##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935051.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2969689
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34962718318315	33.0	31.0	34.0	30.0	34.0
2	32.529606298841394	34.0	31.0	34.0	31.0	34.0
3	32.53166880437649	34.0	31.0	34.0	31.0	34.0
4	36.03845520524203	37.0	35.0	37.0	35.0	37.0
5	35.94866937244944	37.0	35.0	37.0	35.0	37.0
6	35.87129999134589	37.0	35.0	37.0	35.0	37.0
7	36.1036886354093	37.0	35.0	37.0	35.0	37.0
8	35.72873051689925	37.0	35.0	37.0	35.0	37.0
9	37.5497201895552	39.0	38.0	39.0	35.0	39.0
10	37.446433953185	39.0	37.0	39.0	35.0	39.0
11	37.38098164487931	39.0	37.0	39.0	35.0	39.0
12	37.1234708415595	39.0	37.0	39.0	34.0	39.0
13	36.964874436346705	39.0	37.0	39.0	33.0	39.0
14	37.87948670719392	40.0	37.0	41.0	33.0	41.0
15	37.985025031240646	40.0	37.0	41.0	33.0	41.0
16	38.198271603524816	40.0	37.0	41.0	33.0	41.0
17	38.20829554879316	40.0	37.0	41.0	34.0	41.0
18	38.231443763976635	40.0	37.0	41.0	34.0	41.0
19	38.20891749944186	40.0	37.0	41.0	34.0	41.0
20	38.07603658160838	40.0	36.0	41.0	33.0	41.0
21	38.078622037526486	40.0	36.0	41.0	34.0	41.0
22	38.08052324671034	40.0	36.0	41.0	34.0	41.0
23	37.86313246942694	40.0	36.0	41.0	34.0	41.0
24	37.665156519756785	40.0	35.0	41.0	33.0	41.0
25	37.613403962502474	40.0	35.0	41.0	33.0	41.0
26	37.75076784134635	40.0	35.0	41.0	33.0	41.0
27	37.7648033177885	40.0	36.0	41.0	33.0	41.0
28	37.72194125378112	40.0	36.0	41.0	33.0	41.0
29	37.71622786089722	40.0	36.0	41.0	33.0	41.0
30	37.62868098309284	40.0	36.0	41.0	33.0	41.0
31	37.52510347043074	40.0	35.0	41.0	33.0	41.0
32	37.42884490598174	40.0	35.0	41.0	33.0	41.0
33	37.32011769582606	40.0	35.0	41.0	33.0	41.0
34	37.135827354312184	39.0	35.0	41.0	32.0	41.0
35	37.069125083468336	39.0	35.0	41.0	32.0	41.0
36	37.01273163620837	39.0	35.0	41.0	32.0	41.0
37	36.982692800491904	39.0	35.0	41.0	32.0	41.0
38	36.81954204632202	39.0	35.0	41.0	31.0	41.0
39	36.77672577835592	39.0	35.0	41.0	31.0	41.0
40	36.63387849704127	39.0	35.0	41.0	31.0	41.0
41	36.60821789756436	39.0	35.0	41.0	31.0	41.0
42	36.63811698800784	39.0	35.0	41.0	31.0	41.0
43	36.597943757747025	39.0	35.0	41.0	31.0	41.0
44	36.53849679208833	39.0	35.0	41.0	31.0	41.0
45	36.53906587524822	39.0	35.0	41.0	31.0	41.0
46	36.46126883993576	39.0	35.0	41.0	31.0	41.0
47	36.33885063385425	38.0	35.0	40.0	31.0	41.0
48	36.294486055610534	38.0	35.0	40.0	31.0	41.0
49	36.27737517295582	38.0	35.0	40.0	31.0	41.0
50	36.165651352717404	38.0	35.0	40.0	31.0	41.0
51	35.135941170944164	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	45.0
10	45.0
11	46.0
12	54.0
13	61.0
14	69.0
15	127.0
16	231.0
17	442.0
18	753.0
19	1503.0
20	2797.0
21	4793.0
22	7620.0
23	10579.0
24	13240.0
25	16565.0
26	19484.0
27	22253.0
28	26232.0
29	31939.0
30	40261.0
31	53698.0
32	73970.0
33	111333.0
34	214004.0
35	231831.0
36	207424.0
37	332414.0
38	593351.0
39	951583.0
40	923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.52395890613462	20.240099215776468	23.791009765669067	10.444932112419853
2	30.272294506259744	28.172377646278786	24.182801633437037	17.372526214024433
3	30.489657334488562	27.74866324386156	24.958539429549695	16.80313999210018
