##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935046.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1437034
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.303975410463494	33.0	31.0	34.0	30.0	34.0
2	32.4750604369834	34.0	31.0	34.0	31.0	34.0
3	32.477089616529604	34.0	31.0	34.0	31.0	34.0
4	36.00378905439955	37.0	35.0	37.0	35.0	37.0
5	35.91650789055792	37.0	35.0	37.0	35.0	37.0
6	35.863527933229136	37.0	35.0	37.0	35.0	37.0
7	36.12904078817898	37.0	35.0	37.0	35.0	37.0
8	35.90488394846608	37.0	35.0	37.0	35.0	37.0
9	37.60156196721859	39.0	38.0	39.0	35.0	39.0
10	37.47561087629103	39.0	37.0	39.0	35.0	39.0
11	37.3818893637868	39.0	37.0	39.0	35.0	39.0
12	37.15105070582881	39.0	37.0	39.0	34.0	39.0
13	36.99348380066164	39.0	37.0	39.0	33.0	39.0
14	37.98302754144996	40.0	37.0	41.0	33.0	41.0
15	38.10411444683981	40.0	37.0	41.0	33.0	41.0
16	38.22921517514548	40.0	37.0	41.0	34.0	41.0
17	38.28789645895643	40.0	37.0	41.0	34.0	41.0
18	38.3103607847831	40.0	37.0	41.0	34.0	41.0
19	38.266183681109844	40.0	37.0	41.0	34.0	41.0
20	38.213410399475585	40.0	36.0	41.0	34.0	41.0
21	38.154264965199154	40.0	36.0	41.0	34.0	41.0
22	38.105129036612915	40.0	36.0	41.0	34.0	41.0
23	37.90430149878152	40.0	36.0	41.0	34.0	41.0
24	37.69857846091324	39.0	35.0	41.0	33.0	41.0
25	37.62920153594139	39.0	35.0	41.0	33.0	41.0
26	37.75462375977186	39.0	35.0	41.0	34.0	41.0
27	37.78640519291819	40.0	35.0	41.0	34.0	41.0
28	37.757507477206524	40.0	36.0	41.0	34.0	41.0
29	37.76581417001964	40.0	36.0	41.0	34.0	41.0
30	37.66677823906741	40.0	36.0	41.0	33.0	41.0
31	37.57840037187707	40.0	35.0	41.0	33.0	41.0
32	37.46328827292882	39.0	35.0	41.0	33.0	41.0
33	37.4125796606065	39.0	35.0	41.0	33.0	41.0
34	37.295585908197026	39.0	35.0	41.0	33.0	41.0
35	37.17941050803252	39.0	35.0	41.0	33.0	41.0
36	37.092736149597016	39.0	35.0	41.0	32.0	41.0
37	37.05260766272753	39.0	35.0	41.0	32.0	41.0
38	36.98514300983832	39.0	35.0	41.0	32.0	41.0
39	36.92608525615956	39.0	35.0	41.0	32.0	41.0
40	36.86714023467781	39.0	35.0	41.0	32.0	41.0
41	36.78341431030859	39.0	35.0	41.0	32.0	41.0
42	36.74987648169772	39.0	35.0	41.0	32.0	41.0
43	36.684658122215616	39.0	35.0	41.0	32.0	41.0
44	36.56948687365782	39.0	35.0	41.0	31.0	41.0
45	36.57469273517537	39.0	35.0	41.0	31.0	41.0
46	36.47471597749253	38.0	35.0	40.0	31.0	41.0
47	36.39154327594198	38.0	35.0	40.0	31.0	41.0
48	36.328576776889065	38.0	35.0	40.0	31.0	41.0
49	36.28906205420331	38.0	35.0	40.0	31.0	41.0
50	36.22299333209931	38.0	35.0	40.0	31.0	41.0
51	35.19913516312071	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	17.0
10	26.0
11	29.0
12	22.0
13	22.0
14	24.0
15	56.0
16	105.0
17	197.0
18	325.0
19	641.0
20	1085.0
21	1753.0
22	2604.0
23	3636.0
24	5065.0
25	6583.0
26	8208.0
27	10025.0
28	11643.0
29	14318.0
30	18359.0
31	24515.0
32	33256.0
33	50792.0
34	101924.0
35	127942.0
36	106307.0
37	171942.0
38	309059.0
39	426202.0
40	339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76528460704479	22.0034459866642	26.54711022842883	10.684159177862181
2	31.64852049429589	24.188710914285952	25.30072357369415	18.862045017724007
