##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935048.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2351230
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32651931116905	33.0	31.0	34.0	30.0	34.0
2	32.494159227298056	34.0	31.0	34.0	31.0	34.0
3	32.50451976199691	34.0	31.0	34.0	31.0	34.0
4	36.01533027394172	37.0	35.0	37.0	35.0	37.0
5	35.940693169107234	37.0	35.0	37.0	35.0	37.0
6	35.88296040795669	37.0	35.0	37.0	35.0	37.0
7	36.14777882214841	37.0	35.0	37.0	35.0	37.0
8	35.912955346775945	37.0	35.0	37.0	35.0	37.0
9	37.645903633417404	39.0	38.0	39.0	35.0	39.0
10	37.46292536246985	39.0	37.0	39.0	35.0	39.0
11	37.390257865032346	39.0	37.0	39.0	35.0	39.0
12	37.12047056221637	39.0	37.0	39.0	34.0	39.0
13	36.97437809146702	39.0	37.0	39.0	33.0	39.0
14	37.89118418870123	40.0	37.0	41.0	33.0	41.0
15	38.04744665558027	40.0	37.0	41.0	33.0	41.0
16	38.17769890652978	40.0	37.0	41.0	33.0	41.0
17	38.231357629836296	40.0	37.0	41.0	34.0	41.0
18	38.25642153255956	40.0	37.0	41.0	34.0	41.0
19	38.21543362410313	40.0	37.0	41.0	34.0	41.0
20	38.16109738307184	40.0	36.0	41.0	34.0	41.0
21	38.09940584289925	40.0	36.0	41.0	34.0	41.0
22	38.08641306890436	40.0	36.0	41.0	34.0	41.0
23	37.874708556797934	40.0	36.0	41.0	34.0	41.0
24	37.66960612105154	40.0	35.0	41.0	33.0	41.0
25	37.60654933800606	39.0	35.0	41.0	33.0	41.0
26	37.7680307753814	40.0	35.0	41.0	34.0	41.0
27	37.79917532525529	40.0	35.0	41.0	34.0	41.0
28	37.77774058684178	40.0	36.0	41.0	34.0	41.0
29	37.799191487008926	40.0	36.0	41.0	34.0	41.0
30	37.69703601944514	40.0	35.0	41.0	33.0	41.0
31	37.58215146965631	40.0	35.0	41.0	33.0	41.0
32	37.50224690906462	40.0	35.0	41.0	33.0	41.0
33	37.43296104592065	40.0	35.0	41.0	33.0	41.0
34	37.272851231057786	39.0	35.0	41.0	33.0	41.0
35	37.17904160800943	39.0	35.0	41.0	32.0	41.0
36	37.132213352160356	39.0	35.0	41.0	32.0	41.0
37	37.061470804642674	39.0	35.0	41.0	32.0	41.0
38	37.01543702657758	39.0	35.0	41.0	32.0	41.0
39	36.93365685194558	39.0	35.0	41.0	32.0	41.0
40	36.78752440212144	39.0	35.0	41.0	31.0	41.0
41	36.69607311917592	39.0	35.0	41.0	31.0	41.0
42	36.74467831730626	39.0	35.0	41.0	31.0	41.0
43	36.71663469758382	39.0	35.0	41.0	32.0	41.0
44	36.60918795694169	39.0	35.0	41.0	31.0	41.0
45	36.56258851749935	38.0	35.0	41.0	31.0	41.0
46	36.43233881840568	38.0	35.0	40.0	31.0	41.0
47	36.35262181921803	38.0	35.0	40.0	31.0	41.0
48	36.310252931444396	38.0	35.0	40.0	31.0	41.0
49	36.26652135265372	38.0	35.0	40.0	31.0	41.0
50	36.18653640860315	38.0	35.0	40.0	31.0	41.0
51	35.18481007812932	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	39.0
10	34.0
11	42.0
12	34.0
13	36.0
14	65.0
15	96.0
16	211.0
17	358.0
18	586.0
19	1074.0
20	1676.0
21	2646.0
22	4232.0
23	5959.0
24	8282.0
25	10877.0
26	13728.0
27	16910.0
28	20247.0
29	25126.0
30	31809.0
31	41533.0
32	57194.0
33	86487.0
34	172601.0
35	194011.0
36	171495.0
37	272560.0
38	487796.0
39	722823.0
40	651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19237165228412	21.802163123131297	24.80463417020028	10.2008310543843
