##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935049.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3378021
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39096559790481	33.0	31.0	34.0	31.0	34.0
2	32.56353498098443	34.0	31.0	34.0	31.0	34.0
3	32.57034666155125	34.0	31.0	34.0	31.0	34.0
4	36.0726647347663	37.0	35.0	37.0	35.0	37.0
5	35.99341182307629	37.0	35.0	37.0	35.0	37.0
6	35.9349918191746	37.0	35.0	37.0	35.0	37.0
7	36.16756645384975	37.0	35.0	37.0	35.0	37.0
8	35.92886604316551	37.0	36.0	37.0	35.0	37.0
9	37.662370956249234	39.0	38.0	39.0	35.0	39.0
10	37.48737707669668	39.0	37.0	39.0	35.0	39.0
11	37.38039816803981	39.0	37.0	39.0	35.0	39.0
12	37.16883850041193	39.0	37.0	39.0	34.0	39.0
13	37.06145106853983	39.0	37.0	39.0	33.0	39.0
14	38.080626200962044	40.0	37.0	41.0	33.0	41.0
15	38.19786881135434	40.0	37.0	41.0	33.0	41.0
16	38.31405044551233	40.0	37.0	41.0	34.0	41.0
17	38.35251764272632	40.0	37.0	41.0	34.0	41.0
18	38.34948154555582	40.0	37.0	41.0	34.0	41.0
19	38.32615664615466	40.0	37.0	41.0	34.0	41.0
20	38.269748175040945	40.0	37.0	41.0	34.0	41.0
21	38.203602937933184	40.0	37.0	41.0	34.0	41.0
22	38.183196611270326	40.0	36.0	41.0	34.0	41.0
23	38.011208337662794	40.0	36.0	41.0	34.0	41.0
24	37.790547187243654	40.0	36.0	41.0	33.0	41.0
25	37.73087526690924	40.0	35.0	41.0	33.0	41.0
26	37.86898778900427	40.0	36.0	41.0	34.0	41.0
27	37.89417294919126	40.0	36.0	41.0	34.0	41.0
28	37.84374579080473	40.0	36.0	41.0	34.0	41.0
29	37.840175949172604	40.0	36.0	41.0	34.0	41.0
30	37.74265228072886	40.0	36.0	41.0	33.0	41.0
31	37.63797116714194	40.0	35.0	41.0	33.0	41.0
32	37.56232450893585	40.0	35.0	41.0	33.0	41.0
33	37.48677968550225	40.0	35.0	41.0	33.0	41.0
34	37.34195021286132	40.0	35.0	41.0	33.0	41.0
35	37.214921695276615	39.0	35.0	41.0	32.0	41.0
36	37.15619589102614	39.0	35.0	41.0	32.0	41.0
37	37.082632109155035	39.0	35.0	41.0	32.0	41.0
38	37.00551417531152	39.0	35.0	41.0	32.0	41.0
39	36.959703033225665	39.0	35.0	41.0	32.0	41.0
40	36.870958469470736	39.0	35.0	41.0	32.0	41.0
41	36.74035507772154	39.0	35.0	41.0	31.0	41.0
42	36.7212062920864	39.0	35.0	41.0	31.0	41.0
43	36.66117025323407	39.0	35.0	41.0	31.0	41.0
44	36.56274368927843	39.0	35.0	41.0	31.0	41.0
45	36.53684420552744	38.0	35.0	41.0	31.0	41.0
46	36.41278132966018	38.0	35.0	40.0	31.0	41.0
47	36.33150800424272	38.0	35.0	40.0	31.0	41.0
48	36.23780728420575	38.0	35.0	40.0	31.0	41.0
49	36.16874199420312	38.0	35.0	40.0	31.0	41.0
50	36.07903532867321	38.0	35.0	40.0	31.0	41.0
51	35.07020382644158	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	21.0
9	63.0
10	70.0
11	61.0
12	61.0
13	59.0
14	94.0
15	142.0
16	268.0
17	470.0
18	828.0
19	1527.0
20	2422.0
21	3739.0
22	5774.0
23	8171.0
24	11355.0
25	15178.0
26	19468.0
27	24156.0
28	29147.0
29	35223.0
30	44946.0
31	58244.0
32	78581.0
33	119492.0
34	236246.0
35	275698.0
36	252669.0
37	403560.0
38	690746.0
39	1058535.0
40	1003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64692877871393	22.162591647594848	25.36514722673423	10.82533234695699