3	31.450682447318574	24.79739519037128	26.561236546943217	17.19068581536693
4	29.42122455001065	25.277690019860348	25.66967796169054	19.631407468438464
5	24.82683081959091	29.852738348570735	24.777562674230396	20.542868157607963
6	27.15836925222368	32.686074233455855	24.02664098413816	16.128915530182304
7	79.497631927985	6.158796521167906	8.580799062513481	5.76277248833361
8	79.52497992392664	4.464403764977029	9.229287546432444	6.781328764663884
9	73.25867028894237	7.677967257559668	11.520534656800049	7.542827796697921
10	41.38315446955326	25.359316481029676	17.956151350629142	15.30137769878792
11	32.555875504685346	23.051298716662235	23.699648025029333	20.693177753623086
12	28.637039903022476	20.85267293606136	30.31946356175289	20.190823599163277
13	25.119586592940742	24.585987527087042	30.616603364986496	19.677822514985728
14	19.695984924504224	27.816669612549184	31.111789978525213	21.375555484421387
15	18.181963683531496	24.569773575294672	35.941877506029776	21.306385235144056
16	21.916670030075835	24.816114302097237	30.761554702254784	22.505660965572144
17	22.193698966064826	24.555647256780286	26.47689616251251	26.773757614642378
18	24.066166840868068	23.885099447890585	30.3065898232053	21.742143888036054
19	23.224293927631496	25.692224401092805	26.88154907956249	24.20193259171321
20	24.30130393574543	27.607975872526332	27.702684835571045	20.388035356157197
21	23.29569098573868	25.98414512113144	30.28265162828437	20.43751226484551
22	21.555300709656137	24.22761048103246	28.434330711729856	25.78275809758155
23	20.103073413711854	26.312112309103338	29.324706304791675	24.26010797239314
24	22.16697726010658	24.457806843818588	29.162218847988285	24.212997048086546
25	20.28003512790929	27.23331528690344	27.276738059085588	25.20991152610168
26	20.575713587848306	27.605540300368673	27.33811447745843	24.480631634324588
27	20.759146965207503	27.475271983822235	28.35012950285101	23.41545154811925
28	18.887235792611726	26.928590416093144	28.843437246439542	25.340736544855584
29	20.694151982486147	28.236701428080334	26.930956400474866	24.138190188958646
30	23.50501101574493	27.21494411405715	26.861925326749404	22.41811954344852
31	25.780252937647962	28.024876238140507	23.258670288942362	22.936200535269172
32	25.17950166801899	29.25727574991267	22.847128182075025	22.71609439999332
33	25.51407969470451	26.39359959472079	24.961970280452654	23.130350430122043
34	22.862785431659933	25.37386032619966	27.10158562706241	24.661768615078
35	23.200564496038368	28.239902465773252	25.066282356576114	23.493250681612267
36	25.603778337882055	27.16275328210745	25.87266550408689	21.360802875923603
37	23.750864628115966	27.241109117807927	26.600762403673123	22.407263850402984
38	24.099638561091805	28.13531203854606	24.767820385599784	22.99722901476235
39	24.496706410565096	23.91425672600648	27.156420794497553	24.43261606893087
40	26.080663366350414	22.959303676878907	27.124201654240608	23.83583130253007
41	22.093979683152938	24.845410755765	25.996044630815973	27.064564930266087
42	23.55748019879836	23.778351799609474	28.871759471244246	23.792408530347924
