##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935047.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1229529
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17520204891466	33.0	31.0	34.0	30.0	34.0
2	32.33907130291355	34.0	31.0	34.0	30.0	34.0
3	32.36309106983243	34.0	31.0	34.0	30.0	34.0
4	35.90560613047761	37.0	35.0	37.0	35.0	37.0
5	35.82903615937485	37.0	35.0	37.0	35.0	37.0
6	35.766391032663726	37.0	35.0	37.0	35.0	37.0
7	36.0687442101813	37.0	35.0	37.0	35.0	37.0
8	35.84214687087494	37.0	35.0	37.0	35.0	37.0
9	37.55108094237712	39.0	38.0	39.0	35.0	39.0
10	37.35837544295417	39.0	37.0	39.0	34.0	39.0
11	37.271418567597834	39.0	37.0	39.0	34.0	39.0
12	37.10268647587816	39.0	37.0	39.0	34.0	39.0
13	36.97544100220491	39.0	37.0	39.0	33.0	39.0
14	38.03769166892363	40.0	37.0	41.0	33.0	41.0
15	38.14122725043492	40.0	37.0	41.0	33.0	41.0
16	38.20225712447612	40.0	37.0	41.0	34.0	41.0
17	38.228905540251596	40.0	37.0	41.0	34.0	41.0
18	38.24119967890143	40.0	37.0	41.0	34.0	41.0
19	38.220380324498244	40.0	37.0	41.0	34.0	41.0
20	38.17459531251398	40.0	36.0	41.0	34.0	41.0
21	38.08220627573648	40.0	36.0	41.0	34.0	41.0
22	37.98857123337473	40.0	36.0	41.0	34.0	41.0
23	37.796736799213356	40.0	36.0	41.0	33.0	41.0
24	37.554777479831706	39.0	35.0	41.0	33.0	41.0
25	37.511557677777425	39.0	35.0	41.0	33.0	41.0
26	37.70232259670166	39.0	36.0	41.0	33.0	41.0
27	37.71015567749927	39.0	36.0	41.0	33.0	41.0
28	37.72665955825361	40.0	36.0	41.0	33.0	41.0
29	37.781029158319974	40.0	36.0	41.0	34.0	41.0
30	37.661891667459656	40.0	36.0	41.0	33.0	41.0
31	37.59687083427882	40.0	36.0	41.0	33.0	41.0
32	37.55708324081823	40.0	36.0	41.0	33.0	41.0
33	37.476934663598826	40.0	35.0	41.0	33.0	41.0
34	37.39032507569972	40.0	35.0	41.0	33.0	41.0
35	37.340150577985554	40.0	35.0	41.0	33.0	41.0
36	37.25460806536487	39.0	35.0	41.0	33.0	41.0
37	37.172000009759834	39.0	35.0	41.0	33.0	41.0
38	37.120648638625035	39.0	35.0	41.0	32.0	41.0
39	37.08878684439326	39.0	35.0	41.0	32.0	41.0
40	37.01987509038014	39.0	35.0	41.0	32.0	41.0
41	36.98607678224751	39.0	35.0	41.0	32.0	41.0
42	36.952655854396276	39.0	35.0	41.0	32.0	41.0
43	36.83307429104966	39.0	35.0	41.0	32.0	41.0
44	36.695363834443924	39.0	35.0	41.0	31.0	41.0
45	36.73540518361096	39.0	35.0	41.0	31.0	41.0
46	36.63152068800329	39.0	35.0	41.0	31.0	41.0
47	36.56176552159404	39.0	35.0	41.0	31.0	41.0
48	36.53615164831411	39.0	35.0	41.0	31.0	41.0
49	36.49103355837886	39.0	35.0	41.0	31.0	41.0
50	36.40409132277482	39.0	35.0	40.0	31.0	41.0
51	35.390012760984085	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	12.0
10	15.0
11	14.0
12	9.0
13	12.0
14	21.0
15	41.0
16	83.0
17	169.0
18	293.0
19	515.0
20	957.0
21	1506.0
22	2393.0
23	3609.0
24	5066.0
25	6272.0
26	7577.0
27	8658.0
28	10401.0
29	12540.0
30	16191.0
31	21125.0
32	28135.0
33	40660.0
34	77613.0
35	114998.0
36	87937.0
37	135669.0
38	260363.0
39	386376.0
40	292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.19531056201196	22.783439837531283	25.911385579356	11.109864021100762
2	28.74336432894222	25.64372210822193	26.272174141480193	19.340739421355657