4	27.384045938817163	24.69342749358603	29.288689825769637	18.633836741827174
5	29.38045027610635	27.954509714653618	23.37638722438612	19.288652784853902
6	26.33491251104072	35.70215601701053	23.424473067718537	14.538458404230207
7	78.17007100743545	5.731778647528412	11.271045553928374	4.827104791107756
8	78.5092984484234	7.866918051014769	8.016866412610883	5.60691708795096
9	73.04205928634278	7.010397385046044	9.191703238958693	10.755840089652486
10	43.273117151324605	25.039692708563084	16.85509829480461	14.832091845307707
11	34.74616365552083	22.691399671817486	23.159462152434145	19.402974520227538
12	29.545215003995366	21.029070720873467	28.200360374436517	21.225353900694653
13	27.358150971364342	22.30805986754842	31.672070711781604	18.661718449305635
14	21.30751738650074	26.796644362423134	31.26677574655124	20.629062504524885
15	19.23440468008603	24.076830940883035	36.18180220218346	20.506962176847473
16	24.860684064897033	24.65672331345134	27.521063653466744	22.961528968184883
17	25.13118377042175	24.399120581313397	27.73532851419795	22.7343671340669
18	25.518598075421366	22.819628587370598	29.402641151985947	22.25913218522209
19	24.97604968062312	25.373768094908257	26.95686315974501	22.693319064723614
20	24.42501554876622	28.09479376459959	28.658691196283513	18.821499490350675
21	23.00655051757945	28.160053123407874	30.382070311066244	18.451326047946434
22	21.71261031037257	22.366517167285867	31.937283668424538	23.983588853917027
23	21.49814340828282	26.03808008178634	30.572460617930027	21.89131589200081
24	22.812018362865608	25.641708609891474	27.66623036957742	23.8800426576655
25	22.489526681076704	28.644716668984533	25.54998183311451	23.315774816824252
26	20.322936172777688	25.86530104667526	27.622454741893847	26.18930803865321
27	21.790429906970058	26.051818894167035	27.782370477177913	24.375380721684998
28	18.360205395245092	26.76546264608853	28.917135767415374	25.957196191251004
29	22.28748532253714	27.076269602641894	27.007811255656737	23.62843381916423
30	22.5099328582892	26.562579448555052	27.491430920880937	23.436056772274807
31	23.834111922157504	26.741386050862566	26.035857626842407	23.388644400137522
32	27.379432661130508	27.00673370174453	24.05790640029983	21.555927236825134
33	24.825730909869687	24.208427212411802	25.80239883704994	25.16344304066857
34	21.75103184205484	26.846346536623866	27.603530201310644	23.799091420010647
35	21.81730140765582	28.699436203588995	25.393500800925615	24.089761587829567
36	24.67143865906497	28.944916454214564	24.299514191553392	22.08413069516707
37	22.472790921877678	27.68050795891422	26.092967984189592	23.753733135018514
38	23.468215021842354	28.27120954416439	25.261971876516366	22.998603557476894
39	24.707469368004528	24.039251248194677	25.990566688969786	25.26271269483101
40	22.854750110196726	24.277390662793312	29.296670459431944	23.57118876757802
41	21.552728248648258	25.773035492942192	24.963253727915617	27.710982530493933
42	21.501174028660913	25.753942584560203	29.028056473253592	23.71682691352529
43	23.66924617358922	24.516708651983425	27.339462145699432	24.474583028727924
44	22.880240994932464	23.541252972954407	27.835136945316496	25.74336908679663