43	23.862970535143916	23.85782103972488	28.903839435949326	23.375368989181887
44	22.952205723733744	24.64680724325242	26.712868310701072	25.688118722312765
45	23.459500610284795	23.965403741317186	25.14408149006913	27.431014158328892
46	24.99057085636109	23.86081331408999	26.480375551309155	24.668240278239765
47	20.802500149613717	24.716047080305685	30.31876768399356	24.162685086087038
48	22.208729925666336	23.909733520570843	29.425330228790692	24.45620632497213
49	23.89894741530124	21.59176470424499	30.84276363676851	23.66652424368526
50	22.160714360272618	22.848589525369615	28.759931915320024	26.230764199037743
51	21.26901659946807	22.730429481835504	25.846709263663907	30.15384465503252
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1035.0
1	1384.5
2	1734.0
3	2305.5
4	2877.0
5	2290.5
6	1704.0
7	1855.0
8	2006.0
9	2087.5
10	2169.0
11	2247.0
12	2325.0
13	2515.0
14	2705.0
15	2801.0
16	2897.0
17	2821.0
18	2745.0
19	3029.5
20	3314.0
21	3358.5
22	3403.0
23	3535.0
24	3667.0
25	5029.5
26	7805.0
27	9218.0
28	11009.5
29	12801.0
30	14883.0
31	16965.0
32	17956.5
33	18948.0
34	24736.5
35	30525.0
36	32114.5
37	33704.0
38	36810.5
39	39917.0
40	47359.5
41	54802.0
42	64601.0
43	74400.0
44	86300.0
45	98200.0
46	114686.0
47	131172.0
48	151630.5
49	172089.0
50	170282.5
51	168476.0
52	143159.5
53	117843.0
54	103193.0
55	88543.0
56	80896.0
57	73249.0
58	69796.5
59	66344.0
60	60459.5
61	54575.0
62	46764.5
63	38954.0
64	34934.5
65	30915.0
66	26864.0
67	22813.0
68	19549.0
69	16285.0
70	14352.5
71	12420.0
72	9229.5
73	6039.0
74	5171.5
75	3214.0
76	2124.0
77	1504.0
78	884.0
79	935.0
80	986.0
81	654.5
82	323.0
83	250.5
84	178.0
85	107.0
86	36.0
87	28.5
88	21.0
89	13.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1437034.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.878627196344528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.27205138308986	13.419121999718325
2	12.883713293773754	5.379884935305804
3	6.196189472639375	3.8810379011145626
4	3.6480030520893387	3.046611829428012
5	2.320956943044639	2.422919737629823
6	1.550439173767186	1.9422624899816103
7	1.1706023095843678	1.7108398451894324
8	0.8638998175504826	1.4429633780501263
9	0.642473381378799	1.207256599204454
>10	4.307905969378348	18.04193855338363
>50	1.2987929531034965	20.088599931800548
>100	0.8291374436716988	23.343600227008984
>500	0.011118055928674252	1.534096244288217
>1k	0.004042929428608819	1.569337760102257
>5k	6.73821571434803E-4	0.9695285677942361
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	7161	0.4983180634557011	No Hit
GCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	6622	0.4608102522278526	No Hit
CTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCT	4585	0.3190599526524773	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	3001	0.2088329155747185	No Hit
ACTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	2249	0.15650290807315623	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCC	1606	0.1117579681482832	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	1594	0.11092291483708806	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	1562	0.10869610600723434	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTG	1540	0.10716517493670992	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.958777593292852E-5	0.0	0.0	0.3336037978224593	0.0