2	31.724246458236756	24.13438923457084	25.18469056621428	18.956673740978125
3	32.322018688090914	24.3750292400148	25.693785805727217	17.60916626616707
4	28.227395873649115	26.1447837940142	26.226102933358288	19.40171739897841
5	26.480352836600417	29.568311054214174	24.20533082684382	19.746005282341585
6	27.34891950170762	33.11841036393717	23.181143486600632	16.35152664775458
7	81.84643782190598	5.560196152652016	7.769550405532423	4.823815619909579
8	82.09694500325362	4.532904054473616	7.702223942362083	5.667926999910685
9	76.34106403882224	7.109130114876043	9.68667463412767	6.8631312121740535
10	42.28131658748825	27.464646163922712	16.191014915597368	14.063022332991668
11	34.09555849491543	24.049454966124113	22.481297023260165	19.373689515700292
12	29.597657396341486	22.466241073820935	27.69176133342974	20.244340196407837
13	26.384190402470196	24.68163471884928	29.69815798539488	19.236016893285644
14	20.603684029210243	27.22068874589045	30.809788918991337	21.36583830590797
15	19.276804055749544	24.618688941532728	34.62213394691289	21.482373055804835
16	24.319568906487245	25.50992459266001	29.125606597397958	21.044899903454787
17	24.965018309565632	24.930313070180286	25.705354218855664	24.399314401398417
18	26.947725233175827	23.09986687818716	28.711270271304805	21.241137617332203
19	25.785567554003645	25.297865372592216	25.915244361461877	23.001322711942258
20	25.8282260774148	27.5697826244136	26.922419329457348	19.679571968714246
21	24.27223198070797	26.61976922717046	29.06657366569838	20.04142512642319
22	23.930283298528852	23.1614516657239	29.08809431659174	23.820170719155506
23	21.39475933872909	26.39001713996504	29.37143537637747	22.843788144928396
24	23.307843128915504	25.148539275187883	28.261463149075166	23.28215444682145
25	22.489675616592166	27.47672494821859	26.025697188280176	24.007902246909065
26	21.997167440020753	25.389391935284937	26.550443810261008	26.062996814433298
27	21.709062916005664	26.29487544816968	27.80297971699919	24.193081918825467
28	18.159856755825672	26.349527694015475	27.643403665315603	27.847211884843254
29	20.550009994768693	26.75348647303752	26.851180020670036	25.845323511523755
30	23.887752367909563	25.7043334765208	27.089906134236124	23.318008021333515
31	23.78627356745193	27.066514122395514	25.021499385428054	24.125712924724507
32	24.44597083228778	26.6006728393224	25.557304049369904	23.396052279019916
33	24.493265227136433	26.27981949873045	24.21213577574291	25.014779498390205
34	20.507479064149404	26.161753635331298	28.28047447506199	25.050292825457316
35	20.164807356149762	28.616809074399356	26.826469549980224	24.391914019470658
36	23.277773760967662	28.884966591953997	23.86316949001161	23.974090157066726
37	20.46847820077151	29.62164484121077	25.927578331341465	23.98229862667625
38	22.359828685411465	28.00908460678028	25.17142091586106	24.459665791947195
39	23.10850065710288	25.187114829259578	25.56678844689801	26.137596066739537