3	29.63411192415958	24.87285781791239	27.963390859426656	17.529639398501377
4	28.07668627580155	26.588474123017843	27.252386889613827	18.082452711566788
5	24.31752321417388	29.93739879254576	25.46064387257234	20.28443412070801
6	25.143855899291516	32.599231087676664	25.638110203175362	16.618802809856454
7	78.41636919503323	6.7634028965563235	8.892104212263394	5.928123696147061
8	78.3251147390586	4.699523150734956	9.21035616077376	7.765005949432668
9	72.12298367911615	7.939869657405396	12.029647125037311	7.907499538441143
10	39.34262632276262	29.01867300405277	17.674898274054538	13.963802399130074
11	28.054157323658085	23.894678368708668	27.09696151941109	20.954202788222158
12	24.94442994024541	21.351753394999225	33.55447492495094	20.14934173980443
13	21.561183184780514	25.9234227090211	33.65654653123269	18.858847574965697
14	16.69330288264856	30.96852534588448	31.074744881983264	21.263426889483696
15	15.985308195252005	26.129517888557324	38.818767186459205	19.066406729731465
16	18.26170834522813	26.16091202403522	33.57952516776749	21.99785446296915
17	18.108397605912508	25.75864416374075	28.431537605050387	27.701420625296354
18	19.952843731217403	25.202170912601492	32.994260403780636	21.850724952400473
19	20.239457548378283	27.563644289805282	28.500100444967135	23.696797716849296
20	21.655690919042982	28.440321456427625	29.06747217837074	20.83651544615865
21	20.888161238978505	28.76142002343987	30.26459725634776	20.08582148123387
22	18.13190254154233	26.325283909529585	29.000047985854742	26.54276556307334
23	18.783615514558825	26.88118783696847	28.998502678668007	25.336693969804696
24	20.815694465116316	24.982574628170624	30.476223008973353	23.725507897739703
25	17.44806344543317	27.886776155747445	28.316452885617178	26.348707513202207
26	17.24343224112648	28.730188551876367	27.95062174214679	26.075757464850362
27	19.47794643314635	28.910176173152486	28.285546741882463	23.326330651818704
28	15.511386880667313	26.529020462307113	32.1966378995534	25.762954757472173
29	17.18235194127182	24.910921173880404	31.523127962008218	26.383598922839557
30	19.540084048444566	27.610247501278945	28.380135808102125	24.46953264217436
31	18.985562764278026	27.653028110764367	28.893177794098392	24.468231330859215
32	19.918358981366037	26.564237199773245	27.22449002829539	26.29291379056533
33	21.14720352264973	25.944406353977822	26.881594496754445	26.026795626618
34	16.692896222862576	23.517298087316362	32.95945032610048	26.83035536372058
35	16.877601097656093	24.55810314356148	28.208769374288856	30.355526384493576
36	22.107408609312998	25.113031087514	28.47309823517786	24.306462067995145
37	19.309101290006172	24.9135237965107	33.67443956181595	22.10293535166718
38	18.3586560382065	25.73985648162833	29.088862483113452	26.812624997051714
39	21.97532551082569	22.859566549467317	30.558042957913152	24.60706498179384
40	21.2103984533915	21.36891443796771	30.758770228274408	26.66191688036638
41	18.72196589100379	23.68557390675616	27.208955624470832	30.38350457776921
42	21.881793760049582	22.552538411050087	28.24780871374323	27.317859115157106
43	22.904949781583028	22.82825374594662	29.7080426732513	24.55875379921905