45	21.33529807329993	23.837580298812437	26.31659409453313	28.5105275333545
46	23.888999824560752	24.886646379469365	26.660199098289418	24.564154697680465
47	19.31576000045796	25.25823411138338	31.733962714614222	23.692043173544437
48	21.372844092428533	27.00087450234688	27.155133079591838	24.471148325632754
49	23.64779611602427	23.477811986372984	29.896261864457863	22.978130033144886
50	21.792820729712776	24.711476521615563	27.25090068353959	26.244802065132074
51	20.486657020314247	25.112225556278787	25.583756413550375	28.81736100985659
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1201.0
1	2270.0
2	3339.0
3	5083.5
4	6828.0
5	5076.0
6	3324.0
7	3396.0
8	3468.0
9	3779.0
10	4090.0
11	4184.5
12	4279.0
13	4440.0
14	4601.0
15	4586.5
16	4572.0
17	4590.5
18	4609.0
19	4889.0
20	5169.0
21	6391.0
22	7613.0
23	9653.5
24	11694.0
25	14235.5
26	20068.5
27	23360.0
28	27203.0
29	31046.0
30	35300.0
31	39554.0
32	45096.5
33	50639.0
34	60650.0
35	70661.0
36	77309.5
37	83958.0
38	91380.5
39	98803.0
40	110889.0
41	122975.0
42	133921.0
43	144867.0
44	161998.0
45	179129.0
46	256132.5
47	333136.0
48	316059.5
49	298983.0
50	293751.0
51	288519.0
52	252427.0
53	216335.0
54	201057.0
55	185779.0
56	171757.5
57	157736.0
58	149736.0
59	141736.0
60	126289.5
61	110843.0
62	98528.5
63	86214.0
64	77945.0
65	69676.0
66	60447.5
67	51219.0
68	45295.5
69	39372.0
70	31056.5
71	22741.0
72	19279.5
73	15818.0
74	13596.5
75	9122.5
76	6870.0
77	5375.5
78	3881.0
79	2707.0
80	1533.0
81	1176.0
82	819.0
83	604.5
84	390.0
85	246.0
86	102.0
87	61.5
88	21.0
89	17.5
90	14.0
91	11.0
92	8.0
93	7.5
94	7.0
95	5.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2969689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.84768646223888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.10290573307452	12.835822144243995
2	12.27820973130338	4.628316946664327
3	5.3855544057397955	3.045157225941387
4	2.9581888201608475	2.23020061513968
5	1.767225522525497	1.6654056278313418
6	1.1645550509685254	1.3169501081202832
7	0.8315710502020704	1.0971233297698333
8	0.6477873351741691	0.9767434070057588
9	0.49286904800940257	0.8360497155441043
>10	3.850860079187588	15.365119084143513
>50	1.332680125303759	18.630638957300803
>100	1.173394622988939	30.638415932918928
>500	0.008373459828542516	1.0319735154057401
>1k	0.004368761649674356	1.7865949280640485
>5k	7.281269416123926E-4	0.8020018250648153
>10k+	7.281269416123926E-4	3.1134866368415013
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	29312	0.9870393835852845	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	26125	0.8797217486410193	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	25278	0.8512002435271843	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	10034	0.33788049859766456	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGTCAGT	6445	0.21702609263124858	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	6388	0.21510669972512275	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCT	5324	0.17927803214410667	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	5219	0.1757423083696643	No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT	4795	0.16146471903286844	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTC	4185	0.14092384758134607	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCT	4043	0.13614220209590971	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTA	4010	0.1350309746239421	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	3267	0.11001151972479273	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3006	0.10122272062832169	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.683677987829702E-4	0.0	0.0	0.169546373374451	0.0
