##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935050.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	572344
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23546678221489	33.0	31.0	34.0	30.0	34.0
2	32.39709510364396	34.0	31.0	34.0	30.0	34.0
3	32.40451721342409	34.0	31.0	34.0	30.0	34.0
4	35.955048013083044	37.0	35.0	37.0	35.0	37.0
5	35.874564946954976	37.0	35.0	37.0	35.0	37.0
6	35.8144245418839	37.0	35.0	37.0	35.0	37.0
7	36.09113225612569	37.0	35.0	37.0	35.0	37.0
8	35.83418014341026	37.0	35.0	37.0	35.0	37.0
9	37.56984610653733	39.0	38.0	39.0	35.0	39.0
10	37.43405714046098	39.0	37.0	39.0	35.0	39.0
11	37.303595390184924	39.0	37.0	39.0	34.0	39.0
12	36.96617943055225	39.0	35.0	39.0	34.0	39.0
13	36.65924688648785	39.0	35.0	39.0	33.0	39.0
14	37.67207134171058	40.0	36.0	41.0	33.0	41.0
15	37.864874970297585	40.0	36.0	41.0	33.0	41.0
16	37.989563968522425	40.0	36.0	41.0	34.0	41.0
17	38.04637770291992	40.0	36.0	41.0	34.0	41.0
18	38.03627014522735	40.0	36.0	41.0	34.0	41.0
19	37.984685783375035	40.0	36.0	41.0	34.0	41.0
20	37.923198286345276	40.0	35.0	41.0	34.0	41.0
21	37.852396460869684	40.0	35.0	41.0	34.0	41.0
22	37.7387794752806	39.0	35.0	41.0	34.0	41.0
23	37.532994143382304	39.0	35.0	41.0	33.0	41.0
24	37.31208329256531	39.0	35.0	41.0	33.0	41.0
25	37.274331520903516	39.0	35.0	41.0	33.0	41.0
26	37.4188215478803	39.0	35.0	41.0	33.0	41.0
27	37.46022147519673	39.0	35.0	41.0	33.0	41.0
28	37.41753386075507	39.0	35.0	41.0	33.0	41.0
29	37.42712774135834	39.0	35.0	41.0	33.0	41.0
30	37.34718281313336	39.0	35.0	41.0	33.0	41.0
31	37.19755950966552	39.0	35.0	41.0	33.0	41.0
32	37.12278629635324	39.0	35.0	41.0	33.0	41.0
33	36.97761835539466	39.0	35.0	41.0	33.0	41.0
34	36.89054834155682	39.0	35.0	41.0	32.0	41.0
35	36.824649162042405	39.0	35.0	41.0	32.0	41.0
36	36.73617439861342	39.0	35.0	41.0	32.0	41.0
37	36.691585829501136	39.0	35.0	41.0	32.0	41.0
38	36.587660567770435	39.0	35.0	41.0	31.0	41.0
39	36.52036013306682	39.0	35.0	41.0	31.0	41.0
40	36.46943097158352	39.0	35.0	41.0	31.0	41.0
41	36.492142837174846	39.0	35.0	41.0	31.0	41.0
42	36.48136085990244	39.0	35.0	41.0	31.0	41.0
43	36.379221237577404	38.0	35.0	40.0	31.0	41.0
44	36.216392589072306	38.0	35.0	40.0	31.0	41.0
45	36.19151244705981	38.0	35.0	40.0	31.0	41.0
46	36.07191304530143	38.0	35.0	40.0	31.0	41.0
47	36.01016172092308	38.0	35.0	40.0	31.0	41.0
48	35.98714409515956	38.0	35.0	40.0	31.0	41.0
49	35.959569769229695	38.0	35.0	40.0	31.0	41.0
50	35.868262793005606	38.0	35.0	40.0	31.0	41.0
51	34.8517936765302	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	9.0
11	6.0
12	13.0
13	5.0
14	12.0
15	13.0
16	33.0
17	84.0
18	160.0
19	307.0
20	531.0
21	881.0
22	1333.0
23	2139.0
24	2753.0
25	3320.0
26	3863.0
27	4428.0
28	5163.0
29	6207.0
30	7942.0
31	10482.0
32	14016.0
33	20491.0
34	43355.0
35	71440.0
36	39716.0
37	62497.0
38	115580.0
39	155451.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.059300001397766	23.38506213046699	25.754441384901387	10.80119648323386
2	29.686866639643288	25.653278447926418	26.55361111499378	18.106243797436505
3	30.46576883832101	25.41758103518164	26.205044518681074	17.911605607816277
4	27.856324168681773	27.027976182156188	26.277728079616452	18.83797156954559