2	30.594510809731496	24.207102324112252	26.90892093329201	18.289465932864243
3	32.08627181417759	23.559445012331185	26.630769909364094	17.723513264127135
4	28.701124119713878	26.06398835294393	25.927488313423748	19.307399213918444
5	26.097558304107643	29.714261693458983	24.06947736559364	20.118702636839735
6	27.66015960232337	33.818055009131086	22.84183550072661	15.679949887818934
7	82.90747748459823	5.658194546451902	6.695251450479438	4.73907651847043
8	84.2891148397242	4.199026589828779	6.602504839371928	4.909353731075088
9	78.66875309537744	6.886102839502774	8.505157309560834	5.93998675555895
10	46.1546568242175	22.94799233042068	15.125897677959966	15.77145316740186
11	32.72128858879208	22.655335772039308	22.964481274687163	21.65889436448145
12	28.97335451733426	20.289601515206684	29.208492191137946	21.528551776321105
13	26.347882384390147	21.73352385908791	30.974141368570535	20.944452387951408
14	21.54524794250835	23.97498416972541	31.863567455619723	22.616200432146513
15	20.054493444534536	22.72765622238583	34.58045405875215	22.637396274327486
16	25.317012534853987	22.530380953818817	29.403043971603495	22.7495625397237
17	26.1574158360768	22.622298677243275	26.449539538090498	24.770745948589425
18	26.109636381775008	22.5161418475492	28.90662905884836	22.46759271182743
19	26.510285164005786	24.943805855558622	25.799336356997188	22.7465726234384
20	25.770710128800268	26.490776700322467	26.398118898609567	21.340394272267698
21	25.755938166162966	24.329008019784364	29.206271956272623	20.708781857780043
22	24.71864443708313	22.029703190122262	28.519568113993373	24.732084258801233
23	23.69259989798761	22.94583130181843	28.645588644949214	24.715980155244743
24	23.988335182048896	24.20067844456858	27.670165460783103	24.140820912599416
25	24.60473158692619	24.800733920837082	25.48077705852036	25.113757433716366
26	22.404419629126046	25.048926575648878	25.700106660082927	26.846547135142146
27	22.19104025700255	24.889543315450084	27.798139798420436	25.12127662912694
28	20.671718737094885	25.522132633278478	26.64453536552911	27.16161326409753
29	22.248825569764072	24.894723863469174	26.357355386482205	26.49909518028455
30	24.4064498118869	25.02506645162952	25.937138934305025	24.631344802178553
31	24.88794474634705	26.3404519983742	24.748188362357723	24.023414892921032
32	25.99039496794129	25.287971862815535	23.198257204440115	25.523375964803062
33	25.553275127656107	24.80798668806381	24.25810851975165	25.38062966452843
34	23.129104289168126	25.02820438357251	26.226124704375724	25.616566622883635
35	22.795180965423246	26.792758245138202	24.943006571007107	25.46905421843144
36	24.615329508016675	26.819430666653645	24.561185380434285	24.0040544448954
37	23.375372740429974	28.104206575388368	25.29261955446695	23.227801129714706
38	23.471760536716616	27.059156825845665	24.600646354773996	24.868436282663726
39	23.450091044431044	24.068974112357502	25.709224424596535	26.771710418614923
