482055673166967
5	2.262717992998238	2.222374218065282
6	1.5563142913938333	1.8342786509513844
7	1.0835570461628294	1.489932440059033
8	0.8656069996752844	1.360277461006034
9	0.6632438514557376	1.17255304133698
>10	4.54538966686138	18.005355782484322
>50	1.2236289564231926	17.767164664203964
>100	0.9703654474829706	26.955525046979574
>500	0.014096750409269962	1.8929169972209752
>1k	0.006008450994115065	1.9405361231013274
>5k	6.932828070132767E-4	0.8660416430365661
>10k+	2.3109426900442555E-4	0.48231955431719853
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	10625	0.47523245016880256	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	7737	0.34605867924292	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	5720	0.2558427872908754	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	5621	0.2514147390493025	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	4066	0.18186307222459774	No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	3016	0.1348989242079161	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2909	0.13011305388621616	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	2359	0.10551278587747814	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.47277600158873E-5	0.0	0.0	0.2680534657752126	0.0
2	4.47277600158873E-5	0.0	0.0	1.4654603291605315	0.0
3	8.94555200317746E-5	0.0	0.0	2.106632768988276	0.0
4	8.94555200317746E-5	0.0	0.0	2.9009977868704344	0.0
5	8.94555200317746E-5	0.0	0.0	5.036748327629053	0.0
6	8.94555200317746E-5	0.0	0.0	6.118220837053192	0.0
7	1.341832800476619E-4	0.0	0.0	7.293979464590821	0.0
8	1.341832800476619E-4	0.0	0.0	9.007991956159639	0.0
9	1.341832800476619E-4	0.0	0.0	9.59817474956927	0.0
10	1.341832800476619E-4	0.0	0.0	10.965860195335074	0.0
11	1.341832800476619E-4	0.0	0.0	13.16579507171649	0.0
12	1.341832800476619E-4	0.0	0.0	14.794780091495106	0.0
13	1.789110400635492E-4	0.0	0.0	15.487478910861153	0.0
14	2.236388000794365E-4	0.0	0.0	15.753027622075475	0.0
15	2.236388000794365E-4	0.0	0.0	16.25889858785516	0.0
16	2.236388000794365E-4	0.0	0.0	17.201983407790145	0.0
17	2.236388000794365E-4	0.0	0.0	18.310739850823975	0.0
18	2.236388000794365E-4	0.0	0.0	19.566605896550058	0.0
19	2.236388000794365E-4	0.0	0.0	20.35766106019104	0.0
20	2.236388000794365E-4	0.0	0.0	21.154128282793945	0.0
21	2.236388000794365E-4	0.0	0.0	22.072031373839984	0.0
22	2.236388000794365E-4	0.0	0.0	23.00129531593006	0.0
23	2.236388000794365E-4	0.0	0.0	23.86794039399789	0.0
24	2.683665600953238E-4	0.0	0.0	24.56269669032467	0.0
25	2.683665600953238E-4	0.0	0.0	25.240814259925536	0.0
26	2.683665600953238E-4	0.0	0.0	25.86297740174653	0.0
27	2.683665600953238E-4	0.0	0.0	26.66677997699204	0.0
28	2.683665600953238E-4	0.0	0.0	27.285991086651983	0.0
29	2.683665600953238E-4	0.0	0.0	27.97023635937503	0.0
30	2.683665600953238E-4	0.0	0.0	28.79479261526791	0.0
31	2.683665600953238E-4	0.0	0.0	29.388016896358625	0.0
32	2.683665600953238E-4	0.0	0.0	29.986921602971353	0.0
33	3.130943201112111E-4	0.0	0.0	30.589359802625342	0.0
34	4.47277600158873E-4	0.0	0.0	31.27888295103026	0.0
35	4.47277600158873E-4	0.0	0.0	31.974310163757274	0.0
36	4.47277600158873E-4	0.0	0.0	32.54123452195865	0.0
37	4.47277600158873E-4	0.0	0.0	33.166707518020814	0.0
38	4.920053601747604E-4	0.0	0.0	33.927750354691135	0.0
39	4.920053601747604E-4	0.0	0.0	35.23156455915425	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCGA	20	7.034718E-4	45.000004	1
AACGGTA	20	7.034718E-4	45.000004	25
AATGCGC	20	7.034718E-4	45.000004	28
TCCGTCG	20	7.034718E-4	45.000004	38
ATGCGAA	60	0.0	45.000004	26
CGTCGCA	20	7.034718E-4	45.000004	14
CGTTTCG	20	7.034718E-4	45.000004	28
CGTCAAT	20	7.034718E-4	45.000004	32
TGTCGAT	30	2.1664255E-6	45.000004	33
CCGTTAT	20	7.034718E-4	45.000004	39
ACGTTAT	25	3.8919934E-5	45.0	41
TTTAACG	25	3.8919934E-5	45.0	1
CGTAGTC	25	3.8919934E-5	45.0	29
AGTCGTA	25	3.8919934E-5	45.0	28
CGACGGT	145	0.0	43.448273	28
TCACGAC	155	0.0	42.09677	25
CGGTCTA	150	0.0	42.000004	31
CAATGCG	65	0.0	41.53846	1
AATCCGT	60	3.6379788E-12	41.250004	39
ATTAGCG	110	0.0	40.909092	1
>>END_MODULE