5	25.319563059977916	29.22892526173071	24.938149085165566	20.513362593125812
6	26.60829850579372	33.64270438757111	23.577953119103196	16.171043987531974
7	77.7172469703535	6.93429126539284	9.465985491243028	5.882476273010637
8	77.21317948646269	5.238807430496345	9.573263631662076	7.974749451378892
9	72.59637560627874	7.801776553960556	11.527333212194064	8.074514627566638
10	42.28907789720867	25.78222188054736	17.953887871629647	13.974812350614316
11	36.17177781194527	22.495037949205372	22.104363809177695	19.228820429671668
12	32.7088254616105	20.793613630963197	28.544197196091858	17.95336371133444
13	22.37290860042212	31.549033448415635	29.24569839117733	16.832359559984905
14	16.237088184728066	36.00317291698698	27.766343318004555	19.99339558028039
15	14.534790265993877	25.980354472135637	40.55166123869561	18.933194023174874
16	17.219539298044534	25.014501768167396	35.19334526089205	22.572613672896022
17	17.449995107837243	24.154704163929384	26.81149798023566	31.58380274799771
18	19.28839998322687	25.510357407433293	32.09049103336455	23.11075157597529
19	22.69928574423773	25.856303202269963	27.40921543687013	24.035195616622172
20	24.483003228827418	27.494304124792084	26.742832981563534	21.279859664816964
21	22.694568301580865	27.497274366464925	28.697776162587534	21.110381169366672
22	19.187935926645512	27.81631326614763	25.787638203597833	27.20811260360902
23	18.10711739792852	28.96876703520959	27.26716799686902	25.656947569992873
24	21.617943055225528	24.618411305089246	30.335252924814448	23.42839271487078
25	17.159959744489328	28.148805613407323	29.339697804117804	25.35153683798555
26	16.56678501041332	32.5234474372056	27.410263757460545	23.499503794920535
27	19.615301287337687	31.240827194833876	29.173713710635564	19.970157807192876
28	15.11783123436255	30.475203723634735	31.194526368757252	23.21243867324546
29	16.220489775379843	27.64421396922131	30.84316425087011	25.292132004528746
30	20.44277567337126	28.588576101086062	27.23781502033742	23.73083320520526
31	22.516703241407264	31.254630082607665	22.259689976657395	23.968976699327676
32	22.404882378429754	29.527172469703533	21.87058831751534	26.19735683435137
33	21.88683728666676	29.244475350488518	22.916637546650268	25.952049816194457
34	20.317151922619963	27.840249919628757	25.544777266818556	26.297820890932726
35	17.979397005996393	26.02857721929469	25.73993961673399	30.252086157974922
36	22.669233887312526	28.407391359042812	26.130963196958472	22.792411556686186
37	20.515983394601847	28.385201906545714	28.32562235299051	22.77319234586193
38	18.285157178200524	28.625966202144166	23.334917462225516	29.753959157429797
39	21.397096850844946	26.42833680443929	25.632137316019737	26.54242902869603
40	24.335190025579024	23.332995541143088	26.475336510909525	25.856477922368367
41	18.800057308192276	26.51132885118041	24.658596927721792	30.030016912905527
42	21.28614958835945	25.845295836070616	26.189843870120065	26.67871070544987
43	21.406881176355476	26.181107865199948	27.16705338048446	25.244957577960108
44	18.999238220370966	27.132633521099198	26.223739569210125	27.64438868931971
45	20.631473379645808	25.6964343122318	24.53856422011937	29.13352808800302