40	25.548415084870474	24.781242158359667	26.449688035623907	23.22065472114595
41	20.521046431016956	26.815624162672304	24.32518298932899	28.33814641698175
42	21.667595258651854	24.86285901421809	27.30073195731596	26.168813769814097
43	22.04122948414234	23.662636152141644	28.07339137387665	26.22274298983936
44	22.55330188879863	23.796736176384275	26.47903437775122	27.170927557065877
45	22.10634433892048	23.272372332779014	26.201605117321574	28.419678210978933
46	22.83107139667323	24.439123352458076	26.680843643539763	26.04896160732893
47	19.087541414493693	26.49383514160673	29.75408615915925	24.664537284740327
48	20.29703601944514	26.09842507963917	27.887871454515295	25.716667446400393
49	23.16600247530016	23.191563564602358	29.080693934663987	24.561740025433494
50	21.2378202047439	24.96506084049625	27.643956567413653	26.153162387346196
51	20.224690906461724	24.59448884201035	25.61586914083267	29.564951110695254
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1095.0
1	1721.0
2	2347.0
3	3408.0
4	4469.0
5	3435.5
6	2402.0
7	2516.5
8	2631.0
9	2664.5
10	2698.0
11	2833.0
12	2968.0
13	3117.0
14	3266.0
15	3542.0
16	3818.0
17	3695.5
18	3573.0
19	4135.5
20	4698.0
21	5233.5
22	5769.0
23	6501.0
24	7233.0
25	8963.0
26	12332.5
27	13972.0
28	19701.0
29	25430.0
30	28998.0
31	32566.0
32	35597.0
33	38628.0
34	46170.5
35	53713.0
36	59309.5
37	64906.0
38	69829.0
39	74752.0
40	81272.5
41	87793.0
42	101675.5
43	115558.0
44	129061.5
45	142565.0
46	164582.5
47	186600.0
48	213536.0
49	240472.0
50	245569.5
51	250667.0
52	223583.5
53	196500.0
54	177765.0
55	159030.0
56	148394.5
57	137759.0
58	126383.5
59	115008.0
60	107483.5
61	99959.0
62	88222.0
63	76485.0
64	65497.0
65	54509.0
66	48246.0
67	41983.0
68	35256.5
69	28530.0
70	25041.0
71	21552.0
72	18065.5
73	14579.0
74	12079.0
75	7619.0
76	5659.0
77	4046.0
78	2433.0
79	1942.5
80	1452.0
81	1055.0
82	658.0
83	400.5
84	143.0
85	108.0
86	73.0
87	51.5
88	30.0
89	22.0
90	14.0
91	11.5
92	9.0
93	5.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2351230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.507751188633282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.36457367733425	12.946235910371584
2	12.718154026303418	4.962051686476833
3	5.68689100846925	3.328153644902818
4	3.2705983460901678	2.552080750939301
5	2.0068047118941235	1.9574123501903729
6	1.3418808032224836	1.5706246100440555
7	0.9459630997669468	1.2917528948717316
8	0.7206331623574036	1.124634594363696
9	0.568868459424917	0.9987609928970168
>10	3.9622942522493516	16.13524316597627
>50	1.239507340491305	17.871621653039597
>100	1.1591970362447674	31.68196721605092
>500	0.00909086548812628	1.1710217623961545
>1k	0.005099753810412304	1.7579816602913203
>5k	4.434568530793308E-4	0.6504571071884673
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	8920	0.37937590112409253	Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	6118	0.2602042335288339	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT	4572	0.19445141479140707	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	2839	0.1207453120281725	Illumina PCR Primer Index 5 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1265465309646442E-4	0.0	0.0	0.20716816304657562	0.0