44	19.136352212920556	23.284526025819645	30.02434265478895	27.55477910647085
45	19.789935820952575	21.76199178709896	29.421835515876403	29.02623687607206
46	21.92555035302136	22.396137057360992	29.94634530783739	25.73196728178026
47	17.572094680158013	22.4343630772434	33.45004469191048	26.543497550688112
48	18.915942608917724	21.09816035246017	31.78900213008396	28.196894908538145
49	22.05397351343482	18.530673127677346	34.30240360333103	25.1129497555568
50	19.36473234872866	19.638414384695277	33.06046461693868	27.936388649637383
51	18.32669257902823	19.804900901076756	29.078939984335467	32.789466535559555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1683.0
1	1760.5
2	1838.0
3	2204.5
4	2571.0
5	2080.5
6	1590.0
7	1711.5
8	1833.0
9	2025.0
10	2217.0
11	2283.0
12	2349.0
13	2570.5
14	2792.0
15	2848.5
16	2905.0
17	3086.5
18	3268.0
19	3743.0
20	4218.0
21	4334.5
22	4451.0
23	4363.5
24	4276.0
25	5281.5
26	7877.0
27	9467.0
28	11020.5
29	12574.0
30	14767.5
31	16961.0
32	18649.0
33	20337.0
34	23281.5
35	26226.0
36	28929.0
37	31632.0
38	35016.5
39	38401.0
40	45015.0
41	51629.0
42	62759.5
43	73890.0
44	85875.5
45	97861.0
46	115700.0
47	133539.0
48	154749.5
49	175960.0
50	168888.5
51	161817.0
52	129830.0
53	97843.0
54	83849.0
55	69855.0
56	58806.0
57	47757.0
58	41955.0
59	36153.0
60	33033.0
61	29913.0
62	25998.0
63	22083.0
64	18587.5
65	15092.0
66	11362.5
67	7633.0
68	5910.5
69	4188.0
70	3397.0
71	2606.0
72	2162.0
73	1718.0
74	1356.0
75	818.5
76	643.0
77	458.5
78	274.0
79	197.5
80	121.0
81	92.5
82	64.0
83	37.0
84	10.0
85	7.5
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1229529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.798022408604158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.05549501985506	13.322276208171774
2	12.554869618253054	5.22232919315061
3	6.2246022947188715	3.883782540306359
4	3.7663277330349283	3.1332867435923095
5	2.44241577488302	2.539870900857267
6	1.6795835559242884	2.095920985994184
7	1.2846285168926248	1.8702412876745318
8	0.9701732705406083	1.6142148336745883
9	0.7378062088044184	1.3810419057529417
>10	4.362012561330817	17.278599833570826
>50	0.9948502547222299	15.399519723451691
>100	0.910627993588645	27.14490407458843
>500	0.01067605550451274	1.5372627601377633
>1k	0.004744913557561217	1.6654707868351781
>5k	0.0011862283893903042	1.9112782222415747
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	9935	0.8080329947483955	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	7459	0.6066550687295704	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	5847	0.4755479537286229	No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	3677	0.2990576066119628	No Hit
ACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2730	0.22203624314676593	No Hit
GCCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1805	0.1468041827398947	No Hit
ACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1691	0.1375323396194803	No Hit
AGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1676	0.13631236026153104	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1399	0.11378340811806797	No Hit
CGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1388	0.11288875658890518	No Hit
GGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTG	1316	0.10703285567074872	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6266391439323513E-4	0.0	0.0	0.5022248356891135	0.0
2	1.6266391439323513E-4	0.0	0.0	2.5444702808961805	0.0
3	1.6266391439323513E-4	0.0	0.0	3.5536372057918113	0.0
4	1.6266391439323513E-4	0.0	0.0	4.765727363893003	0.0
5	1.6266391439323513E-4	0.0	0.0	7.763704638117523	0.0
6	1.6266391439323513E-4	0.0	0.0	9.206126898999536	0.0
7	1.6266391439323513E-4	0.0	0.0	10.771035087419655	0.0
8	1.6266391439323513E-4	0.0	0.0	13.20212861998375	0.0
9	1.6266391439323513E-4	0.0	0.0	14.037082492564226	0.0
10	1.6266391439323513E-4	0.0	0.0	15.899014988666393	0.0
11	1.6266391439323513E-4	0.0	0.0	18.733108369139728	0.0
12	1.6266391439323513E-4	0.0	0.0	20.95265748103542	0.0
13	2.439958715898527E-4	0.0	0.0	21.782568772269705	0.0
14	2.439958715898527E-4	0.0	0.0	22.092362197231623	0.0
15	2.439958715898527E-4	0.0	0.0	22.664288520238237	0.0
16	2.439958715898527E-4	0.0	0.0	23.88979845127687	0.0
17	2.439958715898527E-4	0.0	0.0	25.411275374553995	0.0
18	3.2532782878647026E-4	0.0	0.0	27.238072465147223	0.0
19	3.2532782878647026E-4	0.0	0.0	28.263099121696193	0.0
20	4.0665978598308783E-4	0.0	0.0	29.203459210803487	0.0
21	4.0665978598308783E-4	0.0	0.0	30.233203120869863	0.0
22	4.0665978598308783E-4	0.0	0.0	31.32345800709052	0.0
23	4.0665978598308783E-4	0.0	0.0	32.3483219997251	0.0
24	4.0665978598308783E-4	0.0	0.0	33.174329357013946	0.0
25	4.0665978598308783E-4	0.0	0.0	33.858656444866284	0.0
26	4.0665978598308783E-4	0.0	0.0	34.495892329501785	0.0
27	4.0665978598308783E-4	0.0	0.0	35.12971227193503	0.0
28	4.879917431797054E-4	0.0	0.0	35.78549184281135	0.0
29	4.879917431797054E-4	0.0	0.0	36.4407834219445	0.0
30	4.879917431797054E-4	0.0	0.0	37.1765936387023	0.0
31	4.879917431797054E-4	0.0	0.0	37.846931629916824	0.0
32	4.879917431797054E-4	0.0	0.0	38.49246337418638	0.0
33	4.879917431797054E-4	0.0	0.0	39.102046393375026	0.0
34	4.879917431797054E-4	0.0	0.0	39.71537068259472	0.0
35	4.879917431797054E-4	0.0	0.0	40.376030170902844	0.0
36	4.879917431797054E-4	0.0	0.0	40.970322782138524	0.0
37	4.879917431797054E-4	0.0	0.0	41.5695766427632	0.0
38	4.879917431797054E-4	0.0	0.0	42.1829009319829	0.0
39	4.879917431797054E-4	0.0	0.0	42.81883550530325	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	30	2.1656251E-6	45.000004	1
CTCCCGG	30	2.1656251E-6	45.000004	2
TTCGTTC	30	2.1656251E-6	45.000004	14
GTTAGGT	75	0.0	45.000004	10
AGGCGTA	30	2.1656251E-6	45.000004	42
GGAACGT	60	0.0	45.000004	8
TTACGCG	30	2.1656251E-6	45.000004	1
GCGGATA	30	2.1656251E-6	45.000004	1
GGCGAGT	30	2.1656251E-6	45.000004	2
CCTGCGT	30	2.1656251E-6	45.000004	43
CCGTTAG	30	2.1656251E-6	45.000004	45
GTACGAG	75	0.0	45.000004	1
CGAACTT	20	7.0334796E-4	45.0	24
AAATCCG	20	7.0334796E-4	45.0	1
CGAACCG	20	7.0334796E-4	45.0	2
CGTATTA	35	1.212029E-7	45.0	45
GCGACCG	45	3.8562575E-10	45.0	14
GATCGTC	35	1.212029E-7	45.0	20
ATTAGCG	20	7.0334796E-4	45.0	1
CGTATAG	20	7.0334796E-4	45.0	14
>>END_MODULE