FastQCFastQC Report
Sat 14 Jan 2017
SRR2935049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378021
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC75930.22477657776550233No Hit
CCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC54320.16080421051260485No Hit
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT41170.12187609254057331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCAG1550.040.645161
CCGGTAT3600.040.62542
TACGGGT2550.040.5882344
AATAGCG1400.040.178571
ATACGAC403.460882E-739.37500410
GTAACGA403.460882E-739.37500437
TCGTTAC301.1403907E-437.49999638
TACCGGT3850.037.402640
TAGGGAC22050.037.3469435
TGTAGCG2750.036.8181841
GCGCTAA250.002107686136.044
CGTTTTT22750.035.9010961
GCCCGGT4600.035.7065231
ACCGGTA4100.035.6707341
AACGGGC7250.035.0689664
CGCTTAA458.7065746E-735.019
ATAACGG3100.034.8387072
CGGTCTA2850.034.73684331
GTACGAG2550.034.4117661
AGGGCGA22700.034.1960336
Produced by FastQC (version 0.11.5)