2	2.1265465309646442E-4	0.0	0.0	1.1201371197203167	0.0
3	2.1265465309646442E-4	0.0	0.0	1.6195778379826729	0.0
4	2.1265465309646442E-4	0.0	0.0	2.2501839462749285	0.0
5	2.1265465309646442E-4	0.0	0.0	3.920245998902702	0.0
6	2.1265465309646442E-4	0.0	0.0	4.767036827532824	0.0
7	2.1265465309646442E-4	0.0	0.0	5.675880283936493	0.0
8	2.1265465309646442E-4	0.0	0.0	7.035253888390332	0.0
9	2.1265465309646442E-4	0.0	0.0	7.497054733054614	0.0
10	2.1265465309646442E-4	0.0	0.0	8.678478923797332	0.0
11	2.1265465309646442E-4	0.0	0.0	10.339311764480719	0.0
12	2.5518558371575727E-4	0.0	0.0	11.703661487817016	0.0
13	2.5518558371575727E-4	0.0	0.0	12.22415501673592	0.0
14	2.9771651433505014E-4	0.0	0.0	12.437787881236629	0.0
15	2.9771651433505014E-4	0.0	0.0	12.853017356872785	0.0
16	2.9771651433505014E-4	0.0	0.0	13.64787791921675	0.0
17	2.9771651433505014E-4	0.0	0.0	14.609204544004628	0.0
18	6.80494889908686E-4	0.0	0.0	15.63573108543188	0.0
19	6.80494889908686E-4	0.0	0.0	16.290622355107754	0.0
20	6.80494889908686E-4	0.0	0.0	16.96856538917928	0.0
21	6.80494889908686E-4	0.0	0.0	17.770698740659146	0.0
22	6.80494889908686E-4	0.0	0.0	18.587845510647618	0.0
23	6.80494889908686E-4	0.0	0.0	19.371137659863134	0.0
24	6.80494889908686E-4	0.0	0.0	19.969632915537826	0.0
25	6.80494889908686E-4	0.0	0.0	20.528489343875332	0.0
26	6.80494889908686E-4	0.0	0.0	21.062422646869937	0.0
27	6.80494889908686E-4	0.0	0.0	21.72143941681588	0.0
28	6.80494889908686E-4	0.0	0.0	22.28752610335867	0.0
29	6.80494889908686E-4	0.0	0.0	22.885425926004686	0.0
30	6.80494889908686E-4	0.0	0.0	23.58050892511579	0.0
31	6.80494889908686E-4	0.0	0.0	24.106361351292726	0.0
32	7.23025820527979E-4	0.0	0.0	24.613500167997177	0.0
33	7.23025820527979E-4	0.0	0.0	25.15338780127848	0.0
34	7.655567511472719E-4	0.0	0.0	25.711351080072983	0.0
35	7.655567511472719E-4	0.0	0.0	26.325497718215573	0.0
36	7.655567511472719E-4	0.0	0.0	26.839739200333444	0.0
37	7.655567511472719E-4	0.0	0.0	27.38536850924835	0.0
38	7.655567511472719E-4	0.0	0.0	28.00304521463234	0.0
39	7.655567511472719E-4	0.0	0.0	28.98049106212493	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	20	7.034793E-4	45.000004	1
TCCAACG	20	7.034793E-4	45.000004	21
CGTAACG	20	7.034793E-4	45.000004	39
CGCCGAT	20	7.034793E-4	45.000004	40
TGCGTAC	45	3.8562575E-10	45.0	24
TCGTGAA	30	2.1664746E-6	44.999996	32
GTCGTAC	25	3.892056E-5	44.999996	31
CGTACTA	30	2.1664746E-6	44.999996	15
GCGCTTA	25	3.892056E-5	44.999996	18
GCCCGTA	25	3.892056E-5	44.999996	36
GTACGAC	30	2.1664746E-6	44.999996	34
ACCCGTA	75	0.0	42.0	33
TCGTTAG	110	0.0	40.909092	1
ATTCGAC	50	1.0822987E-9	40.499996	11
ATTAGCG	85	0.0	39.705883	1
CCGTACA	40	3.4602817E-7	39.375004	35
CGTAGAC	40	3.4602817E-7	39.375004	35
TCGTAGA	40	3.4602817E-7	39.375004	20
CGCGTAA	40	3.4602817E-7	39.375004	31
GTCAACG	115	0.0	39.130432	1
>>END_MODULE