2	1.683677987829702E-4	0.0	0.0	0.9176381769269442	0.0
3	1.683677987829702E-4	0.0	0.0	1.280302415505462	0.0
4	1.683677987829702E-4	0.0	0.0	1.852315175090725	0.0
5	1.683677987829702E-4	0.0	0.0	3.894077797372048	0.0
6	1.683677987829702E-4	0.0	0.0	4.600246019027582	0.0
7	1.683677987829702E-4	0.0	0.0	5.47582591981854	0.0
8	1.683677987829702E-4	0.0	0.0	6.623454509883022	0.0
9	1.683677987829702E-4	0.0	0.0	7.000430011358092	0.0
10	1.683677987829702E-4	0.0	0.0	8.827591037310642	0.0
11	1.683677987829702E-4	0.0	0.0	10.208880458526128	0.0
12	1.683677987829702E-4	0.0	0.0	12.405171046530462	0.0
13	1.683677987829702E-4	0.0	0.0	12.900340742751178	0.0
14	2.0204135853956425E-4	0.0	0.0	13.137537297676625	0.0
15	2.0204135853956425E-4	0.0	0.0	13.632774341016853	0.0
16	2.0204135853956425E-4	0.0	0.0	14.330995602569832	0.0
17	2.3571491829615828E-4	0.0	0.0	15.11481505302407	0.0
18	6.061240756186927E-4	0.0	0.0	15.942006048444803	0.0
19	6.061240756186927E-4	0.0	0.0	16.761283757322737	0.0
20	6.061240756186927E-4	0.0	0.0	17.368923143130477	0.0
21	6.061240756186927E-4	0.0	0.0	18.032090228976838	0.0
22	6.061240756186927E-4	0.0	0.0	18.75418604439724	0.0
23	6.061240756186927E-4	0.0	0.0	19.39455613028839	0.0
24	6.061240756186927E-4	0.0	0.0	19.936801463048823	0.0
25	6.061240756186927E-4	0.0	0.0	20.42941196872804	0.0
26	6.061240756186927E-4	0.0	0.0	20.910809179008307	0.0
27	6.397976353752868E-4	0.0	0.0	21.517000601746513	0.0
28	6.397976353752868E-4	0.0	0.0	22.012406012885524	0.0
29	6.734711951318808E-4	0.0	0.0	22.551587051708108	0.0
30	6.734711951318808E-4	0.0	0.0	23.175356072639257	0.0
31	7.071447548884748E-4	0.0	0.0	23.652880823547516	0.0
32	7.071447548884748E-4	0.0	0.0	24.12026983296904	0.0
33	7.071447548884748E-4	0.0	0.0	24.599814997462698	0.0
34	7.408183146450689E-4	0.0	0.0	25.119768433664266	0.0
35	8.08165434158257E-4	0.0	0.0	25.666425002752813	0.0
36	8.75512553671445E-4	0.0	0.0	26.147283436076975	0.0
37	8.75512553671445E-4	0.0	0.0	26.670604228254206	0.0
38	8.75512553671445E-4	0.0	0.0	27.277031365910705	0.0
39	8.75512553671445E-4	0.0	0.0	28.36573122640115	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	40	6.8212103E-9	45.0	1
TATTACG	20	7.035094E-4	45.0	15
TTAGTCG	20	7.035094E-4	45.0	1
CACGTAT	45	3.8562575E-10	45.0	11
TACGGCT	3350	0.0	40.164177	7
TACGATA	40	3.460682E-7	39.375	24
TACGTAG	120	0.0	39.374996	1
ATGCGAT	35	6.251632E-6	38.57143	31
ATCCGGC	790	0.0	38.449368	6
TGCGTAG	195	0.0	38.076923	1
CGTTAGG	245	0.0	37.65306	2
GCGAACC	60	1.5643309E-10	37.499996	33
CTATACG	30	1.1403575E-4	37.499996	43
ACTCGAA	30	1.1403575E-4	37.499996	37
AACTCGA	30	1.1403575E-4	37.499996	36
CGTTACG	85	0.0	37.058823	45
TATAACG	85	0.0	37.058823	1
ACGGCTG	3670	0.0	36.907356	8
CCGATGA	1640	0.0	36.493904	18
TGATCCG	840	0.0	36.42857	4
>>END_MODULE