SRR2935048.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935048.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2351230
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	8920	0.37937590112409253	Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	6118	0.2602042335288339	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT	4572	0.19445141479140707	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	2839	0.1207453120281725	Illumina PCR Primer Index 5 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	20	7.034793E-4	45.000004	1
TCCAACG	20	7.034793E-4	45.000004	21
CGTAACG	20	7.034793E-4	45.000004	39
CGCCGAT	20	7.034793E-4	45.000004	40
TGCGTAC	45	3.8562575E-10	45.0	24
TCGTGAA	30	2.1664746E-6	44.999996	32
GTCGTAC	25	3.892056E-5	44.999996	31
CGTACTA	30	2.1664746E-6	44.999996	15
GCGCTTA	25	3.892056E-5	44.999996	18
GCCCGTA	25	3.892056E-5	44.999996	36
GTACGAC	30	2.1664746E-6	44.999996	34
ACCCGTA	75	0.0	42.0	33
TCGTTAG	110	0.0	40.909092	1
ATTCGAC	50	1.0822987E-9	40.499996	11
ATTAGCG	85	0.0	39.705883	1
CCGTACA	40	3.4602817E-7	39.375004	35
CGTAGAC	40	3.4602817E-7	39.375004	35
TCGTAGA	40	3.4602817E-7	39.375004	20
CGCGTAA	40	3.4602817E-7	39.375004	31
GTCAACG	115	0.0	39.130432	1