40	25.408160576858464	23.14402426746311	26.285153348661833	25.162661807016594
41	21.958655674431864	25.092176750825406	25.987641876708285	26.96152569803444
42	21.57710091204288	24.880988010435694	28.58957952007995	24.95233155744147
43	21.939265623274693	23.711457092777103	28.594730464967505	25.7545468189807
44	22.52327620224978	23.36957052664859	26.799566965391868	27.307586305709762
45	23.04920543714796	22.822297433911746	25.47228095976905	28.656216169171238
46	23.34295731139623	24.060033966633128	26.47828417881357	26.118724543157075
47	20.619439606799368	25.397177815057987	29.069209457253226	24.914173120889423
48	21.563572280930167	24.849727103531922	27.96871304234047	25.617987573197443
49	22.87019530073969	23.62371341089946	28.645470232423065	24.86062105593778
50	21.842581795672675	23.441417326890505	27.809596210325516	26.906404667111307
51	21.562536171326347	23.770811371510124	26.209813378898474	28.456839078265055
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1073.0
1	2011.5
2	2950.0
3	4913.0
4	6876.0
5	5129.5
6	3383.0
7	3512.0
8	3641.0
9	3839.5
10	4038.0
11	4193.5
12	4349.0
13	4292.0
14	4235.0
15	4204.5
16	4174.0
17	4559.5
18	4945.0
19	5584.0
20	6223.0
21	6644.5
22	7066.0
23	8734.5
24	10403.0
25	11557.5
26	16028.5
27	19345.0
28	25072.5
29	30800.0
30	34233.5
31	37667.0
32	46548.5
33	55430.0
34	67540.0
35	79650.0
36	84491.5
37	89333.0
38	98091.5
39	106850.0
40	115755.0
41	124660.0
42	135942.0
43	147224.0
44	164662.5
45	182101.0
46	208306.0
47	234511.0
48	267167.0
49	299823.0
50	303790.5
51	307758.0
52	275717.5
53	243677.0
54	225501.5
55	207326.0
56	206196.0
57	205066.0
58	200483.0
59	195900.0
60	192334.0
61	188768.0
62	169613.0
63	150458.0
64	135108.5
65	119759.0
66	104296.0
67	88833.0
68	76984.0
69	65135.0
70	55480.5
71	45826.0
72	39214.0
73	32602.0
74	27876.0
75	16676.5
76	10203.0
77	7789.5
78	5376.0
79	3865.5
80	2355.0
81	1960.5
82	1566.0
83	965.0
84	364.0
85	337.0
86	310.0
87	193.0
88	76.0
89	51.5
90	27.0
91	19.0
92	11.0
93	10.0
94	9.0
95	5.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3378021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.03011726833262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.88331006223802	12.239440409847353
2	12.229312009488853	4.409918592842249
3	5.35169985612122	2.8947532797235325
4	2.862039039878262	2.0641159806220464
5	1.774455971680372	1.5996824628445108
6	1.24579664624497	1.347711577457538
7	0.863820504433521	1.0902349495608636
8	0.6802952246501055	0.9812642142022469
9	0.5050721962361343	0.8195859834400626
>10	3.71798601186241	14.358284276083882
>50	1.4094927339953598	18.922379922033347
>100	1.4647937991728397	36.776023104472664
>500	0.008566523153478502	1.0439671923048928
>1k	0.0030234787600512364	1.0581710335973125
>5k	3.3594208445013736E-4	0.39446702096751557
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	7593	0.22477657776550233	No Hit
CCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	5432	0.16080421051260485	No Hit
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT	4117	0.12187609254057331	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.480156576883329E-4	0.0	0.0	0.12998735058189395	0.0
2	1.480156576883329E-4	0.0	0.0	0.6947262909259593	0.0
3	1.480156576883329E-4	0.0	0.0	1.029922549326958	0.0
4	1.480156576883329E-4	0.0	0.0	1.460174463095404	0.0
5	1.776187892259995E-4	0.0	0.0	2.639622429819116	0.0
6	1.776187892259995E-4	0.0	0.0	3.3175637451632185	0.0
7	1.776187892259995E-4	0.0	0.0	4.027210014384162	0.0
8	1.776187892259995E-4	0.0	0.0	5.083745778963482	0.0
9	1.776187892259995E-4	0.0	0.0	5.467076729244726	0.0
10	1.776187892259995E-4	0.0	0.0	6.336076655532929	0.0
11	1.776187892259995E-4	0.0	0.0	7.695896502715644	0.0
12	1.776187892259995E-4	0.0	0.0	8.69538703282188	0.0
13	2.0722192076366605E-4	0.0	0.0	9.090411220060503	0.0
14	2.0722192076366605E-4	0.0	0.0	9.25829057901061	0.0
15	2.0722192076366605E-4	0.0	0.0	9.567051240948473	0.0
16	2.3682505230133264E-4	0.0	0.0	10.201002302827602	0.0
17	2.3682505230133264E-4	0.0	0.0	10.955734141380411	0.0
18	3.256344469143324E-4	0.0	0.0	11.781809526938998	0.0
19	3.256344469143324E-4	0.0	0.0	12.312179231567832	0.0
20	3.55237578451999E-4	0.0	0.0	12.834319265629196	0.0
21	3.55237578451999E-4	0.0	0.0	13.491893626475383	0.0
22	3.55237578451999E-4	0.0	0.0	14.156602342022149	0.0
23	3.55237578451999E-4	0.0	0.0	14.797273314760329	0.0
24	3.55237578451999E-4	0.0	0.0	15.340194747161133	0.0
25	3.55237578451999E-4	0.0	0.0	15.804727087250198	0.0
26	3.55237578451999E-4	0.0	0.0	16.252060007915876	0.0
27	3.55237578451999E-4	0.0	0.0	16.76898989082661	0.0
28	3.8484070998966554E-4	0.0	0.0	17.235061593755635	0.0
29	3.8484070998966554E-4	0.0	0.0	17.747314181883418	0.0
30	3.8484070998966554E-4	0.0	0.0	18.28760093557737	0.0
31	4.144438415273321E-4	0.0	0.0	18.75062351595801	0.0
32	4.144438415273321E-4	0.0	0.0	19.204765156877354	0.0
33	4.440469730649987E-4	0.0	0.0	19.664028139552716	0.0
34	4.440469730649987E-4	0.0	0.0	20.150407590716576	0.0
35	4.440469730649987E-4	0.0	0.0	20.675123097221718	0.0
36	4.440469730649987E-4	0.0	0.0	21.12985680077181	0.0
37	4.440469730649987E-4	0.0	0.0	21.625354016449275	0.0
38	4.440469730649987E-4	0.0	0.0	22.16001617515107	0.0
39	4.736501046026653E-4	0.0	0.0	22.987778939207306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCAG	155	0.0	40.64516	1
CCGGTAT	360	0.0	40.625	42
TACGGGT	255	0.0	40.588234	4
AATAGCG	140	0.0	40.17857	1
ATACGAC	40	3.460882E-7	39.375004	10
GTAACGA	40	3.460882E-7	39.375004	37
TCGTTAC	30	1.1403907E-4	37.499996	38
TACCGGT	385	0.0	37.4026	40
TAGGGAC	2205	0.0	37.346943	5
TGTAGCG	275	0.0	36.818184	1
GCGCTAA	25	0.0021076861	36.0	44
CGTTTTT	2275	0.0	35.901096	1
GCCCGGT	460	0.0	35.70652	31
ACCGGTA	410	0.0	35.67073	41
AACGGGC	725	0.0	35.068966	4
CGCTTAA	45	8.7065746E-7	35.0	19
ATAACGG	310	0.0	34.838707	2
CGGTCTA	285	0.0	34.736843	31
GTACGAG	255	0.0	34.411766	1
AGGGCGA	2270	0.0	34.196033	6
>>END_MODULE