46	23.121409501977833	25.023063052989116	26.08483709098025	25.770690354052807
47	18.373390827893715	25.308206253581762	30.289650979131434	26.02875193939309
48	18.956257076163986	22.55846134492543	29.85529681450317	28.629984764407418
49	20.067476902002994	21.408628377339504	32.13452049816195	26.389374222495558
50	18.75515424290287	23.877598087863245	29.610863396838266	27.756384272395625
51	18.248116517339223	23.73502648756692	25.223991166151826	32.792865828942034
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	998.0
1	1045.0
2	1092.0
3	1140.5
4	1189.0
5	1005.0
6	821.0
7	863.5
8	906.0
9	998.0
10	1090.0
11	1222.0
12	1354.0
13	1448.0
14	1542.0
15	1536.0
16	1530.0
17	1689.0
18	1848.0
19	1953.5
20	2059.0
21	1914.5
22	1770.0
23	1616.5
24	1463.0
25	2239.0
26	2980.0
27	2945.0
28	3416.0
29	3887.0
30	5551.0
31	7215.0
32	6755.5
33	6296.0
34	7406.5
35	8517.0
36	9360.0
37	10203.0
38	13346.0
39	16489.0
40	19991.0
41	23493.0
42	31568.5
43	39644.0
44	44620.0
45	49596.0
46	58331.0
47	67066.0
48	70077.0
49	73088.0
50	73550.0
51	74012.0
52	61782.0
53	49552.0
54	40295.0
55	31038.0
56	27006.0
57	22974.0
58	19926.0
59	16878.0
60	15084.0
61	13290.0
62	12135.5
63	10981.0
64	9742.0
65	8503.0
66	7238.5
67	5974.0
68	4962.0
69	3950.0
70	3015.5
71	2081.0
72	1717.0
73	1353.0
74	1218.0
75	984.0
76	885.0
77	652.0
78	419.0
79	276.5
80	134.0
81	81.5
82	29.0
83	54.5
84	80.0
85	42.5
86	5.0
87	5.5
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	572344.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.15451909572962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.4913056058806	15.62728724447086
2	12.080327207355491	5.594283340107496
3	5.758503448854569	4.00006134207984
4	3.3248466887931225	3.0794090458413503
5	2.1422043971976783	2.48008563109348
6	1.4780529558542626	2.053416323449628
7	1.0686369708592707	1.732064260371455
8	0.7847372037764575	1.4536170055977162
9	0.6330312149053237	1.3191780018346415
>10	3.443975312650186	15.437725899677583
>50	0.9286498806482677	15.95410939434493
>100	0.8543578901964062	27.940360113362633
>500	0.007580815352230755	1.1995729346244761
>1k	0.0037904076761153776	2.1288294631439344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	4151	0.725263128468194	No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	3090	0.5398851040632906	No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT	2200	0.3843842164851907	No Hit
TCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1437	0.2510727814041905	No Hit
ACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1250	0.21840012300294928	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	833	0.1455418419691654	No Hit
ACCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	754	0.131738954195379	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	754	0.131738954195379	No Hit
GCCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	735	0.1284192723257342	No Hit
GTGGAAGGGGATGGAGCTGGGTAAGGAGTGAAGGGATTTCTGGAAGCAAAC	703	0.12282822917685868	No Hit
AAAACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTC	692	0.12090630809443272	No Hit
CGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	609	0.1064045399270369	No Hit
ACCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATAC	594	0.1037837384510015	No Hit
AGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	584	0.1020365374669779	No Hit
GGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	576	0.10063877667975904	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4944019680471886E-4	0.0	0.0	0.4437890499419929	0.0
2	3.4944019680471886E-4	0.0	0.0	2.4609325859972326	0.0
3	3.4944019680471886E-4	0.0	0.0	3.4170009644549433	0.0
4	3.4944019680471886E-4	0.0	0.0	4.507429098584068	0.0
5	3.4944019680471886E-4	0.0	0.0	7.440455390464476	0.0
6	3.4944019680471886E-4	0.0	0.0	8.867394434116544	0.0
7	3.4944019680471886E-4	0.0	0.0	10.391128412283521	0.0
8	3.4944019680471886E-4	0.0	0.0	12.63925191842668	0.0
9	3.4944019680471886E-4	0.0	0.0	13.403128188641796	0.0
10	3.4944019680471886E-4	0.0	0.0	15.148581971681367	0.0
11	3.4944019680471886E-4	0.0	0.0	17.861286219476398	0.0
12	3.4944019680471886E-4	0.0	0.0	19.82461596522371	0.0
13	3.4944019680471886E-4	0.0	0.0	20.587618634946814	0.0
14	3.4944019680471886E-4	0.0	0.0	20.867170792390592	0.0
15	3.4944019680471886E-4	0.0	0.0	21.416316061669207	0.0
16	3.4944019680471886E-4	0.0	0.0	22.672728289280574	0.0
17	3.4944019680471886E-4	0.0	0.0	24.087436926044475	0.0
18	5.241602952070783E-4	0.0	0.0	25.927589002418127	0.0
19	5.241602952070783E-4	0.0	0.0	26.83001831066631	0.0
20	5.241602952070783E-4	0.0	0.0	27.679682149196985	0.0
21	5.241602952070783E-4	0.0	0.0	28.671917588023984	0.0
22	5.241602952070783E-4	0.0	0.0	29.603175712508563	0.0
23	5.241602952070783E-4	0.0	0.0	30.5761919405113	0.0
24	5.241602952070783E-4	0.0	0.0	31.29673762632263	0.0
25	5.241602952070783E-4	0.0	0.0	31.930622143326392	0.0
26	5.241602952070783E-4	0.0	0.0	32.54860713137553	0.0
27	5.241602952070783E-4	0.0	0.0	33.12430985561131	0.0
28	5.241602952070783E-4	0.0	0.0	33.655808394951286	0.0
29	6.988803936094377E-4	0.0	0.0	34.248808408928895	0.0
30	6.988803936094377E-4	0.0	0.0	34.96306417119774	0.0
31	6.988803936094377E-4	0.0	0.0	35.61511957843535	0.0
32	8.736004920117971E-4	0.0	0.0	36.21056567379059	0.0
33	0.0010483205904141565	0.0	0.0	36.75481878031394	0.0
34	0.0010483205904141565	0.0	0.0	37.30431348978936	0.0
35	0.0010483205904141565	0.0	0.0	37.93225752344744	0.0
36	0.0010483205904141565	0.0	0.0	38.477034790265996	0.0
37	0.0010483205904141565	0.0	0.0	39.0324699830871	0.0
38	0.0010483205904141565	0.0	0.0	39.53112114392743	0.0
39	0.0010483205904141565	0.0	0.0	40.069084326908296	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	25	3.8883416E-5	45.000004	1
TCACGAC	25	3.8883416E-5	45.000004	24
CTTAATT	25	3.8883416E-5	45.000004	19
CTTCGCA	25	3.8883416E-5	45.000004	12
GTGCAAC	25	3.8883416E-5	45.000004	41
AATCCTC	30	2.1635842E-6	45.000004	25
GTACCTT	25	3.8883416E-5	45.000004	10
AACCGAT	35	1.210501E-7	45.000004	16
CTGTCGG	30	2.1635842E-6	45.000004	32
TCGTTAG	45	3.8380676E-10	45.000004	1
GTACCGA	35	1.210501E-7	45.000004	23
ACACGTC	25	3.8883416E-5	45.000004	23
TCGCAAA	25	3.8883416E-5	45.000004	14
CAAACGA	30	2.1635842E-6	45.000004	39
GTATTCG	25	3.8883416E-5	45.000004	10
GGTCGTT	30	2.1635842E-6	45.000004	18
TACTTAT	25	3.8883416E-5	45.000004	31
CCTAGTG	25	3.8883416E-5	45.000004	41
ACGTTCA	50	2.1827873E-11	45.000004	44
CGGCCCA	25	3.8883416E-5	45.000004	25
>>END_MODULE