SRR2935045.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935045.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1105299
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	11659	1.0548276982065488	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	9398	0.850267665129526	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	7303	0.6607261926410862	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	5074	0.45906130377391097	TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	3807	0.3444316877152698	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	2281	0.20636949820817713	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	2047	0.18519875617366885	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	2005	0.18139887939824428	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1840	0.1664707920662192	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1711	0.1547997419702723	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC	1693	0.1531712233522332	No Hit
AAAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	1605	0.14520957677515314	No Hit
GGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1588	0.14367153141367178	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	1475	0.13344805342264854	No Hit
GGCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	1282	0.11598671490700707	No Hit
AGCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	1212	0.1096535869479661	No Hit
TGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1193	0.10793459507336929	No Hit
TTGTCTGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAAAAGAAAGAAA	1173	0.10612512994221472	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGAT	20	7.033167E-4	45.000004	36
AGTCCGC	20	7.033167E-4	45.000004	39
AGTCCAT	20	7.033167E-4	45.000004	25
TCGCACG	35	1.2118653E-7	45.000004	1
GCGACCG	20	7.033167E-4	45.000004	10
TCCGCAA	20	7.033167E-4	45.000004	39
CGTATAC	20	7.033167E-4	45.000004	24
ATAGGCG	20	7.033167E-4	45.000004	1
CTATCCA	20	7.033167E-4	45.000004	31
GAGTCGA	20	7.033167E-4	45.000004	20
GCGAAAG	55	1.8189894E-12	45.000004	1
CGGTTTA	20	7.033167E-4	45.000004	19
TTTCGCG	20	7.033167E-4	45.000004	1
TTTCGCA	20	7.033167E-4	45.000004	15
CGTTGAT	20	7.033167E-4	45.000004	25
ATTACGC	40	6.8139343E-9	45.000004	12
TAGCGAT	20	7.033167E-4	45.000004	29
GATCCGT	20	7.033167E-4	45.000004	7
TTGTCCG	20	7.033167E-4	45.000004	1
TTGTCAA	20	7.033167E-4	45.000004	30

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content