2	6.958777593292852E-5	0.0	0.0	1.714503623435493	0.0
3	6.958777593292852E-5	0.0	0.0	2.478577403179048	0.0
4	6.958777593292852E-5	0.0	0.0	3.350025121187112	0.0
5	6.958777593292852E-5	0.0	0.0	5.667785174185162	0.0
6	6.958777593292852E-5	0.0	0.0	6.821411323601251	0.0
7	6.958777593292852E-5	0.0	0.0	8.109272292791958	0.0
8	6.958777593292852E-5	0.0	0.0	9.912430742766002	0.0
9	6.958777593292852E-5	0.0	0.0	10.547697549257707	0.0
10	6.958777593292852E-5	0.0	0.0	11.999089791890798	0.0
11	6.958777593292852E-5	0.0	0.0	14.313300868316269	0.0
12	6.958777593292852E-5	0.0	0.0	16.057379296523255	0.0
13	6.958777593292852E-5	0.0	0.0	16.736486401852705	0.0
14	6.958777593292852E-5	0.0	0.0	17.00704367467993	0.0
15	1.3917555186585703E-4	0.0	0.0	17.52004475885748	0.0
16	1.3917555186585703E-4	0.0	0.0	18.60150838463112	0.0
17	1.3917555186585703E-4	0.0	0.0	19.88651625500858	0.0
18	1.3917555186585703E-4	0.0	0.0	21.319189385915713	0.0
19	1.3917555186585703E-4	0.0	0.0	22.169273656712367	0.0
20	2.7835110373171406E-4	0.0	0.0	23.00147386909426	0.0
21	2.7835110373171406E-4	0.0	0.0	23.968465603458235	0.0
22	2.7835110373171406E-4	0.0	0.0	24.93169959792183	0.0
23	2.7835110373171406E-4	0.0	0.0	25.90335371327331	0.0
24	2.7835110373171406E-4	0.0	0.0	26.6505872512411	0.0
25	3.479388796646426E-4	0.0	0.0	27.31856031242128	0.0
26	3.479388796646426E-4	0.0	0.0	27.956262691070634	0.0
27	3.479388796646426E-4	0.0	0.0	28.63996258961166	0.0
28	3.479388796646426E-4	0.0	0.0	29.274394342792167	0.0
29	3.479388796646426E-4	0.0	0.0	29.937078733001446	0.0
30	3.479388796646426E-4	0.0	0.0	30.748263437051595	0.0
31	3.479388796646426E-4	0.0	0.0	31.398213264265145	0.0
32	3.479388796646426E-4	0.0	0.0	32.03368883408465	0.0
33	3.479388796646426E-4	0.0	0.0	32.661648924103396	0.0
34	3.479388796646426E-4	0.0	0.0	33.28216312209732	0.0
35	3.479388796646426E-4	0.0	0.0	33.983538315725305	0.0
36	3.479388796646426E-4	0.0	0.0	34.58408082202648	0.0
37	3.479388796646426E-4	0.0	0.0	35.20014140236069	0.0
38	3.479388796646426E-4	0.0	0.0	35.88912997187262	0.0
39	3.479388796646426E-4	0.0	0.0	36.77491277172287	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAC	20	7.033876E-4	45.000004	33
AGCCGAA	20	7.033876E-4	45.000004	45
CCCCTAG	30	2.1658816E-6	45.000004	14
CGAACTA	20	7.033876E-4	45.000004	21
TACCCGT	20	7.033876E-4	45.000004	33
CGAACGC	30	2.1658816E-6	45.000004	22
CGTATCG	20	7.033876E-4	45.000004	40
CTCGTAG	30	2.1658816E-6	45.000004	1
GCGAAGT	20	7.033876E-4	45.000004	2
CGGCACA	20	7.033876E-4	45.000004	28
CGTCTAT	40	6.8175723E-9	45.000004	3
CGTCTAA	30	2.1658816E-6	45.000004	29
CCGCTAT	20	7.033876E-4	45.000004	26
TACGCGT	20	7.033876E-4	45.000004	22
CGCTATA	30	2.1658816E-6	45.000004	22
CGTAGGC	20	7.033876E-4	45.000004	33
TATTACG	20	7.033876E-4	45.000004	1
CGTACTC	20	7.033876E-4	45.000004	36
AATTACG	20	7.033876E-4	45.000004	1
TTACGGT	30	2.1658816E-6	45.000004	24
>>END_MODULE